Article
Cell Biology
Shalu Sharma, Ananda Kishore Mukherjee, Shuvra Shekhar Roy, Sulochana Bagri, Silje Lier, Meenakshi Verma, Antara Sengupta, Manish Kumar, Gaute Nesse, Deo Prakash Pandey, Shantanu Chowdhury
Summary: The study reveals that the telomere-repeat-binding-factor TRF2 binds to G-quadruplexes in the hTERT promoter, leading to H3K27 trimethylation and repression of hTERT in both cancer and normal cells. Two highly recurrent hTERT promoter mutations found in many cancers disrupt G-quadruplexes, resulting in loss of TRF2 binding.
Article
Biochemistry & Molecular Biology
Di Bai, Song-Wang Shan, Xin Zhang, Yan Li, Jie Xie, Wen-Qiang Wu
Summary: In this study, the folding, dynamics induced by environment, and protein-catalyzed unfolding of plant telomeric G4s were comprehensively studied. It was found that various plant telomeric sequences can fold into G4 structures, and the stability of G4s in dilute solution is decreased by both 5' and 3' ssDNA. Molecular crowding promotes the formation of parallel structures for three-layer G4s and increases the stability of all selected G4s. AtRecQ2 helicase can resolve the stable parallel structure of typical plant telomeric G4 in crowded solution, while ssDNA binding protein AtRPA cannot. Furthermore, AtRecQ2 unwinds the structure more efficiently in the presence of AtRPA. These results expand our understanding of the structures and dynamics of plant telomeric G4s.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Nikita Kundu, Taniya Sharma, Sarvpreet Kaur, Aman Kumar Mahto, Rikeshwer Prasad Dewangan, J. Shankaraswamy, Sarika Saxena
Summary: This study provides the first evidence of human telomeric G4 destabilization upon peptide binding, which will open new research avenues for treating DNA-related diseases.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Taniya Sharma, Nikita Kundu, Sarvpreet Kaur, Vibha Tandon, J. Shankaraswamy, Sarika Saxena
Summary: This study provides evidence of human telomeric G4 destabilization upon peptide binding in dilute and cell-mimicking conditions, suggesting the potential therapeutic targets for diseases with over-representation of G4 motifs.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Chemistry, Medicinal
Domen Oblak, Hadzi, Crtomir Podlipnik, Jurij Lah
Summary: The authors investigated the (un)folding and binding process of a human telomeric fragment with ligands through calorimetry and spectroscopy. The results showed that the presence of specific ligands can alter the topology of G-quadruplex nucleic acids. By analyzing thermodynamic parameters and conducting molecular modeling, the driving forces behind the topological transformations were clarified.
Article
Biochemistry & Molecular Biology
Valentina Pirota, Chiara Platella, Domenica Musumeci, Alessandra Benassi, Jussara Amato, Bruno Pagano, Giorgio Colombo, Mauro Freccero, Filippo Doria, Daniela Montesarchio
Summary: NDI-5 has shown promising potential as a ligand for multimeric G4 structures, selectively targeting them over duplex DNA. Experimental analyses including CD, gel electrophoresis, ITC, and fluorescence spectroscopy suggest that NDI-5 effectively binds and stabilizes tel46 G4, favoring a hybrid folding in K+-containing buffer.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Pegah Fazelifar, Anne Cucchiarini, Zahra Khoshbin, Jean-Louis Mergny, Sakineh Kazemi Noureini
Summary: G-rich sequences can fold into G-quadruplexes and have potential implications in anti-cancer drug design. A study found that palmatine can increase the stability of different G-rich sequences and has the strongest interaction with TRF2.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Samar Ali, Emilia Puig Lombardi, Deepanjan Ghosh, Tao Jia, Geraldine Vitry, Lina Saker, Joel Poupon, Marie-Paule Teulade-Fichou, Alain Nicolas, Arturo Londono-Vallejo, Sophie Bombard
Summary: Pt-ttpy shows dual targeting efficiency on DNA by inducing telomere dysfunction and genomic DNA damage at specific loci, while not particularly accumulating in potential G4 forming sequences.
