Article
Pharmacology & Pharmacy
Serra Gurcan, Nicolas Tsapis, Franceline Reynaud, Stephanie Denis, Juliette Vergnaud, Ozgen Ozer, Elias Fattal
Summary: The study proposes a novel drug delivery system combining two different anti-inflammatory approaches. The system consists of cationic dexamethasone palmitate nanoparticles and TNF-alpha siRNA. Results show that the system is effective in internalizing and localizing within macrophages, leading to enhanced TNF-alpha inhibition, offering a potential alternative strategy for treating inflammatory diseases.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Chemistry, Multidisciplinary
Jia-Xin An, Zi-Yi Han, You-Teng Qin, Chu-Xin Li, Jin-Lian He, Xian-Zheng Zhang
Summary: This study reports a strategy of attaching bacteria to macrophages, which sustainably stimulates the polarization of macrophages and reduces tumor progression in an immunosuppressive tumor microenvironment without any side effects. This research opens a new avenue for the development of cell therapies.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Annabelle Biscans, Socheata Ly, Nicholas McHugh, David A. Cooper, Anastasia Khvorova
Summary: The study demonstrates the importance of lipid conjugation in improving siRNA endosomal escape and tissue distribution. Modulating the nature of the conjugate can affect the intratissue distribution and non-specific gene modulation of siRNA.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Multidisciplinary Sciences
Feng Li, Wenting Yu, Jiaojiao Zhang, Yuhang Dong, Xiaohui Ding, Xinhua Ruan, Zi Gu, Dayong Yang
Summary: DNA nanostructures have been used as carriers for gene delivery, but spatiotemporally programmable assembly of DNA under nanoconfinement is challenging due to DNA complexity. A strategy using cascade hybridization chain reaction of DNA hairpins in polymeric nanoframework enables precise siRNA delivery. The nanoframework provides stability and ease of functionalization, addressing DNA complexity issues. Phenylboronate installation on nanoframework enhances cellular uptake and lysosomal escape.
NATURE COMMUNICATIONS
(2021)
Article
Pharmacology & Pharmacy
Hongqian Zhang, Feng Ding, Zongwei Zhu, Qian Sun, Chuanxu Yang
Summary: Small interfering RNA (siRNA) is a promising strategy for specific gene silencing in anti-inflammatory therapy. However, finding efficient carriers for delivering anti-inflammatory siRNA to macrophages remains challenging. In this study, we developed ionizable lipid nanoparticles (LNPs) with synthetic lipid-like materials to deliver siRNA to macrophages. By modifying the structure of synthetic lipid-like materials, we identified a potent LNP (1O14-LNP) that effectively transfects macrophages by promoting internalization and escape from endosomes. 1O14-LNP successfully delivered anti-inflammatory siRNA against interleukin-10 (siIL-10), resulting in significant downregulation of IL-10 expression in LPS-activated macrophages.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Multidisciplinary
Yudong Song, Yanhua Wu, Lu Xu, Ting Jiang, Cui Tang, Chunhua Yin
Summary: Delivery of siRNA into cells remains a challenge, but understanding the cellular uptake and processing of siRNA systems can enhance the development of siRNA-based therapies. Research indicates that caveolae-mediated endocytosis plays a crucial role in efficient siRNA delivery, along with the Golgi complex and endoplasmic reticulum. Additionally, syntaxin6 and Niemann-Pick C1 protein are essential regulators in the delivery of siRNA to macrophages.
