Article
Biology
Paul Shin, Qi Pian, Hidehiro Ishikawa, Gen Hamanaka, Emiri T. Mandeville, Shuzhen Guo, Buyin Fu, Mohammed Alfadhel, Srinivasa Rao Allu, Ikbal Sencan-Egilmez, Baoqiang Li, Chongzhao Ran, Sergei A. Vinogradov, Cenk Ayata, Eng Lo, Ken Arai, Anna Devor, Sava Sakadzic, Daniel Henrion
Summary: Aging is a risk factor for cognitive impairment. Aerobic exercise benefits brain function and may promote cognitive health in older adults. This study found that aerobic exercise can improve cerebral microcirculation and cognitive function in aging individuals.
Review
Chemistry, Analytical
Vinayak Juvekar, Hyo Won Lee, Dong Joon Lee, Hwan Myung Kim
Summary: Two-photon microscopy is an important tool for deep-tissue imaging, providing high-resolution fluorescence images beyond millimeter depths. Recent research has focused on developing innovative strategies and probes for quantitative analysis using two-photon fluorescence.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Engineering, Biomedical
Yongchao Wang, Guangru Liang, Fei Liu, Qian Chen, Lei Xi
Summary: In this study, a transparent, acoustically and optically transparent, long-term and biocompatible cranial window was designed, fabricated, and evaluated for high-resolution photoacoustic microscopy in rat cerebral cortex. The cranial window made with PDMS demonstrated better imaging quality compared to conventional cover-glass-based cranial window, with an optimal film thickness of 50 μm. The window maintained good quality and stability for long-term in vivo monitoring of rat cerebral cortex.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2021)
Article
Neurosciences
Marine Tournissac, Davide Boido, Manon Omnes, Yannick Goulam Houssen, Luisa Ciobanu, Serge Charpak
Summary: This article introduces a transparent, stable, and inexpensive chronic cranial window preparation that is suitable for multimodal imaging, allowing the study of different imaging modalities in the same animal.
Article
Immunology
Han-Dong Li, Ran Li, Ying Kong, Wenyan Zhang, Caiyun Qi, Dan Wang, Hongying Hao, Qiang Liu
Summary: Results from this study demonstrate that the use of a selective S1PR1 modulator, Ozanimod, significantly enhances the thrombolytic effect of a sub-thrombolytic dose of tPA, improving the therapeutic benefit for patients with acute ischemic stroke.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Chemistry, Inorganic & Nuclear
Vinayak Juvekar, Sang Jun Park, Juyoung Yoon, Hwan Myung Kim
Summary: Two-photon microscopy combined with two-photon fluorescent probes enables visualization of metal ion distribution in live tissues, facilitating the development of probes for metal ion detection. Recent progress between 2014 and 2020, including design strategies, history, and specific applications, was summarized in this review.
COORDINATION CHEMISTRY REVIEWS
(2021)
Editorial Material
Biochemical Research Methods
Jerome A. Lecoq, Roman Boehringer, Benjamin F. Grewe
Summary: New three-photon miniature microscopes enable the study of neuronal networks in the deep regions of the brains of behaving animals.
Article
Neurosciences
Vanessa Coelho-Santos, Taryn Tieu, Andy Y. Shih
Summary: This study describes a non-invasive cranial window procedure for longitudinal imaging of neonatal mice. By thinning the skull and reinforcing the window, in vivo imaging of the brain can be achieved without disrupting the intracranial environment. This technique is significant for studying both development and pathological states.
Article
Multidisciplinary Sciences
Giovanni Barbera, Rachel Jun, Yan Zhang, Bo Liang, Yun Li, Da-Ting Lin
Summary: In this work, a lightweight one-photon microscope capable of imaging at different focal depths is presented. The focal plane can be dynamically changed by modulating the pulse width of the control signal, enabling the switching of focal plane between frames. Experimental results showed an increase in the number of imaged neurons compared to single plane imaging.
SCIENTIFIC REPORTS
(2022)
Review
Immunology
Tuula Penate Medina, Jan Philip Kolb, Gereon Huttmann, Robert Huber, Oula Penate Medina, Linh Ha, Patricia Ulloa, Naomi Larsen, Arianna Ferrari, Magdalena Rafecas, Mark Ellrichmann, Mariya S. Pravdivtseva, Mariia Anikeeva, Jana Humbert, Marcus Both, Jennifer E. Hundt, Jan-Bernd Hovener
Summary: This article reviews the recent advances in imaging technologies and their potential applications in the medical field, focusing on the development of imaging in precision medicine for inflammation. It also analyzes the challenges and opportunities faced by imaging technologies, showcasing some current or near-clinical applications as examples.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Physics, Applied
Hui Li, Yixuan Yin, Feng Xiang, Jia Yu, Ting Wu, Lina Liu, Jiuling Liao, Wei Zheng
Summary: Detection of cerebral ischemia using two-photon FLIM with NADH as an indicator shows sensitivity to metabolic changes and consistency with ischemic time. This approach provides a promising method for early characterization of degrees of cerebral ischemia and related injuries.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2021)
Article
Biochemical Research Methods
Bowen Wei, Chenmao Wang, Zongyue Cheng, Baoling Lai, Wen-Biao Gan, Meng Cui
Summary: COMPACT allows imaging of large volumes in the brain through a periscope-like probe inside a glass capillary, enabling observation of the dynamics of neuronal structure and function in different regions. This technology provides a valuable tool for understanding the neural circuit mechanisms underlying behavior.
