期刊
NEW BIOTECHNOLOGY
卷 28, 期 3, 页码 211-217出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nbt.2010.08.009
关键词
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Genomic information becomes useful knowledge only when the structures and functions of gene products are understood. In spite of a vast array of analytical tools developed for biological studies in recent years, producing proteins at will is still a bottleneck in post-genomic studies. The cell-free protein production system we developed using wheat embryos has enabled us to produce high quality proteins for genome-wide functional and structural analyses and at the same time circumvent almost all the limitations, such as biohazards and costs, that have hampered conventional cell-free protein synthesis systems. In the present article, we introduce examples of our new wheat germ cell-free protein production system and its application to functional and structural analyses, with the focus on the former.
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