Article
Plant Sciences
Shuyun Chen, Tao Ma, Shiren Song, Xinlong Li, Peining Fu, Wei Wu, Jiaqi Liu, Yu Gao, Wenxiu Ye, Ian B. Dry, Jiang Lu
Summary: This study reveals that the oomycete pathogen HaRxLL470 effector protein enhances susceptibility of host plants to H. arabidopsidis by interacting with the host protein HY5 and compromising its function as a transcription factor, thereby suppressing the plant's immune response.
Article
Plant Sciences
Chao Li, Shaofeng Luo, Lu Feng, Qianqian Wang, Jiasen Cheng, Jiatao Xie, Yang Lin, Yanping Fu, Daohong Jiang, Tao Chen
Summary: This study reveals the important role of E3 ubiquitin ligases in the pathogenesis of Plasmodiophora brassicae, inhibiting host immunity by degrading host immune-associated cysteine proteases. The secreted E3 ubiquitin ligases from Plasmodiophora brassicae interact with and ubiquitinate host immune-associated cysteine protease RD21A, leading to compromised host immunity and increased susceptibility to the pathogen.
Article
Biology
Derek S. Lundberg, Pratchaya Pramoj Na Ayutthaya, Annett Strauss, Gautam Shirsekar, Wen-Sui Lo, Thomas Lahaye, Detlef Weigel
Summary: hamPCR is a robust strategy to quantify microbial load and describe interkingdom microbial community composition, providing an accessible experimental solution to the limitations and statistical challenges of compositional data in culture-independent microbiology.
Article
Plant Sciences
Peng Li, Wen Li, Xiaofan Zhou, Junjian Situ, Lizhu Xie, Pinggen Xi, Bo Yang, Guanghui Kong, Zide Jiang
Summary: In this study, a RXLR effector protein, PlAvh202, secreted by the destructive pathogen Peronophythora litchii was identified. PlAvh202 was found to suppress host ethylene production by targeting and destabilizing SAMS, leading to the suppression of plant immune responses and enhanced susceptibility to Phytophthora capsici.
Article
Plant Sciences
Zhiyuan Yin, Nan Wang, Weiwei Duan, Lei Pi, Danyu Shen, Daolong Dou
Summary: The CBM1 family is predominantly found in fungi and oomycetes, playing crucial roles in plant-microbe interactions. PcCBP3, identified in Phytophthora capsici, is an apoplastic effector that induces cell death in plants, suggesting its importance in the virulence of the pathogen.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Plant Sciences
Joon Yung Cha, Shahab Uddin, Donah Mary Macoy, Gyeong-Im Shin, Song Yi Jeong, Imdad Ali, Ji-Won Hwang, Myung Geun Ji, Sang Cheol Lee, Joung Hun Park, Marium Sultana, Gyeong Ryul Ryu, Gyeongik Ahn, Sang Yeol Lee, Min Gab Kim, Woe-Yeon Kim
Summary: Tomato production faces challenges from various biotic stresses, such as fungi, bacteria, and viruses. Researchers used the CRISPR/Cas9 system to modify tomato genes, resulting in increased resistance against bacterial and fungal pathogens. This study contributes to improving crop breeding for enhanced biotic stress resistance.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Xiaobin Ou, Tianqi Li, Yi Zhao, Yuankai Chang, Lihong Wu, Guoqingzi Chen, Brad Day, Kun Jiang
Summary: Mutants of the ABA signaling core compromised stomatal immunity, with a subset of calcium-dependent protein kinases (CPK4/5/6) playing a role in stomatal immune signaling. Additionally, CPKs regulate apoplastic ROS levels and reinforce the localized SA signal in guard cells, contributing to stomatal immunity.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Plant Sciences
Diego Rebaque, Irene del Hierro, Gemma Lopez, Laura Bacete, Francisco Vilaplana, Pietro Dallabernardina, Fabian Pfrengle, Lucia Jorda, Andrea Sanchez-Vallet, Rosa Perez, Frederic Brunner, Antonio Molina, Hugo Melida
Summary: The study found that MLGs are a group of carbohydrate-based molecular patterns perceived by plants, with MLG43 being the smallest structure capable of triggering strong pattern-triggered immunity responses and disease resistance. The mechanism of MLG43 recognition differs from that of other carbohydrate MAMPs, providing potential insights into plant immunity mechanisms.