Article
Endocrinology & Metabolism
Nao Hatakeyama, Miyuki Unekawa, Juri Murata, Yutaka Tomita, Norihiro Suzuki, Jin Nakahara, Hiroyuki Takuwa, Iwao Kanno, Ko Matsui, Kenji F. Tanaka, Kazuto Masamoto
Summary: The study investigated the effects of different types of brain cells on cerebrovascular responses using optogenetics in the cortex of mice. It found that astrocytes and neurons induced different patterns of cerebral blood flow increase when activated, with the former causing a widespread increase and the latter causing a localized increase.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2021)
Article
Multidisciplinary Sciences
Wanjie Wu, Sicong He, Junqiang Wu, Congping Chen, Xuesong Li, Kai Liu, Jianan Y. Qu
Summary: The spinal cord serves as the main communication pathway between the brain and peripheral nervous system, but spinal cord injury is a devastating and irreversible trauma. Researchers have developed a minimally invasive intervertebral window and an optical clearing method to enable long-term, stable, high-resolution imaging of the mouse spinal cord without inducing inflammation. This method provides a reliable platform for studying spinal cord pathology and identifying effective treatments.
NATURE COMMUNICATIONS
(2022)
Review
Chemistry, Multidisciplinary
Dong Joon Lee, Eun Seo Kim, Hyo Won Lee, Hwan Myung Kim
Summary: This mini-review summarizes the recent advances in two-photon fluorescent trackers for monitoring lipid droplets (LDs). The article covers the chemical structures, two-photon properties, live sample imaging, and biomedical applications of representative two-photon fluorescent trackers for LDs. The current challenges and future research trends for two-photon fluorescent trackers of LDs are also discussed.
FRONTIERS IN CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Ken-ichiro Matsumoto, Ikuo Nakanishi, Zhivko Zhelev, Rumiana Bakalova, Ichio Aoki
Summary: The use of nitroxyl radicals based on a cyclic redox mechanism as an index of tissue redox status has significant implications. Recent advances have focused on developing in vivo redox imaging using nitroxyl radicals as redox-sensitive contrast agents. Additionally, the development of nitroxyl radical contrast agents and drugs for theranostics has shown promise.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Endocrinology & Metabolism
Yuhei Takado, Hiroyuki Takuwa, Kazuaki Sampei, Takuya Urushihata, Manami Takahashi, Masafumi Shimojo, Shoko Uchida, Nobuhiro Nitta, Sayaka Shibata, Keisuke Nagashima, Yoshihiro Ochi, Maiko Ono, Jun Maeda, Yutaka Tomita, Naruhiko Sahara, Jamie Near, Ichio Aoki, Kazuhisa Shibata, Makoto Higuchi
Summary: The study aims to investigate whether MRS-measured Glutamate (Glu) and GABA can reflect excitatory and inhibitory neural activities of neurons in an awake condition. Results showed that while excitatory neuronal activities increased during prolonged sensory stimulations, inhibitory neuronal activities remained at baseline levels in the first session, and in the second half, inhibitory activities were significantly enhanced. This suggests that MRS signals can reflect regional neurochemical statuses that align with neural activities, providing insight into the neurochemistry of neuron populations in awake mice.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2022)
Article
Oncology
Donika Ivanova, Zhivko Zhelev, Genoveva Zlateva, Dessislava Lazarova, Zvezdelina Yaneva, Radmila Panovska, Ichio Aoki, Rumiana Bakalova
Summary: This study analyzed the effects of alpha-tocopheryl succinate (alpha-TS) on the redox-state of leukemia and normal lymphocytes, as well as their sensitivity to anticancer drugs. It found that combination of alpha-TS with certain drugs showed a synergistic cytotoxic effect on leukemia lymphocytes, but had no effect on normal lymphocytes. Therefore, alpha-TS could be a promising adjuvant therapy in second-line anticancer treatment.
