Article
Biochemistry & Molecular Biology
Tao Wang, Chuqiao Yuan, Jia Liu, Liangyan Deng, Wei Li, Junling He, Honglin Liu, Liping Qu, Jianming Wu, Wenjun Zou
Summary: This study aims to explore the energy protection effect of DXXK on cardiotoxicity induced by DOX. The results showed that DXXK can treat DOX-induced cardiotoxicity through the AMPK-mediated energy protection pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Limei Chen, Hui Wang, Zhao Chen, Wenhao Zhuo, Ruixiang Xu, Xin Zeng, Qirui He, Yongmei Guan, Hui Li, Hongning Liu
Summary: Dried ginger has a gastroprotective effect and can increase the activity of Ca2+-Mg2+-ATPase, SDH, ATP levels, and mitochondrial metabolic rate in stomach tissue of rats. These effects might be related to various metabolic pathways.
CHEMISTRY & BIODIVERSITY
(2022)
Article
Endocrinology & Metabolism
Min Chen, Qi-da He, Jing-jing Guo, Qi-biao Wu, Qi Zhang, Yuen-ming Yau, Yu-feng Xie, Zi-yi Guo, Zi-yan Tong, Zong-bao Yang, Lu Xiao
Summary: This study investigated the effect of electro-acupuncture (EA) on the liver and kidney metabolites in mice with premature ovarian failure (POF) through metabolomics. The results showed that EA improved ovarian function and regulated energy and neurotransmitter metabolism in the liver and kidney of POF mice, suggesting that EA therapy may be related to POF in traditional Chinese medicine (TCM) based on the results for the liver and kidney.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Multidisciplinary Sciences
Paulina Wigner, Krzysztof Zielinski, Magdalena Labieniec-Watala, Agnieszka Marczak, Marzena Szwed
Summary: The DOX-Tf conjugate as a drug delivery system exhibits higher cytotoxicity in human breast adenocarcinoma cells, especially in triple-negative breast cancer cells. Our findings also suggest that the functionality of cellular antioxidant systems and expression levels of estrogen receptors in breast cancer cells may influence the sensitivity to DOX-Tf conjugate.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Wanting Chen, Qian Li, Ranran Hou, Huaguo Liang, Yongli Zhang, Yongxia Yang
Summary: This study found that taurine has inhibitory effects on the growth and lung metastasis of breast cancer, and improves the pathological structure and metabolic disorders of tumor and lung tissue. Taurine also reverses the changes in various metabolites caused by breast cancer progression. The results suggest that taurine exerts anti-breast cancer effects through the regulation of metabolic pathways.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Biochemical Research Methods
Francesca Di Cesare, Matteo Calgaro, Veronica Ghini, Diletta Francesca Squarzanti, Annachiara De Prisco, Annalisa Visciglia, Paola Zanetta, Roberta Rolla, Paola Savoia, Angela Amoruso, Barbara Azzimonti, Nicola Vitulo, Leonardo Tenori, Claudio Luchinat, Marco Pane
Summary: This study investigated the effects of probiotic treatment on the metabolic profiles of human urine and serum using NMR-based metabonomic approach. The results showed significant impacts of probiotics on urinary and serum metabolomes, without altering their phenotypes. The study demonstrated the variations of metabolite levels resulting from different probiotic posologies.
