Article
Biochemistry & Molecular Biology
Agata Piasecka, Andrea Baier
Summary: This study investigated the impact of three trophic modes on the growth parameters and biochemical composition of Chlorella species. The results showed that mixotrophic cultivation using an agro-industrial by-product stimulated the growth of all species, yielding high amounts of protein-rich biomass. The study also provided insights into the proteome of Chlorella cells cultured under molasses supplementation conditions.
Article
Biochemical Research Methods
Shakila H. Peli Thanthri, Thomas H. Linz
Summary: This report presents a microfluidic thermal gel transient isotachophoresis (TG-tITP) method for rapid and high-resolution separation of native proteins. By utilizing the viscosity response of thermal gels and temperature gradients, this method achieves a wide mass range separation with 15,000-fold less protein loading and five-fold faster analysis times.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Rijul Bhimwal, Richard R. Rustandi, Anne Payne, Mohamed Dawod
Summary: This review highlights the noteworthy advancements in capillary gel electrophoresis for protein separation and analysis from 2015 to 2021. It provides an overview of the technique's historical perspective, principles, challenges, and limitations, and discusses the advancements made to overcome these issues and expand our understanding of the method. The review also presents applications of capillary gel electrophoresis and future directions for the technique.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Plant Sciences
Zheng-Jun Guan, Min Zheng, Zhi-Xi Tang, Wei Wei, C. Neal Stewart Jr
Summary: In this study, differential expression of total protein in leaves of two transgenic oilseed rape lines and their non-transgenic parent plant was analyzed. Fourteen differential protein spots were identified, including eleven upregulated and three downregulated proteins, involved in photosynthesis, transporter function, metabolism, protein synthesis, and cell growth and differentiation. These changes in protein spots may be attributed to the insertion of foreign transgenes in transgenic oilseed rape, but transgenic manipulation does not necessarily cause significant changes in the proteomes of oilseed rape.
Article
Psychology, Mathematical
Yanfang Xia, Filip Melinscak, Dominik R. Bach
Summary: Threat-conditioned cues are believed to attract overt attention in a bottom-up process, with shorter scanpath length observed during presentation of threatening stimuli compared to non-threatening stimuli. This study suggests scanpath length as a visual search summary statistic which may be used to quantify threat conditioning with retrodictive validity similar to that of skin conductance responses.
BEHAVIOR RESEARCH METHODS
(2021)
Article
Neurosciences
May-Li Silva-Prieto, Julian I. Hofmann, Cornelius Schwarz
Summary: In mammals, multiple memory systems are involved in the learning and storage of associative memory. Through studying eyeblink conditioning in mice, it was found that specific neuronal activity in the primary somatosensory cortex exhibited learning-related suppression, which was independent of eyelid movements.
Article
Behavioral Sciences
Wiktor Bilecki, Joachim Latusz, Kinga Gawlinska, Magdalena Chmelova, Marzena Mackowiak
Summary: The study showed that short-term adolescent social isolation did not significantly affect fear memory and anxiety in adult rats, but altered protein levels related to synapse maturation and energy transfer. Additionally, the impact of adolescent social isolation on normally developing brains was different than those with a history of MAM administration.
BEHAVIOURAL BRAIN RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Jie Liang, William G. G. Tong
Summary: The carbohydrate antigen 19-9 (CA 19-9) serves as a commonly used biomarker for diagnosing pancreatic cancer. Multiphoton nonlinear laser wave-mixing spectroscopy is proposed as an extremely sensitive detection method for CA 19-9. This method allows for the detection of CA 19-9 without labels or with labeled samples, offering advantages such as short optical path length and high spatial resolution. The wave-mixing signal exhibits a quadratic dependence on sample concentration, making it an ideal sensor for monitoring small changes in the sample. When combined with capillary electrophoresis, laser wave-mixing spectroscopy provides enhanced selectivity levels for protein detection, surpassing the detection limits of chemiluminescence- or ELISA-based methods.
Article
Biochemistry & Molecular Biology
Zhiguo Li, Hong Shen, Yeqiang Liu, Xuefeng Zhou, Meihua Yan, Hailan He, Tingting Zhao, Haojun Zhang, Ping Li
Summary: This study explores the pathogenesis of DN by analyzing protein expression profile in OLETF rats, identifying mutations in genes encoding novel proteins related to oxidative stress and inflammation. These findings provide new potential targets for DN pathogenesis and enhance understanding of the disease.
Article
Environmental Sciences
Juan Wang, Lin Lin, Da-Zhi Wang
Summary: Quantitative proteomic analysis revealed that OA exposure caused liver damage, including dilation of rough endoplasmic reticulum and mitochondrial swelling in hepatocytes. Increased serum malondialdehyde content and decreased hepatic protein phosphatase activity were dose-dependently associated with OA exposure. Apoptotic cells in the liver showed a significant dose-response relationship.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Physical
Stefania Bergamini, Elisa Bellei, Luigi Generali, Aldo Tomasi, Carlo Bertoldi
Summary: This study evaluated proteomic changes induced by CHX and CHX+ADS in supragingival dental sites susceptible to discoloration effects. The results showed that long-term use of CHX led to changes in the TSCM proteome, but these changes were reduced when using CHX+ADS. The ADS limited TSCM formation and the antiseptic effect of CHX reduced its impact on bacterial cell permeability, while Maillard's reaction produced molecules that could facilitate bacterial adhesion.