ANTICANCER RESEARCH
(2022)
Review
Cell Biology
Zhivko Zhelev, Ichio Aoki, Dessislava Lazarova, Tatyana Vlaykova, Tatsuya Higashi, Rumiana Bakalova
Summary: Cancer metabolism is a fascinating and complex field that involves abnormal activation of various metabolic pathways. Recent studies have found pronounced mitochondrial fatty acid oxidation in many types of cancer cells. This article discusses how this process can escape normal regulation and be overactivated under certain conditions, and proposes a new metabolic pathway called the beta-oxidation shuttle.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Chemistry, Multidisciplinary
Rohmad Yudi Utomo, Satoshi Okada, Akira Sumiyoshi, Ichio Aoki, Hiroyuki Nakamura
Summary: In this study, a curcumin derivative conjugated with Gd-DO3A was synthesized as an MRI contrast agent for detecting the fibrillation process of amyloid-beta. Gd-DO3A-Comp.B significantly inhibited the aggregation of Aβ and detected fibril growth using T1-weighted MR imaging at lower concentrations.
Article
Cell Biology
Maiko Ono, Masaaki Komatsu, Bin Ji, Yuhei Takado, Masafumi Shimojo, Takeharu Minamihisamatsu, Eiji Warabi, Toru Yanagawa, Gen Matsumoto, Ichio Aoki, Nicholas M. Kanaan, Tetsuya Suhara, Naruhiko Sahara, Makoto Higuchi
Summary: In this study, evidence is provided that p62 exerts a protective role against neuronal death and neuroinflammation induced by abnormal tau accumulation. By eliminating neurotoxic tau species, p62 may act as a potential therapeutic target for the treatment of tauopathies.
Article
Biochemistry & Molecular Biology
Rumiana Bakalova, Ichio Aoki, Zhivko Zhelev, Tatsuya Higashi
Summary: Recent studies have shown that impaired respiration can lead to redox imbalance in NAD(+)/NADH and NADP(+)/NADPH pairs, triggering two metabolic pathways that affect mitochondrial function, senescence, and cell proliferation. The beta-oxidation shuttle disrupts the balance of NAD+/NADH in mitochondria, while the hydride transfer complex (HTC) cycle maintains the balance of NAD(+)/NADH in the cytoplasm and disrupts the balance of NADP(+)/NADPH. Senescence is influenced by high cytoplasmic NADH but low NADPH, while proliferation depends on high levels of cytoplasmic NAD(+) and NADPH, which are regulated by mitochondria. Therefore, controlling the HTC cycle is crucial for determining cell fate - senescence or proliferation.
Article
Neurosciences
Mitsuhiro Oishi, Stefan Passlick, Yoshihiko Yamazaki, Miyuki Unekawa, Ruka Adachi, Mayumi Yamada, Itaru Imayoshi, Yoshifumi Abe, Christian Steinhaeuser, Kenji F. Tanaka
Summary: Nerve/glial antigen 2 (NG2) is a protein marker that can be used to target specific cell types in transgenic mice. By combining different gene induction systems, researchers have developed a method to selectively control the function of mural cells and NG2 glia. This research provides valuable tools and methods for studying the function and mechanisms of these cells.
Correction
Biochemistry & Molecular Biology
Rumiana Bakalova, Ichio Aoki, Zhivko Zhelev, Tatsuya Higashi
Article
Radiology, Nuclear Medicine & Medical Imaging
Nobuhiko Iki, Ryuta Nakane, Atsuko Masuya-Suzuki, Yoshikazu Ozawa, Takako Maruoka, Megumi Iiyama, Akira Sumiyoshi, Ichio Aoki
Summary: In this study, a manganese-based metal organic framework (MOF) with a safe paramagnetic center and high relaxivity was proposed. The MOF, modified with poly(ethylene glycol), could be stably dispersed in water and passively delivered to tumor tissues. In vivo imaging showed significant signal enhancement in T1-weighted images for tumor recognition.
MOLECULAR IMAGING AND BIOLOGY
(2023)
Article
Oncology
Donika Ivanova, Severina Semkova, Zvezdelina Yaneva, Biliana Nikolova, Zhivko Zhelev, Rumiana Bakalova, Ichio Aoki
Summary: This study aimed to investigate the ability of DHA to enhance the anticancer effect of M/A by increasing ROS production and affecting steady-state ATP levels. The results showed that the triple combination of M/A/DHA exhibited higher antiproliferative activity and cytotoxicity compared to M/A and DHA alone. DHA significantly accelerated M/A-induced ATP depletion and increased mitochondrial superoxide. These findings suggest that DHA enhances M/A-induced cytotoxicity by inducing mitochondrial oxidative stress and ATP depletion.
