Article
Endocrinology & Metabolism
Patrizia D'Adamo, Anemari Horvat, Antonia Gurgone, Maria Lidia Mignogna, Veronica Bianchi, Michela Masetti, Maddalena Ripamonti, Stefano Taverna, Jelena Velebit, Maja Malnar, Marko Muhic, Katja Fink, Angela Bachi, Umberto Restuccia, Sara Belloli, Rosa Maria Moresco, Alessia Mercalli, Lorenzo Piemonti, Maja Potokar, Sasa Trkov Bobnar, Marko Kreft, Helena H. Chowdhury, Matjaz Stenovec, Nina Vardjan, Robert Zorec
Summary: Proteomic analysis and glucose uptake studies in Gdi1 knockout mice reveal significant changes in astrocyte-resident glycolytic enzymes and increased glucose uptake, leading to cognitive impairment. A selective impairment in working memory was observed in mice with Gdi1 deletion restricted to astrocytes, which was rescued by inhibiting glycolysis. These findings suggest a new astrocyte-based mechanism in neurodevelopmental disorders and potential therapeutic opportunities targeting aerobic glycolysis.
METABOLISM-CLINICAL AND EXPERIMENTAL
(2021)
Review
Plant Sciences
Yutaro Shimizu, Tomohiro Uemura
Summary: Membrane trafficking is crucial for organelle maintenance and function, and the TGN plays a critical role as a sorting station. Recent studies have identified distinct subdomains of the TGN for different trafficking pathways.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Fan-Chen Huang, Shin-Fei Chi, Pei-Ru Chien, Yin-Tzu Liu, Hsin-Nung Chang, Choun-Sea Lin, Hau-Hsuan Hwang
Summary: AtRAB8s interact with AtRTNLB proteins and are involved in Agrobacterium tumefaciens and Pseudomonas syringae infection processes.
PLANT AND CELL PHYSIOLOGY
(2021)
Review
Medicine, Research & Experimental
Jiayi Liu, Xuanjun Zheng, Xiaoqian Wu
Summary: This review summarizes the importance of Rab GTPase in cardiovascular diseases and suggests its potential as a therapeutic target.
Article
Biochemical Research Methods
Xiao X. Zhang, John William Young, Leonard J. Foster, Franck Duong
Summary: Soluble proteins often interact with membranes to perform important functions, but characterizing these interactions can be challenging due to their transient nature and the poor solubility of phospholipids. In this study, nanodiscs and quantitative proteomics were used to identify lipid-binding proteins in yeast, revealing key players in vesicle trafficking and a member of the Hsp40/J-protein family with specificity for phosphatidic acid. This research showcases the utility of combining proteomics with lipid nanodiscs to uncover unique protein-lipid interactions.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Cell Biology
Aparajita Lahree, Sara de Jesus Santos Baptista, Sofia Marques, Veronika Perschin, Vanessa Zuzarte-Luis, Manisha Goel, Hadi Hasan Choudhary, Satish Mishra, Christian Stigloher, Marino Zerial, Varadharajan Sundaramurthy, Maria M. Mota
Summary: Intracellular pathogens manipulate host cells to survive and thrive. This study shows that liver-stage Plasmodium parasites compete with the host to sequester a host endosomal-adaptor protein (APPL1) involved in regulating endocytosis signaling. There is a correlation between the level of APPL1 enrichment at the parasitophorous vacuole membrane (PVM) and parasite size during exoerythrocytic development.
