Article
Biophysics
Sarah Kosta, Dylan Colli, Qiang Ye, Kenneth S. Campbell
Summary: FiberSim is a flexible open-source model that simulates myofilament-level contraction, allowing for the exploration of mechanisms modulated by myosin-binding protein C and varying isoform expression levels. This user-friendly software is designed for efficient simulations on regular laptops and may accelerate research focused on sarcomere contractile properties.
BIOPHYSICAL JOURNAL
(2022)
Article
Agriculture, Dairy & Animal Science
Eva Tvrda, Ondrej Bucko, Kristina Rojkova, Michal Duracka, Simona Kunova, Jan Kovac, Filip Benko, Miroslava Kacaniova
Summary: This study evaluated the effectiveness of selected semen extenders in preventing bacterial overgrowth in boar ejaculates stored for 72 hours. While extenders with antibiotics maintained sperm vitality, they did not completely eliminate bacteria from the extended semen. Some bacterial isolates showed resistance to certain antibiotics.
Article
Biochemistry & Molecular Biology
Meishan Li, Lucia Coppo, Bhanu P. Jena, Lars Larsson
Summary: This study optimized quantum dot mediated thermometry to measure the efficiency of myosin extracted from muscle mini bundles. The results showed that different myosin isoforms have different efficiencies, with lower efficiency observed in slow myosin isoforms. This study is crucial for the evaluation of neuromuscular disorders.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Tianbang Wang, Emrulla Spahiu, Jennifer Osten, Florentine Behrens, Fabius Grunhagen, Tim Scholz, Theresia Kraft, Arnab Nayak, Mamta Amrute-Nayak
Summary: This study investigated the mechanical and chemical properties of myosin at the single-molecule level in rabbit heart ventricle and soleus muscles. The myosins from these two sources, despite expressing the same MyHC isoform, displayed distinct motile and ATPase kinetic properties. The presence of a specific myosin light chain 1 isoform in soleus muscles may contribute to the observed differences in myosin kinetics.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yurii S. Borovikov, Daria D. Andreeva, Stanislava Avrova, Vladimir V. Sirenko, Armen O. Simonyan, Charles S. Redwood, Olga E. Karpicheva
Summary: Mutations in the muscle isoforms of tropomyosin genes can lead to muscle diseases. The R90P mutation in gamma-tropomyosin is associated with abnormal muscle Ca2+-sensitivity and muscle weakness. This mutation inhibits the interaction between tropomyosin and actin, affecting the functionality of the troponin-tropomyosin complex and the balance of myosin heads in the ATPase cycle, potentially causing the observed muscle dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biophysics
Larry S. Tobacman
Summary: Recent advances in understanding the structural basis of troponin-tropomyosin's regulation of striated muscle contraction, particularly through cryo-electron microscopy data, have revealed compelling atomic models for both apo- and Ca2+-saturated states. Subsequent analyses have further elucidated these findings, showing the hindering effects of troponin and tropomyosin on myosin-actin binding in relaxed muscle and the specific bindings in the Ca2+-saturated state.
BIOPHYSICAL JOURNAL
(2021)
Article
Cell Biology
Masafumi Tanji, Keitaro Wada, Keita Sakamoto, Yudai Ono, Masafumi Inui
Summary: Myosin heavy chains (MyHCs) are the most abundant proteins in myofiber and are encoded by myosin heavy chain (Myh) genes. The expression patterns of Myh genes/MyHC proteins are considered as molecular signatures of specific fiber types or the regenerative status of mammalian skeletal muscles. Immunohistochemistry is costly and not ideal for whole-mount samples, while in situ hybridization (ISH) is not commonly applied for Myh genes due to their highly homologous sequences. However, a new method of ISH analysis using the untranslated region (UTR) sequence of Myh genes has been shown to be cost-effective and specific for analyzing Myh gene expression in whole-mount samples.
DEVELOPMENT GROWTH & DIFFERENTIATION
(2023)
Article
Multidisciplinary Sciences
Ananya Tripathi, Charles Bond, James R. Sellers, Neil Billington, Yasuharu Takagi
Summary: Myosin proteins bind and interact with filamentous actin and perform specific functions in cells. The mechanobiology of myosins can be studied through in vitro motility assays, while fluorescence microscopy-based assays can be used to determine parameters such as actin translocation velocities. These techniques are valuable tools for investigating the dynamics of myosins and their role in cellular processes.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Biochemistry & Molecular Biology
Li Zhu, Maicon Landim-Vieira, Michelle Rodriguez Garcia, Jose R. Pinto, Joseph M. Chalovich
Summary: The basic C-terminal region of human troponinT (TnT) plays a crucial role in regulating actomyosin ATPase activity in response to calcium levels. Phosphomimetic-like mutants of TnT were generated to identify key basic residues in this region. The S275D and T277D mutants showed the highest level of ATPase activation, while the T284D mutant had the smallest effect. These findings suggest that negative charge placement in the C-terminal region of TnT, particularly near the IT helix and adjacent to basic residues, significantly impacts its regulatory function.
Article
Agriculture, Multidisciplinary
Fang Lin, Winnie C. Soko, Jing Xie, Hongyan Bi
Summary: This study aims to evaluate fish muscle exudate proteins to discover potential allergens for allergy tests. Magnetic beads modified with an IgE antibody were used to isolate allergens in the exudate sample. Eight allergens from large yellow croaker exudate were successfully identified. The proposed microfluidic-magnetic-based allergen extraction protocol provides a facile and rapid test for seafood allergies, addressing food safety issues for allergic populations.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Li Zhu, Dylan Johnson, Joseph M. Chalovich
Summary: The C-terminal 14-16 residues of human troponin T are crucial for full inactivation and prevent full activation at saturating Ca2+. The basic residues within this region are essential for the function of TnT, but the mechanism of action is still unknown.
