Review
Biochemistry & Molecular Biology
Massimo Ubaldi, Nazzareno Cannella, Anna Maria Borruto, Michele Petrella, Maria Vittoria Micioni Di Bonaventura, Laura Soverchia, Serena Stopponi, Friedbert Weiss, Carlo Cifani, Roberto Ciccocioppo
Summary: Nociceptin/orphanin FQ (N/OFQ) is a neuropeptide that binds the NOP receptor and exerts anti-opioids effects; the NOP receptor is widely expressed in the brain, while N/OFQ is mainly localized in stress-related brain nuclei; preclinical evidence suggests that both NOP agonists and antagonists may be effective therapeutic approaches for substance use disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Amal El Daibani, Tao Che
Summary: In our society today, pain has become a significant source of strain on individuals. Developing new pain treatments with fewer adverse effects is crucial. The NOP receptor has emerged as a promising focal point for novel pain therapies, offering an alternative to opioid morphine with potential life-threatening effects and addiction. This article reviews the molecular and cellular structure controlling the cellular trafficking of the NOP receptor and presents studies supporting its role in pain management.
Review
Pharmacology & Pharmacy
Omar N. Al Yacoub, Hibah O. Awwad, Yong Zhang, Kelly M. Standifer
Summary: This article reviews the role of the N/OFQ-NOP receptor system in the neurological and behavioral sequelae of PTSD and TBI. The current findings suggest that NOP modulators may have potential as therapeutics for these disorders and their co-morbidities. The authors advocate for the development of standards for common data elements reporting to improve reproducibility and data sharing.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Pharmacology & Pharmacy
Mark F. Bird, Christopher P. Hebbes, Anushuya Tamang, Jonathon Mark Willets, Jonathan P. Thompson, Remo Guerrini, Girolamo Calo, David G. Lambert
Summary: In this study, the role of N/OFQ-NOP system was explored in freshly isolated volunteer human B- and T-cells incubated with LPS and PepG as a model of in vitro sepsis. The findings suggest that N/OFQ-NOP receptors play a regulatory role in B and T cell function, inhibiting cell migration and reducing GM-CSF release to variable degrees.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Omar N. Al Yacoub, Stefano Tarantini, Yong Zhang, Anna Csiszar, Kelly M. Standifer
Summary: Traumatic brain injury (TBI) affects millions of people each year and is a leading cause of death and disability. This study suggests that the N/OFQ-NOP receptor pathway plays a significant role in regulating cerebral blood flow and injury markers in TBI, indicating that the NOP receptor could be a potential therapeutic target for TBI.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Elaine C. Gavioli, Victor A. D. Holanda, Girolamo Calo, Chiara Ruzza
Summary: Resilience plays a crucial role in mental health, and understanding its mechanisms can improve treatment outcomes and help high-risk individuals. The N/OFQ-NOP system is central to stress response, affecting hormone secretion, behavior, and psychopathologies.
Article
Neurosciences
Sarah Sayers, Nikki Le, Jennifer Hernandez, Veronica Mata-Pacheco, Edward J. Wagner
Summary: N/OFQ(ARC) neurons play a vital role in promoting rebound hyperphagia by inhibiting anorexigenic neural substrates under negative energy balance conditions, an effect that is diminished by E-2 in females.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Chemistry, Multidisciplinary
Bin Li, Kejiao Gao, Yurong Li, Yuansheng Li, Longyi Zhu, Xuanyu Fu, Xiyong Zhuo, Ying Wu, Ying Wan, Shengyuan Deng
Summary: Recent studies have shown that enzymes undergo chemotaxis during catalysis. This study demonstrated the chemotaxis of zinc porphyrin as a cofactor mimic and its directed motions induced by ligand-protein binding. The dissociation constant for selected recognition was obtained by measuring the chemotactic shift, and a statistical thermodynamic model was derived to explain the directional migration of receptors.
Article
Chemistry, Analytical
Arcan Guven, Kayleigh Gray, Kuan-Wei Peng, Allison Klotz, Mark D. Kellogg, Niven R. Narain, Michael A. Kiebish
Summary: The validated NCAM-1 assay accurately quantifies NCAM-1 in plasma, meeting pre-determined assay validation parameters. The assay has potential to be used as a biomarker once appropriate clinical assessment is conducted and diagnostic thresholds are established.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Xiao-Long Liu, Zhe-Ran Wu, Wang Liao, Xiao-Qing Zhang, Yi-Wen Pei, Min Lu
Summary: This study investigated the function and binding properties of two general odorant binding proteins (GOBPs) in Spodoptera frugiperda. It was found that SfruGOBP1 had binding affinities to a few volatiles and insecticides, while SfruGOBP2 showed a broader ligand-binding spectrum, suggesting its importance in perceiving host volatiles. Molecular docking and site-directed mutagenesis assays provided insight into the key amino acid residues involved in the binding of insecticides to the GOBPs. This study offers valuable information for understanding the olfactory mechanism of GOBPs in S. frugiperda.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Editorial Material
Pharmacology & Pharmacy
David Fairman, Huaping Tang
Summary: Biologics, particularly monoclonal antibodies (mAbs), play an increasingly important role in drug discovery and development in the pharmaceutical industry. Specialized assays are needed to accurately measure the complex interactions between mAbs and their targets, especially for soluble target interactions. Investing resources into the development of total assays for both mAb and target is recommended for more robust data generation and analysis.
