☆ 4.3 Article

Scaffolding proteins in highly purified rat olfactory cilia membranes

NEUROREPORT (2008)

期刊

NEUROREPORT

卷 19, 期 11, 页码 1123-1126

出版社

LIPPINCOTT WILLIAMS & WILKINS

DOI: 10.1097/WNR.0b013e3283086797

关键词

olfactory epithelium; scaffolding proteins; signal transduction; subcellular fractionation

类别

Neurosciences

向作者/读者索取更多资源

Protocol

Reagent

摘要

Odour-mediated signal transduction is a complex process that occurs in the cilia of olfactory sensory neurons. To gain insight in to the molecular organization of the odour transduction machinery, we developed a procedure to purify olfactory cilia membranes by differential centrifugation of rat olfactory epithelium extracts. We tested whether known scaffolding proteins that might participate in the assembly of the complex chemotransduction apparatus are present in the purified membrane fraction. Utilizing immunoblotting and immunohistochemistry, we show that the multidomain scaffolding proteins ProSAP/Shanks and calcium/ calmodulin-dependent serine protein kinase CASK are present in the olfactory cilia. Ion channels involved in chemotransduction could be reconstituted into planar lipid bilayers for electrophysiological recordings. Our procedure should allow the identification of further chemotransduction-related proteins. NeuroReport 19:1123-1126 (C) 2008 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.

作者

我是这篇论文的作者

点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.3

评分不足

次要评分

新颖性

-

重要性

-

科学严谨性

-

评价这篇论文

推荐

Article Biochemistry & Molecular Biology

Keratin 8 is a scaffolding and regulatory protein of ERAD complexes

Iwona Maria Pranke, Benoit Chevalier, Aiswarya Premchandar, Nesrine Baatallah, Kamil F. Tomaszewski, Sara Bitam, Danielle Tondelier, Anita Golec, Jan Stolk, Gergely L. Lukacs, Pieter S. Hiemstra, Michal Dadlez, David A. Lomas, James A. Irving, Agnes Delaunay-Moisan, Eelco van Anken, Alexandre Hinzpeter, Isabelle Sermet-Gaudelus, Aleksander Edelman

Summary: Early recognition and enhanced degradation of misfolded proteins by the endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD) are important mechanisms. This study found that decreasing Keratin 8 (K8) expression increased the secretion of misfolded Z-alpha-1-antitrypsin (Z-A1AT) protein and F508del-CFTR protein. K8 was shown to regulate the Hrd1-governed ERAD pathway and may act as a scaffolding protein in ERAD complexes. Targeting K8-containing ERAD complexes could be a promising strategy for the treatment of alpha-1-antitrypsin deficiency (A1ATD).

CELLULAR AND MOLECULAR LIFE SCIENCES (2022)

添加到收藏夹

Article Cell Biology

Subtype-dependent regulation of Gβγ signalling

Mithila Tennakoon, Kanishka Senarath, Dinesh Kankanamge, Kasun Ratnayake, Dhanushan Wijayaratna, Koshala Olupothage, Sithurandi Ubeysinghe, Kimberly Martins-Cannavino, Terence E. Hebert, Ajith Karunarathne

Summary: G protein-coupled receptors transmit information to the cell interior through the diversity of Gα and Gβ subtypes, affecting downstream effector molecules. Studies have found preferences of distinct Gβγ combinations for specific GPCRs, Gα subtypes, and effector molecules.

CELLULAR SIGNALLING (2021)

添加到收藏夹

Review Microbiology

Illuminating the oral microbiome: cellular microbiology

Richard J. Lamont, Daniel P. Miller, Juhi Bagaitkar

Summary: Epithelial cells serve as both a barrier and an interactive interface with microbial colonization. The study of cellular microbiology has provided insights into epithelial cell physiology, bacterial colonization and pathogenic strategies, and innate host responses.

