Article
Cell & Tissue Engineering
Behnoosh Tayebi, Mahnaz Babaahmadi, Mohammad Pakzad, Mostafa Hajinasrollah, Farhad Mostafaei, Shahrbanoo Jahangiri, Amir Kamali, Hossein Baharvand, Mohamadreza Baghaban Eslaminejad, Seyedeh-Nafiseh Hassani, Ensiyeh Hajizadeh-Saffar
Summary: Mesenchymal stromal cells (MSCs) have shown potential in treating inflammatory and non-inflammatory diseases, but their clinical applications require strict evaluations and monitoring to ensure compliance with safety standards. This study aimed to assess the potential toxicity effects of cryopreserved human bone marrow-derived clonal MSCs in animal models during different time periods.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Multidisciplinary Sciences
Alicia Sanchez-Mata, Elena Gonzalez-Munoz
Summary: This review outlines the origin, cell identity markers, and immunomodulatory properties of MenSCs, aiming to enhance understanding of their characteristics and identify potential avenues for exploration.
Article
Oncology
Rafaela Rossetti, Felipe Augusto Ros, Lucas Eduardo Botelho de Souza, Juliana de Matos Maconetto, Pericles Natan Mendes da Costa, Fernanda Ursoli Ferreira, Josiane Serrano Borges, Julianne Vargas de Carvalho, Nayara Patricia Morotti, Simone Kashima, Dimas Tadeu Covas
Summary: The bone marrow microenvironment may contain two distinct populations of mesenchymal stromal cells (MSC), one located perivascularly and the other in the endosteum. Recent research has focused on better understanding these MSC subsets in order to improve their clinical applications. Studies indicate that compact bone is a rich source of MSC and offers lower risk of contamination by hematopoietic cells. Compact bone-derived MSC also show higher osteogenic potential compared to bone marrow-derived MSC. These findings suggest the presence of functionally different MSC populations in bone marrow and compact bone.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Adrienne Wright, Orman (Larry) Snyder, Hong He, Lane K. Christenson, Sherry Fleming, Mark L. Weiss
Summary: Many cell types, including cancer cells, release extracellular vesicles (EVs) that expose tissue factor (TF). It is unclear whether MSC-EVs have a risk of thromboembolism due to TF expression. In this study, the researchers investigated the expression of TF and the procoagulant activity of MSC-EVs. They also examined the impact of different EV isolation methods and cell culture expansion on EV yield, characterization, and potential risk. The results showed that MSC-EVs express TF and have procoagulant activity. Therefore, when using MSC-derived EVs as a therapeutic agent, considerations should be given to TF expression, procoagulant activity, and thromboembolism risk.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Yuan Liu, Christina Holmes
Summary: This review explores the tissue regeneration potential of EVs isolated from bone marrow and adipose tissue, the two most common clinical sources of adult MSCs, focusing on their angiogenic, osteogenic, and immunomodulatory potentials. Other important issues in the development of MSC-derived EV based therapies are also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Fatih Karadeniz, Jung Hwan Oh, Mi Soon Jang, Youngwan Seo, Chang-Suk Kong
Summary: Bone marrow adiposity is a complication in osteoporotic patients, and phytochemicals can alleviate it. This study evaluated the effect of libanoridin on the adipogenic differentiation of bone marrow-derived mesenchymal stromal cells. The results showed that libanoridin inhibited adipogenic differentiation through suppressing MAPK-mediated PPAR gamma signaling. Future studies will further explore the anti-adipogenic effects of libanoridin in vivo and elucidate its action mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Ritu M. Ramamurthy, Martin Rodriguez, Hannah C. Ainsworth, Jordan Shields, Diane Meares, Colin Bishop, Andrew Farland, Carl D. Langefeld, Anthony Atala, Christopher B. Doering, H. Trent Spencer, Christopher D. Porada, Graca Almeida-Porada
Summary: This study investigates the use of CRISPR/Cas9 for gene editing. The results demonstrate that while successful knockin of the RFP reporter gene was achieved, the insertion of the larger FVIII expression cassette was not successful. Additionally, the use of plasmids or introduction of the CRISPR/Cas9 machinery compromised the unique immune-inertness of PLCs.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Maria Yoshida, Ayumu Nakashima, Naoki Ishiuchi, Kisho Miyasako, Keisuke Morimoto, Yoshiki Tanaka, Kensuke Sasaki, Satoshi Maeda, Takao Masaki
