Review
Neurosciences
Jithin D. Nair, Kevin A. Wilkinson, Jeremy M. Henley, Jack R. Mellor
Summary: This article reviews the roles of kainate receptors in synaptic plasticity, focusing on their impact on postsynaptic signaling and presynaptic auto-receptor functions. It also explores the plasticity of kainate receptors themselves and their contribution to synaptic transmission plasticity.
Article
Biochemistry & Molecular Biology
Rafael Falcon-Moya, Irene Martinez-Gallego, Antonio Rodriguez-Moreno
Summary: KARs in the CA2 region of the hippocampus mediate the depression of glutamate release through a mechanism involving the activation of G protein and protein kinase A.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Rajesh Vinnakota, Surbhi Dhingra, Jyoti Kumari, Mohammed Yousuf Ansari, Ekta Shukla, Mayuri Dattatray Nerkar, Janesh Kumar
Summary: This study characterized the biochemical, biophysical, and functional properties of the purified extracellular domain of Neto1, revealing a conformation-dependent interaction with GluK2 receptors. Furthermore, the shape and conformation of Neto1-ECD seem to be altered by calcium ions, indicating a possible role in modulating Neto1 functions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Biochemistry & Molecular Biology
Deepa Gautam, Ulhas P. Naik, Meghna U. Naik, Santosh K. Yadav, Rameshwar Nath Chaurasia, Debabrata Dash
Summary: This paper explores the role of platelets in neurodegenerative disorders such as Alzheimer's disease and Parkinson's disease, as well as the critical role of glutamate receptors in the pathophysiology of these diseases. By reviewing data from clinical trials, animal models, and cellular studies, it reveals the involvement of glutamate receptor dysfunction in neurodegenerative processes and provides an overview of current treatments, including glutamate receptor antagonists, for AD and PD.
Article
Neurosciences
Fumeng Zheng, Haruyuki Kamiya
Summary: Modification of axonal excitability affects information transfer in the brain, but the functional significance of this modulation remains unclear. This study focused on the activity-dependent broadening of action potentials in the hippocampal mossy fibers and found that it is dependent on the inactivation of axonal K+ channels. Computer simulations showed that removing the inactivation process abolished the use-dependent broadening of action potentials. This highlights the critical role of K+ channel inactivation in regulating axonal excitability and short-term plasticity at this synapse.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Neurosciences
Simo Ojanen, Tatiana Kuznetsova, Zoia Kharybina, Vootele Voikar, Sari E. E. Lauri, Tomi Taira
Summary: The absence of GluK1 containing KARs in GABAergic interneurons disrupts GABAergic neurotransmission and spontaneous network activity in the neonatal hippocampus, while leading to stronger hippocampal gamma oscillations and enhanced theta-gamma cross frequency coupling in adult male mice. In females, loss of interneuronal GluK1 results in shorter sharp wave ripple oscillations and slightly impaired abilities in flexible sequencing tasks.
Review
Neurosciences
Jeremy M. Henley, Jithin D. Nair, Richard Seager, Busra P. Yucel, Gavin Woodhall, Benjamin S. Henley, Karolina Talandyte, Hope I. Needs, Kevin A. Wilkinson
Summary: Epilepsy is caused by abnormal neuronal network activity. The review focuses on the role and regulation of kainate and AMPA receptors in seizure activity, discussing the impact of GluK2 and GluA2 subunits on their respective receptors' properties.
Article
Cell Biology
Anu G. Nair, Paola Muttathukunnel, Martin Mueler
Summary: The study demonstrates that different receptor perturbations at the Drosophila neuromuscular junction result in different responses, with some receptor inhibitions not compensated by PHP while others induce compensatory PHP through distinct signaling pathways. This highlights the diversity of homeostatic signaling mechanisms, showing that synapses respond differentially to similar activity impairments and achieve homeostatic compensation via different pathways.
