期刊
NEUROSCIENCE LETTERS
卷 465, 期 1, 页码 36-40出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2009.09.001
关键词
Intra-bone marrow-bone marrow transplantation; SAMP8; Water maze; Escape latency
资金
- Ministry of Education, Culture, Sports, Science and Technology
- Ministry of Education, Health and Labor Sciences Research Grants
- Department of Transplantation for Regeneration Therapy
- Molecular Medical Science Institute
- Otsuka Pharmaceutical Co., Ltd
- Japan Immunoresearch Laboratories Co., Ltd. (JIMRO)
Bone marrow cells (BMCs) can increase the number of activated microglias, which play a central role in the inflammatory response in Alzheimer's disease (AD). Senescence-accelerated mouse (SAM) prone 8 (SAMP8) are widely used in various experiments because of cognitive deficits observed with age. In the present study, 4-month-old SAMP8 were reconstituted with BMCs of C57BL/6 mice by intra-bone marrow-bone marrow transplantation (IBM-BMT), which can reconstitute both donor-derived hemopoietic stem cells and mesenchymal stem cells. Three months after IBM-BMT, the impairment of spatial memory in SAMP8 was found to be ameliorated after analyzing the results of the water maze test. Although IL-1 beta, IL-6 and iNOS increased and TGF-beta decreased in 7 M SAMP8, IL-1 beta, IL-6 and iNOS decreased while TGF-beta increased after IBM-BMT by RT-PCR. Moreover, oxidative stress-related heme oxygenase-1 (HO-1) increased in 7 M SAMP8, but significantly decreased after IBM-BMT. In conclusion, this is the first report suggesting that the impaired cognitive ability of SAMP8 is ameliorated by IBM-BMT. It seems likely that decreases in IL-1 beta, IL-6, iNOS and HO-1 are a result of the development of donor-derived BMCs. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据