FEMS MICROBIOLOGY REVIEWS (2023)

添加到收藏夹

Article Multidisciplinary Sciences

Low signaling efficiency from receptor to effector in olfactory transduction: A quantified ligand-triggered GPCR pathway

Rong-Chang Li, Laurie L. Molday, Chih-Chun Lin, Xiaozhi Ren, Alexander Fleischmann, Robert S. Molday, King-Wai Yau

Summary: This study provides evidence that the ligand-triggered GPCR pathway in mammalian olfactory transduction produces less than one downstream effector when an odorant-receptor molecule is complexed with its most effective odorants. This finding suggests a potential paradigm shift in our understanding of GPCR signaling.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2022)

添加到收藏夹

Review Plant Sciences

The integration of reactive oxygen species (ROS) and calcium signalling in abiotic stress responses

Barkha Ravi, Christine H. Foyer, Girdhar K. Pandey

Summary: Reactive oxygen species (ROS) and calcium (Ca2+) signalling play important roles in plant growth, development, and defense. They function together with electric signals in directional cell-to-cell systemic signalling and even plant-to-plant communication. However, there is still limited understanding of the molecular mechanisms underlying the management of ROS and Ca2+ signals, as well as the synchronous and independent signaling in different cellular compartments. This review focuses on the proteins that may connect different pathways during abiotic stress responses, and highlights the crosstalk between ROS and Ca2+ pathways in cell signaling.

PLANT CELL AND ENVIRONMENT (2023)

添加到收藏夹

Article Medicine, Research & Experimental

SARS-CoV-2 infection of sustentacular cells disrupts olfactory signaling pathways

Abhishek Kumar Verma, Jian Zheng, David K. Meyerholz, Stanley Perlman

Summary: Loss of olfactory function in SARS-CoV-2 infections is associated with sustentacular cell infection and inflammation. Infection leads to damage and loss of cilia on olfactory sensory neurons (OSNs) and downregulation of olfactory receptors and signal transduction molecules. Recovery of olfactory function takes about 3-4 weeks, and not all animals fully recovered. Similar patterns of olfactory infection and dysfunction were observed in mice infected with other human coronaviruses.

JCI INSIGHT (2022)

添加到收藏夹

Article Biophysics

A biohybrid nose for evaluation of odor masking in the peripheral olfactory system

Liujing Zhuang, Xinwei Wei, Nan Jiang, Qunchen Yuan, Chunlian Qin, Deming Jiang, Mengxue Liu, Yanning Zhang, Ping Wang

Summary: The study introduces a biohybrid nose for evaluating malodor masking efficiency of perfumed products objectively and quantitatively. Results show that the masking efficiency of fragrance is concentration-dependent, with complete masking potentially occurring when fragrance is used at a concentration 2-3 orders of magnitude higher than malodor.

BIOSENSORS & BIOELECTRONICS (2021)

添加到收藏夹

Article Biochemistry & Molecular Biology

Mandipropamid as a chemical inducer of proximity for in vivo applications

Michael J. Ziegler, Klaus Yserentant, Valentin Dunsing, Volker Middel, Antoni J. Gralak, Kaisa Pakari, Joern Bargstedt, Christoph Kern, Annett Petrich, Salvatore Chiantia, Uwe Strahle, Dirk-Peter Herten, Richard Wombacher

Summary: The agrochemical mandipropamid (Mandi) has been shown to be a highly efficient chemical inducer of protein proximity (CIP) in cell culture systems and living organisms, with high specificity and orthogonality. It is capable of rapid and efficient protein translocation and network manipulation.

NATURE CHEMICAL BIOLOGY (2022)

添加到收藏夹

Article Biochemical Research Methods

CODEX multiplexed tissue imaging with DNA-conjugated antibodies

Sarah Black, Darci Phillips, John W. Hickey, Julia Kennedy-Darling, Vishal G. Venkataraaman, Nikolay Samusik, Yury Goltsev, Christian M. Schuerch, Garry P. Nolan

Summary: Advances in multiplexed imaging technologies, such as CODEX, using DNA-conjugated antibodies and fluorescently labeled DNA probes, allow visualization of up to 60 markers simultaneously in tissues. This enables a deep view into single-cell spatial relationships and can drive discovery in developmental biology, disease, and therapeutic design.

NATURE PROTOCOLS (2021)

添加到收藏夹

Article Biology

Mapping transgene insertion sites reveals the α-Cre transgene expression in both developing retina and olfactory neurons

Yimeng Fan, Wenyue Chen, Ran Wei, Wei Qiang, Joel D. Pearson, Tao Yu, Rod Bremner, Danian Chen

Summary: The study precisely mapped the insertion site of the Pax6-alpha-Cre transgene and found unexpected expression in olfactory neurons. The results indicate that the commonly used mouse line for retinal studies has transgene insertion within gene clusters of olfactory and vomeronasal receptors, leading to expression in not just retinal, but also olfactory and vomeronasal sensory neurons.