Article
Plant Sciences
Genevieve Arsenault-Labrecque, Parthasarathy Santhanam, Yanick Asselin, Benjamin Cinget, Amandine Lebreton, Caroline Labbe, Francois Belzile, Mark Gijzen, Richard R. Belanger
Summary: In this study, a specific Avr gene interacting with the soybean resistance gene Rps8 was identified using genotyping-by-sequencing approach. The interaction between Rps8 and Avr3a was confirmed through functional analyses. This study also revealed a clear distinction between Rps3a and Rps8, which has not been previously reported.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Ziwei Wang, Tingting Li, Xiaojiang Zhang, Jiashu Feng, Zhuting Liu, Weixing Shan, Matthieu H. A. J. Joosten, Francine Govers, Yu Du
Summary: Ubiquitin-like domain-containing proteins (UDPs) are involved in the ubiquitin-proteasome system. The Phytophthora infestans effector Pi06432 targets the potato StUDP protein, leading to the suppression of salicylic acid (SA)-related immunity. StUDP interacts with and destabilizes the 26S proteasome subunit StRPT3b, repressing proteasome activity. This destabilization also decreases the stability of the master transcription factor SARD1 and lowers SA content.
Article
Multidisciplinary Sciences
Justine Bresson, Jasmin Doll, Francois Vasseur, Mark Stahl, Edda von Roepenack-Lahaye, Joachim Kilian, Bettina Stadelhofer, James M. Kremer, Dagmar Kolb, Stephan Wenkel, Ulrike Zentgraf
Summary: This study uncovers the genetic interaction between two key genes, WRKY53 and REVOLUTA, in drought-resistant plants, revealing how late and early developmental events are connected. The results also highlight the crucial roles of these genes in plant development and immunity.
Article
Nanoscience & Nanotechnology
Mohamed El-Shetehy, Aboubakr Moradi, Mattia Maceroni, Didier Reinhardt, Alke Petri-Fink, Barbara Rothen-Rutishauser, Felix Mauch, Fabienne Schwab
Summary: Silica nanoparticles have the potential to induce systemic acquired resistance in plants, entering leaves through stomata and involving the defense hormone salicylic acid. Compared to SiO2 NPs, the induction of systemic acquired resistance by Si(OH)(4) may cause stress.
NATURE NANOTECHNOLOGY
(2021)
Review
Plant Sciences
Chhana Ullah, Yen-Ho Chen, Maria A. Ortega, Chung-Ju Tsai
Summary: This review examines the differences in salicylic acid (SA) synthesis between Arabidopsis and poplar, focusing on the phenylalanine ammonia-lyase (PAL) pathway and the isochorismate synthase (ICS) pathway. It discusses potential factors influencing SA diversity in plant defense and suggests future research directions.
CURRENT OPINION IN PLANT BIOLOGY
(2023)
Article
Plant Sciences
Florian Dunker, Lorenz Oberkofler, Bernhard Lederer, Adriana Trutzenberg, Arne Weiberg
Summary: This study explored the gene function of Hyaloperonospora arabidopsidis using Arabidopsis host-induced gene silencing (HIGS) and ectopic expression in Nicotiana benthamiana. It found that HaCR1 played a crucial role in plant cell death and pathogen reproduction, affecting disease susceptibility in both biotrophic and necrotrophic pathogens. The research provides molecular evidence for the importance of this class of non-RxLR effectors in biotrophic oomycetes.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Julien Lang, Baptiste Genot, Jean Bigeard, Jean Colcombet
Summary: This study reveals that ETI-mediating NLRs and NLR signaling components NDR1 and EDS1 can promote the salicylic acid sector of defense downstream of MPK3 activity. It also demonstrates that both sustained and transient MPK3/6 activities positively control the expression of several NLR genes. The findings highlight the intricate interplay of PTI and ETI pathways.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Amey Redkar, Volkan Cevik, Kate Bailey, He Zhao, Dae Sung Kim, Zhou Zou, Oliver J. Furzer, Sebastian Fairhead, M. Hossein Borhan, Eric B. Holub, Jonathan D. G. Jones
Summary: The oomycete Albugo candida causes white blister rust, an important disease of Brassica crops. Different races of A. candida have different capacities to infect host plant species, which are associated with the polymorphic secreted proteins with a CX(2)CX(5)G ('CCG') motif. The resistance genes WRR4A and WRR4B in Arabidopsis thaliana confer resistance to multiple A. candida races and are triggered by specific CCG effector proteins, suggesting a potential mechanism for broad-spectrum resistance. This study provides insights into the interaction between Arabidopsis and A. candida.
