Review
Pharmacology & Pharmacy
Fengtian Sun, Yuntong Sun, Feng Wu, Wenrong Xu, Hui Qian
Summary: This review introduces the novel cell-free strategy of mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) and their applications in the treatment of diabetes mellitus (DM) and diabetic complications. The major characteristics of MSC-EVs are summarized, and the recent advances in the use of engineered MSC-EVs for treating hyperglycemia-induced tissue damage are discussed. Additionally, the potential applications of engineered MSC-EVs in the therapy of DM-related diseases are presented, and the opportunities and challenges for clinical translation of MSC-EVs, particularly engineered MSC-EVs, are evaluated.
Review
Cell Biology
Qi Zhang, Xin-xing Wan, Xi-min Hu, Wen-juan Zhao, Xiao-xia Ban, Yan-xia Huang, Wei-tao Yan, Kun Xiong
Summary: Stem cell therapies show promising effects in restoring damaged tissue, but multiple types of programmed cell death post-transplantation can compromise their efficiency. Understanding and targeting cell death signaling pathways are crucial for successful stem cell therapies in treating diabetes and related diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Medicine, Research & Experimental
Mengyuan Wang, Ying Li, Hongqiao Wang, Meng Li, Xiaomin Wang, Rongzhen Liu, Daijun Zhang, Wenhua Xu
Summary: Corneal epithelial defects and excessive wound healing can cause severe complications. Stem cells offer a promising solution for regenerating the corneal epithelium and treating severe corneal epithelial injury. Biological scaffolds such as the amniotic membrane, fibrin, and hydrogels provide the necessary signals for stem cell proliferation and differentiation. Researchers have evaluated these scaffolds as potential therapeutic interventions for corneal disorders, identifying both benefits and drawbacks. This study provides a comprehensive overview of the history and use of stem cells in corneal repair, focusing on biological scaffolds used in stem cell transplantation and innovative materials under investigation.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Libo Zhang, Yanhong Li, Riyao Li, Xiaohong Yang, Zimin Zheng, Jingxin Fu, Hai Yu, Xi Chen
Summary: Protein N-glycosylation is a post-translational modification that affects the structure, property, and function of glycoproteins. In vitro N-glycan engineering using recombinant enzymes is an effective strategy to study the functions of specific N-glycans and improve glycoprotein therapeutics.
Article
Neurosciences
Alireza Dodangeh, Elham Hoveizi, Sayed Reza Fatemi Tabatabaei
Summary: Significant progress has been made in treating diabetes using cell therapy derived from stem and progenitor cells. One promising method is the replacement of beta cells through the production of pancreatic beta precursors in cell culture. In this study, endometrial stem cells were isolated and differentiated into insulin-producing cells (IPCs), which were then applied to diabetic mice along with berberine. The findings showed a significant improvement in the treated group compared to control cells, indicating the potential of tissue engineering in diabetes treatment.
MOLECULAR NEUROBIOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Lu Wang, Yiqi Wu, Renqi Yao, Yun Li, Yurou Wei, Yuan Cao, Zicheng Zhang, Mengyao Wu, Hanyu Zhu, Yongming Yao, Hongjun Kang
Summary: Mesenchymal stem cells (MSCs) have been extensively studied for their immunomodulatory properties in inflammatory diseases. Recently, MSC-derived extracellular vesicles (MSC-EVs) have gained attention as a major pathway for intercellular communication. This review summarizes the potential influences of MSC-EVs on different types of programmed cell death and diseases, and discusses the therapeutic effects, current research progress, and challenges of clinical applications of MSC-EVs. Understanding the regulation of inflammation-associated programmed cell death by MSC-EVs is important for identifying molecular mechanisms and modulatory targets.
Article
Engineering, Biomedical
Bin Wang, Wei Liu, Jiao Jiao Li, Senlin Chai, Dan Xing, Hongsheng Yu, Yuanyuan Zhang, Wenjin Yan, Zhihong Xu, Bin Zhao, Yanan Du, Qing Jiang
Summary: In the treatment of osteoarthritis, delivering low doses of MSCs through microcarriers can achieve similar reparative effects as repeated high doses. Mechanistic investigations reveal that 3D growth of MSCs reduces inflammatory factors, enhances early chondrogenesis and extracellular matrix interactions, and maintains the phenotype of co-cultured chondrocytes.
