Article
Multidisciplinary Sciences
Harim Oh, Chul Hwan Kim, Yoo Jin Lee
Summary: The study evaluated the diagnostic value of immunohistochemical staining for bullous pemphigoid (BP) detection, showing that IHC staining has high sensitivity and specificity, being helpful for the diagnosis of DIF-confirmed BP cases and clinically suspected BP cases.
SCIENTIFIC REPORTS
(2022)
Article
Medical Laboratory Technology
Jean-Baptiste Gibier, Romain Perbet, Benjamin Lopez, Magali Colombat, Romain Dubois, Sarah Humez, Louis Terriou, Marie-Christine Copin, Viviane Gnemmi
Summary: Differentiating between types of amyloid is crucial for treatment and outcomes. Paraffin immunofluorescence shows promise in detecting light-chain amyloidosis and is a valuable addition to routine workflow.
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE
(2021)
Article
Chemistry, Analytical
Jie Pu, Chao Xue, Shihan Huo, Qingqing Shen, Yang Qu, Xinxin Yang, Bo An, Thomas E. Angel, Zhuo Chen, John T. Mehl, Huaping Tang, Eric Yang, Timothy W. Sikorski, Jun Qu
Summary: This study extensively investigated the challenges of accurate quantification of target proteins in formalin-fixed paraffin-embedded (FFPE) tissues using liquid chromatography-mass spectrometry (LC-MS) and developed a strategy enabling precise quantification. The study demonstrated efficient recovery of target signature peptides from FFPE tissues, indicated that FFPE tissue storage mainly causes decreased immunoreactivity but not chemical degradation of proteins, and proposed a novel calibration approach for accurate quantification. The pipeline was successfully applied to measure the pharmacokinetics of monoclonal antibody (mAb) and its target in FFPE tissues, showing excellent correlation with fresh tissues.
ANALYTICAL CHEMISTRY
(2022)
Article
Medicine, General & Internal
Nipan Das, Rennie O. Lakadong, Biswajit Dey, Vandana Raphael
Summary: The diagnostic utility of paraffin immunofluorescence by proteinase K digestion on renal biopsy compared to fresh frozen immunofluorescence was assessed. Differences in antibody intensity were noted mainly in lupus nephritis, IgA nephropathy, and membranoproliferative glomerulonephritis. Paraffin immunofluorescence by proteinase K digestion showed high sensitivity and specificity in kidney biopsies.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2023)
Article
Medical Laboratory Technology
Nina Ogrinc, Pierre-Damien Caux, Yves-Marie Robin, Emmanuel Bouchaert, Benoit Fatou, Michael Ziskind, Cristian Focsa, Delphine Bertin, Dominique Tierny, Zoltan Takats, Michel Salzet, Isabelle Fournier
Summary: A novel method for analyzing tissue-lipidomic signatures directly from FFPE tissues without extensive sample preparation was proposed in this study, showing promising results for accurate classification of sarcoma subtypes.
CLINICAL CHEMISTRY
(2021)
Article
Dermatology
Wenzhe Zhao, Haiqin Zhu, Xiaoqing Zhao, Xinyi Wu, Fei Sun, Meng Pan, Shengru Zhou
Summary: The application value of DIF-P detecting IgG using HIAR in the diagnosis of bullous dermatosis was explored. The study concluded that DIF-P can be used as an alternative method for the diagnosis of pemphigus.
CLINICAL COSMETIC AND INVESTIGATIONAL DERMATOLOGY
(2023)
Article
Biochemical Research Methods
Patcharaporn Boottanun, Chiaki Nagai-Okatani, Misugi Nagai, Umbhorn Ungkulpasvich, Shinjiro Yamane, Masao Yamada, Atsushi Kuno
Summary: The researchers evaluated the sensitivity of a new scanner for analyzing glycans attached to glycoproteins in biological samples. The scanner showed fourfold higher sensitivity compared to the previous scanner and allowed glycomic profiling with only three cells. It also enabled high-resolution spatial analysis in tissue glycome mapping.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Cell Biology
Federica Grillo, Michela Campora, Simona Pigozzi, Silvia Bonadio, Luca Valle, Jacopo Ferro, Michele Paudice, Beatrice Dose, Luca Mastracci
Summary: This study aimed to identify methods to improve Ki67 immunohistochemical reactions in old archival FFPE blocks. The results indicated that deep sectioning and prolonged heat-based pretreatment significantly increased immunoreactivity for Ki67, while re-embedding and reprocessing did not have a significant effect. Additionally, large tissue samples exhibited heterogeneity of Ki67 immunoexpression between the periphery and central area.
