Article
Biochemistry & Molecular Biology
Tomas Aramburu, Joseph Kelich, Cory Rice, Emmanuel Skordalakes
Summary: Telomeric POT1-TPP1 binding is crucial for maintaining telomere integrity, and disrupting this complex can lead to cancer. This study demonstrates that the stability of POT1 is dependent on its binding with TPP1. The binding of TPP1 to POT1 stabilizes the POT1C domain and protects it from proteasomal degradation. Disruption of the POT1-TPP1 complex leads to reduced intracellular POT1 levels, telomere uncapping, and DNA damage response, which can ultimately result in cellular senescence, genomic instability, and oncogenesis.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Multidisciplinary Sciences
Melissa S. Traver, Bonnie Bartel
Summary: Lipid droplets are conserved organelles that regulate energy homeostasis by storing and releasing neutral lipids. This study identified MIEL1 as a protein that modulates lipid droplet dynamics by ubiquitinating and degrading the seed lipid droplet protein OLE1. The human homolog of MIEL1, PIRH2, was also found to be involved in lipid catabolism and peroxisome biology in mammals.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Shanshan Wang, Qian Hao, Jiajia Li, Yajie Chen, Hua Lu, Xiaohua Wu, Xiang Zhou
Summary: DTX3 plays a promoting role in ovarian cancer by enhancing the activity of mutant p53, and its expression is correlated with the expression of mutant p53 target genes and associated with unfavorable prognosis.
Article
Plant Sciences
Sujit Jung Karki, Aisling Reilly, Binbin Zhou, Maurizio Mascarello, James Burke, Fiona Doohan, Dimitar Douchkov, Patrick Schweizer, Angela Feechan
Summary: Septoria tritici blotch (STB) caused by Zymoseptoria tritici is a major threat to wheat production worldwide. The study identified a conserved cysteine-rich secreted effector, ZtSSP2, from Z. tritici, which interacts with wheat E3 ubiquitin ligase (TaE3UBQ) and may play a role in wheat immunity against the fungus. Further research is needed to understand the mechanisms of this interaction and potential implications for disease resistance in wheat.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Chemistry, Medicinal
Marina Minarro-Lleonar, Andrea Bertran-Mostazo, Jorge Duro, Xavier Barril, Jordi Juarez-Jimenez
Summary: Targeted protein degradation is a promising therapeutic strategy, and lenalidomide and pomalidomide are effective drugs in this field. These drugs stabilize the complex between CRBN and neo-substrates, leading to their degradation. The study identifies the role of hydrophobic shielding in stabilizing the CRBN-CK1 alpha complex and demonstrates the relationship between hydrogen bond robustness and binding affinities, providing insights for the design of improved protein degraders.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Orsolya Bilkei-Gorzo, Tiaan Heunis, Jose Luis Marin-Rubio, Francesca Romana Cianfanelli, Benjamin Bernard Armando Raymond, Joseph Inns, Daniela Fabrikova, Julien Peltier, Fiona Oakley, Ralf Schmid, Anetta Hartlova, Matthias Trost
Summary: This study reveals the importance of phagosomal ubiquitylation and the E3 ubiquitin ligase RNF115 in regulating innate immune functions during bacterial infections.