Article
Multidisciplinary Sciences
Shuo Han, Xiang-Ping Chu, Ryan Goodson, Prae Gamel, Sophia Peng, Joshua Vance, Shizhen Wang
Summary: Cholesterol inhibits the hHv1 channel by stabilizing the voltage-sensing S4 segment at resting conformations, providing a mechanism for the regulation of fertilization by the hHv1 channel.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Agriculture, Multidisciplinary
Benjie Li, Ying Yan, Guangkai Yao, Ling Zhang, Fei Lin, Hanhong Xu
Summary: A series of novel pyrazoloquinazolines were synthesized, and pyrazoloquinazoline 5a was found to have high insecticidal activity against the diamondback moth without cross-resistance to fipronil. Electrophysiological experiments suggested that 5a acts on the GABAR and GluCl. The potency of 5a on PxGluCl was 15-fold higher than fipronil, explaining the lack of cross-resistance. Downregulation of PxGluCl transcription significantly enhanced the insecticidal activity of 5a on the diamondback moth.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Kento Ojima, Kazuki Shiraiwa, Kyohei Soga, Tomohiro Doura, Mikiko Takato, Kazuhiro Komatsu, Michisuke Yuzaki, Itaru Hamachi, Shigeki Kiyonaka
Summary: The method presented in the study combines affinity-based receptor labeling and bioorthogonal click chemistry to quantify AMPAR distribution and trafficking under physiological conditions. This versatile tool is successfully expanded to selectively label NMDA-type glutamate receptors.
NATURE COMMUNICATIONS
(2021)
Article
Neurosciences
Thomas M. Sanderson, Liam T. Ralph, Mascia Amici, Ai Na Ng, Bong-Kiun Kaang, Min Zhuo, Sang Jeong Kim, John Georgiou, Graham L. Collingridge
Summary: In hippocampal area CA1, mGluR-LTD can be expressed by a decrease in neurotransmitter release probability or a change in postsynaptic AMPA receptor number. The determinant of expression locus is still unknown. Using different concentrations of DHPG, we found that low concentration DHPG induces presynaptic LTD that requires NMDA receptor co-activation, while high concentration DHPG induces postsynaptic LTD that is independent of NMDA receptor activation. Our results reveal an unexpected complexity to mGluR-mediated synaptic plasticity in the hippocampus.
FRONTIERS IN SYNAPTIC NEUROSCIENCE
(2022)
Review
Pharmacology & Pharmacy
Anna Pittaluga
Summary: This review summarizes the results from synaptosome studies over the past 40 years, supporting the existence of auto and hetero NMDA receptors controlling the release of transmitters in the CNS of mammals. Additionally, immunochemical studies in isolated nerve endings confirm these functional observations.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lisa Klotz, Ralf Enz
Summary: The study analyzed the expression and localization of mGluR7a and mGluR7b in mouse cochlear wholemounts, finding a presynaptic localization of mGluR7a at inner hair cells (IHCs) and co-localization of mGluR7b with mGluR7a at IHC ribbon synapses. The numbers of mGluR7a and mGluR7b puncta were reduced at higher frequencies and in older animals, indicating potential age and frequency-related changes in the cochlea.
Article
Neurosciences
Anamitra Ghosh, Sangu Muthuraju, Sean Badal, Jessica Wooden, J. Leigh Leasure, Gregg Roman, Joydip Das
Summary: The expression of Munc13-1 and Munc13-2 in the hippocampus is differentially affected by alcohol consumption, and both Munc13-1 and Munc13-2 may contribute to the ethanol-induced augmentation of glutamatergic neurotransmission.
Article
Multidisciplinary Sciences
Benjamin Compans, Come Camus, Emmanouela Kallergi, Silvia Sposini, Magalie Martineau, Corey Butler, Adel Kechkar, Remco Klaassen, Natacha Retailleau, Terrence J. Sejnowski, August B. Smit, Jean-Baptiste Sibarita, Thomas M. Bartol, David Perrais, Vassiliki Nikoletopoulou, Daniel Choquet, Eric Hosy
Summary: Long-term depression (LTD) of synaptic strength is associated with circuit remodeling, memory encoding, and erasure. P2XR- and NMDAR-dependent LTD are linked to distinct molecular modifications that lead to specific changes in synapse function.