Summary: Both adipose-derived and bone marrow-derived mesenchymal stem cells (ADSCs and BMSCs) can suppress renal fibrosis, with ADSCs showing a stronger effect under serum-containing culture conditions. However, ADSC infusion in mice may lead to a higher death rate from pulmonary embolism. Therefore, the selection of ADSCs or BMSCs for clinical applications should consider the target disease and culture method.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell & Tissue Engineering
Caroline Hochheuser, Laurens J. Windt, Nina Y. Kunze, Dieuwke L. de Vos, Godelieve A. M. Tytgat, Carlijn Voermans, Ilse Timmerman
Summary: Neuroblastoma is the second most common solid cancer in childhood, with bone marrow being a common site of distant metastasis. Therapy resistance of residual tumor cells in BM remains a major obstacle for the cure of NB. The role of mesenchymal stromal cells in NB development and progression is not fully understood, with a focus on BM as a metastatic niche.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Cell & Tissue Engineering
Shuang Zhang, Jeroen van de Peppel, Marijke Koedam, Johannes P. T. M. van Leeuwen, Bram C. J. van der Eerden
Summary: The study identifies HSPB7 as a regulator of osteogenic and adipogenic differentiation in bone marrow-derived mesenchymal stromal cells (BMSCs), with a positive role in osteoblastic differentiation and maintaining the balance between osteogenesis and adipogenesis. HSPB7 shows potential as a therapeutic target for bone metabolic diseases.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Engineering, Biomedical
Anamika Singh, Alok Raghav, Parvaiz Ahmad Shiekh, Ashok Kumar
Summary: This study developed a treatment approach for diabetic peripheral neuropathy (DPN) using a fused exosomal system combined with electrical stimulation, showing normalization of nerve conduction velocity and compound muscle action potential, muscle recovery, and observed paracrine effects in controlling hyperglycemia and tissue damage attenuation. It provides a promising effective treatment for DPN and contributes to cutting-edge therapeutic approaches.
BIOACTIVE MATERIALS
(2021)
Article
Pharmacology & Pharmacy
Marta Dymowska, Aleksandra Aksamit, Katarzyna Zielniok, Monika Kniotek, Beata Kaleta, Aleksander Roszczyk, Michal Zych, Filip Dabrowski, Leszek Paczek, Anna Burdzinska
Summary: Although both BM-MSCs and WJ-MSCs have the ability to interact reciprocally with macrophages, the source of MSCs could slightly but significantly modify the response under clinical settings. WJ-MSCs secreted significantly more IL-6 than BM-MSCs and showed higher responsiveness to M1-derived chemotactic signals.
Article
Cell & Tissue Engineering
Jeroen van de Peppel, Gerben J. Schaaf, Adriana Arruda Matos, Yuan Guo, Tanja Strini, Wenda Verschoor, Amel Dudakovic, Andre J. van Wijnen, Johannes P. T. M. van Leeuwen
Summary: This research suggests that the cell surface glycoprotein CD24 could serve as a selective biomarker for identifying subpopulations of BMSCs with varying osteogenic potential. CD24(pos) cells have increased expression of genes related to cell adhesion, motility, and extracellular matrix, while also showing reduced proliferation compared to CD24(neg) cells.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Oncology
Amanpreet Kaur Bains, Lena Behrens Wu, Jennifer Riviere, Sandra Rother, Valentina Magno, Jens Friedrichs, Carsten Werner, Martin Bornhaeuser, Katharina S. Goetze, Michael Cross, Uwe Platzbecker, Manja Wobus
Summary: Myelodysplastic syndromes (MDS) are a heterogeneous group of blood malignancies with specific features and a high risk of progression to acute myeloid leukemia. This study found that MSC-derived extracellular matrix (ECM) plays an important role in MDS, with structural alterations observed in MDS ECM and a potential involvement of glycosaminoglycans (GAGs) in this process.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Bianca Nowlan, Elizabeth D. Williams, Michael Robert Doran
Summary: The study evaluated the impact of partially humanizing mouse bone marrow with human bone marrow-derived stromal cells on human prostate cancer cell behavior. Results showed that PC-3-DsRed cells preferentially populated bones in mice that had received human BMSC, and there were more bone lesions in these mice.
