期刊
NEUROSCIENCE LETTERS
卷 447, 期 1, 页码 62-67出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2008.09.051
关键词
Hippocampal slice; Actin; Semliki Forest virus; RhoA; Pyramidal neuron; Spines
资金
- Canadian Institute for Health Research
The Rho family of small GTPase proteins are involved in the formation and maintenance of neuronal dendrites. In this study, we show that Daam1, a member of the Diaphanous-related formin protein family and a downstream effector for RhoA, is localized to the dendrites of hippocampal neurons. Immunoblot analysis showed that Daam1 is enriched in the mouse hippocampus and co-fractionates in brain lysates with dendritic and synaptic proteins. Immunohistochemical analysis revealed that Daam1 protein distributes in a punctate pattern throughout the cell body and dendritic shafts of dissociated hippocampal neurons and organotypic hippocampal cultures. Although Daam1 is mostly expressed in the shaft of dendrites, co-stainings with SV2 or PSD95 revealed that Daam1 is also present at some synapses. In addition, viral directed expression of a fluorescently tagged Daam1 fusion protein in hippocampal slices resulted in targeted delivery to the dendrites of pyramidal neurons, leading to a reduction in the density of spines. (c) 2008 Elsevier Ireland Ltd. All rights reserved.
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