期刊
NEUROGASTROENTEROLOGY AND MOTILITY
卷 24, 期 2, 页码 113-E82出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2982.2011.01841.x
关键词
inflammation; irritable bowel syndrome; mast cells; protease activated receptors
资金
- National Natural Science Foundation of China [30700358]
- Outstanding Youth scientist research Foundation of Shandong Province [2007BS03044]
Background Growing evidence suggests that protease activated receptors (PARs) are mediators of persistent neuropathic pain, but their possible function as mediators in patients with post infectious irritable bowel syndrome (PI-IBS) remains to be further explored. This article aims to investigate the expression of PAR2 and PAR4 in the colonic mucosa of patients with PI-IBS, focusing on correlation with mast cell activation status. Methods A total of 17 normal controls and 23 patients with PI-IBS volunteered the study. The expression and localization of PAR(2) and PAR(4) were investigated by RT-PCR and immunohistochemistry, and the expression of PAR2 and PAR4 in the mast cells was examined using double-immunofluorescence staining. Key Results The immunohistochemical study revealed that epithelial and submucosal cells showed immunoreactivity for both PAR2 and PAR4. Protease activated receptor 4 mRNA expression and immunoreactivity were down-regulated in PI-IBS compared with the control group. Specifically, a reduced immunoreactivity for PAR4 was observed in mast cells of PI-IBS compared with normal controls, whereas there are no significant differences shown in PAR2 between the PI-IBS and the control group. It is also found that the PAR4 immunoreactivity decreases, while the activity of mast cells increases in PI-IBS rather than normal controls. Conclusions & Inferences This study outlines the down-regulation of PAR4 in the mast cells of PI-IBS. It could be of considerable interests in understanding the mechanisms involved in the persistent colonic hypersensitivity and their potential role as therapeutic targets for PI-IBS.
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