Article
Plant Sciences
Christoph-Martin Geilfus, Xudong Zhang, Axel Mithoefer, Lisa Burgel, Gyoengyi Bardos, Christian Zoerb
Summary: The chloride component of NaCl salinity leads to transient alkalinization of the leaf apoplast, which initiates stomatal closure by affecting the pH(apo) and subsequently the transcription of ABA, independently from other chloride stress-induced effects.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Review
Plant Sciences
Francisco M. M. Gamez-Arjona, Clara Sanchez-Rodriguez, Juan Carlos Montesinos
Summary: This review examines the variations in root apoplastic pH and their impact on plant response. The pH in the root apoplast is regulated by external cues and can influence the plant's ability to adapt to detrimental factors through crosstalk between different mechanisms.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Yang Shao, Xiaohui Feng, Hiroki Nakahara, Muhammad Irshad, A. Egrinya Eneji, Yuanrun Zheng, Haruyuki Fujimaki, Ping An
Summary: Root growth in plants is closely linked to salt tolerance, with a significant impact of apoplastic pH and cell wall extensibility on the elongation zone under salinity stress. Expansins play a crucial role in regulating cell wall extensibility at different pH levels, demonstrating potential for enhancing plant growth under saline conditions.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Plant Sciences
Quang Tran, Kenji Osabe, Tetsuyuki Entani, Tetsuichi Wazawa, Mitsuru Hattori, Takeharu Nagai
Summary: The acidity of root apoplastic fluid is crucial for the growth of plant roots. This study developed a novel pH indicator and successfully quantified the pH of root apoplastic fluid, providing evidence for the acid growth theory.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Amit Sagervanshi, Christoph-Martin Geilfus, Hartmut Kaiser, Karl H. Muehling
Summary: The mechanisms by which plants respond to alkali salt stress and the role of alkaline pH in this process are still unclear. This study investigates the fast responses specific to alkaline pH and alkali salt stress in hydroponically grown Vicia faba L. plants. The findings suggest that alkaline pH and alkali salt stress induce changes in apoplastic pH, physiological responses, and gene regulation, which are important for early plant adaptation to alkali salt stress.
Article
Biochemical Research Methods
Hartmut Kaiser, Amit Sagervanshi, Karl H. Muehling
Summary: This study presents a method to measure and manipulate leaf hydration by controlling leaf water content. The technique allows precise control of leaf water content and enables the investigation of quantitative relationships between water content and signaling and regulatory processes.
Article
Plant Sciences
Bastian Leander Franzisky, Christoph-Martin Geilfus, Maria Luisa Romo-Perez, Ines Fehrle, Alexander Erban, Joachim Kopka, Christian Zorb
Summary: Stomatal movements are influenced by changes in guard cell turgor, which are facilitated by the accumulation of ions in guard cells. Exposure to NaCl leads to changes in Na+ and Cl- concentrations in guard cells, while their metabolism remains largely unaffected by salt ion concentrations. Additionally, the accumulation of stress-related photosynthate in leaves contributes to the permanent closing response of stomata under stress.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Plant Sciences
Natalya I. Rumyantseva, Alfia I. Valieva, Yulia A. Kostyukova, Marina V. Ageeva
Summary: This study successfully developed a VIC technique for AWF isolation from Tartary buckwheat leaves, revealing that in vitro leaves are more suitable for VIC compared to in vivo leaves. The morphological, anatomical, and physiological characteristics of in vitro leaves differ significantly, and the size of stomata pores is the key factor for isolating AWF free of chlorophyll contamination.
