期刊
NUCLEIC ACIDS RESEARCH
卷 39, 期 4, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkq1182
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资金
- Amlov's Foundation
- Foundation Edit Jacobson
- Frimurare Foundation
- Grant Agency of the Academy of Science [IAA50520809, IAA500970904]
- Grant Agency of the Czech Republic [P303/10/1338]
- Hjarnfonden
- LUA/ALF Goteborg
- Region of Vastra Gotaland (RUN)
- Sigurd and Elsa Goljes Memory Foundation
- Socialstyrelsens Foundation
- Swedish Stroke Foundation
- Swedish Society for Medicine
- Swedish Society for Medical Research
- Swedish Medical Research Council [11548, 2005-5174]
- Trygg-Hansa
- Torsten and Ragnar Soderberg Foundations
- Wilhelm and Martina Lundgren's Research Foundation
Single-cell gene expression levels show substantial variations among cells in seemingly homogenous populations. Astrocytes perform many control and regulatory functions in the central nervous system. In contrast to neurons, we have limited knowledge about functional diversity of astrocytes and its molecular basis. To study astrocyte heterogeneity and stem/progenitor cell properties of astrocytes, we used single-cell gene expression profiling in primary mouse astrocytes and dissociated mouse neurosphere cells. The transcript number variability for astrocytes showed lognormal features and revealed that cells in primary cultures to a large extent co-express markers of astrocytes and neural stem/progenitor cells. We show how subpopulations of cells can be identified at single-cell level using unsupervised algorithms and that gene correlations can be used to identify differences in activity of important transcriptional pathways. We identified two subpopulations of astrocytes with distinct gene expression profiles. One had an expression profile very similar to that of neurosphere cells, whereas the other showed characteristics of activated astrocytes in vivo.
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