Article
Plant Sciences
Liu Zhang, Ryota Kawaguchi, Takuo Enomoto, Sho Nishida, Meike Burow, Akiko Maruyama-Nakashita
Summary: Based on our previous findings, glucosinolates (GSLs) can act as a sulfur source in plants via GSL catabolism. However, the contribution of BGLU28/30-dependent GSL catabolism during the reproductive growth stage remains unclear due to the variation in GSL profiles. Therefore, in this study, we examined the growth, metabolic, and transcriptional phenotypes of mature bglu28/30 mutants grown under different sulfur conditions. Our results showed impaired growth, increased GSL levels in reproductive organs and leaves, decreased primary sulfur-containing metabolites, and enhanced GSL transport in the bglu28/30 mutants under sulfur deficiency. Transcriptome analysis revealed differential responses to sulfur deficiency in genes related to ethylene and defense responses, as well as plant response to heat. These findings contribute to our understanding of the role of BGLU28/30-dependent GSL catabolism in plant adaptation to nutrient stress.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Agricultural Engineering
Daniel Eugui, Pablo Velasco, Patricia Abril-Urias, Carolina Escobar, Oscar Gomez-Torres, Sara Caballero, Jorge Poveda
Summary: This study aimed to standardize a protocol for obtaining a bioactive extract from broccoli crop residues that can be used on an industrial scale and effectively reduce plant disease incidence. The extracts showed biopesticidal activity against plant pathogenic fungi, but had no obvious effect on plant-parasitic nematodes.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Chemistry, Applied
Yu-Hsiang Wu, Yan-Han Lin, Chung-Yi Wang
Summary: The effects of high-pressure processing (HPP) on kale leaves were studied, including myrosinase activity, glucosinolate (GLS) content, isothiocyanate (ITC) conversion rate, color, and bacterial count. The sample processed at 600 MPa showed the highest myrosinase activity and ITC conversion rate, but the GLS content was significantly lower. HPP induced crushing damage to the leaves, promoting the conversion process, and caused minimal color change. HPP achieved the same level of pasteurization as thermal treatment in terms of bacterial count.
Article
Agriculture, Multidisciplinary
Yu Tie, Wenyou Zhu, Chao Zhang, Liguo Yin, Yalin Zhang, Linpei Liu, Huawei Yuan
Summary: In this study, a bacterial strain identified as Leclercia adecarboxylata was isolated from the rhizosphere soil of rape seedlings, which harbored two myrosinase genes and an ITC hydrolase gene. It was demonstrated that these myrosinases play a crucial role in the utilization of GSLs, and the metallo-beta-lactamase 3836 may be involved in the bacterium's tolerance mechanism to allyl-ITC.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agronomy
Jie Li, Hong-Mei Qian, Li-Long Pan, Qiao-Mei Wang, Shu-Sheng Liu
Summary: Glucosinolates appear to have little impact on the oviposition, survival, and development of immature stages of whiteflies MEAM1 and Asia II 3. The differences in colonization of Brassica crops by the two whitefly species are likely influenced by plant traits other than glucosinolates.
PEST MANAGEMENT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Giuliano Cutolo, Blanka Didak, Josip Tomas, Benoit Roubinet, Pierre Lafite, Reine Nehm, Marie Schuler, Ludovic Landemarre, Arnaud Tatibouet
Summary: A convenient 'one-pot' synthesis strategy for neoglycoproteins (NGP) was developed using myrosinaseglucosinolate couple, allowing the generation of multivalent glycoproteins without the use of harmful chemical reagents. Lectin binding screening showed that the NGPs synthesized exhibited similar or better affinities towards mannose-specific lectins compared to commercial approaches.