Article
Chemistry, Medicinal
Adam G. Bond, Conner Craigon, Kwok-Ho Chan, Andrea Testa, Athanasios Karapetsas, Rotimi Fasimoye, Thomas Macartney, J. Julian Blow, Dario R. Alessi, Alessio Ciulli
Summary: This study describes the design and development of a new protein degradation system utilizing a variant of the Brd4 bromodomain as a degradation tag. The system effectively degrades BromoTagged proteins in a fast, selective manner, showing favorable pharmacokinetic profile in mice. This system expands the arsenal of chemical genetic degradation tools for manipulating protein levels and exploring therapeutic potential in cells and in vivo.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Cell Biology
Martin P. Schwalm, Lena M. Berger, Maximilian N. Meuter, James D. Vasta, Cesear R. Corona, Sandra Roehm, Benedict-Tilman Berger, Frederic Farges, Sebastian M. Beinert, Franziska Preuss, Viktoria Morasch, Vladimir V. Rogov, Sebastian Mathea, Krishna Saxena, Matthew B. Robers, Susanne Mueller, Stefan Knapp
Summary: E3 ligases play a crucial role in regulating protein homeostasis by recruiting substrate proteins to the proteasomal degradation machinery. Recent research has focused on the Baculovirus IAP Repeat (BIR) family of E3 ligases, which contain a structurally conserved but diverse protein interaction domain. The Inhibitors of Apoptosis (IAP) family, which typically have three BIR domains, are promising drug targets. However, there is currently a lack of assay tools to evaluate the selectivity of inhibitors in this target area.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Rui Zhang, Shaoqing Shi
Summary: HECT-type E3 ubiquitin ligases play a vital role in controlling protein function and stability, and members of the NEDD4 family have critical roles in dysregulation of autophagy in cancer cells. This review focuses on the role of NEDD4 E3 ligases in defective autophagy in cancer cells, discussing their function, substrates, and signaling pathways, providing a basis for cancer treatment through modulation of these ligases.
MOLECULAR MEDICINE
(2023)
Article
Agricultural Engineering
Cui-Cui Yu, Pei-Wen Sun, Mei Rong, Zhi-hui Gao, Yang Liu, Meng-Jun Xiao, Jie-Mei Jiang, Yan-Hong Xu, Jian-He Wei
Summary: This study found that the synthesis of sesquiterpenes, active components in Agarwood, is regulated by C3HC4-type E3 ubiquitin ligase RING3, which promotes sesquiterpene biosynthesis by degrading transcription repressor AsWRKY44. This discovery provides a new perspective for exploring the regulation of transcriptional inhibition in other medicinal plants.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Review
Immunology
Haoran Cui, Yaxian Zhang, Leiliang Zhang
Summary: Poxviruses have evolved various mechanisms to evade innate immunity, some of which involve poxvirus-encoded E3 ubiquitin ligases and adaptor proteins. These proteins can be categorized into five groups based on their functional domains and ubiquitin transfer mechanisms. Most known substrates of poxvirus E3 ubiquitin ligases are components of the innate immune system. Current research progress provides mechanistic insights into the interaction between these viruses and their hosts.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kai Li, Yi Niu, Yichuan Yuan, Jiliang Qiu, Yunxing Shi, Chengrui Zhong, Zhiyu Qiu, Keren Li, Zhu Lin, Zhenkun Huang, Chao Zhang, Dinglan Zuo, Wei He, Yunfei Yuan, Binkui Li
Summary: Insufficient thermal ablation can promote the progression of hepatocellular carcinoma (HCC) and upregulate the E3 ligase Nedd4. Nedd4 enhances the TGF-beta signal transduction, promoting tumor growth and metastasis. High Nedd4 expression correlates with aggressive tumor phenotypes and poor prognosis in HCC patients.
Article
Plant Sciences
Jialing Zhang, Chaonan Li, Long Li, Yajun Xi, Jingyi Wang, Xinguo Mao, Ruilian Jing
Summary: TaAIRP2-1B regulates spike length by facilitating TaHIPP3 degradation, and the haplotype Hap-1B-1 of TaAIRP2-1B is a favorable natural variation for increasing spike length in wheat. This study provides insights into the role of E3 ubiquitin ligase genes in wheat development and identifies TaAIRP2-1B as a key regulator of spike length. The findings also offer genetic resources and markers for wheat molecular breeding.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Review
Biochemistry & Molecular Biology
Ishita Tripathi-Giesgen, Christian Behrends, Arno F. Alpi
Summary: The ubiquitin system plays a crucial role in the host cellular defense program against bacterial infection, especially when certain bacteria are exposed to the host cytosol during invasion. Host cell E3 ubiquitin ligases contribute to the formation of a protective ubiquitin coat on invading pathogens, with their divergent ubiquitin conjugation mechanisms influencing the complexity of the anti-bacterial coating. Bacteria have evolved strategies to evade the activities of the host ubiquitin system.