Article
Entomology
Dae Sung Kim, Jiang Zhang
Summary: Sustainable agriculture relies on environmentally-friendly practices and technologies, and RNA interference (RNAi) has emerged as a promising approach for pest control. However, there are still several challenges to overcome in applying RNAi against a wide range of insect species.
ENTOMOLOGIA GENERALIS
(2023)
Article
Biochemistry & Molecular Biology
Gui Xiao, Wenjuan Wang, Muxing Liu, Ya Li, Jianbin Liu, Marina Franceschetti, Zhaofeng Yi, Xiaoyuan Zhu, Zhengguang Zhang, Guodong Lu, Mark J. Banfield, Jun Wu, Bo Zhou
Summary: The arms race between plants and pathogens has led to the development of sophisticated recognition mechanisms between host immune receptors and pathogen effectors. Different alleles of immune receptors in the host display distinct recognition against pathogen effectors. The Piks allele of the rice immune receptor Pik does not recognize any known AvrPik variants, unlike other Pik alleles. The amino acid residue Glu229 in Piks-1 is critical for breaking the canonical Pik/AvrPik recognition pattern.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Entomology
Yangcun Li, Hong Xu, Wanwan He, Haoling Rong, Shengchun Li, Dae Sung Kim, Peng Han, Yang Yang, Jiang Zhang
Summary: This study aimed to test whether silencing dsRNase could increase the RNAi effect in Colorado potato beetle (CPB) via plastid-mediated RNAi (PM-RNAi). The results showed that when the RNAi effect was weak or moderate, silencing dsRNase could improve the effect of PM-RNAi. This finding provides new evidence to enhance crop resistance to insect herbivores via an RNAi-of-RNAi approach.
ENTOMOLOGIA GENERALIS
(2023)
Article
Biochemistry & Molecular Biology
Yu Sugihara, Yoshiko Abe, Hiroki Takagi, Akira Abe, Motoki Shimizu, Kazue Ito, Eiko Kanzaki, Kaori Oikawa, Jiorgos Kourelis, Thorsten Langner, Joe Win, Aleksandra Bialas, Daniel Ludke, Mauricio P. Contreras, Izumi Chuma, Hiromasa Saitoh, Michie Kobayashi, Shuan Zheng, Yukio Tosa, Mark J. Banfield, Sophien Kamoun, Ryohei Terauchi, Koki Fujisaki
Summary: Studies integrating genetic analyses of rice and rice blast fungus have discovered a new pathogen recognition specificity in the immune receptor Pik, which mediates resistance to the fungus. The resistance is triggered by the recognition of a previously unidentified effector protein encoded by the avirulence gene AVR-Mgk1. This discovery highlights the complexity of host-pathogen gene-for-gene interactions and the coevolution between the immune receptor and effector proteins.
Article
Biochemistry & Molecular Biology
Jiashu Chu, Isabel Monte, Thomas A. DeFalco, Philipp Koster, Paul Derbyshire, Frank L. H. Menke, Cyril Zipfel
Summary: In this study, the researchers discovered that MpRBOH1 and MpPBLa, single members of the RBOH and PBL families in the liverwort Marchantia polymorpha, are essential for chitin-induced ROS production. MpPBLa directly interacts with and phosphorylates MpRBOH1, and this phosphorylation is crucial for chitin-induced ROS production mediated by MpRBOH1.
