期刊
NEUROSCIENCE LETTERS
卷 457, 期 2, 页码 75-79出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2009.03.021
关键词
NR4A2; Parkinsonism; Whole genome expression analysis; Allele specific expression analysis
资金
- Parkinson's Disease Society UK
- Brain Research Trust
- MRC [G0200373] Funding Source: UKRI
- Medical Research Council [G0200373] Funding Source: researchfish
Objective: We performed a mutation screen of NR4A2 (also known as NURR1) in 409 Parkinson's disease (PD) patients. We identified a novel single base substitution in the 5'UTR of the NR4A2 (also known as NURR1) gene (c.-309C > T). Results: We have performed expression studies in neuronal cell lines showing that the c.-309C > T mutation reduces NR4A2 mRNA expression in vitro. We have confirmed this finding in vivo by performing allele specific real-time PCR from brain tissue harbouring the 309C > T mutation and show a 3.48 +/- 1.62 fold reduction in mRNA expression of the mutant allele compared to wild-type. In addition we have undertaken genome wide expression analysis of the mutant NR4A2 brain and shown underexpressed genes were significantly enriched for gene ontology categories in nervous system development and synaptic transmission and overexpressed genes were enriched for unfolded protein response and morphogenesis. Lastly we have shown that the c.-309C > T mutation abrogates the protective effect of wild-type NR4A2 against apoptopic stress. Conclusions: Our findings indicate the c.-309C > T mutation reduces NR4A2 expression resulting in the downregulation of genes involved in the development and maintenance of the nervous system and synaptic transmission. These downregulated pathways contained genes known to be transactivated by NR4A2 and were not disrupted in idiopathic PD brain suggesting causality of the mutation. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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