HISTOCHEMISTRY AND CELL BIOLOGY
(2021)
Article
Microbiology
Alexis Trecourt, Meja Rabodonirina, Claire Mauduit, Alexandra Traverse-Glehen, Mojgan Devouassoux-Shisheboran, David Meyronet, Frederique Dijoud, Christophe Ginevra, Emmanuelle Chapey-Picq, Emilie Josse, Patricia Martins-Simoes, Abderrazzak Bentaher, Damien Dupont, Charline Miossec, Florence Persat, Martine Wallon, Tristan Ferry, Felix Pham, Bruno Simon, Jean Menotti
Summary: A targeted next-generation sequencing (NGS) method was developed to achieve a fungal integrated histomolecular diagnosis using formalin-fixed tissue samples (FTs), improving the accuracy of fungal detection and identification.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Integrative & Complementary Medicine
Jing Wei, Tianyu Chen, Xiaolin Chen, Ganzhu Feng
Summary: In this study, the diagnostic value of three different tests for tuberculous pleurisy (TP) was compared, and a new test called paraffin-embedded sample test (PEST) was found to have a high sensitivity and overall diagnostic value for TP diagnosis.
ALTERNATIVE THERAPIES IN HEALTH AND MEDICINE
(2023)
Article
Veterinary Sciences
Raul Leal Faria Luiz, Rodrigo Caldas Menezes, Sandro Antonio Pereira, Raquel de Vasconcellos Carvalhaes de Oliveira, Manoel Marques Evangelista Oliveira
Summary: Sporotrichosis is a chronic fungal disease that affects humans and animals. This study aimed to evaluate and validate a nested PCR technique for detecting Sporothrix sp. DNA from formalin-fixed and paraffin-embedded tissues (FFPE). The results showed that the chemical extraction protocol with 5μm thick paraffin sections was the most effective in extracting DNA from Sporothrix sp. The nested PCR technique exhibited high sensitivity and specificity for detecting Sporothrix sp. in FFPE samples.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Microbiology
Robert J. Lauerer, Emely Rosenow, Rudi Beschorner, Johann-Martin Hempel, Georgios Naros, Anna Hofmann, Katharina Berger, Jennifer Sartor-Pfeiffer, Annerose Mengel, Ulf Ziemann, Volker Rickerts, Katharina Feil
Summary: This study investigates brain abscesses caused by S. apiospermum, highlighting the challenges in diagnosing and treating this condition. The study finds that using specific quantitative PCR can facilitate early identification of fungi and potentially improve patient clinical outcomes.
Article
Biochemical Research Methods
Maddalena M. Bolognesi, Francesco Mascadri, Laura Furia, Mario Faretta, Francesca M. Bosisio, Giorgio Cattoretti
Summary: Immunostaining on routinely processed tissue requires thorough antibody validation, but applying validated antibodies to frozen tissue sections may lead to unpredictable changes in performance. Results show that less than 30% of antibodies perform as expected with all fixation methods, implying a need for revalidation of established antibody panels when applied to sections with different fixation and processing methods.
Article
Parasitology
Jenny Knapp, Severine Lallemand, Franck Monnien, Sophie Felix, Severine Valmary-Degano, Sandra Courquet, Florent Demonmerot, Bruno Heyd, Celia Turco, Alexandre Doussot, Lucie Bourgeois, Solange Bresson-Hadni, Carine Richou, Laurence Millon
Summary: Confirmed diagnosis of alveolar echinococcosis (AE) relies on pathological criteria and molecular evidence. This study evaluated various PCR techniques for the diagnosis of AE in patients and identified the most effective strategy for molecular diagnosis based on the nature of the tested samples. The results showed that a qPCR assay targeting mitochondrial genes exhibited higher sensitivity in detecting AE samples.
Article
Dermatology
Colleen Lysen, Luciana Silva-Flannery, Sherif R. Zaki, Joy M. Gary, Shawn R. Lockhart
Summary: The study validates PCR from FFPE tissues as a reference laboratory identification methodology in the absence of abundant gold standard specimens. By comparing PCR results with other diagnostic methods, the study outlines the excellent performance of PCR from FFPE tissue, despite its relatively low sensitivity when compared to histopathology.