Article
Biochemistry & Molecular Biology
Sergey M. Korotkov, Artemy Novozhilov
Summary: This study found that the effects of thiol-modifying agents and MPTP inhibitors on MPTP opening were diverse and concentration-dependent. The research suggests that Tl+-induced MPTP opening in calcium-loaded mitochondrial inner membrane may be caused by the interaction of active groups. This study provides further insight into the causes of thallium toxicity and may be useful in developing new treatments for thallium poisoning.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yisang Yoon, Hakjoo Lee, Marilen Federico, Shey-Shing Sheu
Summary: Mitochondrial permeability transition (MPT) is a phenomenon that leads to mitochondrial dysfunction and cell injury. The opening of a mega-channel called the permeability transition pore (PTP) is responsible for MPT. Mitochondrial fission and fusion play a role in regulating MPT. There may also exist a non-conventional form of MPT that is insensitive to certain inhibitors.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2023)
Article
Biochemistry & Molecular Biology
Giuseppe Cannino, Andrea Urbani, Marco Gaspari, Mariaconcetta Varano, Alessandro Negro, Antonio Filippi, Francesco Ciscato, Ionica Masgras, Christoph Gerle, Elena Tibaldi, Anna Maria Brunati, Giorgio Colombo, Giovanna Lippe, Paolo Bernardi, Andrea Rasola
Summary: This study reveals that the mitochondrial chaperone TRAP1 interacts with F-ATP synthase, increasing its catalytic activity and counteracting the inhibitory effect of CyPD. Additionally, TRAP1 directly inhibits the channel activity of F-ATP synthase and reverses PTP induction by CyPD, thus preventing mitochondrial depolarization and cell death. These findings highlight the importance of TRAP1 in cell bioenergetics and survival.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Biochemistry & Molecular Biology
Sergey M. Korotkov, Artemy V. Novozhilov
Summary: Lysine residues are important in regulating enzymatic activity and maintaining the spatial structure of mitochondrial proteins. The study found that the Tl+-induced MPTP opening in rat liver mitochondria was influenced by the conformation of the adenine nucleotide translocase (ANT). The effects of lysine reagents (pyridoxal 5-phosphate) and thiol reagents on MPTP opening were investigated, and it was observed that increasing the concentration of these reagents led to more noticeable pore opening. The study suggests that the state of lysines forming the pore structure may contribute to the Tl+-induced MPTP opening.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Xavier R. Chapa-Dubocq, Keishla M. Rodriguez-Graciani, Nelson Escobales, Sabzali Javadov
Summary: Mitochondria, known as the powerhouse of the cell, regulate various cellular processes including ion homeostasis, energy production, and cell death. The inner mitochondrial membrane (IMM) plays a critical role in controlling mitochondrial metabolism and function. The volume of the mitochondrial matrix, regulated by ion transport mechanisms, influences IMM remodeling and can affect mitochondrial respiration and cell survival. Despite extensive research, the mechanisms underlying changes in matrix volume and IMM remodeling in response to energy and oxidative stressors remain poorly understood. This review summarizes previous studies and discusses the interplay between matrix volume regulation and IMM remodeling.
Review
Biochemistry & Molecular Biology
Hagai Rottenberg
Summary: It is widely reported that the mitochondrial membrane potential, increment ψm, is reduced in aging animals. It was recently suggested that the lower increment ψm in aged animals modulates mitochondrial bioenergetics and that this effect is a major cause of aging since artificially increased increment ψm in C. elegans increased lifespan. The activation of the voltage-gated mitochondrial permeability transition pore (mPTP) is the main cause of the increase in depolarized mitochondria in aged cells, inhibiting oxidative phosphorylation, releasing calcium and mROS, and depleting NAD(+), thereby accelerating degenerative diseases and aging. The reported lifespan extension by artificially generated increment ψm in C. elegans is best explained by inhibition of the voltage-gated mPTP.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Paulina Patalas-Krawczyk, Dominika Malinska, Jaroslaw Walczak, Gilles Kratzer, Monika Prill, Bernadeta Michalska, Karolina Drabik, Bjorn Titz, Yvan Eb-Levadoux, Thomas Schneider, Jedrzej Szymanski, Julia Hoeng, Manuel C. Peitsch, Jerzy Duszynski, Joanna Szczepanowska, Marco Van der Toorn, Carole Mathis, Mariusz R. Wieckowski
Summary: Mitochondria play an important role in responding to stress factors, and alkaloids from tobacco plants such as nicotine, anatabine, and anabasine can modulate mitochondrial activities differently in mouse brain and liver tissues. These alkaloids affect mitochondrial bioenergetics parameters, including oxygen consumption and swelling rates, with potential toxic effects observed in liver mitochondria. The observed effects are likely independent of nicotinic acetylcholine receptors based on quantitative proteomic analysis.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Polymer Science
Hong Gao, Yingchun Sun, Miaomiao Wang, Bo Wu, Guoqiang Han, Ling Jin, Kui Zhang, Youyi Xia
Summary: This article introduces a novel acrylate-based elastomer with exchangeable disulfide crosslinks for self-healing and reprocessing capabilities. The use of a base system as a catalyst proves to be highly efficient, enabling the material to quickly restore over 90% of its mechanical properties within 5 hours, showing promising application potential as recoverable adhesives.