Article
Cell Biology
Munkhzaya Byambaragchaa, Ayoung Park, So-Jin Gil, Hae-Won Lee, Yun-Jeong Ko, Seung-Hee Choi, Myung-Hwa Kang, Kwan-Sik Min
Summary: By constructing mutant expression vectors targeting the eCG beta-subunit amino acid residues 102-104 and expressing them in CHO-K1 cells, it was found that these residues may play a crucial role in signal transduction through the rat FSH receptor. Mutants showed lower LH-like and FSH-like activities compared to wild-type eCG, with the double mutants exhibiting particularly low FSH-like activity.
ANIMAL CELLS AND SYSTEMS
(2021)
Article
Chemistry, Analytical
Madeleine Ramona Volz, Bjoern Moosmann
Summary: This article presents the development of a mass-spectrometry-based binding assay for determining the binding affinities of compounds at the human mu-opioid receptor. The assay has a short runtime, high sensitivity, and produces results consistent with radioactive binding assays. It can be used to determine the receptor binding affinities of newly emerging designer opioids.
ANALYTICA CHIMICA ACTA
(2022)
Article
Agriculture, Multidisciplinary
Xiao-Qing Zhang, Ding-Ze Mang, Hui Liao, Jia Ye, Jia-Li Qian, Shuang-Lin Dong, Ya-Nan Zhang, Peng He, Qing-He Zhang, Endang R. Purba, Long-Wa Zhang
Summary: Hyphantria cunea is an invasive pest species in China that uses type II sex pheromone components. In this study, the binding mechanisms of its sex pheromone components to their respective pheromone-binding proteins were explored. The results showed that HcunPBPs have different selectivities for pheromone components with major and minor structural differences, providing insight into the olfactory mechanism of moths using type II pheromone components.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemical Research Methods
Alissa Rauwerdink, Michael Benson, Allison Jayne, Sathyapriya Babu, Jessica St Charles, Amy Smith
Summary: Critical reagents play a crucial role in ligand-binding assays, with their quality and continuity being essential for assay performance. Batch changes can necessitate the development of new assay formats, highlighting the importance of close monitoring and management of critical reagents.
Article
Neurosciences
Patrycja Kleczkowska, Emmanuel Hermans, Piotr Kosson, Agnieszka Kowalczyk, Anna Lesniak, Karolina Pawlik, Engin Bojnik, Sandor Benyhe, Barbara Nowicka, Magdalena Bujalska-Zadrozny, Aleksandra Misicka, Andrzej W. Lipkowski
Article
Pharmacology & Pharmacy
Ferenc Zador, Mihaly Balogh, Andras Varadi, Zoltan S. Zadori, Kornel Kiraly, Edina Szucs, Bence Varga, Bernadette Lazar, Sandor Hosztafi, Pal Riba, Sandor Benyhe, Susanna Furst, Mahmoud Al-Khrasani
EUROPEAN JOURNAL OF PHARMACOLOGY
(2017)
Article
Pharmacology & Pharmacy
Ferenc Zador, Kornel Kiraly, Andras Varadi, Mihaly Balogh, Agnes Feher, Dora Kocsis, Anna I. Erdei, Erzsebet Lacko, Zoltan S. Zadori, Sandor Hosztafi, Bela Noszal, Pal Riba, Sandor Benyhe, Susanna Furst, Mahmoud Al-Khrasani
EUROPEAN JOURNAL OF PHARMACOLOGY
(2017)
Review
Biochemistry & Molecular Biology
Gabor Nagy-Grocz, Ferenc Zador, Szabolcs Dvoracsko, Zsuzsanna Bohar, Sandor Benyhe, Csaba Tomboly, Arpad Pardutz, Laszlo Vecsei
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
Article
Biochemistry & Molecular Biology
Adriano Mollica, Sveva Pelliccia, Valeria Famiglini, Azzurra Stefanucci, Giorgia Macedonio, Annalisa Chiavaroli, Giustino Orlando, Luigi Brunetti, Claudio Ferrante, Stefano Pieretti, Ettore Novellino, Sandor Benyhe, Ferenc Zador, Anna Erdei, Edina Szucs, Reza Samavati, Szalbolch Dvrorasko, Csaba Tomboly, Rino Ragno, Alexandros Patsilinakos, Romano Silvestri
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2017)
Article
Clinical Neurology
