期刊
NEUROSCIENCE LETTERS
卷 523, 期 2, 页码 180-185出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2012.06.072
关键词
Oligodendrocyte progenitor cells; Mouse embryonic stem cells; Neural differentiation; Type-1 and type-2 astrocytes
资金
- Shriners Hospitals for Children
- National Institutes of Health [R01NS061983, R01ES015988]
- National Multiple Sclerosis Society
Oligodendrocyte progenitor cells (OPCs) in primary culture can give rise to mature oligodendrocytes and type-2, but not type-1 astrocytes depending on the culture conditions. The OPCs thus are called oligodendrocyte-type-2 astrocyte (O2-A) progenitor cells. Mouse embryonic stem cells (mESCs) have been efficiently differentiated into OPCs; however, the fate plasticity of mESC-derived OPCs is not well characterized. In the present study, using GFP-Olig2 mESC line, we showed that the Olig2(+)/GFP(+)/A2B5(+)/NG2(+) OPCs derived from GFP-Olig2 mESCs can mature into oligodendrocytes when co-cultured with mESC-derived neurons. Interestingly, when induced to astrocytic differentiation by bone morphogenetic protein-4, these mESC-derived OPCs can not only generate type-2 astrocytes, but also type-1 astrocytes. These results challenge the dogma that OPCs in culture can only generate type-2, but not type-1 astrocytes, and support the in vivo finding that during perinatal development, OPCs can give rise to a subset of type-1 astrocytes. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
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