NATURE COMMUNICATIONS
(2021)
Article
Clinical Neurology
Tomas Jorda-Siquier, Melina Petrel, Vladimir Kouskoff, Una Smailovic, Fabrice Cordelieres, Susanne Frykman, Ulrike Mueller, Christophe Mulle, Gael Barthet
Summary: The distribution of amyloid precursor protein (APP) and its fragments is altered in Alzheimer's disease (AD), leading to their accumulation around amyloid plaques with presynaptic proteins. This finding is associated with histopathological features and familial AD.
ALZHEIMERS & DEMENTIA
(2022)
Article
Neurosciences
Adam Gorlewicz, Gael Barthet, Stefano Zucca, Peggy Vincent, Marilena Griguoli, Noelle Grosjean, Grzegorz Wilczynski, Christophe Mulle
Summary: Kainate receptors (KARs) play a crucial role in regulating synaptic circuits through ionotropic or metabotropic mechanisms. This study reveals that the acute convulsive effect of kainate is primarily dependent on GluK2/GluK5 containing KARs, while the convulsive activity induced by pilocarpine and pentylenetetrazol is not affected by the absence of KARs. Interestingly, the genetic inactivation of GluK2 increases susceptibility to acute pilocarpine-induced seizures.
Article
Biochemistry & Molecular Biology
Christian B. M. Poulie, Younes Larsen, Cindie Leteneur, Gael Barthet, Walden E. Bjorn-Yoshimoto, Fanny Malhaire, Birgitte Nielsen, Jean-Phillippe Pin, Christophe Mulle, Darryl S. Pickering, Lennart Bunch
Summary: This study reports the pharmacological characterization of (S)-2-hydroxyhistidine (2a) and (S)-2-mercaptohistidine (2b) as mediators of glutamatergic neurotransmission. It was found that 2a showed no binding affinity or activity at all glutamate receptors and transporters, while 2b displayed selectivity for homomeric GluK3 receptors.
ACS CHEMICAL NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Michael H. Raymond, Andrew J. Davidson, Yi Shen, Daniel R. Tudor, Christopher D. Lucas, Sho Morioka, Justin S. A. Perry, Julia Krapivkina, David Perrais, Linus J. Schumacher, Robert E. Campbell, Will Wood, Kodi S. Ravichandran
Summary: By utilizing the CharON reporter, it was found that in the embryonic development of Drosophila, emerging apoptotic cells are cleared by macrophages, but some macrophages may carry a high corpse burden, affecting their ability to clear wound debris.
Article
Multidisciplinary Sciences
Vincent Paget-Blanc, Marlene E. Pfeffer, Marie Pronot, Paul Lapios, Maria-Florencia Angelo, Roman Walle, Fabrice P. Cordelieres, Florian Levet, Stephane Claverol, Sabrina Lacomme, Melina Petrel, Christelle Martin, Vincent Pitard, Veronique De Smedt Peyrusse, Thomas Biederer, David Perrais, Pierre Trifilieff, Etienne Herzog
Summary: Dopamine is an important regulator of brain function, and its transmission at dopamine hub synapses may synergize with other neurotransmitters. This study used targeted labeling and fluorescence activated sorting to purify striatal dopaminergic synaptosomes and identified 57 specifically enriched proteins. Additionally, the adhesion of dopaminergic synapses to glutamatergic, GABAergic, or cholinergic synapses was observed, supporting local dopaminergic signaling.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Domenico Azarnia Tehran, Gaga Kochlamazashvili, Niccolo P. Pampaloni, Silvia Sposini, Jasmeet Kaur Shergill, Martin Lehmann, Natalya Pashkova, Claudia Schmidt, Delia Loewe, Hanna Napieczynska, Arnd Heuser, Andrew J. R. Plested, David Perrais, Robert C. Piper, Volker Haucke, Tanja Maritzen
Summary: This study reveals that the Alzheimer's disease risk factor CALM controls the surface levels of CP-AMPARs through endocytosis, and this regulation reciprocally modulates LTP and LTD in vivo to influence learning.
Article
Multidisciplinary Sciences
Kelsie Eichel, Takeshi Uenaka, Vivek Belapurkar, Rui Lu, Shouqiang Cheng, Joseph S. Pak, Caitlin A. Taylor, Thomas C. Sudhof, Robert Malenka, Marius Wernig, Engin Ozkan, David Perrais, Kang Shen
Summary: This study reveals an important mechanism for maintaining neuronal polarity in the axon initial segment (AIS), which involves the endocytic removal of polarized receptors that diffuse into the AIS. This mechanism works together with the known AIS diffusion-barrier mechanism to reinforce the membrane boundaries between axonal and dendritic compartments.
