期刊
NEUROIMAGE
卷 60, 期 2, 页码 933-939出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.neuroimage.2012.01.100
关键词
Mouse brain; Tissue fixation; Perfusion; Paraformaldehyde; MRI
资金
- Canada Foundation for Innovation
- Ontario Innovation Trust
- Canadian Institutes of Health Research, Genome Canada
- National Cancer Institute of Canada
- National Institutes of Health, Ontario Institute for Cancer Research
- Ontario Research and Development Challenge Fund
In fixed mouse brain magnetic resonance images, a high prevalence of fixation artifacts have been observed. Of more than 1700 images of fixed brains acquired at our laboratory, fixation artifacts were present in approximately 30%. In this study, two of these artifacts are described and their causes are identified. A hyperintense rim around the brain is observed when using perfusates reconstituted from powder and delivered at a high flow rate. It is proposed that these perfusion conditions cause blockage of the capillary beds and an increase in pressure that ruptures the vessels, resulting in a blister of liquid below the dura mater. Secondly, gray-white matter contrast inversion is observed when too short a fixation time or too low a concentration of fixative is used, resulting in inadequate fixation. The deleterious consequences of these artifacts for quantitative data analysis are discussed, and precautions for their prevention are provided. (c) 2012 Elsevier Inc. All rights reserved.
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