Article
Chemistry, Analytical
Juan Song, Shan Li, Jie Zhou, Qiao Yu, Xue-Jiao Yang, Hong-Yuan Chen, Jing-Juan Xu
Summary: We have developed a triple signal amplification strategy for in situ visualization of telomerase RNA (TR) in living cells. The strategy includes target-initiated strand displacement circuit, multidirectional rolling circle amplification (RCA), and Mg2+ DNAzyme-mediated amplification. The proposed method exhibited ultrasensitive detection of TR mimic with a detection limit at the picomolar level. Moreover, after transfection with the proposed sensing platform, tumor cells can be easily distinguished from normal cells based on TR abundance-related fluorescence signal, providing new insights into initial cancer screening.
ANALYTICA CHIMICA ACTA
(2023)
Review
Biochemistry & Molecular Biology
Nikita Shepelev, Olga Dontsova, Maria Rubtsova
Summary: Telomere length is associated with cell proliferative potential and is maintained by telomerase. Telomerase is activated during cellular division and plays a crucial role in processes like regeneration, immune response, embryonic development, and cancer progression. Understanding the regulatory mechanisms of telomerase biogenesis and activity is important for manipulating telomerase and influencing these processes. This review focuses on the molecular mechanisms involved in telomerase regulation and the role of post-transcriptional and post-translational modifications in telomerase biogenesis and function in yeast and vertebrates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Wasif Al-Shareef, Yogev Brown, Christopher Bryan, Elena Shuvaeva, Shhadeh Bsoul, Raanan Greenman, Majdi M. Kabaha, Nikolai B. Ulyanov, Emmanuel Skordalakes, Yehuda Tzfati
Summary: Telomerase is composed of telomerase RNA and telomerase reverse transcriptase, which maintain chromosome end integrity by copying a template onto chromosome ends. Despite diversity in telomerase RNA sequence across fungi and mammals, structural motifs essential for telomerase function are conserved. Est2 interacts with specific RNA structures and mutations in these domains cause severe telomere shortening, highlighting the conservation and importance of these interactions for telomerase function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Chemistry, Applied
Xiaoyu Guo, Ji Kang, Zhiyuan Xu, Qingbin Guo, Lifeng Zhang, Haifeng Ning, Steve W. Cui
Summary: This review specifically examines the formation mechanisms, structure-activity relationships, and analytical methods for triple-helix polysaccharides. The insights provided can be valuable for exploring the biological activities of polysaccharides and their applications in the food and pharmaceutical industries.
CARBOHYDRATE POLYMERS
(2021)
Article
Biochemistry & Molecular Biology
Charlotte N. Kunkler, Grace E. Schiefelbein, Nathan J. O'Leary, Phillip J. Mccown, Jessica A. Brown
Summary: Recent studies suggest that noncoding RNAs can interact with genomic DNA to form RNA center dot DNA-DNA triple helices, which play a role in regulating transcription. Researchers have found that RNA modifications may influence the formation and stability of these triple helices.
Review
Biochemistry & Molecular Biology
Rodrigo Maldonado, Gernot Laengst
Summary: Mammalian genomes are transcribed extensively, producing numerous coding and non-coding transcripts. These transcripts are physically associated with chromatin and play roles in gene regulation and genome organization. This review focuses on the sequence-specific targeting of RNA to DNA by forming triple helical structures and discusses the importance of nucleosome positioning and histone modifications in stabilizing these structures.
BIOLOGICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Jesus Garcia-Castillo, Francisca Alcaraz-Perez, Elena Martinez-Balsalobre, Diana Garcia-Moreno, Marlies P. Rossmann, Miriam Fernandez-Lajarin, Manuel Bernabe-Garcia, Ana B. Perez-Oliva, Virginia C. Rodriguez-Cortez, Clara Bueno, Isaac Adatto, Suneet Agarwal, Pablo Menendez, Leonard Zon, Victoriano Mulero, Maria L. Cayuela
Summary: Dyskeratosis congenita (DC) is a rare genetic syndrome caused by mutations in telomerase or telomeric proteins. Research shows that zebrafish terc plays a crucial role in regulating the expression of master myeloid genes, while mutations found in DC patients affect this process.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Oncology
Guangshi Du, Jian Sun, Zhen Li, Qian Zhang, Wenjing Liu, Chuanyu Yang, Ping Zhao, Xinye Wang, Qiyan Yin, Yao Luo, Jinhuan Song, Yi Wen, Haixia Wang, Chuan-Huizi Chen, Guosheng Hu, Zhongmei Zhou, Xiaoyun Mao, Wen Liu, Zhenzhen Liu, Dewei Jiang, Ceshi Chen
Summary: This study identifies the promoting role of KPRT4 in BLBC cell proliferation and reveals the underlying mechanism of mutual regulation between KLF5 and KPRT4. Additionally, the study finds that targeting KPRT4 can attenuate tumor growth, suggesting KPRT4 as a major molecule for BLBC progression.
