Article
Biochemistry & Molecular Biology
Katarzyna Terejko, Michal A. Michalowski, Ilona Izykowska, Anna Dominik, Aleksandra Brzostowicz, Jerzy W. Mozrzymas
Summary: GABA type A receptors play a crucial role in mediating inhibition in the adult mammalian brain and have a complex gating mechanism involving openings/closings, preactivation, and desensitization. The ligand binding sites affect the receptor's gating mechanism, suggesting shared structural determinants for distinct conformational transitions.
ACS CHEMICAL NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Juan Facundo Chrestia, Ariana Bruzzone, Maria del Carmen Esandi, Cecilia Bouzat
Summary: The alpha 7 nicotinic acetylcholine receptor plays a role in neurological, neurodegenerative, and inflammatory disorders, serving as both a ligand-gated cationic channel and a metabotropic receptor. Phosphorylation modulates alpha 7 channel activity and cellular response, impacting alpha 7 cholinergic signaling broadly.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biology
Crescent L. Combe, Carol M. Upchurch, Carmen C. Canavier, Sonia Gasparini
Summary: A combination of in vitro electrophysiology and modeling identified TRPM4 channels as major drivers of cholinergic modulation of firing rate in rat CA1 pyramidal neurons. Cholinergic agonist carbachol reverses the spike rate adaptation caused by Na-V channel inactivation, resulting in more spikes on the down-ramp compared to the up-ramp. Pharmacological experiments and modeling suggest that TRPM4 channels play a role in the cholinergic shift of firing center of mass through Ca2+-activated nonspecific cation current ICAN.
Article
Neurosciences
Veronika Bodi, Timea Majer, Viktor Kelemen, Ildiko Vilagi, Attila Szucs, Petra Varro
Summary: ASD is considered as one of the main causes of neurodevelopmental disorders characterized by impairments in social interactions and repetitive behavior. Prenatal VPA treatment has diverse effects on hippocampal excitability depending on the sex and age of the animals, with differences in excitability observed in male and female rats of different age groups.
FRONTIERS IN NEURAL CIRCUITS
(2022)
Article
Biochemistry & Molecular Biology
Milica Gusic, Klaus Benndorf, Christian Sattler
Summary: Our study reveals that each occupied binding site contributes to channel activation in P2X7 receptors. While the occupation of a single binding site can activate the channels, three bound ligands maximally stabilize the open state, suggesting a stepwise activation process for P2X7 receptors.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Metallurgy & Metallurgical Engineering
Lin Xin, Hu Yan-ling, Zhang Chi, Yin Jie, Cui Rong, Yang Dong-liang, Chen Bo
Summary: The study showed that the shape of gold nanoparticles (GNPs) affects the voltage-dependent sodium channel and action potential of hippocampal CA1 neurons in mice. GNPs inhibited the sodium current amplitudes and shifted the voltage-dependence curve, leading to increased neuronal excitability and altered action potential properties. Nanoflower-shaped GNPs had the most significant effects on action potential properties, indicating that the neurotoxicity of GNPs is dependent on surface roughness.
JOURNAL OF CENTRAL SOUTH UNIVERSITY
(2021)
Article
Instruments & Instrumentation
Ian Delgadillo Bonequi, Abraham Stroschein, Lucas J. Koerner
Summary: This article describes a custom and open-source FPGA-based data acquisition system that is used for electrophysiology and closed-loop feedback experiments. The system combines FPGA acquisition and processing with high-speed analog and digital converters to enable real-time feedback. The digital approach simplifies experimental setup and repeatability by allowing for system identification and in situ tuning of filter bandwidths.
