期刊
NMR IN BIOMEDICINE
卷 25, 期 3, 页码 464-475出版社
WILEY
DOI: 10.1002/nbm.1649
关键词
poly(D,L)-lactic-co-glycolide; magic angle spinning; order parameter; bone apatite; bone collagen
资金
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation [SFB 67, A1, A6, B5]
A combination of solid-state NMR spectroscopy and MRI was used to evaluate the formation of extracellular matrix in poly(D,L-lactide-co-glycolide) (PLGA) bone implants. Porous PLGA scaffolds were implanted into rat tibiae and analysed after 2, 4 or 8 weeks. MRI clearly delineated the implants within the cancellous bone. Differences in the trabecular structure of the implanted material and native bone were demonstrated. In addition, implants were analyzed by solid-state NMR spectroscopy under magic angle spinning. 13C NMR spectra showed the unambiguous signature of collagen formed in the scaffolds, but also the characteristic signals of the PLGA matrix, indicating that resorption was not complete after 8 weeks. Furthermore, 31P NMR spectroscopy detected the inorganic component of the matrix, which is composed of bioapatite. 31P NMR spectra were quantified and this analysis revealed that the amount of inorganic extracellular matrix formed de novo was significantly lower than in native bone. This demonstrates that solid-state NMR spectroscopy, in particular in combination with MRI, can provide useful information on the composition and structure of the extracellular matrix, and serve as a tool to evaluate the quality of tissue engineering strategies. Copyright (c) 2011 John Wiley & Sons, Ltd.
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