CARBOHYDRATE RESEARCH
(2022)
Article
Plant Sciences
Jiaxuan Chen, Zeyuan Chen, Zunwen Li, Yijiao Zhao, Xiaodong Chen, Gefu Wang-Pruski, Rongfang Guo
Summary: The study revealed that a 16-hour photoperiod under red-and-blue (RB) light had a positive impact on the growth of Chinese kale sprouts and accumulation of glucosinolates. Sprouts under blue light showed higher glucosinolate content compared to those under red light. The differential accumulation of glucosinolates under different light conditions was attributed to variations in biosynthetic gene transcripts and expression levels of genes related to glucosinolate degradation.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Chemistry, Applied
Xinxing Xu, Shuang Bi, Fei Lao, Fang Chen, Xiaojun Liao, Jihong Wu
Summary: The study investigated the metabolism of bioactive compounds in broccoli juice fermented by animal- and plant-derived Pediococcus pentosaceus. Results showed that P. pentosaceus may potentially improve the nutritional and functional properties of fermented foods.
Article
Agriculture, Multidisciplinary
Laura Plaza-Vinuesa, Oswaldo Hernandez-Hernandez, Ana Sanchez-Arroyo, Jose M. Cumella, Nieves Corzo, Ana M. Munnoz-Labrador, F. Javier Moreno, Blanca de las Rivas, Rosario Munoz
Summary: This study confirmed the myrosinase-like activity of Lactiplantibacillus plantarum WCFS1, indicating that glucosinolates in this strain may undergo phosphorylation and the glycosidases belonging to the GH1 family are involved in the hydrolysis of phosphorylated glucosinolates.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Vanda Pucikova, Sascha Rohn, Franziska S. Hanschen
Summary: Leaf age significantly affects the accumulation and hydrolysis of glucosinolates and their hydrolysis products in two leafy Brassica vegetables. Young leaves contain more than 3-fold more glucosinolates and release more isothiocyanates compared to older ones. Myrosinases hydrolyze all amounts of glucosinolates regardless of leaf age. Moreover, older leaves supply younger leaves with glucosinolates. Therefore, incorporating young leaves of leafy Brassicas in the diet can enhance the nutritional value of food.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Food Science & Technology
Villayat Ali, Manu Khajuria, Rohini Bhat, Aatif Rashid, Sheenam Faiz, Dhiraj Vyas
Summary: Lepidium latifolium L. sprouts contain a unique combination of 2-propenyl and benzyl glucosinolates, with the 3rd-week sprouts showing superior phytochemical properties compared to other stages. The high myrosinase activity during the 3rd-week may enhance the bioavailability of glucosinolates. Additionally, the antioxidant potential of the sprouts is positively correlated with the content of phenols and flavonoids, being higher during the first three weeks.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Han Qin, Graham J. King, Priyakshee Borpatragohain, Jun Zou
Summary: Glucosinolates (GSLs), a well-studied class of secondary metabolites mainly found in Brassicaceae family, have potential in plant defense and crop quality, but can also pose risks. This article explores the bioactive functions of GSLs, summarizes their diversity and distribution, and discusses the potential for genetic engineering to optimize their production in crops.
PLANT COMMUNICATIONS
(2023)
Article
Ecology
Jeanne Friedrichs, Rabea Schweiger, Svenja Geisler, Judith M. Neumann, Sullivan J. M. Sadzik, Karsten Niehaus, Caroline Mueller
Summary: The study found that the polyphagous tansy leaf beetle developed fastest on a cabbage diet and slowest on a tansy diet. Cabbage had the lowest water content, while tansy had the highest water content, C/N ratio, and trichome density, and the lowest specific leaf area.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2022)
Article
Biochemistry & Molecular Biology
Fatma Cebeci, Melinda J. Mayer, John T. Rossiter, Richard Mithen, Arjan Narbad
Summary: This study successfully isolated a Citrobacter strain with myrosinase activity from soil, purified and expressed the enzyme, and obtained its amino acid sequence and gene sequence. The heterologous expression of the myrosinase gene allowed for the production of a relatively large amount of myrosinase. The kinetic parameters and substrate specificity of the purified myrosinase were determined, providing a basis for further research on this enzyme.
Review
Soil Science
Daniel Eugui, Carolina Escobar, Pablo Velasco, Jorge Poveda
Summary: Plant-parasitic nematodes cause serious losses to crops worldwide, and traditional control methods are limited. Plant secondary metabolites, such as glucosinolates and glucosinolate hydrolysis products, show promising nematicidal activity and are presented as a sustainable alternative for nematode control.