COMMUNICATIONS BIOLOGY (2022)

添加到收藏夹

Article Biochemical Research Methods

A Method to Estimate the Distribution of Proteins across Multiple Compartments Using Data from Quantitative Proteomics Subcellular Fractionation Experiments

Dirk F. Moore, David E. Sleat, Peter Lobel

Summary: Knowledge of cellular location is crucial for understanding protein function. Subcellular fractionation followed by mass spectrometry is a commonly used method for assigning cellular locations to proteins. However, proteins can reside in multiple compartments, which requires a more accurate method for localization. In this article, the constrained proportional assignment (CPA) method is introduced, along with data transformations and R-based programs for analyzing subcellular proteomics data.

JOURNAL OF PROTEOME RESEARCH (2022)

添加到收藏夹

Article Biodiversity Conservation

Relevance of cell subcompartmentalization techniques to predict adverse effects of metals in bivalves and fish

Alba Otero-Farina, Julie Retif, Isabelle Metais, Laurence Poirier, Amelie Chatel

Summary: Subcellular fractionation is a valuable technique for studying metal cell compartmentalization and evaluating the impact of contaminants in organisms. It provides important insights into the behavior of metals within cell compartments and their detoxification processes. This technique is particularly useful for studying the exposure and trophic transfer of metals in coastal and marine environments, using species like bivalves and fish as bioindicators. However, further research is needed to enhance the robustness of using subcellular fractionation for risk assessment.

ECOLOGICAL INDICATORS (2022)

添加到收藏夹

Article Multidisciplinary Sciences

Oligomeric scaffolding for curvature generation by ER tubule-forming proteins

Yun Xiang, Rui Lyu, Junjie Hu

Summary: Membrane curvature is generated by constructing a curved oligomeric scaffold formed by endoplasmic reticulum tubule-forming proteins. The mechanism of curvature generation remains elusive.

NATURE COMMUNICATIONS (2023)

添加到收藏夹

Review Cell Biology

The functional relevance of olfactory marker protein in the vertebrate olfactory system: a never-ending story

Michele Dibattista, Dolly Al Koborssy, Federica Genovese, Johannes Reisert

Summary: Olfactory marker protein (OMP) is expressed in olfactory receptor neurons in the nasal cavity and other chemosensory systems, playing a role in modulating odorant responses. Studies show that OMP is involved in regulating the kinetics of cAMP in olfactory transduction, but the specific mechanism is not fully understood yet.

CELL AND TISSUE RESEARCH (2021)

添加到收藏夹

Review Neurosciences

Sensory Transduction in Photoreceptors and Olfactory Sensory Neurons: Common Features and Distinct Characteristics

Federica Genovese, Johannes Reisert, Vladimir J. Kefalov

Summary: The past decades have seen significant progress in understanding the function of photoreceptors and olfactory sensory neurons. This progress has been driven by the combination of physiological experimental tools and genetic manipulations, which have allowed researchers to identify molecular players in sensory neuron transduction cascades, explore how properties impact stimulus detection and disease effects on vision and smell.

FRONTIERS IN CELLULAR NEUROSCIENCE (2021)

添加到收藏夹

Article Biochemistry & Molecular Biology

Arachidonic acid effect on the allosteric gating mechanism of BK (Slo1) channels associated with the β1 subunit

Pedro Martin, Melisa Moncada, Karen Castillo, Federico Orsi, Geronimo Ducca, Jose Manuel Fernandez-Fernandez, Carlos Gonzalez, Veronica Milesi

Summary: Arachidonic acid (AA) directly activates BK channels co-expressed with the auxiliary beta 1 subunit, stabilizing the voltage sensor domain (VSD) on its active configuration and facilitating channel opening transitions when VSD are at rest and CSD are unoccupied. The activation is independent of intracellular Ca2+ concentration and reduced when co-expressed with the Y74A mutant of the beta 1 subunit.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2021)

添加到收藏夹

Article Biotechnology & Applied Microbiology

An NADH preferring acetoacetyl-CoA reductase is engaged in poly-3-hydroxybutyrate accumulation in Escherichia coli

Karel Olavarria, Alexandre Carnet, Joachim van Renselaar, Caspar Quakkelaar, Ricardo Cabrera, Leonor Guedes da Silva, Aron L. Smids, Pablo Andres Villalobos, Mark C. M. van Loosdrecht, S. Aljoscha Wahl

Summary: The study demonstrates the possibility of producing PHB as a fermentation product in E. coli with higher efficiency. The importance of an acetoacetyl-CoA reductase with a preference for NADH in PHB production is highlighted through structural and kinetic analyses. The assembly of an operon combining phaCA genes with the gene encoding the NADH-preferring acetoacetyl-CoA reductase results in continuous accumulation of PHB in E. coli cells under oxygen limiting conditions, indicating opportunities for further protein/metabolic engineering strategies.

