期刊
NEUROSCIENCE
卷 175, 期 -, 页码 300-314出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2010.11.064
关键词
alpha-adrenergic receptor; sensory afferent; skin; dorsal root ganglion; immunohistochemistry
资金
- National Health and Medical Research Council of Australia
- Australian and New Zealand College of Anaesthetists
- Medtronic Australasia Pty Ltd.
The purpose of this study was to determine whether alpha(1)-adrenoceptors are expressed on primary nociceptive afferents that innervate healthy skin. Skin and dorsal root ganglia were collected from adult male Wistar rats and assessed using fluorescence immunohistochemistry with antibodies directed against alpha(1)-adrenoceptors alone or in combination with specific labels including myelin basic protein and neurofilament 200 (markers of myelinated nerve fibres), protein gene product 9.5 (a pan-neuronal marker), tyrosine hydroxylase (sympathetic neurons), isolectin B-4 (IB4: non-peptidergic sensory neurons), calcitonin gene related peptide (CGRP) and transient receptor potential vanilloid receptor 1 (TRPV1) (peptidergic sensory neurons). Double labelling in dorsal root ganglia confirmed the expression of alpha(1)-adrenoceptors within sub-populations of CGRP, IB4 and TRPV1 immunoreactive neurons. Myelinated and unmyelinated sensory nerve fibres in the skin expressed alpha(1)-adrenoceptors whereas sympathetic nerve fibres did not. The expression of alpha(1)-adrenoceptors on C- and A-delta nociceptive afferent fibres provides a histochemical substrate for direct excitation of these fibres by adrenergic agonists. This may help to explain the mechanism of sensory-sympathetic coupling that sometimes develops on surviving primary nociceptive afferents in neuropathic pain states. (c) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.
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