APPLIED SOIL ECOLOGY
(2022)
Article
Multidisciplinary Sciences
Jeanne Friedrichs, Rabea Schweiger, Caroline Mueller
Summary: This study investigated the metabolic pathways and products of different glucosinolates in the herbivorous insect Phaedon cochleariae. The results showed differences in metabolism between adults and larvae, with metabolism primarily occurring via isothiocyanates. This detoxification strategy through conjugation with amino acids is unique to Brassicaceae-feeders.
SCIENTIFIC REPORTS
(2022)
Article
Agriculture, Multidisciplinary
Ann-Cathrin Voss, Elisabeth J. Eilers, Caroline Muller
Summary: Numerous pesticides are applied to crop plants annually, including fungicides, which may affect plant metabolism and crop quality. This study investigated the effects of two fungicides on the metabolism of strawberry cultivars and wild strawberries, specifically focusing on primary and specialized metabolites that contribute to fruit flavor. The fungicide treatment significantly impacted several metabolites, indicating potential changes in taste and quality of strawberries when treated with fungicides.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Lai Ka Lo, R. Reshma, Lisa Johanna Tewes, Barbara Milutinovic, Caroline Mueller, Joachim Kurtz
Summary: Group-living individuals face high risks of disease transmission and parasite infection. Research has found that in social and some non-social insects, immune control is achieved not only through individual immune defenses, but also through infochemicals such as contact cues and defensive volatiles, which help mount a group-level immunity. However, it is still not well understood whether activation of the immune system leads to changes in chemical phenotypes that mediate these responses.
JOURNAL OF CHEMICAL ECOLOGY
(2023)
Article
Plant Sciences
Martin Aguirrebengoa, Caroline Mueller, Peter A. Hamback, Adela Gonzalez-Megias
Summary: Plants have to regulate their responses to multiple herbivores to deal with a wide range of threats. In particular, they often face simultaneous root and floral herbivory, but few studies have focused on this interaction. This study investigated the combined density-dependent effects of root-chewing beetle larvae and flower-chewing caterpillars on the fitness and defense of a semiarid Brassicaceae herb. The results showed that the impact of both herbivore groups on plant fitness was independent and density-dependent. Increasing root herbivore density had a non-significant effect on plant fitness, while increasing floral herbivore density had a non-linear negative effect on seed number and seedling emergence. Plant defensive response was non-additive and varied with the densities of root and floral herbivores, showing compensatory investment in reproduction at high floral herbivore density and aboveground chemical defense induction at high root herbivore density.
Article
Plant Sciences
Rohit Sasidharan, Robert R. Junker, Elisabeth J. Eilers, Caroline Mueller
Summary: Plants use floral displays to attract mutually beneficial organisms and deter attackers. These displays include attractive or repellent floral volatile organic compounds (FVOCs) that can be detected from a distance. Locally, visitors perceive contact chemicals including nutrients and deterrent or toxic constituents of pollen and nectar. This review examines the composition of FVOCs and non-volatile floral chemicals, such as pollen nutrients and toxins, and their effects on insect detection and behavior. The study finds that florivores can detect more FVOCs than pollinators, and frequently tested FVOCs are often reported as attractive to pollinators and repellent to florivores.
Article
Microbiology
Paul Gaube, Polina Marchenko, Caroline Mueller, Rabea Schweiger, Raimund Tenhaken, Alexander Keller, Robert R. Junker
Summary: Microbes associated with flowers and leaves affect plant health and fitness and modify the chemical phenotypes of plants with consequences for interactions of plants with their environment. However, the drivers of bacterial communities colonizing above-ground parts of grassland plants in the field remain largely unknown.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Editorial Material
Plant Sciences
Rocky Putra, Caroline Mueller
Summary: Some plant species can tolerate and accumulate high levels of metals or metalloids in their tissues as a defense mechanism. These plants also synthesize specialized metabolites that act as organic defenses. The variation in the composition and concentration of these metabolites, known as chemodiversity, has received little attention in the elemental defense hypothesis.
