期刊
NEUROPHARMACOLOGY
卷 63, 期 7, 页码 1218-1226出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuropharm.2012.07.043
关键词
Synaptic transmission; K+ channels; GABA receptor; Hippocampus; G-protein; Synapse
资金
- National Institutes of Mental Health [MH082881]
Whereas vasopressin has been shown to enhance memory possibly by increasing long-term potentiation and direct excitation of the pyramidal neurons in the hippocampus, the effects of vasopressin on GABAergic transmission in the hippocampus remain to be determined. Here we examined the effects of vasopressin on GABAergic transmission onto CA1 pyramidal neurons and our results demonstrate that bath application of [Arg(8)]-vasopressin (AVP) dose-dependently increased the frequency of spontaneous IPSCs (sIPSCs) recorded from CA1 pyramidal neurons via activation of V-1A receptors. Immunohistological staining and western blot further confirmed that both CA1 pyramidal neurons and interneurons expressed V-1A receptors. Bath application of AVP altered neither the frequency nor the amplitude of miniature IPSCs in the presence of tetradotoxin and failed to change significantly the amplitude of evoked IPSCs recorded from CA1 pyramidal neurons. AVP increased the firing frequency of action potentials by depolarizing the GABAergic interneurons in the stratum radiatum of CA1 region. AVPmediated depolarization of interneurons was mediated by inhibition of a background K+ conductance which was insensitive to extracellular tetraethylammonium, Cs+, 4-aminopyridine, tertiapin-Q and Ba2+. AVP-induced depolarization of interneurons was dependent on Gaol but independent of phospholipase C, intracellular Ca2+ release and protein kinase C. The inhibitory effects of AVP-mediated modulation of GABA release onto CA1 pyramidal neurons were overwhelmed by its strong excitation of CA1 pyramidal neurons in physiological condition but revealed when its direct excitation of the pyramidal neurons was blocked suggesting that AVP-mediated modulation of GABAergic transmission fine-tunes the excitability of CA1 pyramidal neurons. (c) 2012 Elsevier Ltd. All rights reserved.
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