BIOACTIVE MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Ji Sun, Jingbo Li, Zhikun Huan, Stephen J. Pandol, Dechen Liu, Luoran Shang, Ling Li
Summary: Type 1 diabetes mellitus is characterized by autoimmune damage of pancreatic beta cells. This study develops mesenchymal stem cell (MSC)-laden composite beta cell porous microgels (MGs) to address sustainability for the treatment of diabetes. The results demonstrate that the MGs have great potential for the treatment of diabetes providing a promising platform for clinical beta cell transplantation.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Cell Biology
Denise Zujur, Ziadoon Al-Akashi, Anna Nakamura, Chengzhu Zhao, Kazuma Takahashi, Shizuka Aritomi, William Theoputra, Daisuke Kamiya, Koichi Nakayama, Makoto Ikeya
Summary: We developed a stepwise differentiation method to generate matrix-rich cartilage spheroids from induced pluripotent stem cell-derived mesenchymal stem/stromal cells (iMSCs) via the induction of neural crest cells under xeno-free conditions. By using a thienoindazole derivative, TD-198946, we achieved enhanced chondrogenesis in iMSCs. Our findings provide a novel cell source for stem cell-based cartilage repair and offer the potential for biofabrication of larger cartilage tissues using chondrogenic spheroids and technologies such as Kenzan Bioprinting.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Yun-Feng Zhou, Di Zhang, Wan-Ting Yan, Kai Lian, Zheng-Zheng Zhang
Summary: This article reviews the therapeutic potential of multipotent stromal cells (MSCs) in repairing meniscal injuries and discusses the avenues for utilizing these cells. The current progress on biomaterial implants is also reviewed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell & Tissue Engineering
Vincent P. Willard, Holly A. Leddy, Daniel Palmer, Chia-Lung Wu, Wolfgang Liedtke, Farshid Guilak
Summary: The expression and activation of TRPV4 in chondrogenesis play a crucial role in regulating cartilaginous matrix production through modulation of chondrogenic gene markers and Ca2+ signaling. Understanding the involvement of TRPV4 in cartilage development may lead to new therapeutic strategies for cartilage-related diseases and accelerate stem cell differentiation for tissue engineering purposes.
Article
Engineering, Biomedical
Shuangpeng Jiang, Guangzhao Tian, Zhen Yang, Xiang Gao, Fuxin Wang, Juntan Li, Zhuang Tian, Bo Huang, Fu Wei, Xinyu Sang, Liuqi Shao, Jian Zhou, Zhenyong Wang, Shuyun Liu, Xiang Sui, Quanyi Guo, Weimin Guo, Xu Li
Summary: This study confirms that hWJMSC-Exos can enhance the effect of the ACECM scaffold and promote osteochondral regeneration. The promoting effect may be related to the polarization of macrophages and inhibition of inflammatory response by hWJMSC-Exos. Additionally, hWJMSC-Exos contain miRNAs that can promote hyaline cartilage regeneration.
BIOACTIVE MATERIALS
(2021)
Article
Engineering, Biomedical
Guang Yang, Bhushan Mahadik, Ji Young Choi, Justine R. Yu, Trevor Mollot, Bin Jiang, Xiaoming He, John P. Fisher
Summary: The study utilized a microfluidic approach to prepare collagen microbeads loaded with human bone marrow derived mesenchymal stem cells for creating engineered bone grafts. The cell-laden microbeads self-assembled into macro-tissues, enabling precise control over cell spatial distribution at the macroscale, offering flexibility for graft volume and architecture. The approach has the potential to create 3D engineered bone grafts with customized vasculature using modular tissue engineering and 3D printing.