Review
Plant Sciences
Thorsten Seidel
Summary: V-ATPase is the dominant proton pump in plant cells, playing crucial roles in cytosolic pH homeostasis, endomembrane transport processes, and stress responses. Its complex structure and multiple subunit isoforms provide structural flexibility, but also require sophisticated assembly machinery and transport routes in cells.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Satoru N. Kinoshita, Toshinori Kinoshita
Summary: Cytosolic and extracellular pH homeostasis is vital for various physiological processes in plants. The plasma membrane H+-ATPase plays a critical role in maintaining pH homeostasis and its activity is regulated by protein abundance and post-translational modifications. Phosphorylation of the penultimate threonine of the carboxy terminus can activate the plasma membrane H+-ATPase. This review summarizes recent findings on the regulation of plasma membrane H+-ATPase in photosynthetic tissues of plants, with a focus on its mechanisms and physiological roles.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Cell Biology
Michelle E. Maxson, Yazan M. Abbas, Jing Ze Wu, Jonathan D. Plumb, Sergio Grinstein, John L. Rubinstein
Summary: This study shows that proton-pumping vacuolar H+ ATPases (V-ATPases) are responsible for the acidic environment in secretory and endocytic organelles. The researchers developed a new probe derived from SidK protein to visualize and quantify V-ATPases with high specificity. They found that the density of V-ATPases increases during phagosome maturation and their distribution varies in lysosomes depending on their subcellular localization.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biology
Wei Li, Kosuke Kawaguchi, Sunao Tanaka, Chenfeng He, Yurina Maeshima, Eiji Suzuki, Masakazu Toi
Summary: This study reports the regulatory role of adenosine triphosphatase H+ transporting accessory protein 2 (ATP6AP2) in senescent breast cancer cells. A decline in ATP6AP2 triggers aberrant pH levels, impairing lysosomal function and causing immune profile changes in senescent cells.
COMMUNICATIONS BIOLOGY
(2023)
Article
Plant Sciences
Heike M. Mueller, Bastian L. Franzisky, Maxim Messerer, Baoguo Du, Thomas Lux, Philip J. White, Sebastien Christian Carpentier, Jana Barbro Winkler, Joerg-Peter Schnitzler, Hamed A. El-Serehy, Khaled A. S. Al-Rasheid, Naif Al-Harbi, Saleh Alfarraj, Jorg Kudla, Jaakko Kangasjarvi, Michael Reichelt, Axel Mithofer, Klaus F. X. Mayer, Heinz Rennenberg, Peter Ache, Rainer Hedrich, Christoph-Martin Geilfus
Summary: Date palm can grow and complete its life cycle in highly saline soils by efficiently combining salt-tolerance mechanisms such as sodium and chloride exclusion at the roots, osmotic adjustment and reactive oxygen species scavenging in leaves, and remodeling of the ribosome-associated proteome in salt-exposed root cells. The excellent salt stress tolerance of date palm is explained by the effective combination of these mechanisms.
Article
Veterinary Sciences
Walaa F. A. Emeish, Soha A. Soliman, Ahmed Ezzat Ahmed, Aiman A. Alsaegh, Khalid M. Alsyaad, Montaser Elsayed Ali, Hassan Ahmed
Summary: This study investigated the osmoregulatory response of common carp to salinity increases by examining the mRNA expression of NKA alpha 3 and AQP3 alpha genes and histomorphological changes of the chloride cells in their gills. The results showed that the mRNA expression levels of NKA alpha 3 and AQP3 alpha were up-regulated with increasing NaCl concentration, and the gill chloride cells displayed significant histomorphological alterations. These findings are important for future cultivation of common carp in high salinity water, especially in countries with scarce freshwater resources.
PAKISTAN VETERINARY JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Lin Liu, Tong Jiang, Jia Zhou, Yikun Mei, Jinyang Li, Jingcong Tan, Luqi Wei, Jingquan Li, Yibing Peng, Changbin Chen, Ning-Ning Liu, Hui Wang
Summary: Fungal infections pose a major global threat to human health, with drug resistance and limited antifungal agents complicating the situation. The study found that ponatinib enhances the activity of fluconazole against a variety of fungal pathogens, suggesting it as a novel antifungal agent with broad-spectrum synergistic interactions.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Agronomy
Yafei Guo, Asif Naeem, Stefan Becker-Fazekas, Britta Pitann, Karl H. Muehling
Summary: The study found that the efficacy of different NI in inhibiting N2O emissions in sandy soil is influenced by soil temperature and moisture levels, with DMPP and PIADIN showing stronger inhibition effects on the emissions.
JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE
(2022)
Article
Biochemical Research Methods
Hartmut Kaiser, Amit Sagervanshi, Karl H. Muehling
Summary: This study presents a method to measure and manipulate leaf hydration by controlling leaf water content. The technique allows precise control of leaf water content and enables the investigation of quantitative relationships between water content and signaling and regulatory processes.
Article
Biochemistry & Molecular Biology
Amit Sagervanshi, Christoph-Martin Geilfus, Hartmut Kaiser, Karl H. Muehling
Summary: The mechanisms by which plants respond to alkali salt stress and the role of alkaline pH in this process are still unclear. This study investigates the fast responses specific to alkaline pH and alkali salt stress in hydroponically grown Vicia faba L. plants. The findings suggest that alkaline pH and alkali salt stress induce changes in apoplastic pH, physiological responses, and gene regulation, which are important for early plant adaptation to alkali salt stress.
Article
Plant Sciences
O. S. Olawuwo, M. A. Abdalla, K. H. Muhling, L. J. McGaw
Summary: The study found that plant feed additives have high nutritional value, can improve poultry digestion, reduce disease incidence, and exhibit good antioxidant activity. The results show that the concentrations of macro- and micronutrients in plant powders are higher, indicating their potential as alternatives to antibiotic feed additives.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Plant Sciences
Tania T. Korber, Noah Frantz, Tobias Sitz, Muna A. Abdalla, Karl H. Muehling, Sascha Rohn
Summary: Alterations in chloroplast membrane lipids can serve as indicators of changes induced by environmental and nutritional factors. Changes in fatty acid saturation degree are a strategy used by plants to adapt to abiotic stress. This study found that high sulfur fertilization affected the content and composition of sulfoquinovosyl diacylglycerol derivatives (SQDG), as well as the overall lipid composition of the chloroplast membrane.
Article
Plant Sciences
Muna Ali Abdalla, Christine Lentz, Karl H. Muehling
Summary: This study investigated the beneficial effects of selenium (Se) and sulfur (S) enrichment on the primary metabolism in butterhead lettuce. The results showed that the interaction between Se and S promoted the synthesis of amino acids and soluble sugars, which could have important implications for the nutritional value and quality of lettuce plants.
Article
Agronomy
Ghulam Jillani, Saad Sulieman, Karl H. Muehling
Summary: This study found that faba bean has a strong capacity to utilize organic phosphorus substrates, and this utilization is closely related to its nodules structure and efficient utilization of carbohydrate budget. The preferential allocation of phosphate to nodules is essential for plant growth under these circumstances.
JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE
(2022)
Review
Plant Sciences
Muna Ali Abdalla, Karl H. Muehling
Summary: Selenium's classification as an essential nutrient for plant growth is still uncertain, but its role in human health is important due to its impact on the production of primary and secondary metabolites. Low levels of selenium have been linked to cancer and cardiac diseases. Enriching crops with selenium has been established as a pioneering strategy to increase selenium intake in animals and humans. Studies have shown that selenium biofortification can increase selenium levels in plant tissues without affecting productivity or quality. However, the accumulation of selenium in plants needs to be quantified and balanced, as it may affect nutrient balance. This timely review provides an overview of the metabolic changes induced in plants by selenium enrichment, and highlights the contrasting effects on various secondary metabolites.
