Editorial Material
Biochemical Research Methods
Nina Vogt
Summary: DeepLabCut-Live! performs real-time pose estimation and enables closed-loop feedback on behavior.
Article
Biochemical Research Methods
Renna L. Nouwairi, Larissa L. Cunha, Rachelle Turiello, Orion Scott, Jeff Hickey, Scott Thomson, Stuart Knowles, Jeff D. Chapman, James P. Landers
Summary: This article describes a field-forward prototype instrument capable of ultra-fast thermal cycling for real-time PCR amplification of DNA and RNA. The instrument can complete 40 cycles of real-time PCR in under 10 minutes, an 84% reduction in time compared to standard assays.
Article
Chemistry, Analytical
Yao Fu, Li Zhang, Yu Xing, Shixiong Deng
Summary: A real-time detection method for DNA methylation was developed in this study, which provides an option for analyzing methylation levels in resource-limited environments and holds great potential for the diagnosis of methylation-related diseases.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Anuj Joshi, Harmen S. Zijlstra, Elena Liles, Carina Concepcion, Mikko Linnolahti, J. Scott McIndoe
Summary: Using electrospray ionization mass spectrometry, the real-time synthesis of methylalumoxane (MAO) was successfully tracked, revealing rapid oligomerization within a minute followed by slow aggregation. The principal activated product from benchtop synthesis matches that observed in industrial samples.
Article
Food Science & Technology
Ana Caroline de Oliveira, Aline Marien, Julie Hulin, Yordan Muhovski, Vincent Baeten, Eric Janssen, Gilbert Berben, Herve Rogez, Frederic Debode
Summary: The study compared three methods for DNA extraction from cocoa-derived products and chocolate, with the classical CTAB method proving to provide suitable DNA for PCR amplification. Seven real-time PCR tests for cocoa detection were designed, with two methods successfully meeting the performance criteria for specificity, sensitivity, and applicability in processed cocoa-derived products and chocolate. This methodology is useful for cocoa authentication and may lead to a future distinction between fine and bulk genotypes using a cost-effective and efficient approach.
Article
Nanoscience & Nanotechnology
Julija Dronina, Deivis Plausinaitis, Urte Samukaite-Bubniene, Arunas Ramanavicius
Summary: Immobilization of DNA-modifying enzymes on surfaces is a complex task in biotechnology and biosensorics. This study focuses on the real-time monitoring of T7 DNA polymerase immobilization and its analytical signals using Quartz crystal micro-balance (QCM). The research evaluates the covalent attachment of T7 DNA polymerase to self-assembled monolayers and proposes a strategy based on protecting the exonuclease active site. The findings suggest the detection of low concentrations of target DNA molecules and efficient reuse of expensive DNA enzymes.
MATERIALS TODAY NANO
(2022)
Article
Food Science & Technology
Wajeehah Wajahat, Z. R. Azaz Ahmad Azad, Sadaf Nazir, Gazia Nasir
Summary: The study evaluated the use of RT-PCR to detect adulteration of camel milk, successfully amplifying camel specific primers. The results indicated positive amplification for camel milk and its products, demonstrating the effectiveness and practicality of this method for identification.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2022)
Article
Optics
Shihua Zhou
Summary: The study introduces a real-time one-time pad DNA-chaos image encryption algorithm using multiple keys, which is simple, fast, has a large key space, and is resistant to performance attacks.
OPTICS AND LASER TECHNOLOGY
(2021)
Article
Chemistry, Physical
Ronny Frank, Dana Krinke, Christian Sonnendecker, Wolfgang Zimmermann, Heinz-Georg Jahnke
Summary: The Earth has entered the Anthropocene, characterized by widespread environmental pollution from plastics like PET. There is a pressing need for ecofriendly and cost-effective solutions for plastic recycling and reuse. By altering the dielectric properties of PET films, a monitoring system has been developed using impedance spectroscopy to analyze the enzymatic hydrolysis process of PET films with polyester hydrolases.
