Article
Plant Sciences
Adam Kuzdralinski, Justyna Lesniowska-Nowak, Michal Nowak, Magdalena Kawecka, Anna Kot, Karolina Rozaniecka, Agnieszka Ostrowska, Marta Muszynska, Adam Wasko, Hubert Szczerba
Summary: New primer sets were developed for species-specific identification of Zymoseptoria tritici in wheat leaf samples, showing high specificity and sensitivity in both in silico validation and in vitro tests on common wheat samples with visual symptoms of Septoria tritici blotch conducted in Poland.
Article
Agronomy
Pierre Hellin, Maxime Duvivier, Thies M. Heick, Bart A. Fraaije, Charlotte Bataille, Aurelie Clinckemaillie, Anne Legreve, Lise N. Jorgensen, Bjorn Andersson, Berit Samils, Bernd Rodemann, Gunilla Berg, Fiona Hutton, Maxime Garnault, Moussa El Jarroudi, Gilles Couleaud, Steven Kildea
Summary: The study evaluated the frequency of key alterations causing resistance in commercial wheat crops across European countries, revealing the highest frequencies in Ireland and Great Britain, with a gradual increase over the past decade. While these alterations are becoming dominant in certain regions, there is still potential to delay their selection across the wider European population, highlighting the need for the implementation of fungicide antiresistance measures.
PEST MANAGEMENT SCIENCE
(2021)
Article
Plant Sciences
Hannah R. Blyth, Dan Smith, Robert King, Carlos Bayon, Tom Ashfield, Hannah Walpole, Eudri Venter, Rumiana V. Ray, Kostya Kanyuka, Jason J. Rudd
Summary: Mutagenomics screening on Zymoseptoria tritici identified four mutants with reduced virulence on wheat, and whole-genome re-sequencing revealed T-DNA insertion sites and other mutations potentially affecting gene functions. The loss of function mutations in the ZtSSK2 gene were found in two independent mutant strains with altered stress sensitivities and aberrant hyphal growth phenotypes, and genetic complementation restored their wild-type function. This study highlights the importance of whole genome sequencing in mutagenomic discovery and identifies new genes involved in pathogen virulence.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Sahbi Ferjaoui, Lamia Aouini, Rim B. Slimane, Karim Ammar, Suzanne Dreisigacker, Henk J. Schouten, Suraj Sapkota, Bochra A. Bahri, Sarrah Ben M'Barek, Richard G. F. Visser, Gert H. J. Kema, Sonia Hamza
Summary: This study identified broad resistance to Zymoseptoria tritici in the Tunisian durum wheat landrace accession 'Agili39' and mapped two major and two minor QTL associated with the resistance. The findings suggest that the natural pyramiding of these QTL in 'Agili 39' contributes to its broad efficacy against STB and can be utilized for effective breeding of Z. tritici resistance in durum wheat.
Article
Multidisciplinary Sciences
Samara Mejri, Alina Ghinet, Maryline Magnin-Robert, Beatrice Randoux, Cristina-Maria Abuhaie, Benoit Tisserant, Philippe Gautret, Benoit Rigo, Patrice Halama, Philippe Reignault, Ali Siah
Summary: The current global focus on agroecology and green agriculture requires the discovery of new ecofriendly and sustainable plant protection tools. This study synthesized 30 molecules from pyroglutamic acid, sourced from sugar beet byproducts, and examined their biological activity on wheat-Zymoseptoria tritici. The molecules provided significant disease protection when applied to the leaves, with up to 63% reduction in disease severity. Further investigation revealed that their activity was dependent on the activation of plant defense mechanisms. This study introduces a new family of bio-sourced resistance inducers and contributes to the development of sustainable agriculture.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Hadjer Bellah, Gwilherm Gazeau, Sandrine Gelisse, Reda Amezrou, Thierry C. Marcel, Daniel Croll
Summary: To predict future risks of crop pathogens, monitoring tools are needed to identify genetic changes in pathogen populations. This study developed a microfluidics-based gene sequencing method that can simultaneously detect 798 loci targeting virulence and fungicide resistance genes, as well as randomly selected genome-wide markers. The method showed stable and high success rates in different samples, both pure genomic DNA and material extracted from infected wheat leaves. The study also demonstrated the potential of the method to recover the pathogen population structure across French wheat fields.
Article
Biology
Ursula Oggenfuss, Thomas Badet, Thomas Wicker, Fanny E. Hartmann, Nikhil Kumar Singh, Leen Abraham, Petteri Karisto, Tiziana Vonlanthen, Christopher Mundt, Bruce A. McDonald, Daniel Croll
Summary: The study utilizes a global population genomics dataset to show that specific TE families have undergone substantial genome-wide expansion in different populations of the pathogen, possibly linked to colonization bottlenecks.