JOURNAL OF PROTEOME RESEARCH
(2023)
Review
Food Science & Technology
Anees Ahmed Khalil, Md. Mominur Rahman, Abdur Rauf, Md. Rezaul Islam, Sultana Juhara Manna, Ammar Ahmed Khan, Samee Ullah, Muhammad Nadeem Akhtar, Abdullah S. M. Aljohani, Waleed Abdulmonem, Jesus Simal-Gandara
Summary: The olive family, particularly Olea europaea L., is widely used for olive oil production. Different parts of the olive tree contain bioactive compounds such as oleuropein, apigenin, hydroxytyrosol, etc. Researchers have utilized various extraction methods to increase oleuropein yield, including maceration, soxhlet, microwave-assisted, ultrasonication, and supercritical fluid methods. This review focuses on in vitro and in vivo studies exploring the nutraceutical properties of oleuropein, revealing its potential in treating obesity, diabetes, cardiovascular complications, neurodegenerative diseases, cancer, inflammation, microbial infections, and oxidation. The comprehensive literature provided in this review contributes to the scientific community's understanding of oleuropein's absorption, metabolism, bioavailability, extraction techniques, and nutraceutical perspectives.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Endocrinology & Metabolism
Yunfang Jia, Xianming Hou, Xiaomeng Lang, Yali Wang
Summary: In this study, the mechanism of Shugan Jianpi Qingre Fang in the treatment of functional dyspepsia was explored using UHPLC-QTOFMS. The results showed that after the intervention of Shugan Jianpi Qingre Fang, the levels of choline, proline, taurine, glutamic acid, and mannose in the plasma of the model group significantly regressed. By regulating glucose metabolism, energy metabolism, affecting nerve signal transduction and enhancing antioxidant capacity, Shugan Jianpi Qingre Fang plays an important role in the treatment of functional dyspepsia.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2023)
Article
Biochemistry & Molecular Biology
Yu Zhou, Xi Liu, Caihua Huang, Donghai Lin
Summary: Lactate, a compound fuel in metabolism, remains unclear whether it plays an important role in muscle cell metabolism. This study assessed the effects of lactate on myoblasts and clarified the underlying metabolic mechanisms. The results showed that lactate treatment promoted the proliferation and differentiation of myoblasts, and regulated metabolisms by promoting lactate intake and utilization, activating the TCA cycle, and increasing energy production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Qian Li, Wanting Chen, Wenbin Huang, Ranran Hou, Xinping Huang, Man Xu, Limei Que, Lijing Wang, Yongxia Yang
Summary: This study investigated the changes in serum metabolism in ApoE(-/-);SAP(-/-) mice compared to ApoE(-/-) mice during the progression of atherosclerosis using H-1-NMR metabonomics. The results showed that SAP deficiency regulated the levels of acetate, pyruvate, choline, and VLDL + LDL, which are metabolic markers, and this regulation occurred prior to the appearance of atherosclerotic plaques.
Article
Plant Sciences
Fei Mu, Rui Lin, Xueyan Lu, Meina Zhao, Jiaxin Zhao, Shaojie Huang, Chao Guo, Yue Guan, Haiyue Zhang, Miaomiao Xi, Jingwen Wang, Haifeng Tang
Summary: This study aimed to elucidate the protective effects of styrax on ischemic stroke and its underlying mechanisms. The results showed that styrax could improve the symptoms of ischemic stroke rats by intervening with energy metabolism and glutamine metabolism. This finding helps us better understand the mechanism of styrax and promote its clinical application.
PHARMACEUTICAL BIOLOGY
(2023)
Article
Veterinary Sciences
Zhiwei Kong, Bin Li, Chuanshe Zhou, Qinghua He, Yuzhong Zheng, Zhiliang Tan
Summary: This experiment revealed the mechanism of hypoxia adaptation, showing that hypoxia exposure is associated with elevated glycerophospholipid metabolism mediated by AGPAT2, leading to lipid disorders and apoptosis. These findings update the understanding of increased adaptability of dairy cows exposed to hypoxia at the metabolic level.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Tom Kretzschmar, Jasmine M. F. Wu, P. Christian Schulze
Summary: Heart failure remains the most common cause of death in the industrialized world, and despite new therapeutic interventions, complete prevention of its development and progression is still not possible, highlighting the need for further research to understand the underlying mechanisms. This review provides a detailed overview of the contribution of impaired mitochondrial dynamics and energy homeostasis during heart failure progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Organic
Zhenhui Wang, Xiaofeng Li, Wei Li, Yongyong Cao, Huaifeng Li
Summary: This study introduces a metal-free reductive acyldifluoroalkylation method for synthesizing β-difluoroalkyl ketones, which can proceed at room temperature. This method overcomes the limitations of previous synthesis methods that required heating or precious metal photocatalysts/oxidants, and it exhibits mild conditions, a broad substrate scope, and late-stage functionalization, facilitated by cooperative NHC and organophotocatalysis.
ORGANIC CHEMISTRY FRONTIERS
(2023)
Article
Medicine, General & Internal
Ljubica Tasic, Natasa Avramovic, Melissa Quintero, Danijela Stanisic, Lucas G. Martins, Tassia Brena Barroso Carneiro da Costa, Milka Jadranin, Maria Theresa de Souza Accioly, Paulo Faria, Beatriz de Camargo, Bruna M. de Sa Pereira, Mariana Maschietto
Summary: Pediatric cancer NMR-metabonomics offers a powerful tool for studying tumor development and improving cancer diagnosis and treatment. This study used HR-MAS NMR to analyze Wilms tumor and normal kidney samples, revealing significant differences in metabolites between the tumor and normal tissues. These findings provide insight into the altered metabolic pathways in Wilms tumor.