JOURNAL OF BIOTECHNOLOGY (2021)

添加到收藏夹

Article Cell Biology

eNOS-dependent S-nitrosylation of the NF-κB subunit p65 has neuroprotective effects

Ariel Caviedes, Barbara Maturana, Katherina Corvalan, Alexander Engler, Felipe Gordillo, Manuel Varas-Godoy, Karl-Heinz Smalla, Luis Federico Batiz, Carlos Lafourcade, Thilo Kaehne, Ursula Wyneken

Summary: This study reveals that NMDA receptor overstimulation leads to neuronal death, with NF-kappa B activity selectively enhanced in hippocampal neurons and inhibited through S-nitrosylation in resistant neurons. S-nitrosylation influences different biological processes in susceptible and resistant neurons.

CELL DEATH & DISEASE (2021)

添加到收藏夹

Article Multidisciplinary Sciences

Maternal exposure to a high-magnitude earthquake during pregnancy influences pre-reading skills in early childhood

Luis Federico Batiz, Yasna K. Palmeiro-Silva, Gregory E. Rice, Lara J. Monteiro, Albert M. Galaburda, Roberto Romero, Mahesh A. Choolani, Ursula Wyneken, Pelusa Orellana, Sebastian E. Illanes

Summary: This study found that maternal exposure to stressful events can lead to impaired pre-reading skills in children, especially those exposed to earthquakes during the first trimester of pregnancy. Females were more impacted in alphabet knowledge, whereas males were more affected in print knowledge skills.

SCIENTIFIC REPORTS (2021)

添加到收藏夹

Article Microbiology

Influenza A Virus (H1N1) Infection Induces Microglial Activation and Temporal Dysbalance in Glutamatergic Synaptic Transmission

Henning Peter Duesedau, Johannes Steffen, Caio Andreeta Figueiredo, Julia Desiree Boehme, Kristin Schultz, Christian Erck, Martin Korte, Heidi Faber-Zuschratter, Karl-Heinz Smalla, Daniela Dieterich, Andrea Kroeger, Dunja Bruder, Ildiko Rita Dunay

Summary: The study demonstrates that low-dose non-neurotrophic H1N1 strain of IAV infection causes a robust peripheral immune response followed by a temporary blood-brain barrier disturbance. Characterization of immune cells revealed subtle alterations in the activation status of microglial cells that may reshape neuronal synapses. The use of multiplex flow cytometry-based approach called flow synaptometry detected a specific dysbalance in glutamatergic synapse transmission upon H1N1 infection in mice.

MBIO (2021)

添加到收藏夹

Article Multidisciplinary Sciences

Control of lysosomal-mediated cell death by the pH-dependent calcium channel RECS1

Philippe Pihan, Fernanda Lisbona, Janina Borgonovo, Sandra Edwards-Jorquera, Paula Nunes-Hasler, Karen Castillo, Oliver Kepp, Hery Urra, Suvi Saarnio, Helena Vihinen, Amado Carreras-Sureda, Sabrina Forveille, Allan Sauvat, Daniela De Giorgis, Amaury Pupo, Diego A. Rodriguez, Giovanni Quarato, Alfredo Sagredo, Fernanda Lourido, Anthony Letai, Ramon Latorre, Guido Kroemer, Nicolas Demaurex, Eija Jokitalo, Miguel L. Concha, Alvaro Glavic, Douglas R. Green, Claudio Hetz

Summary: RECS1, a member of the TMBIM family, acts as a proapoptotic component by inducing cell death through lysosomes. It regulates lysosomal acidification and calcium content, triggering lysosomal membrane permeabilization leading to cell death. Additionally, RECS1 sensitizes whole animals to stress in Drosophila melanogaster and zebrafish models, highlighting its role in apoptosis.

