Article
Biochemistry & Molecular Biology
Siyu Liu, Judith Mine-Hattab, Marie Villemeur, Raphael Guerois, Henrik Dahl Pinholt, Leonid A. Mirny, Angela Taddei
Summary: Homologous recombination (HR) is a major pathway for DNA double-strand break repair. Rad51-single-stranded DNA nucleoprotein filaments (NPFs) play a crucial role in the homology search process. In this study, the authors develop and characterize a functional tagged version of Rad51, allowing for dynamic, in vivo studies of NPF formation. They observe that the NPFs exhibit flexibility and adopt various shapes in vivo, shedding new light on the function of Rad54 and Srs2. The formation of extended filaments and their compaction-extension dynamics provide an efficient search strategy for homologous sequences.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Wei Lee, Hiroshi Iwasaki, Hideo Tsubouchi, Hung-Wen Li
Summary: In meiosis, Dmc1 and Rad51 are responsible for pairing and exchanging strands of homologous chromosomes. The mechanisms by which Swi5-Sfr1 and Hop2-Mnd1 stimulate Dmc1-driven recombination are still unclear. Through smFRET and TPM experiments, it was found that both Hop2-Mnd1 and Swi5-Sfr1 individually enhance Dmc1 filament assembly on ssDNA, and when added together, they provide further stimulation. The binding rate of Dmc1 is enhanced by Hop2-Mnd1 while the dissociation rate is specifically reduced by Swi5-Sfr1 during nucleation.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Jakub Muraszko, Karol Kramarz, Bilge Argunhan, Kentaro Ito, Gabriela Baranowska, Yumiko Kurokawa, Yasuto Murayama, Hideo Tsubouchi, Sarah Lambert, Hiroshi Iwasaki, Dorota Dziadkowiec
Summary: Rrp1 plays a role in modulating Rad51 function by alleviating toxicity associated with excessive Rad51 levels through its ATPase domain. It interacts directly with Rad51, removes it from double-stranded DNA, and has E3 ubiquitin ligase activity with Rad51 as a substrate, suggesting a multi-tiered regulation of Rad51 by Rrp1.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Luke A. Greenhough, Chih-Chao Liang, Ondrej Belan, Simone Kunzelmann, Sarah Maslen, Monica C. Rodrigo-Brenni, Roopesh Anand, Mark Skehel, Simon J. Boulton, Stephen C. West
Summary: Homologous recombination is a vital process in life, involved in replication fork protection, DNA repair, and genetic exchange. Mutations in key recombination genes increase the risk of various cancers and Fanconi anemia. The RAD51 paralogues, including RAD51B, RAD51C, RAD51D, and XRCC2, play important roles in these processes, as demonstrated by their structure and function in the RAD51B-RAD51C-RAD51D-XRCC2 complex (BCDX2). BCDX2 stimulates RAD51 filament formation for recombinational DNA repair, crucial for tumor avoidance.
Article
Multidisciplinary Sciences
Shih-Chi Luo, Hsin-Yi Yeh, Wei-Hsuan Lan, Yi-Min Wu, Cheng-Han Yang, Hao-Yen Chang, Guan-Chin Su, Chia-Yi Lee, Wen-Jin Wu, Hung-Wen Li, Meng-Chiao Ho, Peter Chi, Ming-Daw Tsai
Summary: RAD51 and DMC1 recombinases catalyse high-fidelity and mismatch tolerant recombination, processes that are indispensable for the maintenance of genomic integrity. The study elucidates the structural difference between RAD51 and DMC1 with regard to mismatch tolerance, showing that key residues in Loop 1 and Loop 2 play a crucial role in determining the fidelity of DNA recombinases.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Tohru Minamino, Yusuke Morimoto, Miki Kinoshita, Keiichi Namba
Summary: The flagellar export chaperones, including FlgN, FliS, and FliT, play crucial roles in facilitating the efficient export of essential component proteins for filament formation. Dynamic interactions between these chaperones and the transmembrane export gate protein FlhA are believed to determine the order of substrate export.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hamida Laroussi, Ariadna B. Juarez-Martinez, Aline Le Roy, Elisabetta Boeri Erba, Frank Gabel, Bernard de Massy, Jan Kadlec
Summary: Meiotic recombination in mice is initiated by DNA double-strand breaks (DSBs), catalyzed by the TOPOVIL complex. This complex's activity is regulated by meiotic factors such as REC114, MEI4, and IHO1, but the mechanism is not well understood. This study reveals the molecular details of these factors and their interactions, including REC114 forming homodimers, associating with MEI4 as a heterotrimer, and IHO1 forming tetramers. The study also shows that IHO1 directly interacts with REC114 and shares a binding surface with TOPOVIBL and another meiotic factor ANKRD31. These findings suggest the existence of a ternary IHO1-REC114-MEI4 complex and highlight the potential regulatory role of REC114 in mediating interactions with multiple partners.