Article
Biochemistry & Molecular Biology
George Lambrinidis, Cedric Gouedard, Sotiria Stasinopoulou, Angeliki Angelopoulou, Vassiliki Ganou, Aggeliki K. Meligova, Dimitra J. Mitsiou, Panagiotis Marakos, Nicole Pouli, Emmanuel Mikros, Michael N. Alexis
Summary: Raloxifene analogues bearing a bulky basic side chain amino group showed higher ER antagonism in post-menopausal endometrium than raloxifene itself, particularly compound 13b. Compound 13b demonstrated potential for providing safer endometrial treatment for menopausal syndrome.
BIOORGANIC CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Anezia Kourkoulou, Iliana Zantza, Konstantina Foti, Emmanuel Mikros, George Diallinas
Summary: Members of the NAT family are symporters specific for the uptake of purines, pyrimidines, or L-ascorbic acid. This study reveals the roles of partially conserved residues in determining the specificity of the UapA transporter. Results show that UapA specificity is genetically modifiable, allowing for speculation on the flexible mechanism of transport.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biology
Stamatis Mourtakos, Anastassios Philippou, Anastasios Papageorgiou, Peter Lembessis, Stella Zaharinova, Yozlyam Hasanova, Rumiana Koynova, Fragiskos Bersimis, Boris Tenchov, Nikolaos Geladas, Emmanuel Mikros, Lampros S. Sidossis, Michael Koutsilieris
Summary: Analyzing the plasma heat capacity profile of albumin and globulins during extreme physical exercise using DSC revealed that prolonged intense exercise enhanced the thermal stability of plasma albumin, shifting its denaturation transition to higher temperatures. However, these alterations were not persistent, as virtually full recovery of the initial status was observed after a 30-day recovery period.
JOURNAL OF THERMAL BIOLOGY
(2021)
Article
Chemistry, Medicinal
Marina Bantzi, Fiona Augsburger, Jeremie Loup, Yan Berset, Sofia Vasilakaki, Vassilios Myrianthopoulos, Emmanuel Mikros, Csaba Szabo, Christian G. Bochet
Summary: A study identified a novel 3-MST inhibitor HMPSNE with micromolar potency, along with derivatives, showing inhibitory effects on colon cancer cell proliferation.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Stavros Beteinakis, Anastasia Papachristodoulou, Emmanuel Mikros, Maria Halabalaki
Summary: NMR-based metabolic profiling is widely used in food and plant sciences, but appropriate pre-processing procedures greatly affect the obtained metabolic profile. This study provides a detailed guide for untargeted NMR-based metabolic profiling of table olives, showing that pre-processing and sample preparation stages have a significant impact on the metabolic profile and spectral quality.
PHYTOCHEMICAL ANALYSIS
(2022)
Article
Behavioral Sciences
Aikaterini Iliou, Angeliki-Maria Vlaikou, Markus Nussbaumer, Dimitra Benaki, Emmanuel Mikros, Evangelos Gikas, Michaela D. Filiou
Summary: This study investigated the impact of acute stress on mouse cerebellar metabolomics, revealing altered levels of 19 out of 47 annotated metabolites. These metabolites are implicated in neurotransmission, NAA turnover, energy, and purine/pyrimidine metabolism. Additionally, correlations between FST behavioral parameters and levels of 4 metabolites were reported, highlighting a previously unexplored involvement of cerebellum in stress responses.
STRESS-THE INTERNATIONAL JOURNAL ON THE BIOLOGY OF STRESS
(2021)
Article
Biochemistry & Molecular Biology
Georgia Ntasi, Anthony Tsarbopoulos, Emmanuel Mikros, Evagelos Gikas
Summary: The article presents a new method for measuring nine metabolites in human blood plasma, which has been validated by assessing blood donor samples and demonstrating agreement with established baseline levels, proving the method's applicability.