Article
Plant Sciences
Christopher Stephens, Fatih Olmez, Hannah Blyth, Megan McDonald, Anuradha Bansal, Emine Burcu Turgay, Florian Hahn, Cyrille Saintenac, Vladimir Nekrasov, Peter Solomon, Andrew Milgate, Bart Fraaije, Jason Rudd, Kostya Kanyuka
Summary: Septoria tritici blotch (STB), caused by the fungus Zymoseptoria tritici, is a major economically important disease of wheat. Recent research has identified gene-for-gene interaction factors between Z. tritici and wheat, namely the wheat receptor-like kinase Stb6 and the Z. tritici secreted effector protein AvrStb6. Studies show a high diversity of AvrStb6 haplotypes in earlier Z. tritici isolates, but a complete absence of the avirulence isoform among modern isolates, indicating a trend towards elimination of avirulence haplotypes in subsequent Z. tritici populations.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Multidisciplinary Sciences
Alice Feurtey, Cecile Lorrain, Megan C. McDonald, Andrew Milgate, Peter S. Solomon, Rachael Warren, Guido Puccetti, Gabriel Scalliet, Stefano F. F. Torriani, Lilian Gout, Thierry C. Marcel, Frederic Suffert, Julien Alassimone, Anna Lipzen, Yuko Yoshinaga, Christopher Daum, Kerrie Barry, Igor V. Grigoriev, Stephen B. Goodwin, Anne Genissel, Michael F. Seidl, Eva H. Stukenbrock, Marc-Henri Lebrun, Gert H. J. Kema, Bruce A. McDonald, Daniel Croll
Summary: Human activity affects the evolution of many species. Global trade of agricultural goods contributes to the spread of pathogens and alters their genetic makeup. Understanding how pathogens adapt and cope with new climates is crucial for predicting their impact on crop diseases.
NATURE COMMUNICATIONS
(2023)
Article
Genetics & Heredity
Elena Fantozzi, Sreedhar Kilaru, Sarah J. Gurr, Gero Steinberg
Summary: The fungus Zymoseptoria tritici causes Septoria tritici blotch of wheat. Time post inoculation shows asynchronous development stages co-existing in infected tissues, suggesting synchronization of pathogen population prior to sporulation. Later stages of pathogen development also occur asynchronously amongst the population of infecting cells, which should be considered when interpreting transcriptomics or proteomics data from infected plants.
FUNGAL GENETICS AND BIOLOGY
(2021)
Article
Agronomy
Ioannis Spyroglou, Krystyna Rybka, Pawel Czembor, Dominika Piaskowska, Marketa Pernisova, Przemyslaw Matysik
Summary: Fluorescence parameters can be used to estimate wheat STB resistance, and the resistance can be estimated within 8 days after inoculation.
Article
Plant Sciences
Yunqian Wang, Cong Liu, Yuanyuan Du, Kunyan Cai, Yanfeng Wang, Jia Guo, Xingxuan Bai, Zhensheng Kang, Jun Guo
Summary: This study identifies a Pst effector, PstSIE1, that targets the immune component TaSGT1 and is involved in suppressing wheat defense. Overexpression of PstSIE1 increases the number of rust pustules and reduces the accumulation of reactive oxygen species (ROS).
Article
Genetics & Heredity
Harry T. Child, Michael J. Deeks, Ken Haynes, Jason J. Rudd, Steven Bates
Summary: This study shows that autophagy does not contribute to the virulence of the fungal plant pathogen Zymoseptoria tritici. However, ZtATG8 plays an autophagy-independent role in the development of pathogenicity.
FUNGAL GENETICS AND BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Sreedhar Kilaru, Elena Fantozzi, Stuart Cannon, Martin Schuster, Thomas M. Chaloner, Celia Guiu-Aragones, Sarah J. Gurr, Gero Steinberg
Summary: Transitioning from spores to hyphae is critical for host invasion by Zymoseptoria tritici. This study reveals that the dimorphic switch can be initiated at low temperatures and is enhanced by wheat leaf surface compounds. Transcriptomics analysis identifies genes associated with the switch, and a genetic screen uncovers the important role of the white-collar complex in dimorphism and virulence.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Maxime Garnault, Clementine Duplaix, Pierre Leroux, Gilles Couleaud, Olivier David, Anne-Sophie Walker, Florence Carpentier
Summary: A study conducted in France found that the evolution of fungicide resistance is mainly determined by the regional scale of fungicide use. The results of the study suggest that collective management at the regional scale can effectively complement local actions.