ACTA BIOMATERIALIA
(2021)
Article
Nanoscience & Nanotechnology
Tiep Tien Nguyen, Mai Thi Phuong, Manju Shrestha, Junhyeung Park, Pham Le Minh, Jong Oh Kim, Hyukjae Choi, Joo-Won Nam, Hu-Lin Jiang, Hyung-Sik Kim, Jee-Heon Jeong, Jun-Beom Park, Simmyung Yook
Summary: Potentiation of stem cell potency is crucial for successful bone regeneration in tissue engineering. This study demonstrates a uniform and scalable method to fabricate osteogenic microtissue constructs of mesenchymal stem cell (MSC) spheroids surface-engineered with dexamethasone-releasing polydopamine-coated microparticles (PD-DEXA/MPs). The incorporation of DEXA significantly enhances the osteogenic differentiation of MSC spheroids, and the microparticles remain stable during cell migration. Implantation of these constructs in a mouse model shows substantial bone regeneration.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Medicine, Research & Experimental
Hyun Jik Lee, Chang Woo Chae, Ho Jae Han
Summary: Mesenchymal stem cell (MSC) transplantation has potential for treating diabetes mellitus (DM) and its complications. However, hyperglycemia can cause senescence and dysfunction in transplanted and resident MSCs due to mitochondrial dysfunction. Improving mitochondrial function could enhance the therapeutic efficacy of MSC transplantation in DM.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Biochemistry & Molecular Biology
Marko Anderluh, Francesco Berti, Anna Bzducha-Wrobel, Fabrizio Chiodo, Cinzia Colombo, Federica Compostella, Katarzyna Durlik, Xhenti Ferhati, Rikard Holmdahl, Dragana Jovanovic, Wieslaw Kaca, Luigi Lay, Milena Marinovic-Cincovic, Marco Marradi, Musa Ozil, Laura Polito, Jose Juan Reina, Celso A. Reis, Robert Sackstein, Alba Silipo, Urban Svajger, Ondrej Vanek, Fumiichiro Yamamoto, Barbara Richichi, Sandra J. van Vliet
Summary: Vaccination is a major achievement in preventing infectious diseases, but challenges remain for pathogens with highly variable antigens and in inducing T-cell immunity in cancer. The recent outbreak also highlights the importance of designing smart therapeutic vaccines.
Article
Cell Biology
Himanshu Arora, Rehana Qureshi, Kajal Khodamoradi, Deepa Seetharam, Madhumita Parmar, Derek J. Van Booven, Isabelle Catherine Issa, Robert Sackstein, Dolores Lamb, Joshua M. Hare, Ranjith Ramasamy
Summary: Research has found that a paracrine factor called leptin, released by cells in the testicular microenvironment, plays a key role in regulating the differentiation of testicular stem cells and the production of testosterone by mature Leydig cells. Leptin promotes stem cell differentiation and testosterone production at low doses, but has negative effects at higher doses.
CELL DEATH & DISEASE
(2022)
Article
Immunology
Bijender Kumar, Vahid Afshar-Kharghan, Mayela Mendt, Robert Sackstein, Mark R. R. Tanner, Uday Popat, Jeremy Ramdial, May Daher, Juan Jimenez, Rafet Basar, Luciana Melo Garcia, Mayra Shanley, Mecit Kaplan, Xinhai Wan, Vandana Nandivada, Francia Reyes Silva, Vernikka Woods, April Gilbert, Ricardo Gonzalez-Delgado, Sunil Acharya, Paul Lin, Hind Rafei, Pinaki Prosad Banerjee, Elizabeth J. Shpall
Summary: The current global platelet supply is insufficient, but we have developed a strategy to efficiently produce functional platelets from cord blood. This strategy involves expanding and differentiating megakaryocytes, enhancing platelet production with pharmacological inhibitors, and using gene knockout to evade immune rejection.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell & Tissue Engineering
David Garcia-Bernal, Miguel Blanquer, Carlos M. Martinez, Ana Garcia-Guillen, Ana M. Garcia-Hernandez, M. Carmen Alguero, Rosa Yanez, Maria L. Lamana, Jose M. Moraleda, Robert Sackstein
Summary: Mesenchymal stem/stromal cells (MSCs) have immunoregulatory properties and can be used for the treatment of inflammatory conditions and immune diseases. Through a mouse model, it was found that adipose tissue-derived MSCs (HCELL+ MSCs) can effectively recruit to the affected tissue and exert protective effects on immunopathology, reducing leukocyte infiltration and inflammatory cytokine levels, and improving survival.
