Article
Multidisciplinary Sciences
Nardy Lampl, Raz Lev, Idan Nissan, Gal Gilad, Matanel Hipsch, Shilo Rosenwasser
Summary: The transmission of reductive and oxidative signals in the photosynthetic electron transport chain is crucial for coordinating photosynthetic activities. This study introduced a peroxiredoxin-based biosensor to monitor the dynamic changes in photosynthetically derived oxidative signaling. The results showed that the oxidation states of the biosensor reflected similar patterns to those of endogenous peroxiredoxin under different light conditions. The study also demonstrated the induction of oxidative signals and their inverse relationship with NADPH levels, suggesting the involvement of both reducing and oxidizing signals in photosynthesis regulation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Latika Nagpal, Michael D. Kornberg, Lauren K. Albacarys, Solomon H. Snyder
Summary: The study reveals the significant role of inositol hexakisphosphate kinase 2 (IP6K2) in brain function, demonstrating its interaction with creatine kinase-B (CK-B) to regulate cellular energy homeostasis and neuroprotection.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Enrica Federti, Alessandro Matte, Veronica Riccardi, Kevin Peikert, Seth L. Alper, Adrian Danek, Ruth H. Walker, Angela Siciliano, Iana Iatcenko, Andreas Hermann, Lucia De Franceschi
Summary: Peroxiredoxins (PRXs) play a crucial role in neurodegenerative disorders, and their activation can be restored by LYN inhibition in a mouse model of Chorea-acanthocytosis/Vps13A disease. Protein oxidation, NRF2 activation, and upregulation of cytoprotective systems were observed in the basal ganglia, along with increased expression of PRX2/5 in Vps13a(-/-) mice. LYN deficiency resulted in reduced NRF2 activation and down-regulated expression of PRX2/5, SRXN1, and TRXR. Nilotinib treatment reduced oxidative stress and plasma PRX5 levels in Vps13a(-/-) mice, suggesting plasma PRX5 as a potential biomarker for Chorea-acanthocytosis/Vps13A disease.
Article
Orthopedics
Hirohisa Uehara, Yoshiaki Itoigawa, Tomoki Wada, Daichi Morikawa, Akihisa Koga, Hidetoshi Nojiri, Takayuki Kawasaki, Yuichiro Maruyama, Muneaki Ishijima
Summary: This study investigated the relationship of oxidative stress and superoxide dismutase with rotator cuff degeneration using rat models. Results showed an increase in degeneration scores and oxidative stress in both injury and tear models. Superoxide dismutase may be associated with degenerative rotator cuff without tear but not after tear.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Article
Cardiac & Cardiovascular Systems
Jiayi Liu, Wei Li, Ke-Qiong Deng, Song Tian, Hui Liu, Hongjie Shi, Qian Fang, Zhen Liu, Ze Chen, Tian Tian, Shanyu Gan, Fengjiao Hu, Manli Hu, Xu Cheng, Yan-Xiao Ji, Peng Zhang, Zhi-Gang She, Xiao-Jing Zhang, Shaoze Chen, Jingjing Cai, Hongliang Li
Summary: The study found a close correlation between Trim16 expression and hypertrophy-related heart failure in both humans and mice. Trim16 deficiency exacerbated cardiomyocyte enlargement, while Trim16 overexpression attenuated cardiac hypertrophy and remodeling. Mechanistically, Prdx1 is an essential target of Trim16 in cardiac hypertrophy, and the Trim16-Prdx1 axis represents a promising therapeutic strategy for hypertrophy-related heart failure.
CIRCULATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Shin Koike, Kazuya Toriumi, Sakura Kasahara, Yosuke Kibune, Yo-ichi Ishida, Takashi Dan, Toshio Miyata, Makoto Arai, Yuki Ogasawara
Summary: This study used Glo1-knockout and vitamin B6-deficient mice as a CS-SCZ model, finding significantly increased carbonyl protein levels, particularly MG-H1-modified proteins, in various regions of the brain. Further studies focusing on MG-H1-modified and accumulated proteins in the hippocampus may reveal the onset mechanism of CS-SCZ induced by MGO-induced oxidative damages.
