Article
Biochemistry & Molecular Biology
Patrick C. Baer, Ann-Kathrin Neuhoff, Ralf Schubert
Summary: Renal proximal tubular epithelial cells (PTCs) play a central role in renal inflammation. In response to inflammation, PTCs undergo changes in gene expression and release extracellular vesicles (EVs) containing inflammation-specific cargo molecules. Understanding the molecular mechanisms of kidney inflammation is crucial for identifying targets in the prevention or treatment of acute kidney injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Benjamin Koch, Dominik C. Fuhrmann, Ralf Schubert, Helmut Geiger, Thimoteus Speer, Patrick C. Baer
Summary: Inflammation is closely related to the development of diabetic kidney disease. Gliflozins, as SGLT-2 inhibitors, have been shown to reduce inflammation and slow down the progression of diabetic nephropathy. In this study using human renal proximal tubular epithelial cells, gliflozins were found to have an anti-inflammatory effect by inhibiting the expression of pro-inflammatory factors and the adhesion molecule ICAM-1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Min Chen, Xiangchen Gu
Summary: Endocytosis plays a crucial role in kidney diseases, particularly in renal fibrosis, and is affected by other diseases and factors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Urology & Nephrology
Tadej Petreski, Luka Varda, Lidija Gradisnik, Uros Maver, Sebastjan Bevc
Summary: The kidneys are the primary excretion organ with various functions, and the nephron is their central structural unit. The treatment of acute kidney injury or chronic kidney disease is complex due to limited regeneration potential. The development of in vitro cell models based on patient-derived renal cells provides an opportunity to address issues related to drug-drug interactions and complications.
Review
Physiology
Andrew M. Hall, Sophie de Seigneux
Summary: Damage to the proximal tubule is the most common cause of acute kidney injury in humans. Current diagnostic and treatment options are limited, and a deeper understanding of pathogenic mechanisms at a cellular level is needed. Recent studies have shown significant changes in proximal tubule metabolism during acute kidney injury, highlighting potential targets for intervention. This article emphasizes the current state of the field and highlights three emerging areas worthy of attention: axial heterogeneity in determining baseline susceptibility, insult specific pathogenic mechanisms, and the link between tubular metabolism and whole-body homeostasis.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2022)
Article
Immunology
Samuel Shin, Eugenia Awuah Boadi, Saloni Shah, Madison Ezell, Peijun Li, Bidhan C. Bandyopadhyay
Summary: Renal tubular epithelial cells can synthesize interleukins in response to inflammation, and elevated levels of interleukins have been found in patients with nephritic diseases. This study shows that L-arginine plays an immunological signaling role in renal tubular epithelial cells through the activation of CaSR and TRPC channel. This signaling pathway has a potential protective effect on the kidneys.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Review
Nutrition & Dietetics
Leslie S. Gewin
Summary: The kidney relies on specialized epithelial cells in the renal tubules for reabsorption, with the proximal tubules using fatty acid oxidation as the preferred metabolic pathway. After kidney injury, there are alterations in metabolism leading to decreased fatty acid oxidation and increased lactic acid generation.
Article
Biochemistry & Molecular Biology
Chung-Kuan Wu, Chia-Lin Wu, Tzong-Shyuan Lee, Yu Ru Kou, Der-Cherng Tarng
Summary: The study found that TRPA1 plays a role in AKI by sensing oxidative stress and regulating inflammation in renal tubular cells. TRPA1 expression increased in patients with AKI and in a mouse model of IR-induced injury, highlighting its importance in kidney injury. In vitro experiments showed that TRPA1 activation by ROS under hypoxia-reoxygenation injury conditions led to increased intracellular Ca2+ level and activation of inflammatory signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Katharina Schmalkuche, Reinhard Schwinzer, Nadine Wenzel, Emilio Valdivia, Bjoern Petersen, Rainer Blasczyk, Constanca Figueiredo
Summary: This study demonstrated the feasibility of generating highly proliferative PTECs and developing tissue-specific immunomonitoring assays. Silencing SLA expression on PTECs was shown to be an effective strategy to prevent xenogeneic cellular immune responses and may strongly support graft survival after xenotransplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Dan Wang, Snehal Sant, Craig Lawless, Nicholas Ferrell
Summary: This study introduces a cell culture system that uses in vivo-derived basement membrane to investigate the interactions between kidney tubular cells and the extracellular matrix. The study shows that the mechanical properties of the basement membrane affect the function and gene expression of tubular epithelial cells.