Review
Biology
Suman Nag, Darshan Trivedi
Summary: The discovery of two functional states of myosin in muscle contraction has shed light on their role in adaptive thermogenesis and increasing cardiac muscle power. This equilibrium state is shown to be affected by various physiological and pathophysiological perturbations.
Review
Biochemistry & Molecular Biology
O. E. Karpicheva
Summary: Congenital myopathies are a group of clinically and genetically heterogeneous diseases with a wide range of variants, making diagnosis complicated and treatment limited to symptomatic care. Research on molecular mechanisms involving mutant muscle proteins offers potential for new perspectives on differential diagnosis and therapy.
JOURNAL OF EVOLUTIONARY BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Review
Physiology
Matthias Lambert, Charlotte Claeyssen, Bruno Bastide, Caroline Cieniewski-Bernard
Article
Biochemical Research Methods
Julie Fourneau, Caroline Cieniewski-Bernard, Marie-Helene Canu, Sophie Duban-Deweer, Johann Hachani, Bruno Bastide, Erwan Dupont
JOURNAL OF NEUROSCIENCE METHODS
(2020)
Article
Biochemistry & Molecular Biology
Julie Fourneau, Marie-Helene Canu, Erwan Dupont
Summary: This study investigated the synaptic plasticity process in the somatosensory primary cortex during a period of sensorimotor perturbation and recovery. The results showed that changes in the presynaptic compartment occurred as early as D7 during SMP, followed by changes in the postsynaptic compartment at D14, and these changes persisted at least until 24 hours of recovery. Proteins related to synapse structure were mildly affected, primarily at D14.
JOURNAL OF MOLECULAR NEUROSCIENCE
(2021)
Article
Cell Biology
Samir Bensaid, Claudine Fabre, Mehdi Pawlak-Chaouch, Caroline Cieniewski-Bernard
Summary: Electrical stimulation and oxygenation therapy are two effective treatments against hypoxia-induced muscle atrophy, acting through different molecular adaptations.
CELL AND TISSUE RESEARCH
(2022)
Article
Neurosciences
Marie-Helene Canu, Valerie Montel, Julie Dereumetz, Tanguy Marqueste, Tanguy Decherchi, Jacques-Olivier Coq, Erwan Dupont, Bruno Bastide
Summary: Research indicates that early movement restriction can lead to severe atrophy and functional disorganization in the soleus muscle of rats, resulting in issues such as sensorimotor restriction and increased neuromuscular excitability. However, some of these changes can be reversed when typical activity is resumed, suggesting the importance of proprioceptive feedback in promoting typical motor function development.
EXPERIMENTAL NEUROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Theo Fovet, Corentin Guilhot, Laurence Stevens, Valerie Montel, Pierre Delobel, Remi Roumanille, Michel-Yves Sempore, Damien Freyssenet, Guillaume Py, Thomas Brioche, Angele Chopard
Summary: Muscle deconditioning, resulting from muscle inactivity, causes rapid decrease in muscle strength and mass, necessitating research on early changes and effective prevention methods. Findings from a dry immersion experiment suggest that the major effects of muscle deconditioning occur during the initial days of inactivity, indicating the importance of targeting this time period for countermeasures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Charlotte Claeyssen, Bruno Bastide, Caroline Cieniewski-Bernard
Summary: Desmin plays a crucial role in maintaining muscle integrity and cell homeostasis. Post-translational modifications and O-GlcNAcylation regulate Desmin, and their interplay affects Desmin's behavior, phosphorylation level, and partition towards the cytoskeleton.
SCIENTIFIC REPORTS
(2022)
Article
Medicine, Research & Experimental
Alexis Boulinguiez, Christian Duhem, Alicia Mayeuf-Louchart, Benoit Pourcet, Yasmine Sebti, Kateryna Kondratska, Valerie Montel, Stephane Delhaye, Quentin Thorel, Justine Beauchamp, Aurore Hebras, Marion Gimenez, Marie Couvelaere, Mathilde Zecchin, Lise Ferri, Natalia Prevarskaya, Anne Forand, Christel Gentil, Jessica Ohana, France Pietri-Rouxel, Bruno Bastide, Bart Staels, Helene Duez, Steve Lancel
Summary: The study shows that NR1D1 plays a crucial role in regulating skeletal muscle SR calcium homeostasis by repressing the expression of the SERCA inhibitor myoregulin. Lower NR1D1 expression is observed in patients with Duchenne muscular dystrophy, while pharmacological activation of NR1D1 improves calcium homeostasis and muscle structure and function.
Article
Biology
Alexandre Pierre, Claire Bourel, Raphael Favory, Benoit Brassart, Frederic Wallet, Frederic N. N. Daussin, Sylvain Normandin, Michael Howsam, Raphael Romien, Jeremy Lemaire, Gaelle Grolaux, Arthur Durand, Marie Frimat, Bruno Bastide, Philippe Amouyel, Eric Boulanger, Sebastien Preau, Steve Lancel
Summary: Sepsis is a life-threatening infection that leads to muscle weakness, which impairs short- and long-term prognoses. This study investigated the impact of an energy deficit on skeletal muscle during the early stages of sepsis. The results showed that a sepsis-like energy deficit did not explain the muscle fiber atrophy and mitochondrial dysfunction observed in sepsis, but led to specific metabolic adaptations not found in sepsis.