Article
Food Science & Technology
Yujie Song, Ting Li, Xinxia Zhang, Li Wang
Summary: Protein fibrillation is influenced by NaCl concentrations, with lower concentrations leading to positively charged aggregates and higher concentrations resulting in amorphous aggregates. Changes in secondary structure and aggregation morphology of rice proteins were observed through CD and AFM analysis under different NaCl concentrations.
Article
Cell Biology
Ying Chen, Haoyue Qi, Lijia Yang, Liang Xu, Jiaxuan Wang, Jiazhuo Guo, Liang Zhang, Yuanyuan Tan, Ronghui Pan, Qingyao Shu, Qian Qian, Shiyong Song
Summary: In this study, a loss-of-function mutant of OsbHLH002/OsICE1, a bHLH transcription factor, exhibited a lodging phenotype. It was found that OsbHLH002 and OSH1 interacted and shared common targets, and their binding capacity on OsMYB61, a key regulatory factor in secondary cell wall (SCW) development, was regulated by SLENDER RICE1, rice ortholog of KNOTTED ARABIDOPSIS THALIANA7, and OsNAC31.
Article
Biochemistry & Molecular Biology
Jingfei Xu, Lingyun Zhao, Sijia Peng, Huiying Chu, Rui Liang, Meng Tian, Philip P. Connell, Guohui Li, Chunlai Chen, Hong-Wei Wang
Summary: The study revealed major conformational differences between Rad51 and Dmc1 in their Loop2 regions, which play crucial roles in homologous recombination (HR). Key residues in the Loop2 region of human RAD51 and DMC1 were identified to regulate mismatch tolerance during strand exchange in HR, providing mechanistic insights into the specific roles of these proteins in DNA double-strand break repair.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Yuying Zhao, Ning Liu, Piaoxue Liu, Taojian Fan, Rui Ma, Huijie Hong, Xiaojia Chen, Zhongjian Xie, Han Zhang, Qi Wang, Tongkai Chen
Summary: This study presents a drug delivery system using PEGylated two-dimensional boron nanosheets loaded with gambogic acid for breast cancer treatment, allowing both multimodal imaging and photothermal conversion while inhibiting heat shock protein 90. This combination offers improved anti-tumor effects, reduced toxicity of photothermal therapy, inhibition of tumor growth, and new potential for combining chemotherapy and photothermal therapy.
ASIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Review
Plant Sciences
Yihao Li, Xin Zhang, Yi Zhang, Haiyun Ren
Summary: This review focuses on the organization and dynamics of actin filaments and microtubule network in guard cells during stomatal movement. The role of cytoskeletal-associated proteins in cytoskeletal rearrangements during stomatal movement is also discussed. The potential mechanisms of stomatal movement in relation to the cytoskeleton are explored, providing a foundation for further research in this field.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Yan Huo, Jing Zhao, Xiangyi Meng, Jie Yang, Ziyu Zhang, Zhiwei Liu, Rongxiang Fang, Lili Zhang
Summary: This study elucidated the function of an insect saliva protein in mediating insect feeding and arbovirus transmission by sequencing the saliva proteins of Laodelphax striatellus. The saliva protein was found to be involved in the formation of the salivary sheath, which is crucial for sap feeding and viral transmission.