Review
Biochemistry & Molecular Biology
Dong Wang, Yuanming Zou, Xinyue Huang, Zeyu Yin, Mohan Li, Jiaqi Xu, Boquan Wu, Da Li, Ying Zhang, Yingxian Sun, Xingang Zhang, Naijin Zhang
Summary: The ubiquitin-proteasome system is crucial for regulating protein levels in cells, and SMURF1 and SMURF2 are important components that maintain physiological processes by regulating the stability of multiple proteins. The regulatory functions of SMURFs in disease progression are complex, either facilitative or inhibitory, and understanding their mechanisms offers potential therapeutic targets and new avenues for research.
Article
Polymer Science
Guanjun Ding, Ao Wang, Xinming Shi, Jumei Li, Lijun You, Shaoyun Wang
Summary: Polyphosphazene microspheres were encoded with fluorescence and surface enhanced Raman spectra, serving as solid phase carriers for antibody detection. The prepared multispectral encoded microspheres showed stable and distinguishable encoding signals, large encoding volume, and multiplexed analytical capability in sandwich immunoassays.
Article
Chemistry, Physical
Jiafeng He, Gao Song, Xinyue Wang, Ling Zhou, Jumei Li
Summary: The study developed magnetic Fe3O4/GO/Ag composite microspheres for removing organic dyes and antibiotics in water, showing high activity in SERS detection and catalytic reactions. The microspheres exhibited potential applications in trace and quantitative detection of chemicals, as well as in-situ monitoring of the catalytic degradation of organic pollutants.
JOURNAL OF ALLOYS AND COMPOUNDS
(2022)
Article
Materials Science, Multidisciplinary
Yuanzhi Min, Gao Song, Ling Zhou, Xinyue Wang, Pingying Liu, Jumei Li
Summary: This study presents a facile approach for fabricating silver@mesoporous anatase TiO(2) nanoparticles and investigates their applications in SERS analysis and dye pollutant removal. The nanoparticles exhibit excellent sensitivity and catalytic activity, along with good stability and recyclability.
Article
Engineering, Multidisciplinary
Xinyue Wang, Huan Cheng, Yuanzhi Min, Xian Li, Lijun You, Jumei Li
Summary: A novel magnetic Fe3O4/m-ZrO2/Ag nanocomposite with excellent SERS activity and photocatalytic property was developed, in which a crystalline mesoporous ZrO2 interlayer was introduced. The SERS sensitivity of Fe3O4/m-ZrO2/Ag nanocomposites was controlled by adjusting the coverage of Ag nanoparticles. These nanocomposites exhibited remarkable SERS sensitivity, exceptional signal reproducibility, and excellent storing stability, capable of detecting R6G and Cr (VI) at low concentrations. Moreover, the nanocomposites achieved recyclable detection of R6G and Cr (VI) through photocatalytic removal of pollutants under UV irradiation. Overall, the prepared Fe3O4/m-ZrO2/Ag nanocomposites have high SERS sensitivity and good photocatalytic performance, which are of great significance for environmental detection and ecological restoration.
COMPOSITES PART B-ENGINEERING
(2022)
Article
Engineering, Environmental
Linheng Chen, Weixi Liu, Yang Wu, Jumei Li, Yibing Ma
Summary: This study investigated the effects of Cr(III) and Cr(VI) on barley root elongation. The results showed weak antagonism effects of Cr(III) and Cr(VI) on barley root toxicity. By introducing the expansion coefficient of Cr(III) on Cr(VI) toxicity, the barley root elongation under their interaction could be more accurately predicted.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Chemistry, Physical
Xian Li, Pingying Liu, Jumei Li
Summary: In this study, a facile and efficient approach was developed to fabricate magnetically separable core-shell structured nanocomposites. The Fe3O4/mZrO(2)/Ag nanocomposites exhibited higher photocatalytic activity compared to Fe3O4/mZrO(2) composites, and showed remarkable reusability due to their easy separation and recycling with an external magnetic field.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Engineering, Chemical
Babar Hussain, Yibing Ma, Jumei Li, Jusheng Gao, Aman Ullah, Nazia Tahir
Summary: The long-term experiment showed that Cl- based fertilizers significantly increased the accumulation of Cd in rice plants, while SO42- had no significant effect. However, the short-term experiment revealed that SO42- based fertilizers reduced the uptake of Cd by rice plants from Cd contaminated soils. These findings are important for rational fertilization and safer rice production.
