期刊
NEUROBIOLOGY OF AGING
卷 29, 期 8, 页码 1256-1264出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.neurobiolaging.2007.02.013
关键词
hippocampus; spinophilin; rat; aging; Morris water-maze; learning; memory; stereology
资金
- NIA NIH HHS [F32 AG005868, AG 05868, P01 AG009973, AG 09973, P01 AG009973-09] Funding Source: Medline
Age-related impairments in hippocampus-dependent learning and memory tasks are not associated with a loss of hippocampal neurons, but may be related to alterations in synaptic integrity. Here we used stereological techniques to estimate spine number in hippocampal subfields using immunostaining for the spine-associated protein, spinophilin, as a marker. Quantification of the immunoreactive profiles was performed using the optical disector/fractionator technique. Aging was associated with a modest increase in spine number in the molecular layer of the dentate gyrus and CA1 stratum lacunosum-moleculare. By comparison, spinophilin protein levels in the hippocampus, measured by Western blot analysis, failed to differ as a function of age. Neither the morphological nor the protein level data were correlated with spatial learning ability across individual aged rats. The results extend current evidence on synaptic integrity in the aged brain, indicating that a substantial loss of dendritic spines and spinophilin protein in the hippocampus are unlikely to contribute to age-related impairment in spatial learning. (c) 2007 Elsevier Inc. All rights reserved.
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