Article
Multidisciplinary Sciences
Xin Yong, Guowen Jia, Zhe Liu, Chunzhuang Zhou, Jiamin Yi, Li Chen, Lu Chen, Yuan Wang, Qingxiang Sun, Daniel D. Billadeau, Zhaoming Su, Da Jia
Summary: In this study, the structure of the Drosophila Mon1-Ccz1-RMC1 complex was determined using cryogenic-electron microscopy. RMC1 acts as a scaffolding subunit and binds to both Mon1 and Ccz1, explaining the binding specificity of the complex. The assembly of RMC1 with Mon1-Ccz1 is required for cellular RAB7A activation, autophagic functions, and organismal development in zebrafish.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Genetics & Heredity
Margaret F. Lippincott, Wanxue Xu, Abigail A. Smith, Xinyu Miao, Agathe Lafont, Omar Shennib, Gordon J. Farley, Riwa Sabbagh, Angela Delaney, Maria Stamou, Lacey Plummer, Kathryn Salnikov, Neoklis A. Georgopoulos, Veronica Mericq, Richard Quinton, Frederic Tran Mau-Them, Sophie Nambot, Asma Hamad, Helen Brittain, Rebecca S. Tooze, Eduardo Calpena, Andrew O. M. Wilkie, Marjolaine Willems, William F. Crowley, Ravikumar Balasubramanian, Nathalie Lamarche-Vane, Erica E. Davis, Stephanie B. Seminara
Summary: This study identified ARHGAP35 as a new autosomal dominant genetic driver for idiopathic hypogonadotropic hypogonadism (IHH) and ARHGAP5 as a candidate gene for IHH. These observations suggest a novel role for the p190 RhoGAP proteins in GnRH neuronal development and integrity.
GENETICS IN MEDICINE
(2022)
Article
Cell Biology
Yuan-Jing Zou, Meng-Meng Shan, Hong-Hui Wang, Zhen-Nan Pan, Meng-Hao Pan, Yi Xu, Jia-Qian Ju, Shao-Chen Sun
Summary: Our study investigated the roles of RAB14 during oocyte meiotic maturation, showing that depletion of RAB14 caused large polar bodies and defects in spindle migration. The regulation on actin-based spindle migration and Golgi apparatus distribution by RAB14 during mouse oocyte meiotic maturation was suggested by our results.
CELL PROLIFERATION
(2021)
Article
Gastroenterology & Hepatology
Olga A. Mareninova, Dustin L. Dillon, Carli J. M. Wightman, Iskandar Yakubov, Toshimasa Takahashi, Herbert Y. Gaisano, Keith Munson, Masaki Ohmuraya, David Dawson, Ilya Gukovsky, Anna S. Gukovskaya
Summary: The study reveals the significant role of Rab9 in regulating autophagy in pancreatic diseases. Overexpression of Rab9 stimulates noncanonical autophagy while inhibiting canonical autophagy, exacerbating pancreatitis.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Qingchun Lu, Po-Shun Wang, Ling Yang
Summary: This review focuses on highlighting Golgi-associated Rab GTPases as master regulators of autophagic flux, discussing the future research prospects in elucidating their role and mechanism in autophagy and autophagy-related diseases.
CELL AND BIOSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Alazne Arrazola Sastre, Miriam Luque Montoro, Hadriano M. Lacerda, Francisco Llavero, Jose L. Zugaza
Summary: Rab and Arf GTPases play crucial roles in the pathogenesis and progression of neurodegenerative diseases, making them potential therapeutic targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Plant Sciences
R. Ofoe
Summary: Heterotrimeric G-proteins regulate important signaling pathways for growth and development in both plants and animals. Understanding the complexities of plant G-proteins could potentially impact global food security by improving crop development. Further research on molecular mechanisms underlying G-protein signaling in diverse plant species will be essential for enhancing our understanding and improving crop improvement.
Review
Medicine, Research & Experimental
Juan Carlos Martinez-Morales, K. Helivier Solis, M. Teresa Romero-Avila, Guadalupe Reyes-Cruz, J. Adolfo Garcia-Sainz
Summary: G protein-coupled receptors (GPCRs) are membrane proteins that function as sensors and play significant roles in various physiological and pathological processes. This review provides an overview of the current understanding of the structure, signaling, internalization, and recycling of GPCRs.
ARCHIVES OF MEDICAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Yuriko Sobu, Paulina S. Wawro, Herschel S. Dhekne, Wondwossen M. Yeshaw, Suzanne R. Pfeffer
Summary: Mutations that activate LRRK2 protein kinase lead to Parkinson's disease and hinder cilia formation. LRRK2 kinase activity decreases the probability of ciliation and increases the percentage of cilia lost upon serum addition. Rab10 and RILPL1 proteins play crucial roles in these processes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Neurosciences
Oladiran I. Olateju, Lorenzo More, J. Simon C. Arthur, Bruno G. Frenguelli
Summary: Neurogenesis in the adult hippocampus can be stimulated by exposure to an enriched environment, leading to increased production of neurons and benefits for health and cognition. MSK1 plays a negative regulatory role post-enrichment by influencing the number of cells destined to become neurons, thereby potentially serving as a homeostatic control on the integration of new neurons into the dentate gyrus.