Article
Multidisciplinary Sciences
Fulya Ekiz Kanik, Iris Celebi, Derin Sevenler, Kahraman Tanriverdi, Nese Lortlar Unlu, Jane E. Freedman, M. Selim Unlu
Summary: In this study, a highly sensitive and multiplexed digital microarray was developed for miRNA detection using gold nanorods as labels. Real-time tracking of target molecules on the sensor surface improved sensitivity compared to traditional endpoint assays. The developed method showed potential for detecting low-abundance biomarkers in the presence of high-abundance background molecules.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Sjoerd W. Nooteboom, Yuyang Wang, Swayandipta Dey, Peter Zijlstra
Summary: A DNA-mediated nanothermometer is developed for directly monitoring the local temperature near the interface of nanomaterials. This method shows high accuracy and precision, and enables real-time tracking of interfacial temperature, providing valuable insights for understanding and controlling interfacial heating in nanomaterials.
Article
Medicine, Research & Experimental
Ymke van Der Pol, Normastuti Adhini Tantyo, Nils Evander, Anouk E. Hentschel, Birgit M. M. Wever, Jip Ramaker, Sanne Bootsma, Marieke F. Fransen, Kristiaan J. Lenos, Louis Vermeulen, Famke L. Schneiders, Idris Bahce, Jakko A. Nieuwenhuijzen, Renske D. M. Steenbergen, D. Michiel Pegtel, Norbert Moldovan, Florent Mouliere
Summary: This study demonstrates the use of Oxford Nanopore Technologies (ONT) sequencing to obtain genomic and fragmentomic data from liquid biopsies. The results show that copy number aberrations and cfDNA fragmentation patterns can be determined in less than 24 hours. Furthermore, ONT sequencing allows analysis of previously understudied cfDNA populations.
EMBO MOLECULAR MEDICINE
(2023)
Article
Chemistry, Analytical
Kim Arnold, Philippe Dehio, Jonas Lotscher, Kapil Dev Singh, Diego Garcia-Gomez, Christoph Hess, Pablo Sinues, Maria L. Balmer
Summary: In this study, a novel setup coupled with a secondary electrospray ionization-high resolution mass spectrometric (SESI-HRMS) platform was developed to analyze the real-time metabolic changes in immature and activated dendritic cells (DCs). Distinct metabolic signatures were detected in DCs treated with different supernatants of bacterial cultures, and differences in the metabolic profile of naive and activated DCs were discovered. Pathway-enrichment analysis revealed three significantly altered pathways.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yao Wu, Farshad Tehrani, Hazhir Teymourian, John Mack, Alexander Shaver, Maria Reynoso, Jonathan Kavner, Nickey Huang, Allison Furmidge, Andres Duvvuri, Yuhang Nie, Lori M. Laffel, Francis J. Doyle, Mary-Elizabeth Patti, Eyal Dassau, Joseph Wang, Netzahualcoyotl Arroyo-Curras
Summary: This study expands the ability to monitor molecular concentrations by using microneedle-supported electrochemical, aptamer-based (E-AB) sensors in interstitial fluid (ISF). The promising results obtained in vitro and in vivo lay the foundation for the translation of microneedle E-AB sensor arrays to biomedical research in preclinical animal models.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Xiaojing Chen, Shaojuan Lai, Chuxiong Zhuang, Jilei Huang, Yufei Hu
Summary: The study utilized SC-PCR to detect single-copy T-DNA plants and found that single-copy T-DNA integrations were more frequent in transgenic T-1 Arabidopsis without a vector backbone. By combining negative screening of the vector backbone and SC-PCR, they efficiently identified single-copy T-DNA plants. Additionally, the study highlighted the need to reevaluate PCR-based methods for detecting T-DNA copy number in plants containing inverted T-DNA repeats.