Article
Biophysics
Urushi Rehman, Neha Parveen, Afsana Sheikh, Mohammed A. S. Abourehab, Amirhossein Sahebkar, Prashant Kesharwani
Summary: Ovarian cancer is a major cause of death in women, and there is a need for innovative treatment approaches. RNA interference and nanotechnology can improve ovarian cancer therapy and overcome chemo-resistance. Nanoparticles are used to transport siRNA molecules to the targeted site, enhancing the therapeutic potential.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Multidisciplinary Sciences
Rakan El-Mayta, Marshall S. Padilla, Margaret M. Billingsley, Xuexiang Han, Michael J. Mitchell
Summary: Lipid nanoparticles (LNPs) have been successfully used in the development of COVID-19 mRNA vaccines, demonstrating the efficacy of mRNA-LNP therapeutics and opening up possibilities for future clinical applications. LNPs protect mRNA from degradation and facilitate intracellular delivery. In vitro and in vivo experiments evaluate the transfection efficiency of LNPs in cells and the luminescent flux in mice, providing representative results for their characteristics and efficacy.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Chemistry, Multidisciplinary
Ling Ding, Siyuan Tang, Weimin Tang, Deanna D. Mosley, Ao Yu, Diptesh Sil, Svetlana Romanova, Kristina L. Bailey, Daren L. Knoell, Todd A. Wyatt, David Oupicky
Summary: This study reports a method using PFOB nanoemulsions to improve the delivery efficiency of siRNA in the treatment of pulmonary fibrosis. The results show that the nanoemulsions can effectively treat pulmonary fibrosis and inhibit the progression of the disease.
Article
Multidisciplinary Sciences
Yuan Rui, David R. Wilson, Stephany Y. Tzeng, Hannah M. Yamagata, Deepti Sudhakar, Marranne Conge, Cynthia A. Berlinicke, Donald J. Zack, Anthony Tuesca, Jordan J. Green
Summary: Nanoparticle-based mRNA therapeutics show great promise, but cellular internalization and endosomal escape pose challenges for cytosolic delivery. Researchers developed a dual nanoparticle uptake and endosomal disruption assay, which can detect cellular internalization and endosomal disruption. The study found that this assay has higher predictive capacity for mRNA delivery compared to conventional polymer and nanoparticle characteristics.
Article
Biochemistry & Molecular Biology
Olivia L. Lanier, Joseph M. Ficarrotta, Isaac Adjei, Dayita Wable, Camryn Lewis, Christopher Nacea, Blanka Sharma, Jon Dobson, Peter McFetridge
Summary: Researchers have developed a magnetically triggerable polymeric microparticle platform for on-demand release of complex human placental proteins, aiming to provide a more comprehensive regenerative response. The experiments demonstrate that the proteins retain function after being magnetically released.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Arkady S. Abdurashitov, Pavel I. Proshin, Gleb B. Sukhorukov
Summary: This study introduces a novel and scalable approach for manufacturing microparticles with defined structures and sizes. The method involves depositing polymer layers on a low surface energy substrate, cutting the layered structure into desired-size blanks using a laser, and sealing the blanks by applying heat. This approach addresses the challenges faced by conventional methods and shows promise for various applications.
Review
Pharmacology & Pharmacy
Md Abdus Subhan, Vladimir P. Torchilin
Summary: Nanobiopolymers, such as chitosan, gelatin, hyaluronic acid, polyglutamic acid, lipids, peptides, exosomes, etc., have favorable stimuli-responsive properties and can be used to improve siRNA delivery platforms for overcoming physiological difficulties in delivering siRNA drugs to solid tumors, including breast cancer cells. These biopolymeric siRNA drugs can protect drugs from pH degradation, extracellular trafficking, and nontargeted binding sites, making them suitable for controlled-release drug internalization. This review discusses the utilization of various biopolymeric compounds as siRNA drug delivery systems for multidrug-resistant solid tumors, including breast cancers.
Article
Chemistry, Multidisciplinary
Chunhui Li, Junhui Zhou, Yidi Wu, Yanliang Dong, Lili Du, Tongren Yang, Yongheng Wang, Shuai Guo, Mengjie Zhang, Abid Hussain, Haihua Xiao, Yuhua Weng, Yong Huang, Xiaoxia Wang, Zicai Liang, Huiqing Cao, Yongxiang Zhao, Xing-Jie Liang, Anjie Dong, Yuanyu Huang
Summary: The study synthesized a series of quaternary ammonium-based amphiphilic triblock polymers with a pH-sensitive hydrophobic core that can rapidly release internalized siRNA in endosomal pH environment, leading to enhanced gene silencing activity and significant therapeutic effects in tumor models.