Article
Ecology
Frederic Suffert, Ghislain Delestre, Sandrine Gelisse
Article
Ecology
Anne-Lise Boixel, Ghislain Delestre, Jean Legeay, Michael Chelle, Frederic Suffert
Article
Agronomy
D. Morais, C. Duplaix, I. Sache, V. Laval, F. Suffert, A. -S. Walker
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2019)
Article
Ecology
Lydie Kerdraon, Marie-Helene Balesdent, Matthieu Barret, Valerie Laval, Frederic Suffert
Article
Microbiology
Lydie Kerdraon, Matthieu Barret, Valerie Laval, Frederic Suffert
Article
Plant Sciences
Lydie Kerdraon, Matthieu Barret, Marie-Helene Balesdent, Frederic Suffert, Valerie Laval
MOLECULAR PLANT PATHOLOGY
(2020)
Article
Agronomy
Delphine Paumier, Blandine Bamme, Annette Penaud, Romain Valade, Frederic Suffert
Summary: The study in France investigated the dynamics of sexual reproduction in Mycosphaerella linicola, the causal agent of pasmo, during the interepidemic period. The sexual stage of M. linicola was found in plant host tissues for the first time in France, suggesting its significant role in pasmo epidemiology. The increase in flax cultivation area has led to a resurgence of M. linicola infections in recent years, with the sexual phase playing a crucial role in the disease cycle.
Article
Plant Sciences
Victor Vazquez-Carrasquer, Anne Laperche, Christine Bissuel-Belaygue, Michael Chelle, Celine Richard-Molard
Summary: Maintaining seed yield under low N inputs is a major challenge for breeding, and understanding the genetic diversity related to Nitrogen Use Efficiency (NUE) is crucial. This study found that Nitrogen Uptake Efficiency (NUpE) and fine-root growth play important roles in optimizing NUE, providing new prospects for breeding.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agronomy
Frederic Suffert, Muriel Suffert
Summary: This article presents the experience of conducting research related to plant diseases in 2020, focusing on the diversity and beauty of fungal plant pathogens. Through phytopathological strolls during the lockdown in France, the authors observed and described numerous fungal pathogens, shared their findings on Twitter, and emphasized the importance of promoting the search, description, and conservation of plant pathogens.
Article
Agronomy
Carolina Orellana-Torrejon, Tiphaine Vidal, Anne-Lise Boixel, Sandrine Gelisse, Sebastien Saint-Jean, Suffert Frederic
Summary: Cultivar mixtures can slow down polycyclic epidemics and have a reciprocal protective effect on disease reduction. The proportions of cultivars in mixtures can modulate the size of pathogen population, the size of virulent subpopulation, and the frequency of virulence.
Article
Biodiversity Conservation
Valerie Laval, Lydie Kerdraon, Matthieu Barret, Anne-Lise Liabot, Coralie Marais, Benjamin Boudier, Marie-Helene Balesdent, Marion Fischer-Le Saux, Frederic Suffert
Summary: This study utilized both culture-dependent and culture-independent methods to characterize the microbiota diversity on wheat and oilseed rape residues. The research aimed to develop a culture methodology for establishing synthetic crop residue microbial communities, focusing on testing potential interactions and beneficial taxa against plant pathogens. The study found a higher bacterial diversity compared to fungal diversity, with isolation and metabarcoding providing consistent and complementary information on microbial community profiles.
Article
Ecology
Anne-Lise Boixel, Michael Chelle, Frederic Suffert
Summary: This study investigated the diversity of thermal responses in populations of the wheat pathogen Zymoseptoria tritici sampled from different climatic conditions. The results showed geographic variation in thermal response among populations, with major alterations due to seasonal patterns, and individual plasticity and variation in sensitivity to temperature conditions.
ECOLOGY AND EVOLUTION
(2022)
Article
Agronomy
Frederic Bernard, Michael Chelle, Alain Fortineau, Ons Riahi El Kamel, Sylvain Pincebourde, Ivan Sache, Frederic Suffert
Summary: Based on thermal ecology studies, foliar fungal pathogens respond directly to leaf temperature fluctuations, and large thermal amplitudes have detrimental effects on pathogen fitness.
AGRICULTURAL AND FOREST METEOROLOGY
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
L. Kerdraon, M. H. Balesdent, M. Barret, F. Suffert, V. Laval
MOLECULAR PLANT-MICROBE INTERACTIONS
(2019)
Review
Plant Sciences
Lydie Kerdraon, Valerie Laval, Frederic Suffert
PHYTOBIOMES JOURNAL
(2019)