REVIEW OF SCIENTIFIC INSTRUMENTS
(2022)
Article
Biochemistry & Molecular Biology
Motoki Okui, Tatsuro Murakami, Hongxin Sun, Chiaki Ikeshita, Narisato Kanamura, Akiyuki Taruno
Summary: CALHM1 and 3 subunits are N-glycosylated at single Asn residues in their second extracellular loops, controlling the biosynthesis and gating kinetics of the CALHM1/3 channel. CALHM3 is reversibly palmitoylated at three intracellular Cys residues, with palmitoylation playing a critical role in CALHM1/3 activity.
Article
Neurosciences
Kevin Jehasse, Laurent Massotte, Sebastian Hartmann, Romain Vitello, Sofian Ringlet, Marie Vitello, Han Chow Chua, Stephan A. Pless, Dominique Engel, Jean-Francois Liegeois, Bernard Lakaye, Jochen Roeper, Vincent Seutin
Summary: The research showed that the compound XG selectively inhibits the pacemaking of dopamine neurons without affecting their bursting behavior. It demonstrated specificity in its effects on different types of neurons and seemed to target a small subthreshold XG-sensitive current carried by Na+ and Cl- ions. The molecular target of XG and its precise mechanism of action remain to be elucidated, but these findings represent a significant step towards understanding pacemaking in dopamine neurons.
Article
Nanoscience & Nanotechnology
Jiaru Fang, Xinwei Wei, Hongbo Li, Ning Hu, Xingxing Liu, Dongxin Xu, Tao Zhang, Hao Wan, Ping Wang, Xi Xie
Summary: Cardiovascular diseases pose a significant threat to human health, but drug development is time-consuming and costly. Researchers have developed a drug assessment platform based on an electrical-mechanical synchronized biosensing system, which allows for high-throughput and time-dependent assessment. The system can record and analyze dynamic responses of cardiomyocytes to drugs, enabling rapid and effective drug screening.
MICROSYSTEMS & NANOENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Liying Dong, Qiqi Zhou, Qianqian Liang, Zhen Qiao, Yani Liu, Liming Shao, Kewei Wang
Summary: In this study, a novel TRPV1 agonist CPIPC was identified and shown to have analgesic activity in mice.
Article
Medicine, Research & Experimental
J. V. Bukanova, E. I. Solntseva, R. V. Kondratenko, V. G. Skrebitsky
Summary: Ivermectin, a macrocyclic lactone used as an antiparasitic agent, is being researched for its potential in treating cancer and viral diseases. This study showed that neuronal glycine receptors are highly sensitive to Ivermectin.
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Article
Neurosciences
Yasuhiro Fukushima, Yutaka Yamaguti, Shigeru Kuroda, Takeshi Aihara, Ichiro Tsuda, Minoru Tsukada
Summary: The study revealed the presence of a Cantor coding-like iterated function system in the hippocampal CA1 network, which describes the system's properties based on spatial patterns and EPSP amplitudes. Experimental findings indicated specific physiological basis of the system regarding intervals of sequential input and dependency on inhibitory networks.
COGNITIVE NEURODYNAMICS
(2021)
Review
Pharmacology & Pharmacy
Damian C. Bell, Luigi Leanza, Saverio Gentile, Daniel R. Sauter
Summary: Ion channels play key roles in various physiological processes, and their dysfunction can lead to channelopathies, including cancer. The field of ion channel and cancer research has rapidly grown in the past two decades, allowing us to gain deeper insights into the mechanisms of ion channels in cancer and develop therapeutic interventions.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Neurosciences
Z. Chen, D. H. Terman, S. P. Travers, J. B. Travers
Summary: Responses in the gustatory nucleus of the solitary tract (rNST) are modulated by synaptic interactions and membrane properties. The potassium current I-A is a potential modulator. This study explored the role of I-A in modulating the response to afferent stimulation and GABA-mediated inhibition in the rNST using transgenic mice expressing channelrhodopsin in GABAergic neurons.
