Unravelling the structural and mechanistic basis of CRISPR–Cas systems

Requirements for a successful defence reaction by the CRISPR–Cas subtype I-B system

(2015) Britta Stoll et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

The basic building blocks and evolution of CRISPR–Cas systems

(2015) Kira S. Makarova et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

(2014) Hiroshi Nishimasu et al.   CELL

Fitting CRISPR-associated Cas3 into the Helicase Family Tree

(2014) Ryan N. Jackson et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

(2014) Samuel H. Sternberg et al.   NATURE

In vitro assembly and activity of an archaeal CRISPR-Cas type I-A Cascade interference complex

(2014) André Plagens et al.   NUCLEIC ACIDS RESEARCH

CasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference

(2014) M. L. Hochstrasser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Degenerate target sites mediate rapid primed CRISPR adaptation

(2014) P. C. Fineran et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation

(2014) M. Jinek et al.   SCIENCE

Planting the seed: target recognition of short guide RNAs

(2014) Tim Künne et al.   TRENDS IN MICROBIOLOGY

Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems

(2014) A. A. Gomaa et al.   mBio

CRISPR-Mediated Adaptive Immune Systems in Bacteria and Archaea

(2013) Rotem Sorek et al.   Annual Review of Biochemistry

Structure of a dimeric crenarchaeal Cas6 enzyme with an atypical active site for CRISPR RNA processing

(2013) Judith Reeks et al.   BIOCHEMICAL JOURNAL

CRISPR interference: a structural perspective

(2013) Judith Reeks et al.   BIOCHEMICAL JOURNAL

Exploiting CRISPR/Cas systems for biotechnology

(2013) Timothy R. Sampson et al.   BIOESSAYS

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus

(2013) Tomas Sinkunas et al.   EMBO JOURNAL

Genetic Characterization of Antiplasmid Immunity through a Type III-A CRISPR-Cas System

(2013) A. Hatoum-Aslan et al.   JOURNAL OF BACTERIOLOGY

A Ruler Protein in a Complex for Antiviral Defense Determines the Length of Small Interfering CRISPR RNAs

(2013) Asma Hatoum-Aslan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Crystal Structure of the Cmr2–Cmr3 Subcomplex in the CRISPR–Cas RNA Silencing Effector Complex

(2013) Takuo Osawa et al.   JOURNAL OF MOLECULAR BIOLOGY

Conservation and Variability in the Structure and Function of the Cas5d Endoribonuclease in the CRISPR-Mediated Microbial Immune System

(2013) Yoon Koo et al.   JOURNAL OF MOLECULAR BIOLOGY

Toroidal Structure and DNA Cleavage by the CRISPR-Associated [4Fe-4S] Cluster Containing Cas4 Nuclease SSO0001 from Sulfolobus solfataricus

(2013) Sofia Lemak et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Structure of an RNA Silencing Complex of the CRISPR-Cas Immune System

(2013) Michael Spilman et al.   MOLECULAR CELL

Structure and Activity of the RNA-Targeting Type III-B CRISPR-Cas Complex of Thermus thermophilus

(2013) Raymond H.J. Staals et al.   MOLECULAR CELL

Structure of the CRISPR Interference Complex CSM Reveals Key Similarities with Cascade

(2013) Christophe Rouillon et al.   MOLECULAR CELL

Same Same but Different: New Structural Insight into CRISPR-Cas Complexes

(2013) Nadja Heidrich et al.   MOLECULAR CELL

A novel interference mechanism by a type IIIB CRISPR-Cmr module inSulfolobus

(2013) Ling Deng et al.   MOLECULAR MICROBIOLOGY

A bacteriophage encodes its own CRISPR/Cas adaptive response to evade host innate immunity

(2013) Kimberley D. Seed et al.   NATURE

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Revenge of the phages: defeating bacterial defences

