Article
Biochemistry & Molecular Biology
Pingzhou Du, Yu Liu, Lu Deng, Dong Qian, Xiuhua Xue, Ting Yang, Tonghui Li, Yun Xiang, Haiyun Ren
Summary: The newly discovered AtMAC protein is involved in the regulation of phragmoplast organization during centrifugal phragmoplast expansion, playing a crucial role in cytokinesis.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Biology
Xiaohang Cheng, Magdalena Bezanilla
Summary: By studying the role of SABRE in moss plants, it was found that SABRE plays a crucial role in cell growth and division processes, and the endoplasmic reticulum plays an important regulatory role in cell division.
Article
Plant Sciences
Andrzej Kazmierczak, Ewa Siatkowska, Ruoxi Li, Sophie Bothe, Peter Nick
Summary: Plant cells can undergo regulated cell death in response to exogenous factors or as a regular element of development. The cellular aspects of these death responses differ, indicating differences in early signaling. This study uses cytokinin-induced programmed cell death as a paradigm to investigate the role of the cytoskeleton in developmentally induced cell death.
Article
Multidisciplinary Sciences
Simon Gemble, Rene Wardenaar, Kristina Keuper, Nishit Srivastava, Maddalena Nano, Anne-Sophie Mace, Andrea E. Tijhuis, Sara Vanessa Bernhard, Diana C. J. Spierings, Anthony Simon, Oumou Goundiam, Helfrid Hochegger, Matthieu Piel, Floris Foijer, Zuzana Storchova, Renata Basto
Summary: This study reveals that human cells experience high levels of DNA damage during DNA replication in the first S phase following tetraploidy induction. These damages are caused by protein shortage during the G1/S transition, resulting in inaccurate DNA replication. These findings provide an explanation for the genetic instability landscape that favors tumorigenesis after tetraploidization.
Article
Biochemistry & Molecular Biology
Emmanuel Panteris, Anna Kouskouveli, Dimitris Pappas, Ioannis-Dimosthenis S. Adamakis
Summary: The malfunction of p60-katanin in cell plate/daughter wall formation in the fra2 Arabidopsis thaliana loss-of-function mutant was investigated. Deviations in the chemical composition of cell plate/new cell wall were identified by transmission electron microscopy (TEM), immunolabeling, and confocal microscopy. Delayed cytokinesis in the mutant results in a loss of synchronization between cell plate growth and chemical maturation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Hong-Yun Zeng, Yu Liu, Ding-Kang Chen, He-Nan Bao, Li-Qun Huang, Jian Yin, Yi-Li Chen, Shi Xiao, Nan Yao
Summary: The study revealed that EDS1 and PAD4 are involved in plant sphingolipid metabolism and associated cell death.
Article
Biochemistry & Molecular Biology
Xuerui Lu, Shixi Shi, Chong Wu, Xueao Zheng, Chenkun Yang, Jie Luo, Shunping Yan
Summary: A study reveals that the shikimate pathway promotes programmed cell death (PCD) in Arabidopsis, and the accumulation of shikimate may explain the action of the successful herbicide glyphosate.
JOURNAL OF GENETICS AND GENOMICS
(2022)
Article
Cell Biology
Xiao-Yan Fan, Xing-Hua Wang, Feng-Yun Xie, Jun-Yu Ma, Xiang-Hong Ou, Shi-Ming Luo
Summary: This study demonstrates that the first cytokinesis in mouse embryos is completed about 15 hours after mitosis and relies on microtubules instead of microfilaments. These findings are crucial for understanding the mechanisms of mouse embryo development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Ondrej Kucera, Valerie Siahaan, Daniel Janda, Sietske H. Dijkstra, Eliska Pilatova, Eva Zatecka, Stefan Diez, Marcus Braun, Zdenek Lansky
Summary: Cytokinetic ring constriction during cell division requires actin but curiously is independent of myosin in many organisms. Here, the authors show that anillin, a protein enriched in the contractile ring, is a non-motor actin crosslinker that generates contractile force in lieu of a molecular motor. By maximizing the lengths of overlaps between bundled actin filaments, anillin autonomously propels the contractility of actin bundles.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Matthias Van Durme, Yadira Olvera- Carrillo, Marie L. Pfeiffer, Nicolas M. Doll, Freya De Winter, Zongcheng Lin, Moritz K. Nowack
Summary: Flowers have a specific fertile period in which pollination and fertilization must occur for seed and fruit development to begin. Unpollinated flowers can remain receptive for a few hours or several weeks before becoming infertile. Floral longevity, specifically the lifespan of the ovule, is influenced by natural selection and plant breeding. The aging process of unfertilized ovules in Arabidopsis thaliana exhibits characteristics of programmed cell death, with significant transcriptomic changes. Up-regulated transcription factors, such as NAP/ANAC029, SHYG/ANAC047, and ORE1/ANAC092, regulate ovule senescence and can extend fertility in Arabidopsis ovules.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Plant Sciences
Rory Burke, Aideen McCabe, Neetu Ramesh Sonawane, Meet Hasmukh Rathod, Conor V. Whelan, Paul F. McCabe, Joanna Kacprzyk
Summary: Programmed cell death (PCD) is a genetically controlled process that eliminates redundant, damaged, or infected cells. This study used RNA sequencing and Arabidopsis thaliana suspension cells to identify PCD-associated genes and infer regulatory networks of PCD in plants.