Article
Medicine, General & Internal
M. Paucar, J. Lundin, T. Alshammari, A. Bergendal, M. Lindefeldt, M. Alshammari, G. Solders, J. Di Re, I. Savitcheva, T. Granberg, F. Laezza, E. Iwarsson, P. Svenningsson
JOURNAL OF INTERNAL MEDICINE
(2020)
Article
Multidisciplinary Sciences
Giuseppe Aceto, Claudia Colussi, Lucia Leone, Salvatore Fusco, Marco Rinaudo, Federico Scala, Thomas A. Green, Fernanda Laezza, Marcello D'Ascenzo, Claudio Grassi
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biochemistry & Molecular Biology
Nolan M. Dvorak, Paul A. Wadsworth, Pingyuan Wang, Haiying Chen, Jia Zhou, Fernanda Laezza
Article
Pharmacology & Pharmacy
Cynthia M. Tapia, Oluwarotimi Folorunso, Aditya K. Singh, Kathleen McDonough, Fernanda Laezza
Article
Biochemistry & Molecular Biology
Paul A. Wadsworth, Aditya K. Singh, Nghi Nguyen, Nolan M. Dvorak, Cynthia M. Tapia, William K. Russell, Clifford Stephan, Fernanda Laezza
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2020)
Article
Chemistry, Medicinal
Pingyuan Wang, Paul A. Wadsworth, Nolan M. Dvorak, Aditya K. Singh, Haiying Chen, Zhiqing Liu, Richard Zhou, Luis Marcelo F. Holthauzen, Jia Zhou, Fernanda Laezza
JOURNAL OF MEDICINAL CHEMISTRY
(2020)
Article
Neurosciences
Laura Stertz, Jessica Di Re, Guangsheng Pei, Gabriel R. Fries, Emily Mendez, Shenglan Li, Laura Smith-Callahan, Henriette Raventos, Jerricho Tipo, Rohan Cherukuru, Zhongming Zhao, Ying Liu, Peilin Jia, Fernanda Laezza, Consuelo Walss-Bass
Summary: This study utilized human-induced pluripotent stem cells (hiPSCs) derived from schizophrenia (SCZ) patients in the isolated population of the Central Valley of Costa Rica for RNA-sequencing analysis, identifying differentially expressed genes enriched in the phosphoinositide 3-kinase/glycogen synthase kinase 3 (PI3K/GSK3) signaling pathway, and suggesting disruption of the PI3K/GSK3 signaling pathway as a potential risk factor for SCZ.
NEUROPSYCHOPHARMACOLOGY
(2021)
Article
Neurosciences
Elizabeth J. Crofton, Miroslav N. Nenov, Yafang Zhang, Cynthia M. Tapia, Joseph Donnelly, Shyny Koshy, Fernanda Laezza, Thomas A. Green
Summary: This study identified NAcSh-enhanced transcripts using a novel transcriptomic analysis method and demonstrated that manipulating the retinoic acid pathway in the NAcSh can decrease cocaine self-administration, providing a promising pathway for pharmacotherapeutic development against cocaine use disorder.
Article
Biochemistry & Molecular Biology
Mate Marosi, Miroslav N. Nenov, Jessica Di Re, Nolan M. Dvorak, Musaad Alshammari, Fernanda Laezza
Summary: In this study, it was found that Akt inhibition enhances peak transient Na+ current density through Na(v)1.6 channels and increases action potential firing in CA1 pyramidal neurons, accompanied by a decrease in action potential current threshold. Complementary confocal analysis revealed that Akt inhibition is associated with the lengthening of Na(v)1.6 fluorescent intensity along the axonal initial segment, providing a mechanism for augmented neuronal excitability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Nolan M. Dvorak, Cynthia M. Tapia, Timothy J. Baumgartner, Jully Singh, Fernanda Laezza, Aditya K. Singh
Summary: This study reveals the selective modulatory effects of Wee1 kinase inhibitor on Na(v)1.2 channel activity, emphasizing the importance of cellular signaling pathways in fine-tuning neuronal excitability.