NPJ REGENERATIVE MEDICINE
(2022)
Article
Biochemical Research Methods
Kevin Brown Chandler, Daniel E. Marrero Roche, Robert Sackstein
Summary: Bottom-up nLC-MS/MS-based glycoprotein mass spectrometry workflows face challenges due to suppression of hydrophilic glycopeptide ions by more abundant non-glycosylated peptides. This study investigates the use of high-field asymmetric waveform ion mobility spectrometry (FAIMS) for orthogonal separation of non-glycosylated peptides and glycopeptides, and evaluates its impact on glycopeptide assignment confidence. The optimized FAIMS conditions for human alpha-1-acid glycoprotein (AGP) tryptic N-glycopeptide ions transmission and analysis are documented. These optimized methods increase the number of unique glycopeptide identifications and the confidence of glycopeptide assignments.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Meeting Abstract
Hematology
Evan Ales, Robert Sackstein
Review
Multidisciplinary Sciences
Ryan Philip Jajosky, Shang-Chuen Wu, Leon Zheng, Audrey N. Jajosky, Philip G. Jajosky, Cassandra D. Josephson, Marie A. Hollenhorst, Robert Sackstein, Richard D. Cummings, Connie M. Arthur, Sean R. Stowell
Summary: Enzymes are important in catalyzing biochemical reactions and have significant impacts on human health and disease. Variants and deficiencies in enzymes can lead to changes in the expression of glycans, which can affect physiology, disease susceptibility, and disease development. Enzyme deficiencies related to carbohydrate blood groups have important implications in clinical medicine, such as infection risk, bleeding risk, transfusion medicine, and organ transplantation. This review explores the enzymes responsible for the biosynthesis of carbohydrate-based blood group antigens, their expression in the human population, and the evolutionary selective pressures that may contribute to the variation in carbohydrate structures and its implications for human disease.
Article
Biochemical Research Methods
Kevin Brown Chandler, Carlos Humberto Pavan, Hannah G. Cotto Aparicio, Robert Sackstein
Summary: Mucin-domain glycoproteins expressed on cancer cell surfaces have important functions in cell adhesion, cancer progression, stem cell renewal, and immune evasion. However, our understanding of the composition of these mucin-domain glycoproteins in head and neck squamous cell carcinoma (HNSCC) is limited. In this study, a workflow involving enzyme capture and characterization techniques was employed to identify and analyze mucin-domain glycoproteins in HNSCC. The results provide insights into the diversity and unique expression patterns of mucin-domain glycoproteins in different HNSCC cell lines, which could contribute to a better understanding of aggressive tumor cell phenotypes. Data associated with this study have been deposited in a public repository for further analysis.
JOURNAL OF PROTEOME RESEARCH
(2023)
Meeting Abstract
Oncology
Evan Ales, Robert Sackstein
Article
Biochemical Research Methods
Kevin Brown Chandler, Carlos Humberto Pavan, Hannah G. Cotto Aparicio, Robert Sackstein
Summary: Mucin-domain glycoproteins are crucial in the pathobiology of head and neck squamous cell carcinoma (HNSCC), yet our understanding of the composition of the mucinome in HNSCC is incomplete. In this study, we used an inactive mutant enzyme (StcEE447D) to capture and characterize mucin-domain glycoproteins in HNSCC cell line lysates. We identified common mucin-domain glycoproteins in multiple HNSCC cell lines and unique mucin-domain glycoproteins in HSC-3 cells, derived from a highly aggressive metastatic tongue squamous cell carcinoma. This study provides a foundation for a more comprehensive characterization of mucinome components in HNSCC.
JOURNAL OF PROTEOME RESEARCH
(2023)
Meeting Abstract
Oncology
Kevin Brown Chandler, Brittany Montesino, Nan Hu, Juan M. Lozano, Robert Sackstein
Meeting Abstract
Biochemistry & Molecular Biology
Kevin Brown Chandler, Hani Samarah, Agata Steenackers, Robert Sackstein
Article
Biochemistry & Molecular Biology
Brittany Montesino, Agata Steenackers, Juan M. Lozano, Geoffrey D. Young, Nan Hu, Robert Sackstein, Kevin Brown Chandler
Summary: Head and neck cancer is the seventh most common cancer in the world. Fucosylated carbohydrate antigens play a prominent role in tumor cell adhesion and metastasis. In this study, researchers investigated the functional role of fucose-modified glycoproteins in head and neck cancer pathobiology. They found that inactivating polymorphisms of the fut2 gene were associated with an increased incidence of head and neck cancer. Moreover, they observed the presence of alpha 1,2-fucosylated Lewis Y epitope in cancer tumors. This knowledge will serve as the foundation for future studies on the role of fucosylated glycoproteins in head and neck cancer.
Meeting Abstract
Oncology
B. Montesino, N. Hu, J. M. Lozano, R. Sackstein, K. B. Chandler
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2022)
Meeting Abstract
Cell & Tissue Engineering
D. G. Bernal, S. Muntion, S. Preciado, J. I. Gil-Chinchilla, R. Sackstein, F. Sanchez-Guijo, J. Moraleda