期刊
NEUROBIOLOGY OF AGING
卷 31, 期 12, 页码 2080-2090出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.neurobiolaging.2008.11.003
关键词
Inclusion body myositis; beta-Amyloid; beta APP; Ryanodine receptors; Excitation-contraction coupling
资金
- NIH [K01AR053114, R03AR054519]
Inclusion body myositis (IBM), the most common muscle disorder in the elderly, is partly characterized by dysregulation of beta-amyloid precursor protein (beta APP) expression and abnormal, intracellular accumulation of full-length beta APP and beta-amyloid epitopes. The present study examined the effects of beta-amyloid accumulation on force generation and Ca2+ release in skeletal muscle from transgenic mice harboring human beta APP and assessed the consequence of A beta(1-42) modulation of the ryanodine receptor Ca2+ release channels (RyRs). beta-Amyloid laden muscle produced less peak force and exhibited Ca2+ transients with smaller amplitude. To determine whether modification of RyRs by beta-amyloid underlie the effects observed in muscle, in vitro Ca2+ release assays and RyR reconstituted in planar lipid bilayer experiments were conducted in the presence of A beta(1-42). Application of A beta(1-42) to RyRs in bilayers resulted in an increased channel open probability and changes in gating kinetics, while addition of A beta(1-42) to the rabbit SR vesicles resulted in RyR-mediated Ca2+ release. These data may relate altered BAPP metabolism in IBM to reductions in RyR-mediated Ca2+ release and muscle contractility. (C) 2008 Elsevier Inc. All rights reserved.
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