Article
Cell & Tissue Engineering
Behnam Sadeghi, Myrese Witkamp, Dominik Schefberger, Anna Arbman, Olle Ringden
Summary: Human placenta-derived decidua stromal cells (DSCs) have been used in clinical trials for the treatment of acute inflammatory diseases, but the mechanisms of action and interaction with other immune cells require further exploration.
Article
Virology
Caroline Aliane de Souza Prado, Dennyson Leandro M. Fonseca, Youvika Singh, Igor Salerno Filgueiras, Gabriela Crispim Baiocchi, Desiree Rodrigues Placa, Alexandre H. C. Marques, Raquel Costa Silva Dantas-Komatsu, Julia N. Usuda, Paula Paccielli Freire, Ranieri Coelho Salgado, Sarah Maria da Silva Napoleao, Rodrigo Nalio Ramos, Vanderson Rocha, Guangyan Zhou, Rusan Catar, Guido Moll, Niels Olsen Saraiva Camara, Gustavo Cabral de Miranda, Vera Lucia Garcia Calich, Lasse M. Giil, Neha Mishra, Florian Tran, Andre Ducati Luchessi, Helder I. Nakaya, Hans D. Ochs, Igor Jurisica, Lena F. Schimke, Otavio Cabral-Marques
Summary: In this study, the authors analyzed proteome and transcriptome data to investigate the alterations in cell cycle-associated molecules in COVID-19 patients. They found that the expression of cell cycle-associated genes and proteins was changed and correlated with disease severity and trajectories. These findings provide a comprehensive understanding of the distinct alterations in cell cycle regulation in COVID-19 patients and suggest potential therapeutic pathways.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Immunology
Otavio Cabral-Marques, Guido Moll, Rusan Catar, Beate Preuss, Lukas Bankamp, Ann-Christin Pecher, Joerg Henes, Reinhild Klein, A. S. Kamalanathan, Reza Akbarzadeh, Wieke van Oostveen, Bettina Hohberger, Matthias Endres, Bryan Koolmoes, Nivine Levarht, Rudmer Postma, Vincent van Duinen, Anton Jan van Zonneveld, Jeska de Vries-Bouwstra, Cynthia Fehres, Florian Tran, Fernando Yuri Nery do Vale, Kamilla Batista da Silva Souza, Igor Salerno Filgueiras, Lena F. Schimke, Gabriela Crispim Baiocchi, Gustavo Cabral de Miranda, Dennyson Leandro Mathias da Fonseca, Paula Paccielli Freire, Alexander M. Hackel, Hanna Grasshoff, Anja Stahle, Antje Mueller, Ralf Dechend, Xinhua Yu, Frank Petersen, Franziska Sotzny, Thomas P. Sakmar, Hans D. Ochs, Kai Schulze-Forster, Harald Heidecke, Carmen Scheibenbogen, Yehuda Shoenfeld, Gabriela Riemekasten
AUTOIMMUNITY REVIEWS
(2023)
Article
Medicine, General & Internal
Dorota Sikorska, Dorota Kaminska, Rusan Catar, Dashan Wu, Hongfan Zhao, Pinchao Wang, Julian Kamhieh-Milz, Miroslaw Banasik, Mariusz Kusztal, Magdalena Cielecka, Michal Zakliczynski, Rafal Rutkowski, Katarzyna Korybalska, Harald Heidecke, Guido Moll, Wlodzimierz Samborski
Summary: Early identification of allograft vasculopathy and elimination of risk factors is important for improving the prognosis of heart transplant recipients. This pilot study used nailfold video-capillaroscopy to evaluate the microcirculation in heart transplant patients and found that abnormal capillaroscopic patterns were associated with traditional cardiovascular risk factors.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Rheumatology
Bjoern Hegner, Julia Callaghan, Ralf Schindler, Harald Heidecke, Gabriela Riemekasten, Aurelie Philippe, Rusan Catar
Summary: Scleroderma renal crisis is a rare complication of systemic sclerosis characterized by a rapid decline in kidney function. New research suggests that activating autoantibodies targeting specific receptors play a role in the development of scleroderma renal crisis. This study presents a cohort of 10 patients with refractory scleroderma renal crisis who were treated with multimodal therapy, including plasma exchange and vasodilatory interventions. The results showed significant reduction in autoantibody levels and improved kidney function in some patients.