Article
Plant Sciences
Rabea Schweiger, Alena-Maria Maidel, Tilo Renziehausen, Romy Schmidt-Schippers, Caroline Mueller
Summary: With changes in climate, the frequency of drought-waterlogging-redrying cycles will increase. This study investigated the effects of these cycles on wheat plants, focusing on biomass, photosynthesis, metabolites, and gene expression. The results showed that drought and waterlogging significantly reduced plant biomass. Transcript analysis revealed different gene expression patterns in response to these stressors. The findings provide insights into how wheat plants cope with changes in water regimes.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Plant Sciences
Dominik Ziaja, Caroline Mueller
Summary: Some plant species exhibit a high diversity in phytochemicals, which may contribute to their defense against herbivory. However, it is unclear whether individual plant's resistance to herbivores is determined by its own chemodiversity or by the chemodiversity of nearby plants. To investigate this, the researchers studied the role of chemodiversity in the interaction between Tanacetum vulgare plants and aphids.
FRONTIERS IN PLANT SCIENCE
(2023)
Correction
Ecology
Jeanne Friedrichs, Rabea Schweiger, Svenja Geisler, Judith M. Neumann, Sullivan J. M. Sadzik, Karsten Niehaus, Caroline Mueller
FRONTIERS IN ECOLOGY AND EVOLUTION
(2023)
Article
Behavioral Sciences
Pragya Singh, Jonas Wolthaus, Holger Schielzeth, Caroline Mueller
Summary: State variables, such as body condition, have an impact on behavioural traits. The effect of starvation on behaviour varies across different life stages. Starvation in larvae led to shorter postcontact immobility duration and higher activity levels, while starved adults were less active. Behavioural repeatability was found in both life stages, but starvation did not have a significant effect on the estimates.
Article
Biochemistry & Molecular Biology
Rocky Putra, Max Toelle, Ute Kraemer, Caroline Mueller
Summary: Soil pollution by metals and metalloids due to industrialization has a detrimental effect on ecosystems. Certain plant species called hyperaccumulators can accumulate high concentrations of these metals and metalloids in their aboveground tissues, acting as a defense against various antagonists. This study investigates the effects of metal(loid)s on defense traits in the hyperaccumulator plant species Arabidopsis halleri by artificially amending the soil with cadmium and zinc, and supplementing with silicon. The results show significant effects of metal amendment and silicon supplementation on foliar concentrations of certain elements, as well as on organic and mechanical defenses.
Article
Multidisciplinary Sciences
Thomas Dussarrat, Rabea Schweiger, Dominik Ziaja, Thuan T. N. Nguyen, Liv Krause, Ruth Jakobs, Elisabeth J. Eilers, Caroline Mueller
Summary: Intraspecific plant chemodiversity plays a crucial role in plant-environment interactions. Chemotypes can be defined based on the variation in dominant specialized metabolites. Maternal origin is found to be a higher source of chemical variation than chemotype. Computational models incorporating inherited variation in key terpenoids and other metabolites can help connect chemodiversity and evolutionary principles.
SCIENTIFIC REPORTS
(2023)
Article
Endocrinology & Metabolism
Silvia Eckert, Elisabeth J. Eilers, Ruth Jakobs, Redouan Adam Anaia, Kruthika Sen Aragam, Tanja Bloss, Moritz Popp, Rohit Sasidharan, Joerg-Peter Schnitzler, Florian Stein, Anke Steppuhn, Sybille B. Unsicker, Nicole M. van Dam, Sol Yepes, Dominik Ziaja, Caroline Mueller
Summary: In this study, the reproducibility of VOC collections and analyses was investigated in a ring trial involving five laboratories. The results showed that despite efforts to standardize each VOC measurement step, there were still differences in the outcomes both qualitatively and quantitatively. These findings reveal sources of variation in plant VOC research and can help avoid systematic errors in similar experiments.
Article
Agriculture, Multidisciplinary
Ann-Cathrin Voss, Elisabeth J. Eilers, Caroline Mueller
Summary: Numerous pesticides, including fungicides, are applied every year to crop plants, which may affect plant metabolism and thus crop quality. The present study tested the effects of two fungicides on the metabolism of two cultivars and the wild strawberry, focusing on primary and specialized metabolites that determine fruit flavor. The fungicide treatment significantly affected a number of metabolites, indicating potential changes in taste and quality of strawberries.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)