SCIENCE ADVANCES (2021)

添加到收藏夹

Article Multidisciplinary Sciences

Mapping genomic loci implicates genes and synaptic biology in schizophrenia

Vassily Trubetskoy, Antonio F. Pardinas, Ting Qi, Georgia Panagiotaropoulou, Swapnil Awasthi, Tim B. Bigdeli, Julien Bryois, Chia-Yen Chen, Charlotte A. Dennison, Lynsey S. Hall, Max Lam, Kyoko Watanabe, Oleksandr Frei, Tian Ge, Janet C. Harwood, Frank Koopmans, Sigurdur Magnusson, Alexander L. Richards, Julia Sidorenko, Yang Wu, Jian Zeng, Jakob Grove, Minsoo Kim, Zhiqiang Li, Georgios Voloudakis, Wen Zhang, Mark Adams, Ingrid Agartz, Elizabeth G. Atkinson, Esben Agerbo, Mariam Al Eissa, Margot Albus, Madeline Alexander, Behrooz Z. Alizadeh, Koksal Alptekin, Thomas D. Als, Farooq Amin, Volker Arolt, Manuel Arrojo, Lavinia Athanasiu, Maria Helena Azevedo, Silviu A. Bacanu, Nicholas J. Bass, Martin Begemann, Richard A. Belliveau, Judit Bene, Beben Benyamin, Sarah E. Bergen, Giuseppe Blasi, Julio Bobes, Stefano Bonassi, Alice Braun, Rodrigo Affonseca Bressan, Evelyn J. Bromet, Richard Bruggeman, Peter F. Buckley, Randy L. Buckner, Jonas Bybjerg-Grauholm, Wiepke Cahn, Murray J. Cairns, Monica E. Calkins, Vaughan J. Carr, David Castle, Stanley Catts, Kimberley D. Chambert, Raymond C. K. Chan, Boris Chaumette, Wei Cheng, Eric F. C. Cheung, Siow Ann Chong, David Cohen, Angele Consoli, Quirino Cordeiro, Javier Costas, Charles Curtis, Michael Davidson, Kenneth L. Davis, Lieuwe de Haan, Franziska Degenhardt, Lynn E. DeLisi, Ditte Demontis, Faith Dickerson, Dimitris Dikeos, Timothy Dinan, Srdjan Djurovic, Jubao Duan, Giuseppe Ducci, Frank Dudbridge, Johan G. Eriksson, Lourdes Fananas, Stephen Faraone, Alessia Fiorentino, Andreas Forstner, Josef Frank, Nelson B. Freimer, Menachem Fromer, Alessandra Frustaci, Ary Gadelha, Giulio Genovese, Elliot S. Gershon, Marianna Giannitelli, Ina Giegling, Paola Giusti-Rodriguez, Stephanie Godard, Jacqueline Goldstein, Javier Gonzalez Penas, Ana Gonzalez-Pinto, Srihari Gopal, Jacob Gratten, Michael F. Green, Tiffany A. Greenwood, Olivier Guillin, Sinan Guloksuz, Raquel E. Gur, Ruben C. Gur, Blanca Gutierrez, Eric Hahn, Hakon Hakonarson, Vahram Haroutunian, Annette M. Hartmann, Carol Harvey, Caroline Hayward, Frans A. Henskens, Stefan Herms, Per Hoffmann, Daniel P. Howrigan, Masashi Ikeda, Conrad Iyegbe, Inge Joa, Antonio Julia, Anna K. Kahler, Tony Kam-Thong, Yoichiro Kamatani, Sena Karachanak-Yankova, Oussama Kebir, Matthew C. Keller, Brian J. Kelly, Andrey Khrunin, Sung-Wan Kim, Janis Klovins, Nikolay Kondratiev, Bettina Konte, Julia Kraft, Michiaki Kubo, Vaidutis Kucinskas, Zita Ausrele Kucinskiene, Agung Kusumawardhani, Hana Kuzelov A-Ptackova, Stefano Landi, Laura C. Lazzeroni, Phil H. Lee, Sophie E. Legge, Douglas S. Lehrer, Rebecca Lencer, Bernard Lerer, Miaoxin Li, Jeffrey Lieberman, Gregory A. Light, Svetlana Limborska, Chih-Min Liu, Jouko Lonnqvist, Carmel M. Loughland, Jan Lubinski, Jurjen J. Luykx, Amy Lynham, Milan Macek, Andrew Mackinnon, Patrik K. E. Magnusson, Brion S. Maher, Wolfgang Maier, Dolores Malaspina, Jacques Mallet, Stephen R. Marder, Sara Marsal, Alicia R. Martin, Lourdes Martorell, Manuel Mattheisen, Robert W. McCarley, Colm McDonald, John J. McGrath, Helena Medeiros, Sandra Meier, Bela Melegh, Ingrid Melle, Raquelle Mesholam-Gately, Andres Metspalu, Patricia T. Michie, Lili Milani, Vihra Milanova, Marina Mitjans, Espen Molden, Esther Molina, Maria Dolores Molto, Valeria Mondelli, Carmen Moreno, Christopher P. Morley, Gerard Muntane, Kieran C. Murphy, Inez Myin-Germeys, Igor Nenadic, Gerald Nestadt, Liene Nikitina-Zake, Cristiano Noto, Keith H. Nuechterlein, Niamh Louise O'Brien, F. Anthony O'Neill, Sang-Yun Oh, Ann Olincy, Vanessa Kiyomi Ota, Christos Pantelis, George N. Papadimitriou, Mara