Article
Biochemistry & Molecular Biology
Marine Laporte, Kwang Chi, Laura Caudal, Na Zhao, Yvonne Schwarz, Marta Rolland, Jose Martinez-Hernandez, Magalie Martineau, Christine Chatellard, Eric Denarier, Vincent Mercier, Florent Lemaitre, Beatrice Blot, Eve Moutaux, Maxime Cazorla, David J. Perrais, Fabien Lante, Dieter Bruns, Sandrine Fraboulet, Fiona Hemming, Frank Kirchhoff, Remy Sadoul
Summary: This study shows that Alix is involved in regulating bulk endocytosis at synapses during high frequency stimulation, and impairment of this process due to the lack of Alix results in abnormal synaptic recovery and plasticity.
Review
Biochemistry & Molecular Biology
David Perrais
Summary: Dynamin is a major protein involved in endocytosis and plays a central role in cellular processes such as actin remodeling. It has been extensively studied through various methods and has been shown to be a remarkable mechano-GTPase. It is part of a complex protein and lipid network in cells and cannot function in isolation.
BIOSCIENCE REPORTS
(2022)
Article
Neurosciences
Gael Barthet, Ana Moreira-de-Sa, Pei Zhang, Jorge Castanheira, Adam Gorlewicz, Christophe Mulle
Summary: Kainate receptors (KARs) play a role in regulating neuronal networks and have been implicated in epilepsy. This study investigates the role of KARs in Alzheimer's disease (AD) and finds that the synaptic expression and function of KARs are impaired in mouse models of AD. The study also suggests that the protein APP stabilizes KARs at synapses through a transsynaptic mechanism, and this interaction is regulated by the c-secretase proteolytic activity of presenilin.
JOURNAL OF NEUROSCIENCE
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Domenico Azarnia Tehran, Gaga Kochlamazashvili, Niccolo P. Pampaloni, Silvia Sposini, Jasmeet Kaur Shergill, Martin Lehmann, Natalya Pashkova, Claudia Schmidt, Delia Loewe, Hanna Napieczynska, Arnd Heuser, Andrew J. R. Plested, David Perrais, Robert C. Piper, Volker Haucke, Tanja Maritzen
JOURNAL OF NEUROCHEMISTRY
(2023)
Meeting Abstract
Cell Biology
K. Eichel, V. Belapurkar, D. Perrais, K. Shen
MOLECULAR BIOLOGY OF THE CELL
(2023)
Meeting Abstract
Cell Biology
K. Eichel, V. Belapurkar, D. Perrais, K. Shen
MOLECULAR BIOLOGY OF THE CELL
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
R. Baccouch, M. Jobin, V. De Smedt-Peyrousse, R. Guixa-Gonzalez, T. Durroux, D. Perrais, P. Trifilieff, I. Alves
Correction
Neurosciences
Lucia Privitera, Ellen L. Hogg, Matthias Gaestel, Mark J. Wall, Sonia A. L. Correa
Article
Neurosciences
Li-Ya Jiang, Guan-Hao Wang, Jing-Jiao Xu, Xiao-Li Li, Xiao-Yan Lin, Xiang Fang, Hong-Xu Zhang, Mei Feng, Chun-Ming Jiang
Summary: This study reveals the importance of LINC00473 in regulating temozolomide (TMZ) resistance in glioblastoma (GB) and its potential mechanism. By regulating the expression of CEBP alpha and MGMT, LINC00473 promotes the formation of chemoresistance. Furthermore, LINC00473 can transfer chemoresistance to adjacent sensitive cells through exosomes.
Article
Neurosciences
Olga Kopach, Tetyana Pivneva, Nataliya Fedirko, Nana Voitenko
Summary: This study found that diabetic animals exhibit severe xerostomia characterized by reduced saliva flow rate, diminished total protein content, and decreased amylase activity. The impaired saliva production in diabetes is associated with reduced and delayed intracellular Ca2+ signals in submandibular acinar cells, caused by malfunctioning mitochondria. Targeting malfunctioning mitochondria may be a potential strategy for the treatment of diabetic xerostomia.
