期刊
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS
卷 268, 期 10, 页码 1671-1675出版社
ELSEVIER
DOI: 10.1016/j.nimb.2010.02.128
关键词
Ion Beam Analysis; PIXE; PESA; Metalloprotein
类别
资金
- National Science Foundation [MRI-0319523, MRI-0116264, RUI:PHY-0354920, RUI:PHY-0651627]
An absolute measurement of the heavy element concentrations in a dried sample of aqueous protein solution has been combined with the absolute measurement of protein molecular concentration in the same sample. The ratio of these two measurements yields the metal-to-protein compositional stoichiometry of the metalloprotein. This combination of two Ion Beam Analysis techniques (Particle-Induced X-ray Emission and Proton Elastic Scattering Analysis) allows quantitative assessment of the metal atom to protein ratio in metalloproteins without direct measurement of sulfur atoms within the protein for the first time. While these results only demonstrate success with a single well-known metalloprotein, this combination of measurement ratios holds promise for future Ion Beam Analysis studies of metalloproteins. (C) 2010 Elsevier B.V. All rights reserved.
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