Article
Agriculture, Dairy & Animal Science
Walid S. Habashy, Marie C. Milfort, Romdhane Rekaya, Samuel E. Aggrey
Summary: Performance traits and mortality of chickens are negatively impacted by heat stress, with the thioredoxin pathway system potentially offering clues for nutritional strategies to mitigate these effects.
Review
Biology
Pratik Thapa, Hong Jiang, Na Ding, Yanning Hao, Aziza Alshahrani, Qiou Wei
Summary: Peroxiredoxins (Prxs) are widely expressed antioxidant enzymes in human tissues, and they are also present in archaea, bacteria, and eukaryota. Prxs play a crucial role in defending against oxidative stress due to their abundant expression in different cellular organelles and high sensitivity to H2O2. They are upregulated in cancer cells and can function as tumor promoters in various cancers. This review aims to summarize recent discoveries regarding the roles of Prxs in common cancer types and highlight their potential significance in improving cancer therapeutics and patient survival.
Article
Biochemistry & Molecular Biology
Yunfeng Yan, Xiaofei Huang, Yao Zhou, Jingyi Li, Feiyun Liu, Xueying Li, Xiaotao Hu, Jing Wang, Lingyan Guo, Renning Liu, Naoki Takaya, Shengmin Zhou
Summary: In Aspergillus nidulans, the peroxiredoxin (PrxA) plays a crucial role in protecting dormant conidia against high levels of H2O2, while catalase (CatB) becomes the main antioxidant during the mature hyphae stage. CatB needs to be secreted to function, and this process is limited to the mature hyphae stage.
Article
Biochemistry & Molecular Biology
Francesca Arfuso, Maria Rizzo, Claudia Giannetto, Elisabetta Giudice, Roberta Cirincione, Giovanni Cassata, Luca Cicero, Giuseppe Piccione
Summary: This study aimed to evaluate the changes in serum oxidant and antioxidant parameters, as well as the levels of homocysteine and muscle enzymes, in regularly trained athletic horses during official races of different distances. The results showed that exercise had an effect on oxidative stress markers, homocysteine, and muscle enzymes, while the race distance did not have a significant impact on these parameters.
Article
Agriculture, Dairy & Animal Science
M. Majdeddin, U. Braun, A. Lemme, A. Golian, H. Kermanshahi, S. De Smet, J. Michiels
Summary: Dietary guanidinoacetic acid (GAA) affects creatine (Cr) metabolic pathways, leading to increased cellular Cr and improved broiler performance. However, its impact on oxidative status remains unclear. This study showed that GAA can enhance muscle energy metabolism and indirectly support tolerance against oxidative stress.
Article
Biochemistry & Molecular Biology
Wangjuan Dai, Xiaofei Zou, Hongyan Jia, Yawen Peng, Boya La, Zhenjie Yan, Li Gao, Lianju Qin, Feiyang Diao, Xiang Ma, Yan Meng, Yugui Cui, Jiayin Liu
Summary: This study found that Prdx4 released by cumulus cells can repair meiotic defects in mouse oocytes and improve oocyte quality. Co-culturing immature oocytes with cumulus cells decreased oxidative stress and ER stress, leading to enhanced oocyte maturation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
W. M. Peeters, M. Gram, G. J. Dias, M. C. M. Vissers, M. B. Hampton, N. Dickerhof, A. E. Bekhit, M. J. Black, J. Oxboll, S. Bayer, M. Dickens, K. Vitzel, P. W. Sheard, K. M. Danielson, L. D. Hodges, J. C. Brond, J. Bond, B. G. Perry, L. Stoner, J. Cornwall, D. S. Rowlands
Summary: This study found that dietary supplementation with a novel keratin-derived protein may increase glucose clearance and modify tissue redox and insulin sensitivity in patients with type-2 diabetes.
Article
Biochemistry & Molecular Biology
Rocio Garcia-Arroyo, Elena B. Domenech, Carlos Herrera-Ubeda, Miguel A. Asensi, Cristina Nunez de Arenas, Jose M. Cuezva, Jordi Garcia-Fernandez, Federico Pallardo, Serena Mirra, Gemma Marfany
Summary: The retina is highly susceptible to genetic and environmental changes that result in oxidative stress and cell damage. CERKL is an important gene that protects against oxidative stress in the retina. This study using a mouse model found that Cerkl knockdown leads to abnormal stress response and cell death mechanisms in the retina, ultimately causing blindness.