Article
Biochemistry & Molecular Biology
Yu-Cheng Liu, Dah-Yen Yang, Sheh-Yi Sheu
Summary: G-quadruplexes have become attractive drug targets in cancer therapy, but the polymorphic nature of their structures makes understanding their conformational conversion mechanisms challenging. This study used molecular dynamics simulation to show that as salt concentration increases, the mechanism of conformational conversion shifts from multi-pathway to sequential pathways, with conformational conversion being more feasible upon K+ binding compared to Na+ binding in high-salt solutions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Luca Bertini, Valeria Libera, Francesca Ripanti, Francesca Natali, Marco Paolantoni, Andrea Orecchini, Alessandro Nucara, Caterina Petrillo, Lucia Comez, Alessandro Paciaroni
Summary: Telomeric G-quadruplexes (G4s) exhibit structural polymorphism and the dynamics of G4s are affected by conformation and complexation. In this study, the dynamics of Tel22 in different conformations and complexed with BRACO19 ligand were investigated. The results showed that hydration water played a role in mediating the effect of polymorphism and complexation on the fast dynamics of G4s.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xupeng Yu, Sean Gray, Helder C. Ferreira
Summary: The POT-3 protein in Caenorhabditis elegans is found to bind the telomeric G-strand and affects telomere length and telomeric C-circle formation, showing differences with the closely related POT-2 protein in terms of binding sites and function.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Ming-Hao Hu, Jia-Hong Lin
Summary: This study proposed a strategy to design small-molecule ligands with selective binding to multimeric G-quadruplexes (G4s), and synthesized a library of multi-aryl compounds by attaching triazole rings to the quinoxaline skeleton. Among them, QTR-3 was identified as the most promising selective ligand that may bind at the G4-G4 interface, thereby stabilizing multimeric G4s and inducing DNA damage in the telomeric region, leading to cell cycle arrest and apoptosis. Importantly, QTR-3 exhibited more significant inhibition on breast cancer cells compared to normal mammary cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Aparna Bansal, Priyanka Phogat, Shrikant Kukreti
Summary: This study reveals the formation of an intramolecular non-conventional triplex structure by Chlamydomonas reinhardtii telomere in Na+ solution, which is stabilized by the co-existence of reverse Hoogsteen and Wobble base pairing.
Article
Biochemistry & Molecular Biology
Xiaobin Ling, Yuqi Yao, Lei Ding, Jinbiao Ma
Summary: The study reveals that the nucleic acid binding protein UP1 can bind and unfold the G-quadruplexes in human telomere DNA. The RNA recognition motifs RRM1 and RRM2 in UP1 are responsible for initial binding, unfolding, and completion of G-quadruplexes unfolding, respectively. The TAG binding motif in the telomeric G-quadruplex Loop2 is critical for UP1 recognition and initiation of G-quadruplexes unfolding.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2023)
Article
Biochemistry & Molecular Biology
Valeria Libera, Francesca Ripanti, Caterina Petrillo, Francesco Sacchetti, Javier Ramos-Soriano, Maria Carmen Galan, Giorgio Schiro, Alessandro Paciaroni, Lucia Comez
Summary: Guanine-rich DNA sequences can form non-canonical nucleic acid structures called G-quadruplexes (G4s), which have implications in various fields. Ligands that interact with G4s have attracted attention for their potential in medical therapies and nanodevice applications. This study investigates the manipulation of the secondary structure of a human telomeric G4 sequence by two photosensitive ligands, demonstrating their different effects on G4 unfolding and stabilization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Psychology
William Hayward, Sarah F. Snider, George Luta, Rhonda B. Friedman, Peter E. Turkeltaub
COGNITIVE NEUROPSYCHOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Asmaa M. Baker, Qiang Fu, William Hayward, Stuart M. Lindsay, Terace M. Fletcher
NUCLEIC ACIDS RESEARCH
(2009)
Article
Multidisciplinary Sciences
Asmaa M. Baker, Qiang Fu, William Hayward, Samuel Victoria, Ilene M. Pedroso, Stuart M. Lindsay, Terace M. Fletcher
Article
Audiology & Speech-Language Pathology
Mackenzie E. Fama, Sarah F. Snider, Mary P. Henderson, William Hayward, Rhonda B. Friedman, Peter E. Turkeltaub
JOURNAL OF SPEECH LANGUAGE AND HEARING RESEARCH
(2019)
Letter
Clinical Neurology
Sara J. Hussain, William Hayward, Farah Fourcand, Christoph Zrenner, Ulf Ziemann, Ethan R. Buch, Margaret K. Hayward, Leonardo G. Cohen
Article
Psychology, Experimental
Mackenzie E. Fama, Mary P. Henderson, Sarah F. Snider, William Hayward, Rhonda B. Friedman, Peter E. Turkeltaub
CONSCIOUSNESS AND COGNITION
(2019)
Article
Audiology & Speech-Language Pathology
Mackenzie E. Fama, William Hayward, Sarah F. Snider, Rhonda B. Friedman, Peter E. Turkeltaub
BRAIN AND LANGUAGE
(2017)