PEST MANAGEMENT SCIENCE
(2022)
Article
Nanoscience & Nanotechnology
Moumita Ghosh, Anna Majkowska, Rajkumar Mirsa, Santu Bera, Jose Carlos Rodriguez-Cabello, Alvaro Mata, Lihi Adler-Abramovich
Summary: This study demonstrates the temperature-independent formation of stable membranes by the spontaneous interaction of intrinsically disordered elastin-like polypeptides (ELPs) with short aromatic peptides, offering novel possibilities for controlling the conformational restriction of intrinsically disordered proteins and using them in the design of new materials. The membranes are stable over time and display durability over a wide range of environmental parameters, supporting preosteoblast cell adhesion and proliferation as well as pH-dependent cargo release. Simple noncovalent interactions with short aromatic peptides can overcome conformational restrictions due to the phase transition to facilitate the formation of complex bioactive scaffolds.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Multidisciplinary Sciences
L. Robert Hollingsworth, Liron David, Yang Li, Andrew R. Griswold, Jianbin Ruan, Humayun Sharif, Pietro Fontana, Elizabeth L. Orth-He, Tian-Min Fu, Daniel A. Bachovchin, Hao Wu
Summary: NLRP1 and CARD8 form supramolecular signaling complexes called canonical inflammasomes upon activation, leading to caspase-1 activation, cytokine maturation, and/or pyroptotic cell death. Their C-terminal fragments containing a CARD and the UPA subdomain facilitate self-oligomerization, recruiting the inflammasome adaptor ASC or caspase-1 for further signaling.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Masako Koyama, Hitoshi Kurumizaka
JOURNAL OF BIOCHEMISTRY
(2018)
Article
Andrology
K. Shiraishi, A. Shindo, A. Harada, H. Kurumizaka, H. Kimura, Y. Ohkawa, H. Matsuyama
Article
Biochemistry & Molecular Biology
Di Luo, Daiki Kato, Jumpei Nogami, Yasuyuki Ohkawa, Hitoshi Kurumizaka, Hidetoshi Kono
NUCLEIC ACIDS RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Akihisa Osakabe, Zdravko J. Lorkovic, Wataru Kobayashi, Hiroaki Tachiwana, Ramesh Yelagandula, Hitoshi Kurumizaka, Frederic Berger
NUCLEIC ACIDS RESEARCH
(2018)
Article
Multidisciplinary Sciences
Akihito Harada, Kazumitsu Maehara, Yusuke Ono, Hiroyuki Taguchi, Kiyoshi Yoshioka, Yasuo Kitajima, Yan Xie, Yuko Sato, Takeshi Iwasaki, Jumpei Nogami, Seiji Okada, Tetsuro Komatsu, Yuichiro Semba, Tatsuya Takemoto, Hiroshi Kimura, Hitoshi Kurumizaka, Yasuyuki Ohkawa
NATURE COMMUNICATIONS
(2018)
Article
Biochemistry & Molecular Biology
Yoshimasa Takizawa, Hiroki Tanaka, Shinichi Machida, Masako Koyama, Kazumitsu Maehara, Yasuyuki Ohkawa, Paul A. Wade, Matthias Wolf, Hitoshi Kurumizaka
Article
Biochemical Research Methods
Yasuhiro Arimura, Tomonori Kono, Kuniki Kino, Hitoshi Kurumizaka
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2018)
Article
Multidisciplinary Sciences
Kana Tanabe, Jiaan Liu, Daiki Kato, Hitoshi Kurumizaka, Kenzo Yamatsugu, Motomu Kanai, Shigehiro A. Kawashima
SCIENTIFIC REPORTS
(2018)
Correction
Biochemistry & Molecular Biology