Article
Plant Sciences
Jianyan Zeng, Jing Xi, Baoxia Li, Xingying Yan, Yonglu Dai, Yiping Wu, Yuehua Xiao, Yan Pei, Mi Zhang
Summary: This study elucidates the roles of actin and microtubule cytoskeletons in the initiation of cotton fiber growth. Both cytoskeletons align along the growth axis in initiating fibers. Dense and bundled actin filaments (AFs) are observed in fiber cells, while microtubules (MTs) are randomized. Pharmacological experiments demonstrate that depolymerization of both AFs and MTs assist fiber initiation, while AF stabilization and depolymerization inhibit elongation. Notably, the organization of AFs is correlated with MT dynamics.
PLANT CELL REPORTS
(2022)
Review
Chemistry, Physical
Qi Meng, Binbin Ding, Ping'an Ma, Jun Lin
Summary: Programmed cell death (PCD) and immunogenic cell death (ICD) are important mechanisms in cancer therapy, and understanding their relationship is significant for tumor treatments. This review describes the interrelationship between PCD and ICD using nanomedicines as examples. It provides an overview of PCD patterns, explores the link between apoptosis and ICD, introduces non-apoptotic signaling pathways and their relationship with ICD, and summarizes the potential application of PCD and ICD in the development of new nanomaterials. The review aims to deepen understanding, extend biomedical applications, and promote progress in clinical tumor therapy.
Review
Cell Biology
Shyama Nandakumar, Emily Rozich, Laura Buttitta
Summary: Polyploidy in the nervous system is more common than previously thought and can have a protective role under normal physiological conditions. However, cell cycle reentry in neurons is linked to neurodegenerative conditions and considered detrimental to neuronal health.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Anthea Weng, Mariana Maciel Herrerias, Satoshi Watanabe, Lynn C. Welch, Annette S. Flozak, Rogan A. Grant, Raul Piseaux Aillon, Laura A. Dada, Seung Hye Han, Monique Hinchcliff, Alexander Misharin, G. R. Scott Budinger, Cara J. Gottardi
Summary: This study reveals that lung injury leads to hypertrophy and polyploidy of alveolar epithelial cells. The small molecule ISRIB can attenuate the hypertrophic and polyploid features of these cells. These findings suggest a potential link between alveolar epithelial injury and hypertrophic growth and polyploidization of the cells.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Infectious Diseases
Idris Mhaidi, Mouad Ait Kbaich, Sofia El Kacem, Othmane Daoui, Khadija Akarid, Tatiana Spitzova, Petr Halada, Vit Dvorak, Meryem Lemrani
Summary: This study in Morocco identified sand fly fauna in Ibaraghen village and confirmed the role of P. sergenti as a vector in the Azilal focus of cutaneous leishmaniasis. The relative abundance of other potential vector species also emphasizes the risk of introducing L. infantum and L. major in the province. This is the first combined approach in Morocco to identify sand flies using both morphology and molecular methods.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Chemistry, Analytical
Marek Polak, Ghazaleh Yassaghi, Daniel Kavan, Frantisek Filandr, Jan Fiala, Zdenek Kukacka, Petr Halada, Dmitry S. Loginov, Petr Novak
Summary: The combination of covalent labeling techniques with mass spectrometry is a progressive approach for studying protein surfaces and protein-ligand interactions. In this study, the interaction interface between the DNA binding domain of the FOXO4 protein and the DNA binding element was mapped. Different approaches were used to confirm the results and compare with other studies, showing consistent findings. This method is important for understanding the structure and dynamics of protein-DNA interactions.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Aline Fernandes de Oliveira, Milan Svoboda, Oldrich Benada, Jan Kratzer
Summary: A fast and simple preconcentration step was developed for ultratrace arsenic determination using a quartz modular trap-and-atomizer device. Gold nanoparticles were found to be the most effective preconcentration surface, achieving 90% efficiency under optimized conditions. The method was successfully validated for arsenic determination in various reference materials.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2022)