Article
Neurosciences
Nicholas M. Timme, Cherish E. Ardinger, Seth D. C. Weir, Rachel Zelaya-Escobar, Rachel Kruger, Christopher C. Lapish
Summary: This study aimed to assess aversion-resistant drinking behavior in head-fixed mice and explore the relationship between non-consummatory behaviors and aversion-resistant drinking. The results showed that head-fixed mice exhibited heterogenous levels of aversion-resistant drinking and non-consummatory behaviors were related to the intensity of this behavior.
Article
Neurosciences
David R. Maguire, Charles P. France
Summary: Methocinnamox (MCAM) is a novel, long-acting opioid receptor antagonist that effectively decreases fentanyl self-administration and prevents opioid overdose in monkeys. The study demonstrates the potential therapeutic utility of MCAM in the treatment of opioid use disorder.
Article
Neurosciences
Xiang Li, Dan Feng, Shenglu Ma, Mingxing Li, Shulei Zhao, Man Tang
Summary: This study investigated the effects of fluoxetine on neurochemical, neurobiological, and neurobehavioral changes in different subregions of the hippocampus. The results showed that fluoxetine increased dialysate 5-HT, decreased membrane 5-HTT protein, and increased cytoplasmic fraction. Additionally, fluoxetine reduced immobility times in behavioral tests, with greater effects observed in the ventral subregion compared to the dorsal subregion.
Article
Neurosciences
Alexander V. Zholos, Mariia I. Melnyk, Dariia O. Dryn
Summary: Acetylcholine is an important neurotransmitter in visceral smooth muscles, activating M2 and M3 muscarinic receptors to cause smooth muscle excitation and contraction. This review focuses on the cellular and molecular mechanisms underlying acetylcholine-induced depolarisation and smooth muscle contraction, as well as the effects of anticholinergic drugs on gastrointestinal motility. The knowledge gained from recent studies has greatly expanded our understanding of these processes.
Article
Neurosciences
Zhenlong Li, Hsien-Yu Peng, Chau-Shoun Lee, Tzer-Bin Lin, Ming-Chun Hsieh, Cheng-Yuan Lai, Han-Fang Wu, Lih-Chyang Chen, Mei-Ci Chen, Dylan Chou
Summary: Methylone shows significant efficacy in treating depression and social deficits, making it an ideal candidate for anti-depressant medication.
Article
Neurosciences
Aline Freyssin, Allison Carles, Sarra Guehairia, Gilles Rubinstenn, Tangui Maurice
Summary: This study explores the potential of combining FENM and S1R agonists in the treatment of Alzheimer's disease. The results showed that most FENM-based combinations can protect against learning deficits caused by A beta 25-35, with better efficacy in short-term memory.
Article
Neurosciences
J. D. Lorente, J. Cuitavi, L. Rullo, S. Candeletti, P. Romualdi, L. Hipolito
Summary: This study analyzed the effects of pain on negative affect in different sexes and time courses, as well as the involvement of the dynorphinergic and corticotropin releasing factor systems in these pain-related behaviors. The results showed sex and time-dependent anxiety- and anhedonia-like behaviors induced by pain in female rats. The recruitment of KOR/DYN in the NAc was identified as a key neurological substrate mediating pain-induced behavioral alterations.
Article
Neurosciences
Rongjun Liu, Daofan Sun, Xiuzhong Xing, Qingge Chen, Bo Lu, Bo Meng, Hui Yuan, Lan Mo, Liufang Sheng, Jinwei Zheng, Qiusheng Wang, Junping Chen, Xiaowei Chen
Summary: The coexistence of pain and depression is frequently observed in patients with chronic pain and depression. Oxytocin, a neuropeptide, has been reported to relieve chronic pain and depressive symptoms. This study investigated the effect of intranasal oxytocin on neuropathic pain and comorbid depressive symptoms, and found that oxytocin attenuated depression-like behavior but did not alleviate mechanical hyperalgesia. The results suggest that intranasal oxytocin may have the potential to treat depressive symptoms in neuropathic pain patients.