INTEGRATIVE BIOLOGY
(2022)
Article
Urology & Nephrology
Jiafa Ren, Kang Liu, Buyun Wu, Xiaohan Lu, Lianqin Sun, Jamie R. Privratsky, Changying Xing, Matthew J. Robson, Huijuan Mao, Randy D. Blakely, Koki Abe, Tomokazu Souma, Steven D. Crowley
Summary: This study examines the expression of IL-1 and IL-1R1 in renal cells and infiltrating immune cells during AKI and demonstrates opposing effects of IL-1 receptor signaling on AKI. Disrupting IL-1R1 activation in tubules while preserving IL-1R1 activation in endothelial cells may provide better renal protection.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Article
Oncology
Jennifer Eymael, Martijn van den Broek, Laura Miesen, Valerie Villacorta Monge, Bartholomeus T. van den Berge, Fieke Mooren, Vicky Luna Velez, Jelmer Dijkstra, Meyke Hermsen, Peter Bandi, Michiel Vermeulen, Saskia de Wildt, Brigith Willemsen, Sandrine Florquin, Roy Wetzels, Eric Steenbergen, Rafael Kramann, Marcus Moeller, Michiel F. Schreuder, Jack Fm Wetzels, Johan van der Vlag, Jitske Jansen, Bart Smeets
Summary: Scattered tubular cells (STCs) in the proximal tubule increase in number after acute kidney injury. STCs are preferentially located within the inner bends of the tubule and their number increases with age. STCs represent a transient state of dedifferentiated proximal tubule epithelial cells (PTECs) showing metabolic shift towards glycolysis, facilitating cellular survival after kidney injury.
JOURNAL OF PATHOLOGY
(2023)
Article
Cell Biology
Muhammad Ali Khan, Purba Nag, Anca Grivei, Kurt T. K. Giuliani, Xiangju Wang, Vishal Diwan, Wendy Hoy, Helen Healy, Glenda Gobe, Andrew J. Kassianos
Summary: The pathogenesis of crystal nephropathy involves deposition of intratubular crystals, tubular obstruction and cell death. This study investigated the modes of adenine-induced tubular cell death and found that baicalein can act as a novel cell death inhibitor. Baicalein inhibits cell death by modulating the mitochondrial antioxidant enzyme SOD2.
CELL DEATH & DISEASE
(2022)
Article
Medicine, Research & Experimental
Glory Ranches, Maximilian Zeidler, Roman Kessler, Martina Hoelzl, Michael W. Hess, Jonathan Vosper, Paul Perco, Herbert Schramek, Kai K. Kummer, Michaela Kress, Anne Krogsdam, Michael Rudnicki, Gert Mayer, Alexander Huettenhofer
Summary: Exosomes are valuable biomarkers for studying chronic kidney disease (CKD). Urinary exosomes and exosomes derived from renal proximal tubular epithelial cells (RPTECs) were analyzed for microRNA (miRNA) profiles. Dysregulated exosomal miRNAs associated with pro-inflammatory and pro-fibrotic pathways were identified in RPTECs, suggesting their potential use in predicting CKD pathogenesis.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Endocrinology & Metabolism
Zhimin Wang, Jiating Yu, Dan Hao, Xin Liu, Xiao Wang
Summary: This study investigated the differential gene expression and gene relationships in renal tubular cells under high glucose conditions with PKM2 tetramerization activation, identifying key gene candidates and pathways related to diabetic kidney disease. The findings suggest a potential role of PKM2 in reshaping cellular interactions and signaling pathways to promote resilience in response to metabolic stress.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Medicine, General & Internal
Markus Ketteler, Geoffrey A. Block, Pieter Evenepoel, Masafumi Fukagawa, Charles A. Herzog, Linda McCann, Sharon M. Moe, Rukshana Shroff, Marcello A. Tonelli, Nigel D. Toussaint, Marc G. Vervloet, Mary B. Leonard
ANNALS OF INTERNAL MEDICINE
(2018)
Article
Cardiac & Cardiovascular Systems
