期刊
NEUROPHARMACOLOGY
卷 82, 期 -, 页码 49-58出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuropharm.2014.03.007
关键词
Lateral amygdala; Serotonin; TRPC; GIRK; PIP2; PI3-kinase
资金
- Japan Society for the Promotion of Science [25870854]
- Kanazawa Medical University (Promoted Research) [S2013-14]
- Naito Foundation
- Casio Science Promotion Foundation
- Grants-in-Aid for Scientific Research [25870854] Funding Source: KAKEN
Regional differences are known in the serotonin-induced modulation of neuronal activity within the amygdala. This in vitro study in rats focuses on analyzing the ionic mechanism underlying serotonin-induced depolarization in the lateral amygdala. Serotonin depolarized membrane potential by 5 mV, which is underlain by a serotonin-induced inward current at rest with a characteristic reversal potential of -105 mV. From pharmacological experiments, the 5-HT2C subtype was singled out as the receptor subtype involved. Under blockade of K+ channels by Ba2+, 5-HT induced an inward current with no reversal at the range between -50 and -130 mV, which was identified as a TRPC-like current This current was blocked by the specific phosphatidylinositol 3-kinse (PI3-kinase) inhibitor LY294002, pointing to its dependence on PI3-kinase. The Ba2+-sensitive component, obtained by subtraction, showed a strong outward rectification and the reversal potential of K+, indicating that this component results from a serotonin-induced inhibition of G-protein coupled inwardly rectifying K+ channel (GIRK) current By wortmannin, an inhibitor of both PI3-kinase and PI4-kinase, a serotonin-induced phosphatidylinositol 4,5-bisphosphate (PIP2) depletion was revealed to underlie GIRK inhibition. Thus, the serotonin-induced current turned out to be caused by a combined occurrence of GIRK inhibition and PI3-kinase-dependent TRPC-like current. With serotonergic modulation, all these mechanisms should be recruited in lateral amygdala principal neurons and likely contribute to generation of region-specific neuronal activity patterns within the amygdala, which may at least partly implement its required role in fear and anxiety. (C) 2014 Elsevier Ltd. All rights reserved.
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