Review
Biochemistry & Molecular Biology
Mate Marosi, Parsa Arman, Giuseppe Aceto, Marcello D'Ascenzo, Fernanda Laezza
Summary: GSK3 beta is a multifunctional kinase expressed in all eukaryotic cells, with a central role in intracellular signaling. It phosphorylates voltage-gated ion channels, affecting neuronal excitability, plasticity, and behavior. Imbalanced GSK3 beta activity may increase vulnerability of neuronal circuitry and the risk for neuropsychiatric disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biology
Timothy J. Baumgartner, Zahra Haghighijoo, Nana A. Goode, Nolan M. Dvorak, Parsa Arman, Fernanda Laezza
Summary: Alzheimer's disease is characterized by two major histopathological abnormalities: extracellular plaques composed of amyloid beta and intracellular hyperphosphorylated tau. Attenuation of hippocampal hyperactivity, which is linked to dysfunction of voltage-gated Na+ channels, has emerged as a promising therapeutic strategy for early-stage AD. This review explores the role of Nav channels in neuronal function, their connections to AD pathology, and their potential as therapeutic targets.
Article
Biochemistry & Molecular Biology
Nolan M. Dvorak, Cynthia M. Tapia, Aditya K. Singh, Timothy J. Baumgartner, Pingyuan Wang, Haiying Chen, Paul A. Wadsworth, Jia Zhou, Fernanda Laezza
Summary: Voltage-gated Na+ channels are crucial for action potentials, with isoforms like Na(v)1.1, Na(v)1.2, and Na(v)1.6 showing differential expression in the central nervous system. Targeting specific isoforms could lead to promising neuromodulators with potential therapeutic value.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Meeting Abstract
Biophysics
Nolan M. Dvorak, Paul Wadsworth, Pingyuan Wang, Haiying Chen, Jia Zhou, Fernanda Laezza
BIOPHYSICAL JOURNAL
(2021)
Meeting Abstract
Neurosciences
Paul A. Wadsworth, Aditya Singh, Nolan M. Dvorak, Nghi Nguyen, Giuseppe Aceto, Evan Troendle, Cynthia M. Tapia, Pingyuan Wang, Annie Wadsworth, Oluwarotimi Folorunso, Haiying Chen, Reid Powell, Martin Ulmschneider, Jia Zhou, Marcello D'Ascenzo, Clifford Stephan, Fernanda Laezza
NEUROPSYCHOPHARMACOLOGY
(2020)
Correction
Neurosciences
Lucia Privitera, Ellen L. Hogg, Matthias Gaestel, Mark J. Wall, Sonia A. L. Correa
Article
Neurosciences
Li-Ya Jiang, Guan-Hao Wang, Jing-Jiao Xu, Xiao-Li Li, Xiao-Yan Lin, Xiang Fang, Hong-Xu Zhang, Mei Feng, Chun-Ming Jiang
Summary: This study reveals the importance of LINC00473 in regulating temozolomide (TMZ) resistance in glioblastoma (GB) and its potential mechanism. By regulating the expression of CEBP alpha and MGMT, LINC00473 promotes the formation of chemoresistance. Furthermore, LINC00473 can transfer chemoresistance to adjacent sensitive cells through exosomes.
Article
Neurosciences
Olga Kopach, Tetyana Pivneva, Nataliya Fedirko, Nana Voitenko
Summary: This study found that diabetic animals exhibit severe xerostomia characterized by reduced saliva flow rate, diminished total protein content, and decreased amylase activity. The impaired saliva production in diabetes is associated with reduced and delayed intracellular Ca2+ signals in submandibular acinar cells, caused by malfunctioning mitochondria. Targeting malfunctioning mitochondria may be a potential strategy for the treatment of diabetic xerostomia.
