期刊
NEUROCHEMICAL RESEARCH
卷 36, 期 11, 页码 2186-2194出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11064-011-0543-x
关键词
Achyranthes bidentata polypeptides; Active fraction; Serum deprivation; Apoptosis; Akt/Gsk3 beta signaling; SH-SY5Y cells
资金
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- project for industrialization promotion of RD achievements in universities and colleges [JH09-33]
Achyranthes bidentata Blume is a commonly prescribed Chinese medicinal herb. Our previous studies have proved the neuroprotective function of Achyranthes bidentata polypeptides (ABPP), a major constituent from aqueous extracts of the herb. Now we have separated an active fraction, referred to as ABPP-E4, from ABPP by HPLC methods. This study aimed to investigate the possible therapeutic potential of ABPP-E4. Assessments of cell viability and apoptosis indicated that ABPP-E4 pretreatment, in a concentration-dependent manner, antagonized the cell viability loss and cell apoptosis of cultured SH-SY5Y cells deprived of serum. ABPP-E4 pretreatment also resulted in increase of Bcl-2/Bax ratio and inhibition of caspase-3 activation in the cells on exposure to serum deprivation. Signaling pathway analysis indicated that ABPP-E4 treatment stimulated the activation of Akt/Gsk3 beta signaling in cultured SH-SY5Y cells, and anti-apoptotic effects of ABPP-E4 could be blocked by chemical inhibition of PI3K. Taken together, all the results suggest that ABPP-E4 might exert protective effects against serum deprivation-induced neuronal apoptosis through modulation of PI3K/Akt/Gsk3 beta pathways.
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