期刊
NEUROSCIENCE LETTERS
卷 530, 期 2, 页码 121-126出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2012.10.011
关键词
Fear extinction; Lateral amygdala; GluN2B-containing NMDA receptor; Ifenprodil; Ro25-6981
资金
- Original Technology Research Program for Brain Science through the National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2011-0019226]
- National Research Foundation of Korea (NRF)
- Korea government (MEST) [2011-0018209]
- Korea Ministry of Education
- National Research Foundation of Korea [2011-0018209, 2011-0019226, 과06A1204] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
We have previously characterized the ex vivo depotentiation (depotentiation,,No) of conditioning-induced synaptic potentiation at thalamic input synapses onto the lateral amygdala (T-LA synapses) as a potential cellular substrate for fear extinction: both depotentiation(ex vivo) and fear extinction require NMDA receptors, mitogen-activated protein kinases, metabotropic glutamate receptor 1, de novo protein synthesis and AMPA receptor internalization in the amygdala. Surprisingly, as shown in our and other previous studies, ifenprodil, an antagonist of GluN2B-containing NMDA receptors, fails to inhibit depotentiation(ex vivo) at a saturating concentration (10 mu M), although it has been suggested that GluN2B-containing NMDA receptors are required for fear extinction. Because ifenprodil is also known to act on other molecular targets in addition to GluN2B-containing NMDA receptors, especially at high concentrations (i.e., >= 10 mu M), the ineffectiveness of 10 mu M of ifenprodil may be due to its side effects. Therefore, in the present study, we tested Ro25-6981, a more specific antagonist of GluN2B-containing NMDA receptors, and a lower concentration (3 mu M) of ifenprodil, which may reduce any possible side effects. Ro25-6981 (3 mu M) blocked both depotentiation(ex vivo) and late-phase long-term potentiation at T-LA synapses. While 10 mu M ifenprodil failed to inhibit depotentiation(ex vivo), a lower concentration (3 mu M) of ifenprodil blocked depotentiation(ex vivo). Together, our findings suggest that depotentiation(ex vivo) requires GluN2B-containing NMDA receptors. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
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