Article
Neurosciences
Nicholas M. Timme, Cherish E. Ardinger, Seth D. C. Weir, Rachel Zelaya-Escobar, Rachel Kruger, Christopher C. Lapish
Summary: This study aimed to assess aversion-resistant drinking behavior in head-fixed mice and explore the relationship between non-consummatory behaviors and aversion-resistant drinking. The results showed that head-fixed mice exhibited heterogenous levels of aversion-resistant drinking and non-consummatory behaviors were related to the intensity of this behavior.
Article
Neurosciences
David R. Maguire, Charles P. France
Summary: Methocinnamox (MCAM) is a novel, long-acting opioid receptor antagonist that effectively decreases fentanyl self-administration and prevents opioid overdose in monkeys. The study demonstrates the potential therapeutic utility of MCAM in the treatment of opioid use disorder.
Article
Neurosciences
Xiang Li, Dan Feng, Shenglu Ma, Mingxing Li, Shulei Zhao, Man Tang
Summary: This study investigated the effects of fluoxetine on neurochemical, neurobiological, and neurobehavioral changes in different subregions of the hippocampus. The results showed that fluoxetine increased dialysate 5-HT, decreased membrane 5-HTT protein, and increased cytoplasmic fraction. Additionally, fluoxetine reduced immobility times in behavioral tests, with greater effects observed in the ventral subregion compared to the dorsal subregion.
Article
Neurosciences
Alexander V. Zholos, Mariia I. Melnyk, Dariia O. Dryn
Summary: Acetylcholine is an important neurotransmitter in visceral smooth muscles, activating M2 and M3 muscarinic receptors to cause smooth muscle excitation and contraction. This review focuses on the cellular and molecular mechanisms underlying acetylcholine-induced depolarisation and smooth muscle contraction, as well as the effects of anticholinergic drugs on gastrointestinal motility. The knowledge gained from recent studies has greatly expanded our understanding of these processes.
Article
Neurosciences
Zhenlong Li, Hsien-Yu Peng, Chau-Shoun Lee, Tzer-Bin Lin, Ming-Chun Hsieh, Cheng-Yuan Lai, Han-Fang Wu, Lih-Chyang Chen, Mei-Ci Chen, Dylan Chou
Summary: Methylone shows significant efficacy in treating depression and social deficits, making it an ideal candidate for anti-depressant medication.
Article
Neurosciences
Aline Freyssin, Allison Carles, Sarra Guehairia, Gilles Rubinstenn, Tangui Maurice
Summary: This study explores the potential of combining FENM and S1R agonists in the treatment of Alzheimer's disease. The results showed that most FENM-based combinations can protect against learning deficits caused by A beta 25-35, with better efficacy in short-term memory.
Article
Neurosciences
J. D. Lorente, J. Cuitavi, L. Rullo, S. Candeletti, P. Romualdi, L. Hipolito
Summary: This study analyzed the effects of pain on negative affect in different sexes and time courses, as well as the involvement of the dynorphinergic and corticotropin releasing factor systems in these pain-related behaviors. The results showed sex and time-dependent anxiety- and anhedonia-like behaviors induced by pain in female rats. The recruitment of KOR/DYN in the NAc was identified as a key neurological substrate mediating pain-induced behavioral alterations.
Article
Neurosciences
Rongjun Liu, Daofan Sun, Xiuzhong Xing, Qingge Chen, Bo Lu, Bo Meng, Hui Yuan, Lan Mo, Liufang Sheng, Jinwei Zheng, Qiusheng Wang, Junping Chen, Xiaowei Chen
Summary: The coexistence of pain and depression is frequently observed in patients with chronic pain and depression. Oxytocin, a neuropeptide, has been reported to relieve chronic pain and depressive symptoms. This study investigated the effect of intranasal oxytocin on neuropathic pain and comorbid depressive symptoms, and found that oxytocin attenuated depression-like behavior but did not alleviate mechanical hyperalgesia. The results suggest that intranasal oxytocin may have the potential to treat depressive symptoms in neuropathic pain patients.