Parellada, Tiina Paunio, Renata Pellegrino, Sathish Periyasamy, Diana O. Perkins, Bruno Pfuhlmann, Olli Pietilainen, Jonathan Pimm, David Porteous, John Powell, Diego Quattrone, Digby Quested, Allen D. Radant, Antonio Rampino, Mark H. Rapaport, Anna Rautanen, Abraham Reichenberg, Cheryl Roe, Joshua L. Roffman, Julian Roth, Matthias Rothermundt, Bart P. F. Rutten, Safaa Saker-Delye, Veikko Salomaa, Julio Sanjuan, Marcos Leite Santoro, Adam Savitz, Ulrich Schall, Rodney J. Scott, Larry J. Seidman, Sally Isabel Sharp, Jianxin Shi, Larry J. Siever, Engilbert Sigurdsson, Kang Sim, Nora Skarabis, Petr Slominsky, Hon-Cheong So, Janet L. Sobell, Erik Soderman, Helen J. Stain, Nils Eiel Steen, Agnes A. Steixner-Kumar, Elisabeth Stogmann, William S. Stone, Richard E. Straub, Fabian Streit, Eric Strengman, T. Scott Stroup, Mythily Subramaniam, Catherine A. Sugar, Jaana Suvisaari, Dragan M. Svrakic, Neal R. Swerdlow, Jin P. Szatkiewicz, Thi Minh Tam Ta, Atsushi Takahashi, Chikashi Terao, Florence Thibaut, Draga Toncheva, Paul A. Tooney, Silvia Torretta, Sarah Tosato, Gian Battista Tura, Bruce Turetsky, Alp Ucok, Arne Vaaler, Therese van Amelsvoort, Ruud van Winkel, Juha Veijola, John Waddington, Henrik Walter, Anna Waterreus, Bradley T. Webb, Mark Weiser, Nigel M. Williams, Stephanie H. Witt, Brandon K. Wormley, Jing Qin Wu, Zhida Xu, Robert Yolken, Clement C. Zai, Wei Zhou, Feng Zhu, Fritz Zimprich, Muhammad Ayub, Christian Benner, Alessandro Bertolino, Donald W. Black, Nicholas J. Bray, Gerome Breen, Nancy G. Buccola, William F. Byerley, Wei J. Chen, C. Robert Cloninger, Benedicto Crespo-Facorro, Gary Donohoe, Robert Freedman, Cherrie Galletly, Michael J. Gandal, Massimo Gennarelli, David M. Hougaard, Hai-Gwo Hwu, Assen Jablensky, Steven A. McCarroll, Jennifer L. Moran, Ole Mors, Preben B. Mortensen, Bertram Muller-Myhsok, Amanda L. Neil, Merete Nordentoft, Michele T. Pato, Tracey L. Petryshen, Matti Pirinen, Ann E. Pulver, Thomas G. Schulze, Jeremy M. Silverman, Jordan W. Smoller, Eli A. Stahl, Debby W. Tsuang, Elisabet Vilella, Shi-Heng Wang, Shuhua Xu, Rolf Adolfsson, Celso Arango, Bernhard T. Baune, Sintia Iole Belangero, Anders D. Borglum, David Braff, Elvira Bramon, Joseph D. Buxbaum, Dominique Campion, Jorge A. Cervilla, Sven Cichon, David A. Collier, Aiden Corvin, David Curtis, Marta Di Forti, Enrico Domenici, Hannelore Ehrenreich, Valentina Escott-Price, Tonu Esko, Ayman H. Fanous, Anna Gareeva, Micha Gawlik, Pablo Gejman, Michael Gill, Stephen J. Glatt, Vera Golimbet, Kyung Sue Hong, Christina M. Hultman, Steven E. Hyman, Nakao Iwata, Erik G. Jonsson, Rene S. Kahn, James L. Kennedy, Elza Khusnutdinova, George Kirov, James A. Knowles, Marie-Odile Krebs, Claudine Laurent-Levinson, Jimmy Lee, Todd Lencz, Douglas F. Levinson, Qingqin S. Li, Jianjun Liu, Anil K. Malhotra, Dheeraj Malhotra, Andrew McIntosh, Andrew McQuillin, Paulo R. Menezes, Vera A. Morgan, Derek W. Morris, Bryan J. Mowry, Robin M. Murray, Vishwajit Nimgaonkar, Markus M. Nothen, Roel A. Ophoff, Sara A. Paciga, Aarno Palotie, Carlos N. Pato, Shengying Qin, Marcella Rietschel, Brien P. Riley, Margarita Rivera, Dan Rujescu, Meram C. Saka, Alan R. Sanders, Sibylle G. Schwab, Alessandro Serretti, Pak C. Sham, Yongyong Shi, David St Clair, Hreinn Stefansson, Kari Stefansson, Ming T. Tsuang, Jim van Os, Marquis P. Vawter, Daniel R. Weinberger, Thomas Werge, Dieter B. Wildenauer, Xin Yu, Weihua Yue, Peter A. Holmans, Andrew J. Pocklington, Panos Roussos, Evangelos Vassos, Matthijs Verhage, Peter M. Visscher, Jian Yang, Danielle Posthuma, Ole A. Andreassen, Kenneth S. Kendler, Michael J. Owen, Naomi R. Wray, Mark J. Daly, Hailiang Huang, Benjamin M. Neale, Patrick F. Sullivan, Stephan Ripke, James T. R. Walters, Michael C. O'Donovan