Editorial Material
Plant Sciences
Paloma Mas, Jaime Martinez-Garcia, Jose Luis Riechmann, Soraya Pelaz
PHYSIOLOGIA PLANTARUM
(2015)
Review
Plant Sciences
Mariana Bustamante, Jose Tomas Matus, Jose Luis Riechmann
JOURNAL OF EXPERIMENTAL BOTANY
(2016)
Article
Biotechnology & Applied Microbiology
Patricia Baldrich, Klementina Kakar, Christelle Sire, Ana Beatriz Moreno, Angelique Berger, Meritxell Garcia-Chapa, Juan Jose Lopez-Moya, Jose Luis Riechmann, Blanca San Segundo
Review
Biochemical Research Methods
Thilia Ferrier, Jose Tomas Matus, Jian Jin, Jose Luis Riechmann
CURRENT OPINION IN BIOTECHNOLOGY
(2011)
Review
Plant Sciences
Frank Wellmer, Emmanuelle Graciet, Jose Luis Riechmann
JOURNAL OF EXPERIMENTAL BOTANY
(2014)
Article
Biochemistry & Molecular Biology
Yuling Jiao, Jose Luis Riechmann, Elliot M. Meyerowitz
Article
Multidisciplinary Sciences
Kerstin Kaufmann, Frank Wellmer, Jose M. Muino, Thilia Ferrier, Samuel E. Wuest, Vijaya Kumar, Antonio Serrano-Mislata, Francisco Madueno, Pawel Krajewski, Elliot M. Meyerowitz, Gerco C. Angenent, Jose Luis Riechmann
Article
Multidisciplinary Sciences
W. Huang, P. Perez-Garcia, A. Pokhilko, A. J. Millar, I. Antoshechkin, J. L. Riechmann, P. Mas
Review
Genetics & Heredity
Frank Wellmer, Jose L. Riechmann
TRENDS IN GENETICS
(2010)
Article
Biotechnology & Applied Microbiology
Alice Pajoro, Pedro Madrigal, Jose M. Muino, Jose Tomas Matus, Jian Jin, Martin A. Mecchia, Juan M. Debernardi, Javier F. Palatnik, Salma Balazadeh, Muhammad Arif, Diarmuid S. O'Maoileidigh, Frank Wellmer, Pawel Krajewski, Jose-Luis Riechmann, Gerco C. Angenent, Kerstin Kaufmann
Article
Multidisciplinary Sciences
Norma Fabregas, Fidel Lozano-Elena, David Blasco-Escamez, Takayuki Tohge, Cristina Martinez-Andujar, Alfonso Albacete, Sonia Osorio, Mariana Bustamante, Jose Luis Riechmann, Takahito Nomura, Takao Yokota, Ana Conesa, Francisco Perez Alfocea, Alisdair R. Fernie, Ana I. Cano-Delgado
NATURE COMMUNICATIONS
(2018)
Article
Plant Sciences
Michela Osnato, Unai Cereijo, Jan Sala, Luis Matias-Hernandez, Andrea E. Aguilar-Jaramillo, Maria Rosa Rodriguez-Goberna, Jose Luis Riechmann, Manuel Rodriguez-Concepcion, Soraya Pelaz
Summary: Members of the plant-specific RAV family of transcription factors, TEM1 and TEM2, play crucial roles in regulating phase changes and abiotic stress responses in Arabidopsis thaliana. TEM overexpressing plants were hypersensitive to salt stress, while tem1 tem2 double mutants showed increased tolerance. Additionally, tem1 tem2 mutants exhibited faster germination and flowering, delayed leaf senescence, and enhanced antioxidant capacity compared to wild-type plants under salt stress conditions.
Article
Plant Sciences
Marcio Alves-Ferreira, Frank Wellmer, Aline Banhara, Vijaya Kumar, Jose Luis Riechmann, Elliot M. Meyerowitz
Article
Biochemistry & Molecular Biology
Rubini Kannangara, Caroline Branigan, Yan Liu, Teresa Penfield, Vijaya Rao, Gregory Mouille, Herman Hofte, Markus Pauly, Jose Luis Riechmann, Pierre Broun