(2013) Julie E. Samson et al.   NATURE REVIEWS MICROBIOLOGY

Evolution of CRISPR RNA recognition and processing by Cas6 endonucleases

(2013) O. Niewoehner et al.   NUCLEIC ACIDS RESEARCH

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

(2013) David Bikard et al.   NUCLEIC ACIDS RESEARCH

Comparative genomics of defense systems in archaea and bacteria

(2013) Kira S. Makarova et al.   NUCLEIC ACIDS RESEARCH

Double-strand DNA end-binding and sliding of the toroidal CRISPR-associated protein Csn2

(2013) Zihni Arslan et al.   NUCLEIC ACIDS RESEARCH

Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems

(2013) Ines Fonfara et al.   NUCLEIC ACIDS RESEARCH

Structure-based cleavage mechanism of Thermus thermophilus Argonaute DNA guide strand-mediated DNA target cleavage

(2013) G. Sheng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rapid evolution of the human gut virome

(2013) S. Minot et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Identification, structural, and biochemical characterization of a group of large Csn2 proteins involved in CRISPR-mediated bacterial immunity

(2013) Kwang-Hoon Lee et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Structure and RNA-binding properties of the Type III-A CRISPR-associated protein Csm3

(2013) Ajla Hrle et al.   RNA Biology

Protospacer recognition motifs

(2013) Shiraz A. Shah et al.   RNA Biology

CRISPR-spacer integration reporter plasmids reveal distinct genuine acquisition specificities among CRISPR-Cas I-E variants ofEscherichia coli

(2013) César Díez-Villaseñor et al.   RNA Biology

The CRISPR Craze

(2013) E. Pennisi   SCIENCE

Structure of the Cmr2-Cmr3 Subcomplex of the Cmr RNA Silencing Complex

(2013) Yaming Shao et al.   STRUCTURE

CRISPR-Cas and restriction–modification systems are compatible and increase phage resistance

(2013) Marie-Ève Dupuis et al.   Nature Communications

Differential Translation Tunes Uneven Production of Operon-Encoded Proteins

(2013) Tessa E.F. Quax et al.   Cell Reports

Cytotoxic Chromosomal Targeting by CRISPR/Cas Systems Can Reshape Bacterial Genomes and Expel or Remodel Pathogenicity Islands

(2013) Reuben B. Vercoe et al.   PLoS Genetics

Type I-E CRISPR-Cas Systems Discriminate Target from Non-Target DNA through Base Pairing-Independent PAM Recognition

(2013) Edze R. Westra et al.   PLoS Genetics

The CRISPRs, They Are A-Changin': How Prokaryotes Generate Adaptive Immunity

(2012) Edze R. Westra et al.   Annual Review of Genetics

Csy4 relies on an unusual catalytic dyad to position and cleave CRISPR RNA

(2012) Rachel E Haurwitz et al.   EMBO JOURNAL

Crystal structure of Cmr2 suggests a nucleotide cyclase-related enzyme in type III CRISPR-Cas systems

(2012) Xing Zhu et al.   FEBS LETTERS

Characterization of the CRISPR/Cas Subtype I-A System of the Hyperthermophilic Crenarchaeon Thermoproteus tenax

(2012) A. Plagens et al.   JOURNAL OF BACTERIOLOGY

Crystal Structure of the Largest Subunit of a Bacterial RNA-guided Immune Complex and Its Role in DNA Target Binding

(2012) Sabin Mulepati et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Double-stranded Endonuclease Activity inBacillus haloduransClustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated Cas2 Protein

(2012) Ki Hyun Nam et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The crystal structure of the CRISPR-associated protein Csn2 from Streptococcus agalactiae

(2012) Philipp Ellinger et al.   JOURNAL OF STRUCTURAL BIOLOGY

Native Tandem and Ion Mobility Mass Spectrometry Highlight Structural and Modular Similarities in Clustered-Regularly-Interspaced Shot-Palindromic-Repeats (CRISPR)-associated Protein Complexes FromEscherichia coliandPseudomonas aeruginosa

(2012) Esther van Duijn et al.   MOLECULAR & CELLULAR PROTEOMICS

Structure and Mechanism of the CMR Complex for CRISPR-Mediated Antiviral Immunity