Article
Biotechnology & Applied Microbiology
Evelina Gablech, Zdenka Fohlerova, Karel Svec, Frantisek Zales, Oldrich Benada, Olga Kofronova, Jana Pekarkova, Ondrej Caha, Imrich Gablech, Jiri Gabriel, Jana Drbohlavova
Summary: The study prepared PVP-Se NPs through wet chemical reduction and characterized their physical and chemical properties using various analytical methods, while also evaluating their toxicological properties. In antifungal experiments, the combination of PVP-Se NPs with a commercial fungicide showed better results than using either alone, demonstrating their synergistic effect and inhibitory effect on fungal growth.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2022)
Article
Biochemistry & Molecular Biology
Alina Vaitsiankova, Kamila Burdova, Margarita Sobol, Amit Gautam, Oldrich Benada, Hana Hanzlikova, Keith W. Caldecott
Summary: PARP inhibitors hinder the maturation of nascent DNA strands during DNA replication, particularly unligated Okazaki fragments and other discontinuities, resulting in cytotoxic effects. The activation of PARP1 is elevated in cells lacking the FEN1 nuclease, suggesting its involvement in the detection and processing of these DNA replication intermediates. PARP inhibitors disrupt the integrity of nascent DNA strands in both normal and FEN1(-/-) cells, leading to the formation of single-strand nicks or gaps. These findings highlight the importance of unligated Okazaki fragments and other discontinuities in the cytotoxicity of PARP inhibitors.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2022)
Article
Environmental Sciences
Monika Danchenko, Oldrich Benada, L'udovit Skultety, Zuzana Sekeyova
Summary: In this study, a strain of Rickettsia felis was successfully isolated and characterized from a common tick species in Slovakia. It is the first time that R. felis has been isolated from ticks, and further research on its growth properties and gene sequences revealed no nucleotide polymorphisms compared to known strains, but the plasmid pRF delta was absent in this isolate.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Microbiology
Viola Vankova Hausnerova, Olga Marvalova, Michaela Sikova, Mahmoud Shoman, Jarmila Havelkova, Milada Kambova, Martina Janouskova, Dilip Kumar, Petr Halada, Marek Schwarz, Libor Krasny, Jarmila Hnilicova, Josef Panek
Summary: Bacteria use small non-coding RNAs to regulate gene expression. The study identified a specific RNA called Ms1 in Actinobacteria that binds to the core of RNA polymerase and affects its intracellular level. Through computational search and gene synteny approach, the study also found Ms1 RNA candidates in other Actinobacteria, demonstrating the widespread presence of Ms1 RNA as a class of RNAP binding sRNAs.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Parasitology
Nacer Eddine Messahel, Kamel Eddine Benallal, Petr Halada, Ismail Lafri, Hemza Manseur, Ahcene Hakem, Karim Houali, Zoubir Harrat, Petr Volf, Vit Dvorak
Summary: Leishmaniases are neglected vector-borne diseases. This study in Algeria revealed the feeding preference of sand flies for cattle and the presence of cattle and dog blood in sand flies, which could play an important role in parasite transmission.
VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS
(2022)
Article
Chemistry, Analytical
Ghazaleh Yassaghi, Zdenek Kukacka, Jan Fiala, Daniel Kavan, Petr Halada, Michael Volny, Petr Novak
Summary: Fast photochemical oxidation of proteins (FPOP) footprinting is a method that uses chemical reactions to label protein structures. It provides information about protein conformation, dynamics, and interactions. FPOP samples can be analyzed using either bottom-up or top-down mass spectrometry approaches. The top-down approach allows for more specific selection of protein subpopulations, but is limited to smaller proteins. Comparison with bottom-up data confirms the usefulness of the top-down approach in oxidative protein footprinting.