Csaba P. Kovesdy, Kunihiro Matsushita, Yingying Sang, Nigel J. Brunskill, Juan J. Carrero, Gabriel Chodick, Takeshi Hasegawa, Hiddo L. Heerspink, Atsushi Hirayama, Gijs W. D. Landman, Adeera Levin, Dorothea Nitsch, David C. Wheeler, Josef Coresh, Stein I. Hallan, Varda Shalev, Morgan E. Grams
EUROPEAN HEART JOURNAL
(2018)
Article
Transplantation
Hirotaka Komaba, Takatoshi Kakuta, Takehiko Wada, Miho Hida, Takao Suga, Masafumi Fukagawa
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2019)
Article
Multidisciplinary Sciences
Takehisa Kawata, Shin Tokunaga, Miki Murai, Nami Masuda, Waka Haruyama, Youji Shoukei, Yutaka Hisada, Tetsuya Yanagida, Hiroshi Miyazaki, Michihito Wada, Tadao Akizawa, Masafumi Fukagawa
Article
Urology & Nephrology
Takashi Shigematsu, Ryutaro Shimazaki, Masafumi Fukagawa, Tadao Akizawa
CLINICAL AND EXPERIMENTAL NEPHROLOGY
(2019)
Article
Medicine, Research & Experimental
Kazuya Narushima, Hiroshi Maeda, Masanari Shiramoto, Yuichi Endo, Satoko Ohtsuka, Hiroaki Nakamura, Yoshinori Nagata, Tatsuo Uchimura, Ayako Kannami, Ryutaro Shimazaki, Masafumi Fukagawa, Tadao Akizawa
CTS-CLINICAL AND TRANSLATIONAL SCIENCE
(2019)
Review
Biochemistry & Molecular Biology
Yoshiko Iwasaki, Hideyuki Yamato, Masafumi Fukagawa
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Urology & Nephrology
Masafumi Fukagawa, Ryutaro Shimazaki, Tadao Akizawa
KIDNEY INTERNATIONAL
(2018)
Article
Food Science & Technology
Takehiro Nakano, Hiroshi Watanabe, Tadashi Imafuku, Kai Tokumaru, Issei Fujita, Nanaka Arimura, Hitoshi Maeda, Motoko Tanaka, Kazutaka Matsushita, Masafumi Fukagawa, Toru Maruyama
Summary: Indoxyl sulfate (IS) activates mTORC1, promoting renal fibrosis; IS also induces epithelial-mesenchymal transition and inflammatory response, which are associated with renal fibrosis; Therapeutic interventions targeting IS and mTORC1 could be effective against renal fibrosis in CKD.
Article
Medicine, General & Internal
Masao Iwagami, Yuka Kanemura, Naru Morita, Toshitaka Yajima, Masafumi Fukagawa, Shuzo Kobayashi
Summary: This study examined hemodialysis patients in Japan with dyskalemia and analyzed their characteristics and clinical outcomes. The study found that dyskalemia was associated with adverse outcomes such as increased mortality and major adverse cardiovascular events. Older age and malnutrition were associated with hypokalemia, while a longer duration of dialysis was associated with hyperkalemia. Optimizing serum potassium levels is important for hemodialysis patients.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Nobumichi Saito, Masao Toyoda, Masumi Kondo, Makiko Abe, Noriyuki Sanechika, Moritsugu Kimura, Kaichiro Sawada, Masafumi Fukagawa
Summary: This study reveals that phosphorylation of β1-integrin inhibits the expression of renin, while dephosphorylation promotes its expression. Knockdown of β1-integrin and connexin-40 both enhance the expression of renin. Pneumatic pressurization and hypotonic medium culture decrease renin expression, which can be restored by β1-integrin knockdown.
Article
Pharmacology & Pharmacy
Takashi Shigematsu, Ryutaro Shimazaki, Masafumi Fukagawa, Tadao Akizawa
CLINICAL PHARMACOLOGY-ADVANCES AND APPLICATIONS
(2018)
Article
Urology & Nephrology
Takashi Shigematsu, Masafumi Fukagawa, Keitaro Yokoyama, Takashi Akiba, Akifumi Fujii, Motoi Odani, Tadao Akizawa
CLINICAL AND EXPERIMENTAL NEPHROLOGY
(2018)
Article
Urology & Nephrology
Masaaki Nakayama, Yoshihiro Tani, Wan-Jun Zhu, Kimio Watanabe, Keitaro Yokoyama, Masafumi Fukagawa, Takashi Akiba, Myles Wolf, Hideki Hirakata
KIDNEY INTERNATIONAL REPORTS
(2018)
Article
Urology & Nephrology
Fumihiko Koiwa, Keitaro Yokoyama, Masafumi Fukagawa, Tadao Akizawa
KIDNEY INTERNATIONAL REPORTS
(2018)