Article
Endocrinology & Metabolism
Patrizia D'Adamo, Anemari Horvat, Antonia Gurgone, Maria Lidia Mignogna, Veronica Bianchi, Michela Masetti, Maddalena Ripamonti, Stefano Taverna, Jelena Velebit, Maja Malnar, Marko Muhic, Katja Fink, Angela Bachi, Umberto Restuccia, Sara Belloli, Rosa Maria Moresco, Alessia Mercalli, Lorenzo Piemonti, Maja Potokar, Sasa Trkov Bobnar, Marko Kreft, Helena H. Chowdhury, Matjaz Stenovec, Nina Vardjan, Robert Zorec
Summary: Proteomic analysis and glucose uptake studies in Gdi1 knockout mice reveal significant changes in astrocyte-resident glycolytic enzymes and increased glucose uptake, leading to cognitive impairment. A selective impairment in working memory was observed in mice with Gdi1 deletion restricted to astrocytes, which was rescued by inhibiting glycolysis. These findings suggest a new astrocyte-based mechanism in neurodevelopmental disorders and potential therapeutic opportunities targeting aerobic glycolysis.
METABOLISM-CLINICAL AND EXPERIMENTAL
(2021)
Article
Biochemistry & Molecular Biology
Maria Lidia Mignogna, Stefano Musardo, Giulia Ranieri, Susanna Gelmini, Pedro Espinosa, Paolo Marra, Sara Belloli, Valentina Murtaj, Rosa Maria Moresco, Camilla Bellone, Patrizia D'Adamo
Summary: Mutations in the RAB39B gene cause X-linked intellectual disability, often comorbid with autism spectrum disorders or early Parkinson's disease. Lack of RAB39B affects neuronal dendritic spine refinement, leading to a more excitable synaptic network correlated with immature spine arrangement and behavioral/cognitive alterations in adult mice. Targeting Ca2+-permeable AMPARs may provide future pharmaceutical interventions for RAB39B-associated conditions.
MOLECULAR PSYCHIATRY
(2021)
Article
Biochemistry & Molecular Biology
Maria Lidia Mignogna, Romina Ficarella, Susanna Gelmini, Lucia Marzulli, Emanuela Ponzi, Alessandra Gabellone, Antonia Peschechera, Massino Alessio, Lucia Margari, Mattia Gentile, Patrizia D'Adamo
Summary: A novel RAB39B nonstop mutation was found in a family with clinical features including ID, ASD, and poor motor coordination. The mutation led to RAB39B protein instability and downregulation, impacting AMPAR trafficking and dendritic spine morphology. Experimental results showed that behavioral performance was affected in a disease-specific manner in Rab39b knockdown mice with impaired social behavior and memory functions.
HUMAN MOLECULAR GENETICS
(2022)
Article
Immunology
Michela Masetti, Roberta Carriero, Federica Portale, Giulia Marelli, Nicolo Morina, Marta Pandini, Marta Iovino, Bianca Partini, Marco Erreni, Andrea Ponzetta, Elena Magrini, Piergiuseppe Colombo, Grazia Elefante, Federico Simone Colombo, Joke M. M. den Haan, Clelia Peano, Javier Cibella, Alberto Termanini, Paolo Kunderfranco, Jolanda Brummelman, Matthew Wai Heng Chung, Massimo Lazzeri, Rodolfo Hurle, Paolo Casale, Enrico Lugli, Ronald A. DePinho, Subhankar Mukhopadhyay, Siamon Gordon, Diletta Di Mitri
Summary: A subset of tumor-associated macrophages in prostate cancer is characterized by dysregulation in transcriptional pathways associated with lipid metabolism and correlates with cancer progression and shorter disease-free survival. Cancer cell-derived IL-1 beta enhances macrophage Marco expression, while lipid-loaded TAMs promote cancer cell migration through the release of CCL6. Targeting lipid accumulation by Marco blockade hinders tumor growth and invasiveness, improving chemotherapy efficacy in prostate cancer models.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Immunology
Valeria Fumagalli, Micol Rava, Davide Marotta, Pietro Di Lucia, Chiara Laura, Eleonora Sala, Marta Grillo, Elisa Bono, Leonardo Giustini, Chiara Perucchini, Marta Mainetti, Alessandro Sessa, Jose M. Garcia-Manteiga, Lorena Donnici, Lara Manganaro, Serena Delbue, Vania Broccoli, Raffaele De Francesco, Patrizia D'Adamo, Mirela Kuka, Luca G. Guidotti, Matteo Iannacone
Summary: In this study, researchers described a method using an inhalation tower system to expose unanesthetized transgenic mice to aerosolized virus. This approach has advantages in simulating COVID-19 pathogenesis and provides a useful tool for studying viral transmission, disease pathogenesis, and therapeutic interventions.