ANALYTICAL CHEMISTRY
(2022)
Article
Cell Biology
Andi Alijagic, Nikolai Scherbak, Oleksandr Kotlyar, Patrik Karlsson, Xuying Wang, Inger Odnevall, Oldrich Benada, Ali Amiryousefi, Lena Andersson, Alexander Persson, Jenny Felth, Henrik Andersson, Maria Larsson, Alexander Hedbrant, Samira Salihovic, Tuulia Hyoetylaeinen, Dirk Repsilber, Eva Saerndahl, Magnus Engwall
Summary: The physicochemical characterization of AMPs revealed their complexity in size, shape, and chemistry. The AMPs exhibited concentration-dependent effects on the cytoskeleton, mitochondria, and cell membranes. Metabolomics and lipidomics analysis provided insight into the inflammation-related metabolites and extensive membrane remodeling induced by AMPs. Overall, AMP exposure resulted in internalization, oxidative stress, cytoskeleton disruption, mitochondrial activation, membrane remodeling, and metabolic reprogramming of lung epithelial cells and macrophages. Integrating Cell Painting with metabolomics and lipidomics offers an advanced nanosafety methodology to capture cellular and molecular phenotypes and understand the biological mechanisms of (nano)particle exposure.
Article
Biochemistry & Molecular Biology
Jana Bibova, Veronika Kabrtova, Veronika Vecerova, Zuzana Kucerova, Martin Hudecek, Lenka Plackova, Ondrej Novak, Miroslav Strnad, Ondrej Plihal
Summary: Plasmodiophora brassicae is a pathogen that causes clubroot disease in cruciferous plants, leading to morphological changes and symptoms such as gall formation and leaf abscission. The homeostasis of cytokinin and auxin appears to be crucial for club development. Study on late-stage infected Arabidopsis thaliana showed changes in cytokinin levels and their role in disease progression.
Article
Microbiology
Jan Bavlovic, Ivona Pavkova, Lucie Balonova, Oldrich Benada, Jiri Stulik, Jana Klimentova
Summary: Francisella tularensis is a highly infectious bacterium that causes tularemia. Bacterial outer membrane vesicles (OMVs) of Francisella play a role in host-pathogen interactions and have potential use in a subunit vaccine. This study focused on the secretion of OMVs in O-antigen mutant strains and found that the presence of LPS and the O-antigen capsule is critical for the virulence and tubular shape of OMVs.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Oncology
Vera Chvalova, Vignesh Venkadasubramanian, Zuzana Klimova, Jana Vojtova, Oldrich Benada, Ondrej Vanatko, Tomas Vomastek, Tomas Grousl
Summary: This study used CRISPR/Cas9 and siRNA to decrease the expression of RACK1 in MDCK epithelial cells and Rat2 fibroblasts. The depletion of RACK1 resulted in decreased cell proliferation, increased cell area and perimeter, and the appearance of large binucleated cells, indicating a defect in cell cycle progression. These findings demonstrate the essential role of RACK1 in both epithelial and mesenchymal cell lines.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Microbiology
Tereza Jurikova, Hynek Macha, Vanda Lupjanova, Tomas Pluhacek, Helena Maresova, Barbora Papouskova, Dominika Luptakova, Rutuja H. Patil, Oldrich Benada, Michal Grulich, Andrea Palyzova
Summary: Pseudomonas aeruginosa is a significant pathogen in hospital-acquired infections, often collaborating with the opportunistic fungal pathogen Aspergillus fumigatus in respiratory infections. This study identifies a potential new biomarker, QS molecule portfolio, which affects virulence factor secretion and biofilm formation in P. aeruginosa. The study also demonstrates changes in QS molecule levels during the transition from planktonic to sessile cells and in co-cultures with A. fumigatus.
Article
Materials Science, Textiles
Michal Syrovy, Pavla Capkova, Petr Rysanek, Oldrich Benada, Monika Vostinakova
Summary: The manufacturing of membranes for CO2 capture is an important research topic. This study aims to achieve maximum CO2 sorption capacity while maintaining air permeability with minimal technological steps. Electrospun nanofibrous membranes PAN/TETA and PAN/TEPA were prepared using one-step technology and two different spinning conditions - needle spinning and wire spinning. The best result was obtained for wire spinning PAN_TEPA_2% with a CO2 sorption capacity of 11.7 +/- 1.3 cm(3)/g and air permeability of 53 +/- 5 L/m(2)/s, providing potential for practical use.
JOURNAL OF INDUSTRIAL TEXTILES
(2023)