Article
Biochemistry & Molecular Biology
Ekaterini Tatsaki, Eleni Anagnostopoulou, Iliana Zantza, Panayiota Lazou, Emmanuel Mikros, Stathis Frillingos
Summary: The study explores the relationship between sequence and specificity in membrane transporters using an evolutionary approach, revealing new specificity determinants in a closely related group of transporters. By reconstructing the ancestral protein and conducting mutagenesis experiments, the researchers identify five specific residues linked to changes in substrate specificity. These findings contribute to a better understanding of the molecular mechanisms underlying specificity in membrane transporters.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Cell Biology
Ioanna Dagla, Aikaterini Iliou, Dimitra Benaki, Evagelos Gikas, Emmanuel Mikros, Tina Bagratuni, Efstathios Kastritis, Meletios A. Dimopoulos, Evangelos Terpos, Anthony Tsarbopoulos
Summary: Vaccination is highly effective in mitigating the COVID-19 pandemic. However, individual immune responses to mRNA vaccines vary significantly. Metabolomics analysis reveals distinct plasma metabolic profiles associated with immune response levels, indicating the potential of certain metabolites as predictive markers for vaccine response.
Article
Chemistry, Medicinal
Maria A. Theodoropoulou, Giorgos S. Koutoulogenis, Linlin Zhang, Ifigeneia Akrani, Emmanuel Mikros, Rolf Hilgenfeld, George Kokotos
Summary: The development of novel agents to combat COVID-19 is crucial. This study focused on identifying an inhibitor that can simultaneously target both SARS-CoV-2 M-pro and PLA(2). Evaluation of various PLA(2) inhibitors revealed that the known potent inhibitor GK241 also weakly inhibits SARS-CoV-2 M-pro. Molecular docking and dynamics calculations provided insights into the interaction between GK241 and SARS-CoV-2 M-pro. The structure of 2-Oxoamide GK241 may serve as a lead for the development of dual PLA(2) and SARS-CoV-2 M-pro inhibitors.
Article
Biochemistry & Molecular Biology
Karim Zuhra, Maria Petrosino, Barkha Gupta, Theodora Panagaki, Marco Cecconi, Vassilios Myrianthopoulos, Roger Schneiter, Emmanuel Mikros, Tomas Majtan, Csaba Szabo
Summary: Epigallocatechin gallate (EGCG) is the main bioactive component of green tea and it inhibits cystathionine beta-synthase (CBS), a major H2S-generating enzyme. EGCG blocks a substrate access channel to pyridoxal-5'-phosphate and inhibits cellular H2S production in cancer cells and Down syndrome fibroblasts. This potent inhibition of CBS-derived H2S production may contribute to the pharmacological effects of EGCG in various pathophysiological conditions.
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Argyro Vontzalidou, Sapfo-Maria Dimitrakoudi, Konstantinos Tsoukalas, Grigoris Zoidis, Eliza Chaita, Evanthia Dina, Christina Cheimonidi, Ioannis P. Trougakos, George Lambrinidis, Alexios-Leandros Skaltsounis, Emmanuel Mikros, Nektarios Aligiannis
Summary: A number of stilbenoid and chalconoid derivatives were synthesized and evaluated for their ability to modulate tyrosinase activity and scavenge free radicals. The results showed that certain compounds exhibited potent tyrosinase inhibitory activity, while others enhanced the enzymatic activity. Moreover, some compounds demonstrated significant anti-melanogenic effects or melanogenic effects, as well as strong free radical scavenging activity.
Article
Biochemistry & Molecular Biology
Mohan Ghorasaini, Konstantina Ismini Tsezou, Aswin Verhoeven, Yassene Mohammed, Panayiotis Vlachoyiannopoulos, Emmanuel Mikros, Martin Giera
Summary: In this study, a workflow combining nuclear magnetic resonance (NMR) and mass spectrometry (MS) analysis was developed for lipid analysis in human plasma samples. The study found good agreement and correlation between the two platforms in lipid quantification and diagnostic performance. The research also provided insight into lipoprotein and lipid interactions and disease relevance.
Article
Food Science & Technology
Gabriela Belen Lemus Ringele, Stavros Beteinakis, Anastasia Papachristodoulou, Evangelos Axiotis, Emmanuel Mikros, Maria Halabalaki
Summary: Honey is a natural, healthy commodity and is considered one of the most complex foods produced by nature. NMR-based metabolite profiling combined with chemometrics was used in the quality control of Greek honeys, and STOCSY was employed for the first time as a tool for honey biomarker identification.
Meeting Abstract
Rheumatology
Konstantina Tsezou, Dimitra Benaki, Katerina Iliou, Emmanuel Mikros, Panayiotis Vlachoyiannopoulos
ARTHRITIS & RHEUMATOLOGY
(2021)