Article
Biochemistry & Molecular Biology
Miao Chen, Frank Koopmans, Iryna Paliukhovich, Sophie J. F. van der Spek, Jian Dong, August B. Smit, Ka Wan Li
Summary: This study used an interaction proteomics workflow to reveal the existence of GABA(A) receptor subtypes containing both alpha 1 and alpha 6 subunits. Mass spectrometry analysis showed that these receptor subtypes exist in two main forms, with or without Neuroligin-2. Immunocytochemistry confirmed the presence of this synaptic GABA(A)R subtype at the synapse.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medical Laboratory Technology
Takafumi Kawanami, Haruka Kawanami-Iwao, Takanobu Takata, Yasuhito Ishigaki, Naohisa Tomosugi, Tsutomu Takegami, Hiroto Yanagisawa, Shino Fujimoto, Tomoyuki Sakai, Yoshimasa Fujita, Kazunori Yamada, Shuichi Mizuta, Hiroshi Kawabata, Toshihiro Fukushima, Yuko Hirose, Yasufumi Masaki
Summary: This study analyzed the changes in serum protein levels in patients with IgG4-related disease and found that LRG-1 could serve as a novel biomarker for this disease.
CLINICA CHIMICA ACTA
(2021)
Article
Microbiology
Deying Zou, Jiang Chang, Shiying Lu, Jianfeng Xu, Pan Hu, Kai Zhang, Xiaoli Sun, Wei Guo, Yansong Li, Zengshan Liu, Honglin Ren
Summary: This study compared the proteomes of A. baumannii strains and identified potential virulence factors associated with enhanced pathogenicity through protein-protein interaction network analysis, phylogenetic analysis, and animal infection models. The results showed that the pathogenicity of strain CCGGD201101 was increased by 180.5-fold compared to the standard strain. The study helps to better understand the mechanisms of virulence enhancement.
MICROBIOLOGICAL RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Ruben Agregan, Noemi Echegaray, Maria Lopez-Pedrouso, Radwan Kharabsheh, Daniel Franco, Jose M. Lorenzo
Summary: Proteomics has made significant advances in the field of medicine and has applications in other scientific areas, such as the study of milk. Milk proteomics can help characterize milk samples and products in detail, with significant applications in industry for quality control and improvement.
Article
Neurosciences
Yang He, Jun Tang, Meng Zhang, Junjie Ying, Dezhi Mu
Summary: This study investigated the protective effects and mechanisms of human placenta derived mesenchymal stem cells (hPMSCs) transplantation in a rat model of hypoxic-ischemic encephalopathy (HIE). The results showed that hPMSCs transplantation reduced apoptosis and improved long-term neurological prognosis. Furthermore, the downregulation of Sema 3A/NRP-1 expression and activation of the PI3K/Akt/mTOR signaling pathway played a key role in the protective effects of hPMSCs.
Article
Neurosciences
Emily L. Isenstein, Edward G. Freedman, Jiayi Xu, Ian A. DeAndrea-Lazarus, John J. Foxe
Summary: This study evaluated electrophysiological discrimination of parametric somatosensory stimuli in healthy young adults to understand how the brain processes the duration of tactile information. The results showed that participants did not electrophysiologically discriminate between 100 and 115 ms, but they exhibited distinct electrophysiological responses when the deviant stimuli were 130, 145, and 160 ms. These findings contribute to a better understanding of tactile sensitivity in different clinical conditions.
Article
Neurosciences
Juliana R. Souza, Ludmila Lima-Silveira, Daniela Accorsi-Mendonca, Benedito H. Machado
Summary: This study demonstrates that A2A receptors play a crucial role in modulating synaptic transmission in the NTS neurons and are required for the enhancement of glutamatergic transmission observed under short-term sustained hypoxia conditions.
Article
Neurosciences
Miki Hashizume, Rina Ito, Rie Suge, Yasushi Hojo, Gen Murakami, Takayuki Murakoshi
Summary: The basolateral amygdaloid complex (BLA) is closely involved in the formation of emotional memories, including both aversive memory and contextual fear memory. Acute sleep deprivation (SD) disrupts the acquisition of tone-associated fear memory in juvenile rats, but has no significant effect on contextual fear memory. Slow network oscillation in the amygdala contributes to the formation of amygdala-dependent fear memory in relation to sleep.
Article
Neurosciences
Qunxian Wang, Shipeng Guo, Dongjie Hu, Xiangjun Dong, Zijun Meng, Yanshuang Jiang, Zijuan Feng, Weihui Zhou, Weihong Song
Summary: GSDME plays a crucial role in the pathogenesis of Alzheimer's disease by regulating the switch from apoptosis to pyroptosis and participating in neuroinflammatory response. Knockdown of GSDME has been shown to improve cognitive impairments, indicating that GSDME could be a therapeutic target for Alzheimer's disease.