ANTICANCER RESEARCH
(2023)
Article
Clinical Neurology
Juri Murata, Miyuki Unekawa, Yuya Kudo, Maho Kotani, Iwao Kanno, Yoshikane Izawa, Yutaka Tomita, Kenji F. Tanaka, Jin Nakahara, Kazuto Masamoto
Summary: This study found that cerebral microvascular obstruction is related to recurrent stroke and decreased cerebral blood flow with age. The study showed a relationship between capillary lumen space and embolism formation. Constriction of capillaries increases the risk of developing microcirculation embolism.
Article
Biochemistry & Molecular Biology
Mamoru Shibata, Satoshi Kitagawa, Miyuki Unekawa, Tsubasa Takizawa, Jin Nakahara
Summary: Migraine is a debilitating neurovascular disorder characterized by recurrent headache attacks. Calcitonin gene-related peptide (CGRP) plays a crucial role in migraine pathogenesis. In a CSD-based mouse migraine model, CSD did not significantly change the density of CGRP mRNA-synthesizing neurons in the trigeminal ganglion (TG), but it did cause an increase in cell sizes of CGRP mRNA-synthesizing TG neurons. The findings suggest that the increase in cell size may contribute to increased disease activity and reduced responsiveness to CGRP-based therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Haruto Sugawara, Toshihiro Furuta, Akira Sumiyoshi, Megumi Iiyama, Masaru Kamitani, Aiko Suzuki, Arao Murakami, Osamu Abe, Ichio Aoki, Hiroyuki Akai
Summary: In this study, a CT lymphangiography method was established in mice via direct lymph node puncture. The results showed that the contrast ratios and visual assessment scores of lymph nodes and lymphatic vessels were significantly better in the direct puncture group compared to the CT or MR interstitial injection method.
EUROPEAN RADIOLOGY
(2023)
Article
Nutrition & Dietetics
Megumi Ueno, Sayaka Shibata, Ikuo Nakanishi, Ichio Aoki, Ken-Ichi Yamada, Ken-Ichiro Matsumoto
Summary: This study investigated the impact of radiation-induced hydrogen peroxide on the biological effects of X-rays and carbon-ion beams using a selenium-deficient mouse model. The results suggest that selenium deficiency slightly alters the biological effects of these two types of radiation.
JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION
(2023)
Article
Neurosciences
Yang He, Jun Tang, Meng Zhang, Junjie Ying, Dezhi Mu
Summary: This study investigated the protective effects and mechanisms of human placenta derived mesenchymal stem cells (hPMSCs) transplantation in a rat model of hypoxic-ischemic encephalopathy (HIE). The results showed that hPMSCs transplantation reduced apoptosis and improved long-term neurological prognosis. Furthermore, the downregulation of Sema 3A/NRP-1 expression and activation of the PI3K/Akt/mTOR signaling pathway played a key role in the protective effects of hPMSCs.
Article
Neurosciences
Emily L. Isenstein, Edward G. Freedman, Jiayi Xu, Ian A. DeAndrea-Lazarus, John J. Foxe
Summary: This study evaluated electrophysiological discrimination of parametric somatosensory stimuli in healthy young adults to understand how the brain processes the duration of tactile information. The results showed that participants did not electrophysiologically discriminate between 100 and 115 ms, but they exhibited distinct electrophysiological responses when the deviant stimuli were 130, 145, and 160 ms. These findings contribute to a better understanding of tactile sensitivity in different clinical conditions.
Article
Neurosciences
Juliana R. Souza, Ludmila Lima-Silveira, Daniela Accorsi-Mendonca, Benedito H. Machado
Summary: This study demonstrates that A2A receptors play a crucial role in modulating synaptic transmission in the NTS neurons and are required for the enhancement of glutamatergic transmission observed under short-term sustained hypoxia conditions.
Article
Neurosciences
Miki Hashizume, Rina Ito, Rie Suge, Yasushi Hojo, Gen Murakami, Takayuki Murakoshi
Summary: The basolateral amygdaloid complex (BLA) is closely involved in the formation of emotional memories, including both aversive memory and contextual fear memory. Acute sleep deprivation (SD) disrupts the acquisition of tone-associated fear memory in juvenile rats, but has no significant effect on contextual fear memory. Slow network oscillation in the amygdala contributes to the formation of amygdala-dependent fear memory in relation to sleep.
Article
Neurosciences
Qunxian Wang, Shipeng Guo, Dongjie Hu, Xiangjun Dong, Zijun Meng, Yanshuang Jiang, Zijuan Feng, Weihui Zhou, Weihong Song
Summary: GSDME plays a crucial role in the pathogenesis of Alzheimer's disease by regulating the switch from apoptosis to pyroptosis and participating in neuroinflammatory response. Knockdown of GSDME has been shown to improve cognitive impairments, indicating that GSDME could be a therapeutic target for Alzheimer's disease.