Article
Biochemistry & Molecular Biology
Beinuo Wang, Ruijie Zhang, Yao Wang, Hang Qian, Di Wu, Binfeng He, Hu Liao
Summary: Overcoming cisplatin-based drug resistance in lung cancer remains a challenge globally. Recent studies have shown that Rab GTPases play a role in multiple aspects of tumor progression, including drug resistance. In this study, a nanosystem was developed using Rab26 siRNA-loaded nanoparticles, which effectively transfected cisplatin-resistant lung cancer cells and induced apoptosis while inhibiting autophagy disruption. The combination therapy of siRab26 knockdown with cisplatin improved antitumor therapy in vitro and in vivo. These findings suggest that siRNP is an effective platform for lung cancer therapy in cases of drug resistance.
Article
Biochemistry & Molecular Biology
Anandaroop Mukhopadhyay, Jocelyn Wright, Shawna Shirley, David A. Canton, Christoph Burkart, Richard J. Connolly, Jean S. Campbell, Robert H. Pierce
Article
Oncology
Ingunn M. Stromnes, Adam L. Burrack, Ayaka Hulbert, Patrick Bonson, Cheryl Black, J. Scott Brockenbrough, Jackson F. Raynor, Ellen J. Spartz, Robert H. Pierce, Philip D. Greenberg, Sunil R. Hingorani
CANCER IMMUNOLOGY RESEARCH
(2019)
Review
Pharmacology & Pharmacy
Ammu Prasanna Kumar, Sungmun Lee, Suryani Lukman
CURRENT DRUG TARGETS
(2019)
Article
Oncology
Samantha K. Greaney, Alain P. Algazi, Katy K. Tsai, Kathryn T. Takamura, Lawrence Chen, Christopher G. Twitty, Li Zhang, Alan Paciorek, Robert H. Pierce, Mai H. Le, Adil Daud, Lawrence Fong
CANCER IMMUNOLOGY RESEARCH
(2020)
Article
Multidisciplinary Sciences
Sungmun Lee, Myung Chul Choi, Kenana Al Adem, Suryani Lukman, Tae-Yeon Kim
SCIENTIFIC REPORTS
(2020)
Article
Materials Science, Biomaterials
Fahad Alam, Mohamed Elsherif, Bader AiQattan, Ahmed Salih, Sung Mun Lee, Ali K. Yetisen, Seongjun Park, Haider Butt
Summary: The study demonstrated the fabrication of personalized smart contact lenses using additive manufacturing, which have built-in sensing capabilities through microchannels and holographic laser ablation. 3D printing allows for customized ophthalmic devices and integration with optical sensors.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Review
Oncology
James C. Lee, Michael D. Green, Laura A. Huppert, Christine Chow, Robert H. Pierce, Adil Daud
Summary: Liver metastases have a significant impact on the effectiveness of immune checkpoint inhibitors in patients with solid-tumor malignancies. Multiple clinical and translational studies have shown that liver metastasis leads to systemic immune suppression through the generation of suppressive macrophages and activation of regulatory T-cells. Various interventions, such as radiation therapy and combination checkpoint blockade, have been reported to address liver immune suppression.
