Article
Biochemistry & Molecular Biology
Yasuo Ito, Harumitsu Nagoya, Masamizu Yamazato, Yoshio Asano, Masahiko Sawada, Tomokazu Shimazu, Makiko Hirayama, Toshimasa Yamamoto, Nobuo Araki
Summary: This study investigated the effects of aging on nitric oxide (NO) production during cerebral ischemia-reperfusion (IR). The results showed that the change in NO3- levels in the plasma of older rats was lower compared to younger rats during cerebral ischemia-reperfusion. Hypertension and aging may be important factors impacting NO production in brain IR injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Takaaki Aratake, Youichirou Higashi, Tomoya Hamada, Yusuke Ueba, Takahiro Shimizu, Shogo Shimizu, Toshio Yawata, Tetsuya Ueba, Motoaki Saito
Summary: Accumulating evidence suggests that there are time-of-day variations in ischemic neuronal injury, with zinc ion accumulation displaying circadian fluctuations through diurnal variations in EAAC1 expressions, affecting susceptibility to ischemic neuronal injury in the hippocampal hilar region.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Chemistry, Analytical
Matthew Humbert, Saiid Lewis, Anthony Borgerding
Summary: The method using a gas-phase microdialysis probe interfaced with a modified commercially available nitric oxide detector can selectively measure aqueous NO at low levels with high selectivity. The quick response time, continuous extraction, and rugged nature of the method make it useful for monitoring NO in biological applications.
Article
Soil Science
Eric W. Slessarev, Aral C. Greene, Peter M. Homyak, Samantha C. Ying, Joshua P. Schimel
Summary: Wetting dry soil leads to emission of nitric oxide (NO), originating from non-biological and biologically-mediated processes. Continuous observations identified two distinct phases of NO production: an initial phase and a prolonged phase, with the former influenced by soil acidity and exchangeable calcium, and the latter associated with nitrite accumulation. These findings provide insights for improving mechanistic models of NO emissions from soils and enhancing predictions of the terrestrial N cycle and atmospheric chemistry.
SOIL BIOLOGY & BIOCHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Slobodan Tepic, Daniel Arens, Tim Buchholz, Dirk Nehrbass, Olivera Cvetkovic, Martin J. Stoddart, R. G. Richards, Stephan Zeiter
Summary: Pneumonia, especially during the COVID-19 pandemic, has become a major public health and economic disaster. This study suggests that lung metabolism in inflammation may have common features regardless of the underlying cause. By comparing mice with bleomycin-induced lung inflammation to control groups, it was found that certain amino acids had significantly lower concentrations in the arterial blood, indicating that arginine replacement therapy may be beneficial in pneumonia.
Article
Chemistry, Multidisciplinary
Wen Zhou, Wanxiang Yang, Keyu Fan, Wuyang Hua, Shaohua Gou
Summary: In this study, a novel hypoxia-activated NO donor (Hano) was designed and prepared, which can increase NO release, dilate blood vessels, and attenuate myocardial hypoxia injury. This drug shows great potential for the treatment of hypoxic heart diseases.
Article
Cardiac & Cardiovascular Systems
Jody L. Greaney, Erika F. H. Saunders, Lacy M. Alexander
Summary: Activation of NF-kappa B, through increased vascular ROS production, contributes to reduced NO-dependent endothelial dilation in young adults with MDD. Short-term treatment with NF-kappa B inhibitor salsalate improves NO-mediated endothelium-dependent dilation in MDD patients, while acute localized ROS scavenging with apocynin enhances NO-dependent dilation before salsalate administration.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
James T. Miller, Casey G. Turner, Jeffrey S. Otis, Yesser Sebeh, Matthew J. Hayat, Arshed A. Quyyumi, Brett J. Wong
Summary: Our study found that inducible nitric oxide synthase (iNOS) plays a role in the reduced nitric oxide (NO)-dependent vasodilation in prehypertensive individuals and Black participants. Inhibition of iNOS can increase endothelial NO-dependent vasodilation in prehypertensive White participants and in both normotensive and prehypertensive Black participants.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Physiology
Casey G. Turner, Anna E. Stanhewicz, Karen E. Nielsen, Jeffrey S. Otis, Rafaela G. Feresin, Brett J. Wong
Summary: The purpose of this study was to compare endothelial function and NO-dependent vasodilation between women in different hormonal phases and men. The results showed that men had greater endothelium-dependent vasodilation, but lower NO-dependent vasodilation compared to women. Subgroup analysis revealed that women using oral contraceptive pills had significantly greater NO-dependent vasodilation. This study highlights the importance of quantifying NO-dependent vasodilation and provides important implications for experimental design and data interpretation.
JOURNAL OF APPLIED PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Kristie Huda, Dylan J. Lawrence, Weylan Thompson, Sarah H. Lindsey, Carolyn L. Bayer
Summary: The authors demonstrate the potential of photoacoustic tomography to capture significant acute vasodilation of major arteries and vascular selectivity, providing valuable preclinical information on vascular response that cannot be obtained by other methods.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Giuditta Gambino, Daniele Gallo, Ana Covelo, Giuseppe Ferraro, Pierangelo Sardo, Giuseppe Giglia
Summary: This study investigated the involvement of TRPV1 in nitric oxide-mediated influence on hippocampal processes. The findings suggest that TRPV1 channels can be activated to modulate excitatory neurotransmission in the hippocampus, and this effect is linked to nitric oxide production.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Stephanie M. Mutchler, Mahpara Hasan, Donald E. Kohan, Thomas R. Kleyman, Roderick J. Tan
Summary: Our study demonstrates that deletion of the gamma subunit of ENaC, while allowing for greater activation of eNOS, is not sufficient to prevent IRI. This suggests that the protective effects of alpha subunit deletion may be due, in part, to other mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Medicinal
Mingju Wen, Jianbo Sun, Miao Yang, Xueling Zhang, Yue Wang, Wen Zhou, Yuning Shi, Yujing Huang, Na Li, Li Chen
Summary: Twelve new hybrid compounds of Esculetin with nitric oxide (NO) donors and/or mitochondrial targeting groups were designed, synthesized, and evaluated for their anti-tumor activity and mechanism in vitro and in vivo. The most potent compound A11 exhibited nanomolar antiproliferative activity on triple-negative breast cancer (TNBC) MDA-MB-231 cells, with a selective inhibitory effect. The mechanism involved mitochondrial targeting, releasing a high concentration of NO, increasing the expression of CypD, and triggering cancer cell apoptosis through increased ROS production, along with cell cycle arrest at the G2/M phase. Moreover, A11 demonstrated superior TNBC inhibition rate and reduced toxicity compared to doxorubicin (DOX) in vivo.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Fang Yuan, Cuiling Zhang, Xianzhu Luo, Xiaokun Shen, Yuezhong Xian
Summary: ATP stimulus-responsive tetrahedral DNA-gated fluorescent covalent organic frameworks (COFs) were developed for estradiol (E2) delivery and controllable release. The fluorescent COFs with an efficient E2 loading showed great potential against myocardial ischemia and reperfusion injury.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Francisco Estrada-Rojo, Liliana Carmona-Aparicio, Virginia Arriaga-Avila, Elvia Coballase-Urrutia, Adan Perez-Arredondo, Rosalinda Guevara-Guzman, Noemi Cardenas-Rodriguez, Luz Navarro
Summary: Amino acid neurotransmitters play crucial roles in physiological and pathological processes, with their concentrations affected by external factors such as the light-dark cycle. Research has revealed significant variations in the concentrations of amino acid neurotransmitters and the GLX index over a 24-hour period.