Article
Biochemistry & Molecular Biology
Mars G. Sharapov, Sergey Gudkov
Summary: The study aimed to investigate the radioprotective properties of exogenous Prx1 and found that recombinant Prx1 is an effective radioprotector which reduces the severity of radiation-induced leuko- and thrombocytopenia, and protects bone marrow cells from damage.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Pharmacology & Pharmacy
Rachel Yoon Kyung Chang, Yuko Okamoto, Sandra Morales, Elizabeth Kutter, Hak-Kim Chan
Summary: This study aimed to formulate biologically stable phage hydrogels for controlled release of infective phages. The release profiles of phages were evaluated in different hydrogel formulations, showing promising results with certain combinations. The study demonstrated that certain hydrogels can maintain the stability of phages during storage.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
Waiting Tai, Michael Yee Tak Chow, Rachel Yoon Kyung Chang, Patricia Tang, Igor Gonda, Robert B. MacArthur, Hak-Kim Chan, Philip Chi Lip Kwok
Summary: Oral administration of hydroxychloroquine to COVID-19 patients is ineffective, but delivering it by inhalation may be a promising alternative. The isotonic, pH-neutral HCQS solutions exhibit good stability and are suitable for preclinical and clinical studies for potential COVID-19 treatment.
Review
Pharmacology & Pharmacy
Michael Yee Tak Chow, Waiting Tai, Rachel Yoon Kyung Chang, Hak -Kim Chan, Philip Chi Lip Kwok
Summary: In vitro - in vivo correlation refers to the predictive relationship between in vitro and in vivo data, particularly in the context of orally inhaled pharmaceutical aerosols. Correlating impactor data to lung deposition is a recent research area that can potentially optimize drug development efficiency.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Review
Pharmacology & Pharmacy
Yuncheng Wang, Rachel Yoon Kyung Chang, Warwick J. Britton, Hak-Kim Chan
Summary: Antimicrobial peptides and proteins play an increasingly important role in treating multidrug-resistant bacterial infections. Inhalation delivery of these agents can effectively increase local drug concentration in the lungs and reduce systemic toxicity. Targeted formulation strategies are needed to improve stability and inhalability of different antimicrobial peptides and proteins, as well as their combination with other antimicrobial agents. However, research on inhalable formulations of these agents is still underexplored, which is a crucial area for clinical translation.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Infectious Diseases
Rachel Yoon Kyung Chang, Michael Y. T. Chow, Yuncheng Wang, Chengxi Liu, Qixuan Hong, Sandra Morales, Andrew J. McLachlan, Elizabeth Kutter, Jian Li, Hak-Kim Chan
Summary: This study investigated the dose-response effect of Pseudomonas-targeting phage PEV31 in a mouse lung infection model. The results showed that phage treatment significantly reduced pulmonary bacterial load, regardless of the delivered phage dose. The study provides important insights for the dose selection of inhaled phage therapy for P. aeruginosa respiratory infections in humans.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Review
Respiratory System
Hak-Kim Chan, Rachel Yoon Kyung Chang
Summary: Studies have shown that inhaled bacteriophage therapy, particularly with PEV phages and powder formulations, has the potential to effectively combat respiratory infections caused by multidrug-resistant bacteria.
JOURNAL OF AEROSOL MEDICINE AND PULMONARY DRUG DELIVERY
(2022)
Article
Pharmacology & Pharmacy
Rachel Yoon Kyung Chang, Mengyu Li, Michael Y. T. Chow, Wei-Ren Ke, Waiting Tai, Hak-Kim Chan
Summary: Co-spray dried formulations of ciprofloxacin and hydrophobic D-amino acids offer dual benefits of anti-biofilm effect and moisture protection.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Ahmed H. Albariqi, Yuncheng Wang, Rachel Yoon Kyung Chang, Diana H. Quan, Xiaonan Wang, Stefanie Kalfas, John Drago, Warwick J. Britton, Hak-Kim Chan
Summary: This study investigated the pharmacokinetics and safety of inhaled dry powders of ivermectin and found that inhalation delivery improved the pharmacokinetic performance and maintained a high drug concentration in the respiratory tract, while the local toxicity was mild.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Review
Infectious Diseases
Chengxi Liu, Qixuan Hong, Rachel Yoon Kyung Chang, Philip Chi Lip Kwok, Hak-Kim Chan
Summary: This review paper summarizes the progress of phage-antibiotic therapy, including the mechanisms of Phage-Antibiotic Synergy (PAS) and its therapeutic performance. The choice of phages and antibiotics, administration time and sequence, and concentrations of the two agents have different impacts on bacterial inhibitory effects.