Summary: In this study, a two-stage genome-wide association study was conducted to identify common variants associated with schizophrenia. The results revealed 287 distinct genomic loci and 120 genes likely to be involved in the development of schizophrenia. This research provides valuable insights into the pathophysiology of schizophrenia and offers a resource for further mechanistic studies.

NATURE (2022)

添加到收藏夹

Article Multidisciplinary Sciences

Engineering an acetoacetyl-CoA reductase from Cupriavidus necator toward NADH preference under physiological conditions

Karel Olavarria, Yared O. Pijman, Ricardo Cabrera, Mark C. M. van Loosdrecht, S. Aljoscha Wahl

Summary: The cofactor preference of the acetoacetyl-CoA reductase encoded by the phaB1 gene from Cupriavidus necator was studied in relation to PHB accumulation.

SCIENTIFIC REPORTS (2022)

添加到收藏夹

Review Pharmacology & Pharmacy

Bisphosphonates Targeting Ion Channels and Musculoskeletal Effects

Rosa Scala, Fatima Maqoud, Marina Antonacci, Jacopo Raffaele Dibenedetto, Maria Grazia Perrone, Antonio Scilimati, Karen Castillo, Ramon Latorre, Diana Conte, Said Bendahhou, Domenico Tricarico

Summary: Bisphosphonates are commonly used bone-specific anti-resorptive agents, but their effects on ion channels in musculoskeletal cells are poorly understood. Ion channels are emerging as potential targets for bisphosphonates, particularly the TRPV1 channel in osteoblasts. Further research in this area could provide insights into new therapeutic approaches for skeletal disorders.

FRONTIERS IN PHARMACOLOGY (2022)

添加到收藏夹

Article Cell Biology

TRPV4 acts as a mitochondrial Ca2+-importer and regulates mitochondrial temperature and metabolism

Tusar Kanta Acharya, Ashutosh Kumar, Rakesh Kumar Majhi, Shamit Kumar, Ranabir Chakraborty, Ankit Tiwari, Karl-Heinz Smalla, Xiao Liu, Young-Tae Chang, Eckart D. Gundelfinger, Chandan Goswami

Summary: TRPV4 is associated with various diseases, and mitochondrial abnormalities play a significant role in these diseases. Improper expression and function of TRPV4 lead to mitochondrial abnormalities, and TRPV4 also regulates mitochondrial numbers, Ca2+ levels, and temperature. Naturally occurring TRPV4 mutations affect mitochondrial morphology and distribution, which helps in understanding TRPV4-mediated channelopathies and classifying them as mitochondrial diseases.

