Article
Multidisciplinary Sciences
Yuko Hara, Yoshitaka Mizobe, Yukiko U. Inoue, Yasumasa Hashimoto, Norio Motohashi, Yoshiaki Masaki, Kohji Seio, Shin'ichi Takeda, Tetsuya Nagata, Matthew J. A. Wood, Takayoshi Inoue, Yoshitsugu Aoki
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Nariko Arimura, Mako Okada, Shinichiro Taya, Ken-ichi Dewa, Akiko Tsuzuki, Hirotomo Uetake, Satoshi Miyashita, Koichi Hashizume, Kazumi Shimaoka, Saki Egusa, Tomoki Nishioka, Yuchio Yanagawa, Kazuhiro Yamakawa, Yukiko U. Inoue, Takayoshi Inoue, Kozo Kaibuchi, Mikio Hoshino
Article
Biology
Kou Hiraga, Yukiko U. Inoue, Junko Asami, Mayuko Hotta, Yuki Morimoto, Shoji Tatsumoto, Mikio Hoshino, Yasuhiro Go, Takayoshi Inoue
COMMUNICATIONS BIOLOGY
(2020)
Article
Cell Biology
Yukiko U. Inoue, Yuki Morimoto, Mayumi Yamada, Ryosuke Kaneko, Kazumi Shimaoka, Shinji Oki, Mayuko Hotta, Junko Asami, Eriko Koike, Kei Hori, Mikio Hoshino, Itaru Imayoshi, Takayoshi Inoue
Summary: Fluorescent reporter mouse lines and Cre/Flp recombinase driver lines are essential for investigating molecular functions in vivo. The study reverified and optimized the phospho-PCR method to obtain highly pure long ssDNAs for knock-in mouse generation, enabling successful generation of knock-in mice through microinjection and electroporation methods, thus broadening its applicability.
Article
Anatomy & Morphology
Ryuichi Kimura, Yukiko U. Inoue, Takako Kikkawa, Misako Tatehana, Yuki Morimoto, Hitoshi Inada, Shinya Oki, Takayoshi Inoue, Noriko Osumi
Summary: The study confirmed the expression and localization of REST in the early stages of spermatogenesis, specifically in spermatogonia and Sertoli cells. This suggests that REST may modulate the epigenetic state of male germline cells, with implications for neurodevelopmental and age-related disorders. The knock-in mouse model generated in this study could be valuable for exploring REST-associated molecular mechanisms further.
DEVELOPMENTAL DYNAMICS
(2022)
Article
Multidisciplinary Sciences
Toshiko Yamazawa, Takuya Kobayashi, Nagomi Kurebayashi, Masato Konishi, Satoru Noguchi, Takayoshi Inoue, Yukiko U. Inoue, Ichizo Nishino, Shuichi Mori, Hiroto Iinuma, Noriaki Manaka, Hiroyuki Kagechika, Arkady Uryash, Jose Adams, Jose R. Lopez, Xiaochen Liu, Christine Diggle, Paul D. Allen, Sho Kakizawa, Keigo Ikeda, Bangzhong Lin, Yui Ikemi, Kazuto Nunomura, Shinsaku Nakagawa, Takashi Sakurai, Takashi Murayama
Summary: The study shows that an oxolinic acid-derivative RyR1 inhibitor can effectively prevent and treat various malignant hyperthermia (MH) and heat stroke mouse models.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Yuko Nitahara-Kasahara, Shuji Mizumoto, Yukiko U. Inoue, Shota Saka, Guillermo Posadas-Herrera, Aki Nakamura-Takahashi, Yuki Takahashi, Ayana Hashimoto, Kohei Konishi, Shinji Miyata, Chiaki Masuda, Emi Matsumoto, Yasunobu Maruoka, Takahiro Yoshizawa, Toshiki Tanase, Takayoshi Inoue, Shuhei Yamada, Yoshihiro Nomura, Shin'ichi Takeda, Atsushi Watanabe, Tomoki Kosho, Takashi Okada
Summary: Researchers generated mouse models for musculocontractural Ehlers-Danlos syndrome (mcEDS) through CRISPR/Cas9 genome editing, revealing pathophysiological features such as growth impairment, skin fragility, and muscle-related phenotypes caused by depletion of dermatan sulfate (DS). This study provides insights into the pathophysiology of mcEDS and may contribute to the development of novel treatment strategies.
