期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 581, 期 -, 页码 78-85出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2015.04.006
关键词
Cryo-electron tomography; Cryo-focused-ion beam; Direct electron detector; Phase plate
资金
- European Research Council (ERC) [243047 INCEL]
- Swiss National Science Foundation [SNSF 31003A_159706/1]
- Forschungskredit of the University of Zurich [FK-13-049]
Structural analysis of macromolecular assemblies and their remodeling during physiological processes is instrumental to defining the fundament of cellular and molecular biology. Recent advances in computational and analytical tools for cryo-electron tomography have enabled the study of macromolecular structures in their native environment, providing unprecedented insights into cell function. Moreover, the recent implementation of direct electron detectors has progressed cryo-electron tomography to a stage where it can now be applied to the reconstruction of macromolecular structures at high resolutions. Here, we discuss some of the recent technical developments in cryo-electron tomography to reveal structures of macromolecular complexes in their physiological medium, focusing mainly on eukaryotic cells. (C) 2015 Elsevier Inc. All rights reserved.
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