Article
Pharmacology & Pharmacy
Vincenzo Algieri, Cristina Algieri, Paola Costanzo, Giulia Fiorani, Antonio Jiritano, Fabrizio Olivito, Matteo Antonio Tallarida, Fabiana Trombetti, Loredana Maiuolo, Antonio De Nino, Salvatore Nesci
Summary: An efficient, eco-compatible, and inexpensive method for constructing fully substituted pyrazoles (Pzs) was developed via the eliminative nitrilimine-alkene 1,3-dipolar cycloaddition (ENAC) reaction. The reaction yielded high regioselectivity and excellent yield, with enaminones and nitrilimines as dipolarophiles and dipoles, respectively. The F1FO-ATPase activity and mitochondrial permeability transition pore (mPTP) opening were evaluated for three different structures of Pzs, showing reduced activity and concentration-dependent inhibitory effects.
Article
Biochemistry & Molecular Biology
Giuseppe Federico Amodeo, Natalya Krilyuk, Evgeny Pavlov
Summary: The c subunit of ATP synthase is not only a component of the rotor, but also exhibits ion channel activity. The presence of CypD increases the conductance of the c subunit, potentially enhancing its role in mitochondrial toxicity. CypD is essential for the formation of c subunit-induced PTP, but may not be integral to the pore itself.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Giampaolo Morciano, Natalia Naumova, Piotr Koprowski, Sara Valente, Vilma A. Sardao, Yaiza Potes, Alessandro Rimessi, Mariusz R. Wieckowski, Paulo J. Oliveira
Summary: This review summarizes the intriguing phenomenon of the mitochondrial permeability transition pore (mPTP) in cell biology. Despite almost 50 years of research, the mechanisms of mPTP are still not definitively understood. From initially being considered an in vitro artifact to now being recognized for its physiological and pathological implications, the journey of mPTP research has been long and complex.
BIOLOGICAL REVIEWS
(2021)
Article
Cell Biology
Ji Geng, Tejinder Pal Khaket, Jie Pan, Wen Li, Yan Zhang, Yong Ping, Maria Inmaculada Cobos Sillero, Bingwei Lu
Summary: FMRP interacts with VDAC to regulate the formation and function of ERMCSs, which are critical for mito-Ca2+ homeostasis. Excessive ERMCS formation and ER-to-mitochondria Ca2+ transfer are observed in FXS. Inhibition of VDAC or other ERMCS components restores synaptic structure and function, rescues locomotion and cognitive deficits in FXS models.
DEVELOPMENTAL CELL
(2023)
Article
Environmental Sciences
Yingmin Liang, Pak Hin Chu, Linwei Tian, Kin Fai Ho, Mary Sau Man Ip, Judith Choi Wo Mak
Summary: This study found that PM2.5 leads to the opening of mitochondrial permeability transition pore (mPTP), resulting in cell apoptosis and mitochondrial dysfunction. The opening of mPTP is achieved through upregulation of VDAC channel and reduction in nuclear expression of PPARgamma and PGC-1 alpha. Furthermore, the use of mPTP targeting compounds can inhibit PM2.5-induced mPTP opening and cell apoptosis, and preserve mitochondrial function.
ENVIRONMENTAL POLLUTION
(2022)
Article
Cell Biology
Sarah K. Skinner, Angelo Solania, Dennis W. Wolan, Michael S. Cohen, Terence E. Ryan, Russell T. Hepple
Summary: The study found that mitochondrial permeability transition (MPT) is a novel mechanism of skeletal muscle atrophy, operating through the release of mROS and activation of caspase-3. The experiment demonstrated that inhibiting MPT, mROS, or caspase-3 could prevent muscle atrophy in both chemical-induced and disuse models.
Review
Immunology
Kathrin A. A. Bruecksken, Paola Palacio, Eva-Maria Hanschmann
Summary: Posttranslational modifications play a crucial role in controlling molecular and cellular functions. Thiol modifications of cysteine residues are important for inflammatory signaling, gene expression regulation, cytokine release, and antigen presentation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Julia Claudiane da Veiga, Neidiquele Maria Silveira, Amedea Barozzi Seabra, Ilana Urbano Bron
Summary: This review investigates the strategy of using nitric oxide (NO) donors to extend postharvest shelf-life and improve fruit quality, discusses the interactions between NO and ethylene or reactive oxygen species, and highlights the potential of nanotechnology in NO applications.
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
(2024)