Article
Chemistry, Applied
Xiaolong Yang, Siqing Wei, Xiaoming Lu, Xuguang Qiao, Jesus Simal-Gandara, Esra Capanoglu, Lukasz Wozniak, Liang Zou, Hui Cao, Jianbo Xiao, Xiaozhen Tang, Ningyang Li
Summary: A neutral ginger polysaccharide fraction (NGP) isolated from ginger was found to have a low molecular weight primarily consisting of glucose, with immunomodulatory activity on RAW264.7 cells. NGP could enhance macrophage proliferation, increase immune substance production, and potentially serve as an immune agent.
Article
Multidisciplinary Sciences
Haina Huang, Katrin Karbstein
Summary: This passage discusses the tendency of three-helix junctions to misfold during the assembly of small ribosomal subunits in cells, and reveals the pivotal role of ribosome assembly factors in facilitating their correct folding.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Stefano Gnan, Melody Matelot, Marion Weiman, Olivier Arnaiz, Frederic Guerin, Linda Sperling, Mireille Betermier, Claude Thermes, Chun-Long Chen, Sandra Duharcourt
Summary: This study reveals the complexity of intron recognition in Paramecium tetraurelia, and finds that GC content and nucleosome positioning have distinct effects on intron splicing efficiency.
Article
Genetics & Heredity
Gabriella Assante, Sriram Chandrasekaran, Stanley Ng, Aikaterini Tourna, Carolina H. Chung, Kowsar A. Isse, Jasmine L. Banks, Ugo Soffientini, Celine Filippi, Anil Dhawan, Mo Liu, Steven G. Rozen, Matthew Hoare, Peter Campbell, J. William O. Ballard, Nigel Turner, Margaret J. Morris, Shilpa Chokshi, Neil A. Youngson
Summary: The research demonstrates that metabolically driven histone hyperacetylation in steatotic hepatocytes can increase DNA damage, thus initiating the development of HCC.
Article
Chemistry, Multidisciplinary
Tomas Fiala, Emilia P. Barros, Marc-Olivier Ebert, Enrico Ruijsenaars, Sereina Riniker, Helma Wennemers
Summary: This study reveals the significant impact of terminal residues on the stability of collagen model peptides (CMPs). By combining various experimental and simulation techniques, the researchers demonstrate how peptide termini preorganize into a polyproline-II helical structure and affect the stability of triple helices. These findings have important implications for designing peptide mimics of structural proteins and provide a straightforward method to tune the stability of CMPs for various applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Qi Zhu, Simin Yao, Ziying Ye, Peipei Jiang, Huiyan Wang, Xiwen Zhang, Dan Liu, Haining Lv, Chenrui Cao, Zhenhua Zhou, Zihan Zhou, Weichen Pan, Guangfeng Zhao, Yali Hu
Summary: This study found that there is an increase in ferroptosis load in the endometria of patients with intrauterine adhesions (IUA). Ferroptosis promotes epithelial-mesenchymal transition (EMT) and fibrosis in endometrial epithelial cells, and its inhibition can ameliorate endometrial fibrosis in a murine model of IUA. These findings suggest that ferroptosis may serve as a potential therapeutic target for endometrial fibrosis in IUA.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Engineering, Industrial
Steffan James, Zheng Liu, Gareth R. T. White, Anthony Samuel
Summary: Triple Helix has been widely discussed as a means of enabling innovation and economic development. This study examines its functioning under crisis conditions and the ethical relationships between its actors. The findings show that the interoperation of Triple Helix is durable under crisis conditions, and micro-relations between actors enable ideation of new devices and minimize bureaucratic obstacles through supererogatory acts.