期刊
STEM CELLS
卷 33, 期 10, 页码 3152-3157出版社
WILEY
DOI: 10.1002/stem.2106
关键词
Adult hematopoietic stem cells; Differentiation; Hematopoiesis; Megakaryocyte; Stem cell plasticity
资金
- Swiss National Science Foundation Singergia Grant [CRSII3_147684]
- ERC Grant [StG_311422]
- SystemsX.ch RTD project StoNets
- EU framework 7 HEALTH research program PluriMes
- EU FP7 Grant BIODESIGN [FP7-NMP-2010-LARGE-4]
- Swiss National Science Foundation (SNF) [CRSII3_147684] Funding Source: Swiss National Science Foundation (SNF)
Despite increasingly stringent methods to isolate hematopoietic stem cells (HSCs), considerable heterogeneity remains in terms of their long-term self-renewal and differentiation potential. Recently, the existence of long-lived, self-renewing, myeloid-restricted progenitors in the phenotypically defined HSC compartment has been revealed, but these cells remain poorly characterized. Here, we used an in vitro single-cell analysis approach to track the fate of 330 long-term HSCs (LT-HSC; Lin- cKit+ Sca-1+ CD150+ CD48- CD34-) cultured for 5 days under serum-free basal conditions. Our analysis revealed a highly heterogeneous behavior with approximately 15% of all phenotypic LT-HSCs giving rise to megakaryocytes (Mk). Surprisingly, in 65% of these cases, Mk development occurred in the absence of cell division. This observation suggests that myeloid-restricted progenitors may not derive directly from LT-HSCs but instead could share an identical cell surface marker repertoire.
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