Reza Samavati, Ferenc Zador, Edina Szucs, Bernadett Tuka, Diana Martos, Gabor Veres, Robert Gaspar, Istvan M. Mandity, Ferenc Fulop, Laszlo Vecsei, Sandor Benyhe, Anna Borsodi
JOURNAL OF THE NEUROLOGICAL SCIENCES
(2017)
Article
Neurosciences
Ji Hoon Yoo, Anna Borsodi, Geza Toth, Sandor Benyhe, Robert Gaspar, Audrey Matifas, Brigitte L. Kieffer, Athanasios Metaxas, Ian Kitchen, Alexis Bailey
NEUROSCIENCE LETTERS
(2017)
Article
Chemistry, Medicinal
Azzurra Stefanucci, Ettore Novellino, Sako Mirzaie, Giorgia Macedonio, Stefano Pieretti, Paola Minosi, Edina Szucs, Anna I. Erdei, Ferenc Zador, Sandor Benyhe, Adriano Mollica
ACS MEDICINAL CHEMISTRY LETTERS
(2017)
Article
Chemistry, Medicinal
Azzurra Stefanucci, Alfonso Carotenuto, Giorgia Macedonio, Ettore Novellino, Stefano Pieretti, Francesca Marzoli, Edina Szucs, Anna I. Erdei, Ferenc Zador, Sandor Benyhe, Adriano Mollica
ACS MEDICINAL CHEMISTRY LETTERS
(2017)
Article
Cell Biology
Hadley C. Bergstrom, Anna M. Lipkin, Abby G. Lieberman, Courtney R. Pinard, Ozge Gunduz-Cinar, Emma T. Brockway, William W. Taylor, Mio Nonaka, Olena Bukalo, Tiffany A. Wills, F. Javier Rubio, Xuan Li, Charles L. Pickens, Danny G. Winder, Andrew Holmes
Article
Neurosciences
Sara Ferrazzo, Ozge Gunduz-Cinar, Nadia Stefanova, Gabrielle A. Pollack, Andrew Holmes, Claudia Schmuckermair, Francesco Ferraguti
NEUROBIOLOGY OF DISEASE
(2019)
Article
Biochemistry & Molecular Biology
Elliot D. Mock, Mohammed Mustafa, Ozge Gunduz-Cinar, Resat Cinar, Gavin N. Petrie, Vasudev Kantae, Xinyu Di, Daisuke Ogasawara, Zoltan V. Varga, Janos Paloczi, Cristina Miliano, Giulia Donvito, Annelot C. M. van Esbroeck, Anouk M. F. van der Gracht, Ioli Kotsogianni, Joshua K. Park, Andrea Martella, Tom van der Wel, Marjolein Soethoudt, Ming Jiang, Tiemen J. Wendel, Antonius P. A. Janssen, Alexander T. Bakker, Colleen M. Donovan, Laura Castillo, Bogdan Florea, Jesse Wat, Helma van den Hurk, Matthias Wittwer, Uwe Grether, Andrew Holmes, Constant A. A. van Boeckel, Thomas Hankemeier, Benjamin F. Cravatt, Matthew W. Buczynski, Matthew N. Hill, Pal Pacher, Aron H. Lichtman, Mario van der Stelt
NATURE CHEMICAL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Amir Mohammadzadeh, Peter P. Lakatos, Mihaly Balogh, Ferenc Zador, David arpad Karadi, Zoltan S. Zadori, Kornel Kiraly, Anna Rita Galambos, Szilvia Barsi, Pal Riba, Sandor Benyhe, Laszlo Koles, Tamas Tabi, Eva Szoko, Laszlo G. Harsing, Mahmoud Al-Khrasani
Summary: Animal studies suggest that GlyT inhibitors have potential as analgesics for neuropathic pain, but concerns about adverse effects remain. Combining GlyT-1 and GlyT-2 inhibitors can enhance analgesic effects, with increased CSF glycine content playing a key role.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Ferenc Zador, Samia Joca, Gabor Nagy-Grocz, Szabolcs Dvoracsko, Edina Szucs, Csaba Tomboly, Sandor Benyhe, Laszlo Vecsei
Summary: Substance use/abuse is a major cause of depression, potentially leading to inflammatory reactions triggering depressive symptoms, and increased pro-inflammatory cytokines may affect tryptophan metabolism, resulting in reduced serotonin levels, and subsequently inducing depression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Laura Rovira-Esteban, Ozge Gunduz-Cinar, Olena Bukalo, Aaron Limoges, Emma Brockway, Kinga Muller, Lief Fenno, Yoon Seok Kim, Charu Ramakrishnan, Tibor Andrasi, Karl Deisseroth, Andrew Holmes, Norbert Hajos
Article
Biochemistry & Molecular Biology
Hui-Ting Huang, Shun-Fen Tzeng
Summary: Our study demonstrates the role of interleukin-33 (IL-33) in a demyelinating mouse model induced by cuprizone (CPZ), showing that IL-33 can alleviate the reduction of APC+ OLs and the decline of IL-33 levels in the corpus callosum, and promote the expression of myelin basic protein (MBP).
NEUROCHEMISTRY INTERNATIONAL
(2024)