JOURNAL OF SCLERODERMA AND RELATED DISORDERS
(2023)
Article
Hematology
Julian Kamhieh-Milz, Lei Chen, Claudia Goettsch, Anna Maria Pfefferkorn, Anja Hofmann, Coy Brunssen, Gregor M. Mueller, Thomas Walther, Muhammad Imtiaz Ashraf, Guido Moll, Henning Morawietz, Janusz Witowski, Rusan Catar
Summary: This study analyzed the mechanism by which NOX2 contributes to Ang II-induced ET-1 production in human microvascular endothelial cells. The results showed that high-fat diet increased the expression of Ang II and ET-1 in wild-type mice, but not in Nox2-deficient mice. In vitro experiments revealed that Ang II promoted NOX2 expression through induction of the Oct-1 protein and increased production of superoxide anions. Inhibition of Oct-1 and superoxide attenuated Ang II-induced ET-1 production.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Review
Virology
Victor Bastos, Vinicius Pacheco, Erika D. L. Rodrigues, Cassia N. S. Moraes, Adriel L. Nobile, Dennyson Leandro M. Fonseca, Kamilla B. S. Souza, Fernando Y. N. do Vale, Igor S. Filgueiras, Lena F. Schimke, Lasse M. Giil, Guido Moll, Gustavo Cabral-Miranda, Hans D. Ochs, Pedro F. da Costa Vasconcelos, Guilherme D. de Melo, Herve Bourhy, Livia M. N. Casseb, Otavio Cabral-Marques
Summary: Rabies is a neuroinvasive viral disease with a high fatality rate, mainly affecting developing countries in Africa and Asia. It can be prevented through vaccination, but cases still occur in European countries and the United States. Factors such as population growth, increasing income levels, and the COVID-19 pandemic have led to a rise in the animal population, calling for preventive measures, postexposure prophylaxis, and a better understanding of the disease's pathophysiology.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Immunology
Hongfan Zhao, Dashan Wu, Michael Adu Gyamfi, Pinchao Wang, Christian Luecht, Anna Maria Pfefferkorn, Muhammad Imtiaz Ashraf, Julian Kamhieh-Milz, Janusz Witowski, Duska Dragun, Klemens Budde, Ralf Schindler, Daniel Zickler, Guido Moll, Rusan Catar
Summary: Expanded hemodialysis therapy with improved molecular cut-off dialyzers has beneficial effects in reducing uremia-associated chronic systemic microinflammation, which is a driver of endothelial dysfunction and cardiovascular disease in hemodialysis patients. The transcription factor KLF2 and its associated molecular signaling pathways are identified as key targets and regulators in uremia-induced endothelial microinflammation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Julia Nakanishi Usuda, Desiree Rodrigues Placa, Dennyson Leandro M. Fonseca, Alexandre H. C. Marques, Igor Salerno Filgueiras, Victor Gabriel Bastos Chaves, Anny Silva Adri, Amanda Torrentes-Carvalho, Mario Hiroyuki Hirata, Paula Paccielli Freire, Rusan Catar, Gustavo Cabral-Miranda, Lena F. Schimke, Guido Moll, Otavio Cabral-Marques
Summary: This study characterizes the landscape of the interferome signature in DENV infection, highlighting the crucial role of interferome dynamics in the anti-dengue immune response, and identifies potential biomarkers for disease severity.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Crystal C. Uwazie, Bonnie M. Pirlot, Tyler U. Faircloth, Mihir Patel, Rhett N. Parr, Halie M. Zastre, Peiman Hematti, Guido Moll, Devi Rajan, Raghavan Chinnadurai