Review
Pharmacology & Pharmacy
Rachel Yoon Kyung Chang, Sue C. Nang, Hak-Kim Chan, Jian Li
Summary: The rise of antibiotic resistance has made antibiotic therapy ineffective against bacterial infections. ESKAPE pathogens, which exhibit high levels of resistance, pose a major global health threat. Novel antimicrobial therapeutic strategies, including bacteriophages, enzymes, immunomodulators, and monoclonal antibodies, are urgently needed to address this issue.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Pharmacology & Pharmacy
Rachel Yoon Kyung Chang, Yuko Okamoto, Sandra Morales, Elizabeth Kutter, Hak -Kim Chan
Summary: This study formulated liquid phage for topical delivery using a metered-dose spray and evaluated its properties and efficacy. The results showed that the optimized phage-polymer spray formulations were easily sprayable, with minimal dripping and fast drying time. The phages released rapidly from the formulation and effectively inhibited the growth of Pseudomonas aeruginosa. Both phages remained stable during storage for eight weeks. This study suggests that the spray formulations containing non-ionic polymers in ethanol/water could be a promising therapeutic system for delivering phages.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Review
Chemistry, Multidisciplinary
Rachel Yoon Kyung Chang, Hak-Kim Chan
Summary: Dry powder inhalation formulations have advantages over liquid formulations in drug delivery, but developing stable and inhalable formulations poses challenges. This review covers how to enhance the dispersibility of powder formulations through engineering methods and strategies to overcome challenges in particle engineering for biotherapeutics.
PHARMACEUTICAL RESEARCH
(2022)
Review
Pharmacology & Pharmacy
Wei-Ren Ke, Rachel Yoon Kyung Chang, Hak-Kim Chan
Summary: Dry powder inhalers (DPIs) can be used with a wide range of drugs and offer advantages for inhaled therapy. However, the conventional approach of using lactose blends as carrier particles may not meet certain demands. Innovative techniques and particle engineering strategies are needed to enhance drug delivery.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Review
Infectious Diseases
Sue C. Nang, Yu-Wei Lin, Aleksandra Petrovic Fabijan, Rachel Y. K. Chang, Gauri G. Rao, Jonathan Iredell, Hak-Kim Chan, Jian Li
Summary: This review examines the current literature on phage pharmacology and highlights major pharmacological challenges for phage therapy. The lack of PK/PD information represents a major challenge for phage therapy, as therapeutic phages are biological entities with complex PK/PD.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Pharmacology & Pharmacy
Yuncheng Wang, Dipesh Khanal, Adit B. Alreja, Hang Yang, Rachel Y. K. Chang, Waiting Tai, Mengyu Li, Daniel C. Nelson, Warwick J. Britton, Hak-Kim Chan
Summary: This study investigated the feasibility of producing inhalable powders of endolysins Cpl-1 and ClyJ-3 using spray drying method with excipients. The results showed that ClyJ-3 lost its bioactivity before the drying process, while Cpl-1 maintained its bioactivity in the spray-dried powders. Cpl-1 formulations with leucine and either lactose or trehalose exhibited promising physico-chemical properties and aerosol performances. These findings suggest that endolysin Cpl-1 can be formulated as spray dry powders for potential treatment of pulmonary infections.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Hui-Ting Huang, Shun-Fen Tzeng
Summary: Our study demonstrates the role of interleukin-33 (IL-33) in a demyelinating mouse model induced by cuprizone (CPZ), showing that IL-33 can alleviate the reduction of APC+ OLs and the decline of IL-33 levels in the corpus callosum, and promote the expression of myelin basic protein (MBP).
NEUROCHEMISTRY INTERNATIONAL
(2024)