CLINICAL CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Sungmun Lee, Aya Shanti
Summary: The study found that different pH environments have distinct effects on triple-negative breast cancer cells. Extreme acidic and basic conditions inhibit cell growth, induce cell death, increase ROS levels, while slightly acidic conditions promote cell growth and alter the cell cycle. Furthermore, the pH history of cells does not significantly affect their subsequent growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Peter H. Goff, Laura Riolobos, Bonnie J. LaFleur, Matthew B. Spraker, Y. David Seo, Kimberly S. Smythe, Jean S. Campbell, Robert H. Pierce, Yuzheng Zhang, Qianchuan He, Edward Y. Kim, Stephanie K. Schaub, Gabrielle M. Kane, Jose G. Mantilla, Eleanor Y. Chen, Robert Ricciotti, Matthew J. Thompson, Lee D. Cranmer, Michael J. Wagner, Elizabeth T. Loggers, Robin L. Jones, Erin Murphy, Wendy M. Blumenschein, Terrill K. McClanahan, Jon Earls, Kevin C. Flanagan, Natalie A. LaFranzo, Teresa S. Kim, Seth M. Pollack
Summary: This study aims to characterize the changes in the soft-tissue sarcoma (STS) tumor immune microenvironment induced by standard neoadjuvant therapy and provides insights for neoadjuvant immunotherapy trial design. The results show that neoadjuvant therapy significantly increases immune cell infiltration in the tumor and affects the expression of immune-related genes and cytokines. These findings inform the incorporation of immune checkpoint inhibitors and novel immunotherapies into the neoadjuvant setting for STSs.
CLINICAL CANCER RESEARCH
(2022)
Article
Materials Science, Biomaterials
Selwa Boularaoui, Aya Shanti, Michele Lanotte, Shaohong Luo, Sarah Bawazir, Sungmun Lee, Nicolas Christoforou, Kamran A. Khan, Cesare Stefanini
Summary: The study optimized GelMA bioinks through concentration and cross-linking time, enhancing printability and biological properties by adding gold nanoparticles (AuNPs) or two-dimensional transition metal carbides (MXene nanosheets). These improvements led to enhanced differentiation of encapsulated cells and enabled printing of highly viable stable constructs.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Deema Islayem, Fatima Ba Fakih, Sungmun Lee
Summary: Excessive production of reactive oxygen species (ROS) is implicated in aging-related disorders. However, monitoring ROS in vivo remains challenging due to their short half-lives. In this study, three colorimetric methods were compared for their specificity and sensitivity in detecting malondialdehyde (MDA), a biomarker of lipid oxidation induced by ROS. The results showed that the MPI method was sensitive enough to detect MDA in diseased conditions.
Article
Materials Science, Biomaterials
Nouran Farid, Aibobek Seitak, Vincent Chan, Sungmun Lee
Summary: Oxidative stress and overproduction of reactive oxygen species (ROS) play a crucial role in the development of inflammatory bowel diseases. Catalase, which can scavenge hydrogen peroxide (one of the ROS produced in cellular metabolism), has great therapeutic potential. However, its in vivo application for ROS scavenging, especially through oral administration, is currently limited. This study introduced an alginate-based oral drug delivery system that effectively protected catalase from the harsh conditions of the gastrointestinal tract, released it in a pH-dependent manner, and enhanced its absorption via specialized epithelial cells in the small intestine.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Gounhanul Shin, Kunn Hadinoto, Sungmun Lee, Jin-Won Park
Summary: The binding affinity between TGF-beta 1 and its receptors was measured using an atomic force microscope. The results showed strong and high binding between them, with little dissociation occurring.
Article
Oncology
Robert H. I. Andtbacka, Yan Wang, Robert H. Pierce, Jean S. Campbell, Melinda Yushak, Mohammed Milhem, Merrick Ross, Katie Niland, Robert D. Arbeit, Sudha Parasuraman, Kris Bickley, Cecilia C. S. Yeung, Lauri D. Aicher, Kimberly S. Smythe, Lu Gan
Summary: The study found that treatment with single-agent mavorixafor can increase CD8+ T-cell infiltration, granzyme B signal, antigen presentation machinery, and both tumor inflammatory signature (TIS) and IFNγ gene expression signature scores. When mavorixafor was combined with pembrolizumab, the increases in the key serum cytokines CXCL9 and CXCL10 were further enhanced. Reported adverse events included diarrhea, fatigue, maculopapular rash, and dry eye, all of which were ≤ grade 3.
CANCER RESEARCH COMMUNICATIONS
(2022)
Article
Nanoscience & Nanotechnology
Fatima Ba Fakih, Aya Shanti, Cesare Stefanini, Sungmun Lee
ACS APPLIED NANO MATERIALS
(2020)