CRITICAL REVIEWS IN PLANT SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Tania T. Koerber, Tobias Sitz, Muna A. Abdalla, Karl H. Muehling, Sascha Rohn
Summary: Sulfur deprivation in plants has a significant impact on membrane lipids, resulting in abiotic stress. Different levels of sulfur were used to identify individual thylakoid membrane lipids in lettuce plants. LC-ESI-MS/MS was used to analyze the changes in lipids and understand the plant's response to stress. The results showed transformations in glycolipids, increased lipid saturation, and higher levels of oxidized SQDG under sulfur-limiting conditions. The oxidized SQDG could potentially serve as a marker for other abiotic stress factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Md Arif Hussain, Asif Naeem, Britta Pitann, Karl H. Muehling
Summary: Waterlogging has a significant impact on plant growth and development, leading to reduced yield. Supplementing high sulphur (S) levels can alleviate the adverse effects of waterlogging stress on oilseed rape by improving growth and yield through enhanced antioxidant activities. However, under normal conditions, high S levels do not provide additional benefits compared to medium S application. Therefore, additional S supplementation can be employed as a potential strategy to mitigate the negative effects of waterlogging stress.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Review
Soil Science
Pablo Lacerda Ribeiro, Filipe Selau Carlos, Gabriel Barth, Karl H. Muhling
Summary: Meta-analysis of 50 articles found that the use of nitrification inhibitors (NIs) can increase crop yield and soil NH4+ concentrations, while reducing N2O emissions and soil NO3- concentrations, with no effect on ammonia volatilization. The effectiveness of NIs in tropical agroecosystems in Brazil is similar to previous meta-analyses conducted in temperate climates, indicating that NI application is an efficient strategy to delay nitrification and mitigate N2O emissions.
NUTRIENT CYCLING IN AGROECOSYSTEMS
(2023)
Article
Plant Sciences
Jon Niklas Henningsen, Hector Alejandro Bahamonde, Karl Hermann Muehling, Victoria Fernandez
Summary: Foliar fertilisation is an important technique in agriculture, but the mechanisms of foliar uptake are not well understood. In this study, tomato and pepper plants with different leaf surface traits were used to investigate the importance of leaf surface features for foliar phosphorus (P) uptake. The results showed that the presence of trichomes on tomato leaf veins contributed to increased P concentration, while the abaxial side of pepper leaves had the highest P absorption rate. These findings suggest that different leaf parts play unequal roles in the absorption of foliar-applied agrochemicals.
Article
Biochemistry & Molecular Biology
Kashif Saeed, Fatiha Kalam Nisa, Muna Ali Abdalla, Karl Hermann Muehling
Summary: The study found that selenium treatments caused significant changes in plant biomass and examined element concentrations under sulfur deprivation or enrichment conditions. Selenium promoted sulfur accumulation in sulfur-treated plants and had a beneficial impact on minerals in roots grown under sulfur-limiting conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Frank K. Amoako, Saad Sulieman, Karl H. Muehling
Summary: This study investigates the mineral and carbon metabolism of faba bean nodules under different phosphate conditions. The results show similar growth patterns and biomass partitioning between phosphate and phytate treatments, both differing substantially from the phosphate-deficient treatment. Additionally, the overall elemental phosphorus concentrations in both phosphate and phytate treatments significantly increase compared to the phosphate-deficient treatment. Metabolite analysis reveals no significant differences between phosphate and phytate treatments.
Article
Agronomy
Frank K. Amoako, Ghulam Jillani, Saad Sulieman, Karl H. Muehling
Summary: This study investigated the organic phosphorus use efficiency of eight varieties of faba bean. The results showed that although plants grown with organic phosphorus and inorganic phosphorus produced similar amounts of dry matter, the phosphorus uptake by inorganic phosphorus-fertilized plants was twice as high as that by organic phosphorus-fertilized plants. Additionally, significant differences were observed between organic phosphorus and inorganic phosphorus in terms of nodulation characteristics and phosphorus utilization efficiency. Among the tested varieties, Tiffany had higher organic phosphorus utilization efficiency and organic phosphorus beneficiary factor.
JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE
(2023)