Editorial Material
Plant Sciences
Mark J. Banfield
Review
Plant Sciences
Shengchun Li, Dae Sung Kim, Jiang Zhang
Summary: RNA interference (RNAi) technology is a promising approach for pest control due to its high species selectivity. Recent advances in engineering plastid genome have shown that it is an effective way to protect plants from arthropod pests. This review discusses the progress, factors influencing efficacy, and strategies for improving efficiency of plastid-mediated RNAi (PM-RNAi) approach. The challenges and biosafety concerns of PM-RNAi technology for commercial production are also addressed.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Biology
Julia P. Vainonen, Richard Gossens, Julia Krasensky-Wrzaczek, Raffaella De Masi, Iulia Danciu, Tuomas Puukko, Natalia Battchikova, Claudia Jonak, Lennart Wirthmueller, Michael Wrzaczek, Alexey Shapiguzov, Jaakko Kangasjarvi
Summary: Transcriptional regulation in plants is controlled by RADICAL-INDUCED CELL DEATH1 (RCD1) and its inhibitory phosphorylation by photoregulatory protein kinases (PPKs). This study identifies RCD1 as a plant PAR-reader and reveals that PAR-binding determines RCD1 localization to nuclear bodies (NBs).
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hui Li, Jinlon Wang, Tung Ariel Kuan, Bozeng Tang, Li Feng, Jiuyu Wang, Zhi Cheng, Jan Sklenar, Michelle Hulin, Yufei Li, Yi Zhai, Yingnan Hou, Frank L. H. Menke, Yanli Wang, Wenbo Ma
Summary: Pathogens employ a strategy of molecular mimicry to hijack host phosphatase and diversify their effector repertoire by protein modularity. The (L)WY-LWY module in Phytophthora effectors recruits plant PP2A core enzyme to regulate host phosphoproteins. The conservation of the PP2A-interacting module but divergent C-terminal LWY units allows for functional diversity in effector-mediated manipulation of host cellular processes.
Review
Plant Sciences
Rafal Zdrzalek, Caroline Stone, Juan Carlos De la Concepcion, Mark J. Banfield, Adam R. Bentham
Summary: Factors such as climate change and increased global trade will worsen the prevalence of plant diseases, posing a significant threat to global food security. Developing new methods of pathogen control is crucial to effectively combat crop losses and protect plant health.
CURRENT OPINION IN PLANT BIOLOGY
(2023)
Article
Plant Sciences
Sanu Arora, Andrew Steed, Rachel Goddard, Kumar Gaurav, Tom O'Hara, Adam Schoen, Nidhi Rawat, Ahmed F. F. Elkot, Andrey V. V. Korolev, Catherine Chinoy, Martha H. H. Nicholson, Soichiro Asuke, Rea Antoniou-Kourounioti, Burkhard Steuernagel, Guotai Yu, Rajani Awal, Macarena Forner-Martinez, Luzie Wingen, Erin Baggs, Jonathan Clarke, Diane G. O. Saunders, Ksenia V. V. Krasileva, Yukio Tosa, Jonathan D. G. Jones, Vijay K. K. Tiwari, Brande B. H. Wulff, Paul Nicholson
Summary: Since 1985, wheat blast has spread throughout South America, Bangladesh, and Zambia. Two wheat resistance genes, Rwt3 and Rwt4, have been identified as barriers against non-Triticum blast pathotypes. These genes provide molecular targets for studying the interaction between pathogen effector and host resistance genes, and can potentially improve wheat resistance to blast fungus.
Article
Plant Sciences
Cezary Waszczak, Dmitry Yarmolinsky, Marina Leal Gavarron, Triin Vahisalu, Maija Sierla, Olena Zamora, Ross Carter, Tuomas Puukko, Nina Sipari, Airi Lamminmaki, Jorg Durner, Dieter Ernst, J. Barbro Winkler, Lars Paulin, Petri Auvinen, Andrew J. Fleming, Mats X. Andersson, Hannes Kollist, Jaakko Kangasjarvi
Summary: Land plants have evolved multiple adaptations to control transpiration, but the molecular mechanisms behind these adaptations are not fully understood. In this study, researchers used an ozone-sensitivity forward genetics approach to identify mutants in the plant Arabidopsis thaliana that are impaired in the regulation of gas exchange. Through experiments on these mutants, they found that a mutation in the MURUS1 gene led to high water loss and impaired leaf conductance in response to stomata-closing stimuli. They discovered that this high water loss was not due to stomatal movements but instead was linked to metabolic defects. The researchers also found that boron deficiency played a role in the water loss phenotype of the MURUS1 mutants, affecting the mechanical and morphological properties of stomatal complexes and overall plant physiology. This study highlights the importance of fucose metabolism and boron uptake in plant-water relations.