期刊
NEUROSCIENCE
卷 164, 期 4, 页码 1496-1508出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2009.09.080
关键词
zebrin II; Ebf2; scrambler; pattern formation; cerebellar development
资金
- Canadian Institutes of Health Research (RH)
- Fondazione Mariani, Milan, Italy
Cerebellar unipolar brush cells (UBCs) are glutamatergic interneurons of the granular layer. Previous studies have identified three distinct LIBC subsets in the mouse cerebellar cortex: one expressing the calcium-binding protein calretinin (CR), a second expressing both the metabotropic glutamate receptor (mGluR)1 alpha and phospholipase C(PLC)beta 4, and a third expressing PLC beta 4 but not mGluR1 alpha. We have investigated UBC topography in two strains of mutant mice: early B-cell factor 2 (Ebf2) null and scrambler. In Ebf2 null mice Purkinje cell topography is disrupted due to Purkinje cell death and ectopic gene expression. The topography of all three classes of UBCs is also abnormal: the CR+ UBCs, which are normally aligned with zebrin II stripes, become homogeneously distributed; the numerical density of mGluRl alpha(+) UBCs is increased; and many PLC beta 4(+) UBCs are located ectopically. The LIBC ectopia is not a cell-intrinsic action of the Ebf2 gene-analysis of the constitutive expression of a beta-galactoside reporter under the control of the Ebf2 promoter reveals no Ebf2 expression in UBCs at any stage of cerebellar development. In scrambler (Dab1(scm)), most Purkinje cells are ectopic but nevertheless have normal adult gene expression patterns. In scrambler, UBCs associate with specific ectopic Purkinje cell clusters. Finally, similar associations with specific Purkinje cell clusters are seen during normal cerebellar development. These data suggest that UBCs become regionally restricted during development through a non-cell-autonomous mechanism involving embryonic interactions with different Purkinje cell subtypes. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.
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