Wakana Iwasaki, Yuta Miya, Naoki Horikoshi, Akihisa Osakabe, Hiroyuki Taguchi, Hiroaki Tachiwana, Takehiko Shibata, Wataru Kagawa, Hitoshi Kurumizaka
Article
Cell Biology
Akihito Harada, Kazumitsu Maehara, Tetsuya Handa, Yasuhiro Arimura, Jumpei Nogami, Yoko Hayashi-Takanaka, Katsuhiko Shirahige, Hitoshi Kurumizaka, Hiroshi Kimura, Yasuyuki Ohkawa
NATURE CELL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Tomoya Kujirai, Haruhiko Ehara, Yuka Fujino, Mikako Shirouzu, Shun-ichi Sekine, Hitoshi Kurumizaka
Article
Multidisciplinary Sciences
Lot Snijders Blok, Justine Rousseau, Joanna Twist, Sophie Ehresmann, Motoki Takaku, Hanka Venselaar, Lance H. Rodan, Catherine B. Nowak, Jessica Douglas, Kathryn J. Swoboda, Marcie A. Steeves, Inderneel Sahai, Connie T. R. M. Stumpel, Alexander P. A. Stegmann, Patricia Wheeler, Marcia Willing, Elise Fiala, Aaina Kochhar, William T. Gibson, Ana S. A. Cohen, Ruky Agbahovbe, A. Micheil Innes, P. Y. Billie Au, Julia Rankin, Ilse J. Anderson, Steven A. Skinner, Raymond J. Louie, Hannah E. Warren, Alexandra Afenjar, Boris Keren, Caroline Nava, Julien Buratti, Arnaud Isapof, Diana Rodriguez, Raymond Lewandowski, Jennifer Propst, Ton van Essen, Murim Choi, Sangmoon Lee, Jong H. Chae, Susan Price, Rhonda E. Schnur, Ganka Douglas, Ingrid M. Wentzensen, Christiane Zweier, Andre Reis, Martin G. Bialer, Christine Moore, Marije Koopmans, Eva H. Brilstra, Glen R. Monroe, Koen L. van Gassen, Ellen van Binsbergen, Ruth Newbury-Ecob, Lucy Bownass, Ingrid Bader, Johannes A. Mayr, Saskia B. Wortmann, Kathy J. Jakielski, Edythe A. Strand, Katja Kloth, Tatjana Bierhals, John D. Roberts, Robert M. Petrovich, Shinichi Machida, Hitoshi Kurumizaka, Stefan Lelieveld, Rolph Pfundt, Sandra Jansen, Pelagia Deriziotis, Laurence Faivre, Julien Thevenon, Mirna Assoum, Lawrence Shriberg, Tjitske Kleefstra, Han G. Brunner, Paul A. Wade, Simon E. Fisher, Philippe M. Campeau
NATURE COMMUNICATIONS
(2018)
Article
Multidisciplinary Sciences
Tomoya Kujirai, Christian Zierhut, Yoshimasa Takizawa, Ryan Kim, Lumi Negishi, Nobuki Uruma, Seiya Hirai, Hironori Funabiki, Hitoshi Kurumizaka
Review
Cell Biology
Tomoya Kujirai, Haruhiko Ehara, Shun-ichi Sekine, Hitoshi Kurumizaka
Summary: In eukaryotes, genomic DNA is packaged into chromatin and the nucleosome acts as a barrier to transcription. The RNA polymerase II elongation complex disassembles the nucleosome during transcription to allow passage. After transcription, the nucleosome is rebuilt by transcription-coupled nucleosome reassembly, which plays a crucial role in preserving epigenetic information. The histone chaperone FACT is involved in nucleosome disassembly, maintenance, and reassembly during transcription in chromatin. Recent structural studies have provided insights into the structural transitions of the nucleosome during transcription.
Article
Multidisciplinary Sciences
Masahiro Nishimura, Yoshimasa Takizawa, Kayo Nozawa, Hitoshi Kurumizaka
Summary: The study reveals the structure of p53 binding to a nucleosome, providing valuable insights into the mechanism by which p53 binds to its target DNA and changes the chromatin structure for gene activation.