Summary: Introduces the importance of mesenchymal stromal/stem cells (MSCs) in human physiology and their response to the herbicide Atrazine. Describes the use of the combinatorial assay matrix approach/technology to assess the phenotype and function of MSCs exposed to Atrazine, highlighting its relevance in studying the impact of environmental xenobiotics on human cellular subset involved in regenerative healing responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Henning Morawietz, Heike Brendel, Patrick Diaba-Nuhoho, Rusan Catar, Nikolaos Perakakis, Christian Wolfrum, Stefan R. Bornstein
Summary: Obesity is a major risk factor for cardiovascular and metabolic diseases, and is associated with increased oxidative stress and inflammation. NADPH oxidases play a crucial role in reactive oxygen species generation in the cardiovascular system and metabolically active cells and organs. Imbalance between reactive oxygen species formation and antioxidative capacity contributes to the pathophysiology of cardiovascular and metabolic diseases, with inflammation as a major mechanism. Non-alcoholic fatty liver disease and COVID-19 infections also exhibit increased oxidative stress and inflammation, further increasing the risk of severe clinical complications in individuals with cardiometabolic diseases. This review discusses the role of oxidative stress and inflammation in obesity, cardiovascular diseases, COVID-19 infections, and potential therapeutic strategies.
Article
Geriatrics & Gerontology
Dennyson Leandro M. Fonseca, Igor Salerno Filgueiras, Alexandre H. C. Marques, Elroy Vojdani, Gilad Halpert, Yuri Ostrinski, Gabriela Crispim Baiocchi, Desiree Rodrigues Placa, Paula P. Freire, Shahab Zaki Pour, Guido Moll, Rusan Catar, Yael Bublil Lavi, Jonathan I. Silverberg, Jason Zimmerman, Gustavo Cabral-Miranda, Robson F. Carvalho, Taj Ali Khan, Harald Heidecke, Rodrigo J. S. Dalmolin, Andre Ducati Luchessi, Hans D. Ochs, Lena F. Schimke, Howard Amital, Gabriela Riemekasten, Israel Zyskind, Avi Z. Rosenberg, Aristo Vojdani, Yehuda Shoenfeld, Otavio Cabral-Marques
Summary: Age is a significant risk factor for the severity of COVID-19 due to immunosenescence and age-dependent medical conditions. A cross-sectional study found that the natural production of autoantibodies increases with age and is exacerbated by SARS-CoV-2 infection, particularly in severe COVID-19 patients. Analysis of these autoantibodies revealed an age-dependent stratification of severe COVID-19 patients. Additionally, anti-cardiolipin and anti-platelet glycoprotein autoantibodies were found to significantly increase the likelihood of developing a severe COVID-19 phenotype with aging.
Article
Oncology
Mohammad Hossein Kazemi, Mahdieh Shokrollahi Barough, Zahra Momeni-Varposhti, Alireza Ghanavatinejad, Ali Zarehzadeh Mehrabadi, Behnam Sadeghi, Reza Falak
Summary: Immunotherapy using tumor-infiltrating lymphocytes (TILs) is a promising approach for cancer treatment. Pentoxifylline (PTXF), a xanthine derivative, can limit tumor growth and modify the regulatory-to-cytotoxic TILs ratio and cytokine balance of TILs, promoting anti-tumor responses in a triple-negative breast cancer mouse model.