MITOCHONDRION (2022)

添加到收藏夹

Article Biology

Transcriptional diversity in specific synaptic gene sets discriminates cortical neuronal identity

Amparo Roig Adam, Jose A. Martinez-Lopez, Sophie J. F. van der Spek, Patrick F. Sullivan, August B. Smit, Matthijs Verhage, Jens Hjerling-Leffler, Tilmann Achsele, Maria Andres-Alonso, Claudia Bagni, Alex Bayes, Thomas Biederer, Nils Brose, John Jia En Chua, Marcelo Coba, L. Niels Cornelisse, Jaime de Juan-Sanz, Hana L. Goldschmidt, Eckart D. Gundelfinger, Richard L. Huganir, Cordelia Imig, Reinhard Jahn, Hwajin Jung, Pascal S. Kaeser, Eunjoon Kim, Frank Koopmans, Michael R. Kreutz, Noa Lipstein, Harold D. MacGillavry, Peter S. McPherson, Vincent O'Connor, Rainer Pielot, Timothy A. Ryan, Carlo Sala, Morgan Sheng, Karl-Heinz Smalla, Paul D. Thomas, Ruud F. Toonen, Jan R. T. van Weering, Matthijs Verhage, Chiara Verpelli

Summary: Synapse diversity has been described from different perspectives, ranging from the specific neurotransmitters released, to their diverse biophysical properties and proteome profiles. However, synapse diversity at the transcriptional level has not been systematically identified across all synapse populations in the brain. To quantify and identify specific synaptic features of neuronal cell types, the SynGO database was combined with single-cell RNA sequencing data of the mouse neocortex. This study provides a deeper understanding of synapse diversity in the neocortex and identifies potential markers to selectively identify synapses from specific neuronal populations.

BIOLOGY DIRECT (2023)

添加到收藏夹

Article Biochemistry & Molecular Biology

Extracellular Matrix Changes in Subcellular Brain Fractions and Cerebrospinal Fluid of Alzheimer's Disease Patients

Lukas Hoehn, Wilhelm Hussler, Anni Richter, Karl-Heinz Smalla, Anna-Maria Birkl-Toeglhofer, Christoph Birkl, Stefan Vielhaber, Stefan L. Leber, Eckart D. Gundelfinger, Johannes Haybaeck, Stefanie Schreiber, Constanze I. Seidenbecher

Summary: The study investigates changes in the brain's extracellular matrix (ECM) in Alzheimer's disease (AD) and finds spatially segregated molecular rearrangements of the ECM at RNA or protein levels.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)

添加到收藏夹

Article Biochemistry & Molecular Biology

The many Neurofaces of Prohibitins 1 and 2: Crucial for the healthy brain, dysregulated in numerous brain disorders

Hans-Gert Bernstein, Karl-Heinz Smalla, Gerburg Keilhoff, Henrik Dobrowolny, Michael R. Kreutz, Johann Steiner

Summary: Prohibitin 1 (PHB1) and prohibitin 2 (PHB2) are widely expressed proteins localized in mitochondria, cytosol, and cell nuclei. They play important roles in brain development, aging, metabolism regulation, structural integrity maintenance, synapse formation, neurotransmission, and the action of hypothalamic-pituitary hormones. However, they are also involved in various major diseases of the CNS, for which there is currently no targeted therapy available.

JOURNAL OF CHEMICAL NEUROANATOMY (2023)

添加到收藏夹

Meeting Abstract Biophysics

The bisphosphonate zoledronic acid is a TRPV1 channel inhibitor

Karen Castillo, Yorley Duarte, Ignacio A. Segura, Rodrigo Meza, Carlos Ancaten, Karina Carvajal-Zamorano, Rosa Scala, Domenico Tricarico, Andres Chavez, Ramon Latorre

BIOPHYSICAL JOURNAL (2023)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Design and thermodynamic analysis of a pathway enabling anaerobic production of poly-3-hydroxybutyrate in Escherichia coli

Karel Olavarria, Marco V. Becker, Diana Z. Sousa, Mark C. M. van Loosdrecht, S. Aljoscha Wahl

Summary: This study proposes a novel metabolic engineering strategy to achieve efficient anaerobic production of poly-(R)-3-hydroxybutyrate (PHB) in Escherichia coli. The strategy involves rerouting the glycolytic flux through non-oxidative glycolysis and coupling PHB synthesis with NADH re-oxidation. Stoichiometric analysis and various thermodynamic approaches were used to assess the feasibility and bottlenecks of the engineered pathway.

SYNTHETIC AND SYSTEMS BIOTECHNOLOGY (2023)

添加到收藏夹

暂无数据

© Peeref 2019-2024. All rights reserved.