SCIENCE IMMUNOLOGY
(2022)
Editorial Material
Behavioral Sciences
Patricia Pelufo Silveira, Lorenzo More, Carmem Gottfried
FRONTIERS IN BEHAVIORAL NEUROSCIENCE
(2022)
Article
Neurosciences
Lorenzo More, Lucia Privitera, Philippa Perrett, Daniel D. Cooper, Manuel Van Gijsel Bonnello, J. Simon C. Arthur, Bruno G. Frenguelli
Summary: The transcription factor CREB plays a crucial role in regulating physiological functions in the central nervous system. Recent research has focused on the phosphorylation of the S133 residue in CREB, which is required for its transcriptional activation. Previous studies using molecular genetic techniques have resulted in conflicting results, possibly due to the manipulation of endogenous CREB. In this study, the researchers generated a postnatal and forebrain-specific mutant of CREB S133A to avoid potential complications. The findings show that CREB S133 is necessary for spatial cognitive flexibility, basal synaptic transmission, and long-term potentiation in the hippocampus, highlighting its importance in neuronal function, synaptic plasticity, and cognition.
Article
Behavioral Sciences
Ilaria M. Morella, Riccardo Brambilla, Lorenzo More
Summary: This article discusses the impact of diverse environmental manipulations on cognition, with a focus on psychiatric conditions. The authors present evidence supporting a direct causal correlation between environmental stimulation and psychopathological traits, as well as how the environment affects brain structure and function, particularly in relation to oxidative stress and mitochondrial activity.
NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS
(2022)
Meeting Abstract
Oncology
Michela Masetti, Federica Portale, Roberta Carriero, Bianca Partini, Nicolo Morina, Andrea Ponzetta, Piergiuseppe Colombo, Maria Grazia Elefante, Alberto Saita, Giovanni Lughezzani, Nicolo' Buffi, Paolo Casale, Clelia Peano, Paolo Kunderfranco, Javier Cibella, Giorgio Ferruccio Guazzoni, Massimo Lazzeri, Diletta Di Mitri
JOURNAL OF CLINICAL ONCOLOGY
(2020)
Meeting Abstract
Urology & Nephrology
Massimo Lazzeri, Pieve Emanuele, Michela Masetti, Federica Portale, Roberta Carriero, Bianca Partini, Andrea Ponzetta, Sebastien Jaillon, Piergiuseppe Colombo, Grazia Elefante, Alberto Saita, Paolo Casale, Clelia Peano, Paolo Kunderfranco, Javier Cibella, Giorgio Guazzoni, Diletta Di Mitri
JOURNAL OF UROLOGY
(2020)
Article
Behavioral Sciences
Edna C. Cieslik, Markus Ullsperger, Martin Gell, Simon B. Eickhoff, Robert Langner
Summary: Previous studies on error processing have primarily focused on the posterior medial frontal cortex, but the role of other brain regions has been underestimated. This study used activation likelihood estimation meta-analyses to explore brain activity related to committing errors and responding successfully in interference tasks. It was found that the salience network and the temporoparietal junction were commonly involved in both correct and incorrect responses, indicating their general involvement in coping with situations that require increased cognitive control. Error-specific convergence was observed in the dorsal posterior cingulate cortex, posterior thalamus, and left superior frontal gyrus, while successful responding showed stronger convergence in the dorsal attention network and lateral prefrontal regions. Underrecruitment of these regions in error trials may reflect failures in activating the appropriate stimulus-response contingencies necessary for successful response execution.
NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS
(2024)