Article
Chemistry, Physical
Xian Li, Jiafeng He, Weilong Zhang, Lijun You, Jumei Li
Summary: A sandwich structured magnetic nanosphere with Fe3O4/Ag/m(TiO2-ZrO2) was fabricated, which utilized in situ deposition of Ag nanoparticles onto Fe3O4 nanospheres and subsequent coating of mesoporous TiO2-ZrO2. The sandwich nanospheres showed higher efficient charge migration and larger specific surface area, leading to improved photocatalytic activity for Cr(VI) reduction. Furthermore, the nanospheres exhibited high thermal stability due to the protection of the interim Ag nanoparticles by the mesoporous TiO2-ZrO2 shell. This work provided a good example for the design and preparation of high-performance magnetic photocatalysts for the remediation of heavy metal pollutants.
APPLIED SURFACE SCIENCE
(2023)
Correction
Chemistry, Physical
Jie Yang, Gao Song, Ling Zhou, Xinyue Wang, Lijun You, Jumei Li
APPLIED SURFACE SCIENCE
(2023)
Article
Chemistry, Physical
Ling Zhou, Xiaojun Wen, Yuanzhi Min, Jiafeng He, Lijun You, Jumei Li
Summary: In this study, a novel SERS platform with high sensitivity and photocatalytic performance was successfully constructed for in situ monitoring of chemical reactions and photocatalytic reduction processes. The experimental results demonstrated a low detection limit for Cr(VI) and a good linear relationship. Moreover, the platform exhibited excellent signal reproducibility and storage stability.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Infectious Diseases
Huilin Ren, Jumei Li, Xiao Xie, Min Xu, Yuhua Yang, Xie Gao
Summary: This study constructed a prediction model based on the clinical characteristics of epidermoid cysts to identify pathologic infections. The model showed good diagnostic accuracy and high specificity.
SURGICAL INFECTIONS
(2022)
Article
Agronomy
Jin Liu, Chaoqun Han, Yuhang Zhao, Dongling Yang, Jianjun Yang, Lei Zheng, Yongfeng Hu, Jumei Li, Peng Sui, Yuanquan Chen, Xiaojun Shi, Yibing Ma
Summary: This study investigated the molecular transformation mechanisms of phosphorus in Entisol soils under different long-term fertilization regimes. The results showed that PK and NPKM treatments resulted in higher phosphorus availability in the soils, which can benefit the improvement of fertilization strategies in agricultural soils.
Article
Chemistry, Physical
Lijun You, Baohua Zhong, Ci Huang, Jumei Li, Zhijuan Zheng, Yang Wang
Summary: Au coated magnetic polyphosphazene (MPCTP) composite particles (MPCTP@Au) with sensitive SERS activity were successfully fabricated. MPCTP particles were generated by coating polyphosphazene on Fe3O4 nanoparticles. MPCTP@Au composite particles were obtained by deposition of Au nanoparticles on MPCTP. The magnetic core endowed the composite particles with good magnetic responsiveness, simplifying the sample pretreatment procedure.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Chemistry, Analytical
Huan Cheng, Xiaojun Wen, Kuang Luo, Lijun You, Jumei Li
Summary: This study successfully synthesized Cu2O/Ag nanocomposites with photocatalytic and SERS properties, which showed high sensitivity and reusability in detecting colorant additives in fruit juice. The nanocomposites exhibited exceptional detection limits and strong linear relationships between SERS intensities and concentrations. Moreover, the Cu2O/Ag nanocomposites demonstrated practical recyclability and efficient dye degradation under UV light irradiation.
MICROCHEMICAL JOURNAL
(2023)
Article
Engineering, Environmental
Jiafeng He, Xian Li, Jumei Li
Summary: This study demonstrates a reliable surface-enhanced Raman scattering (SERS) substrate composed of graphene oxide (GO) nanosheets and silver nanocubes for sensitive detection of various organic contaminants. The SERS substrate shows highly uniform signals and low relative standard deviations, and is capable of label-free detection of complex organic pesticides.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)