DISEASE MODELS & MECHANISMS
(2021)
Article
Cell Biology
Yuko Nitahara-Kasahara, Guillermo Posadas-Herrera, Shuji Mizumoto, Aki Nakamura-Takahashi, Yukiko U. Inoue, Takayoshi Inoue, Yoshihiro Nomura, Shin'ichi Takeda, Shuhei Yamada, Tomoki Kosho, Takashi Okada
Summary: Deficiency of dermatan sulfate in mcEDS causes pathological distribution and functional abnormalities of decorin in the skeletal muscle, leading to disturbances in muscle myogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Neurosciences
Yukiko U. Inoue, Hideki Miwa, Kei Hori, Ryosuke Kaneko, Yuki Morimoto, Eriko Koike, Junko Asami, Satoshi Kamijo, Mitsuhiko Yamada, Mikio Hoshino, Takayoshi Inoue
Summary: This study utilized advanced genome editing techniques to generate a series of knock-in mouse lines, preserving the endogenous transcriptional regulations of the Oxtr gene. By using epitope tagging strategy and specific antibodies, the distribution of Oxtr protein on the neural membrane was visualized for the first time. Additionally, the expression of TdTomato reporter gene, Cre recombinase, and inducible Cre-ERT2 was successfully induced in Oxtr-expressing neurons. These tools provide valuable resources for future functional studies in Oxt-responsive neurons.
Article
Multidisciplinary Sciences
Kotaro Oyama, Vadim Zeeb, Toshiko Yamazawa, Nagomi Kurebayashi, Fuyu Kobirumaki-Shimozawa, Takashi Murayama, Hideto Oyamada, Satoru Noguchi, Takayoshi Inoue, Yukiko U. Inoue, Ichizo Nishino, Yoshie Harada, Norio Fukuda, Shin'ichi Ishiwata, Madoka Suzuki
Summary: This study investigates the relationship between mutant Ryanodine receptor type 1 (RyR1) and malignant hyperthermia (MH) using an optically controlled local heat-pulse method. The results show that RyR1 mutants are more heat sensitive and induce intracellular calcium bursts through heat-induced calcium release (HICR). HICR is also observed in skeletal muscles. This study highlights the complexity of MH and the positive feedback of HICR in thermogenesis in MH patients.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Daiki X. Sato, Yukiko U. Inoue, Nahoko Kuga, Satoko Hattori, Kensaku Nomoto, Yuki Morimoto, Giovanni Sala, Hideo Hagihara, Takefumi Kikusui, Takuya Sasaki, Yuji Ikegaya, Tsuyoshi Miyakawa, Takayoshi Inoue, Masakado Kawata
Summary: This study introduces human-specific mutations into mice and investigates their impact on behavior, neurophysiology, and molecular level. The results show that the introduction of the Ile mutation leads to reduced anxiety-related traits and altered oscillatory activity in the amygdala under anxiogenic conditions. Transcriptome analysis confirms changes in gene expression in the amygdala, further supporting the observed phenotypes.
Article
Physiology
Yoshitaka Tsuboi, Kotaro Oyama, Fuyu Kobirumaki-Shimozawa, Takashi Murayama, Nagomi Kurebayashi, Toshiaki Tachibana, Yoshinobu Manome, Emi Kikuchi, Satoru Noguchi, Takayoshi Inoue, Yukiko U. Inoue, Ichizo Nishino, Shuichi Mori, Ryosuke Ishida, Hiroyuki Kagechika, Madoka Suzuki, Norio Fukuda, Toshiko Yamazawa
Summary: The R2509C mutation in RYR1 causes dysfunctional calcium dynamics in a mutant-gene dose-dependent manner in skeletal muscles, leading to MH-like episodes in heterozygous mice and embryonic lethality in homozygous mice.
JOURNAL OF GENERAL PHYSIOLOGY
(2022)