(2012) Jing Zhang et al.   MOLECULAR CELL

Essential Features and Rational Design of CRISPR RNAs that Function with the Cas RAMP Module Complex to Cleave RNAs

(2012) Caryn R. Hale et al.   MOLECULAR CELL

CRISPR Immunity Relies on the Consecutive Binding and Degradation of Negatively Supercoiled Invader DNA by Cascade and Cas3

(2012) Edze R. Westra et al.   MOLECULAR CELL

Mechanism of Foreign DNA Selection in a Bacterial Adaptive Immune System

(2012) Dipali G. Sashital et al.   MOLECULAR CELL

Selective and hyperactive uptake of foreign DNA by adaptive immune systems of an archaeon via two distinct mechanisms

(2012) Susanne Erdmann et al.   MOLECULAR MICROBIOLOGY

RNA-guided genetic silencing systems in bacteria and archaea

(2012) Blake Wiedenheft et al.   NATURE

Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli

(2012) Ido Yosef et al.   NUCLEIC ACIDS RESEARCH

Crystal Structure of Streptococcus pyogenes Csn2 Reveals Calcium-Dependent Conformational Changes in Its Tertiary and Quaternary Structure

(2012) Yoon Koo et al.   PLoS One

CRISPR Interference Directs Strand Specific Spacer Acquisition

(2012) Daan C. Swarts et al.   PLoS One

Cleavage of Phage DNA by the Streptococcus thermophilus CRISPR3-Cas System

(2012) Alfonso H. Magadán et al.   PLoS One

In Vivo Protein Interactions and Complex Formation in the Pectobacterium atrosepticum Subtype I-F CRISPR/Cas System

(2012) Corinna Richter et al.   PLoS One

Target Motifs Affecting Natural Immunity by a Constitutive CRISPR-Cas System in Escherichia coli

(2012) Cristóbal Almendros et al.   PLoS One

The CRISPR Associated Protein Cas4 Is a 5′ to 3′ DNA Exonuclease with an Iron-Sulfur Cluster

(2012) Jing Zhang et al.   PLoS One

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cascade-mediated binding and bending of negatively supercoiled DNA

(2012) Edze R. Westra et al.   RNA Biology

Mechanism of substrate selection by a highly specific CRISPR endoribonuclease

(2012) S. H. Sternberg et al.   RNA

Cas5d processes pre-crRNA and is a member of a larger family of CRISPR RNA endonucleases

(2012) E. L. Garside et al.   RNA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Structure of the Cmr2 Subunit of the CRISPR-Cas RNA Silencing Complex

(2012) Alexis I. Cocozaki et al.   STRUCTURE

Cas5d Protein Processes Pre-crRNA and Assembles into a Cascade-like Interference Complex in Subtype I-C/Dvulg CRISPR-Cas System

(2012) Ki Hyun Nam et al.   STRUCTURE

Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system

(2012) Kirill A. Datsenko et al.   Nature Communications

Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systems

(2011) Kira S Makarova et al.   Biology Direct

Structure and activity of the Cas3 HD nuclease MJ0384, an effector enzyme of the CRISPR interference

(2011) Natalia Beloglazova et al.   EMBO JOURNAL

Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system

(2011) Tomas Sinkunas et al.   EMBO JOURNAL

The human gut virome: Inter-individual variation and dynamic response to diet

(2011) S. Minot et al.   GENOME RESEARCH

Crystal Structure of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated Csn2 Protein Revealed Ca2+-dependent Double-stranded DNA Binding Activity

(2011) Ki Hyun Nam et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural and Biochemical Analysis of Nuclease Domain of Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated Protein 3 (Cas3)

(2011) Sabin Mulepati et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural and Functional Characterization of an Archaeal Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated Complex for Antiviral Defense (CASCADE)

(2011) Nathanael G. Lintner et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

Structures of the RNA-guided surveillance complex from a bacterial immune system

(2011) Blake Wiedenheft et al.   NATURE

Evolution and classification of the CRISPR–Cas systems

(2011) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Recognition and maturation of effector RNAs in a CRISPR interference pathway

(2011) Emily M Gesner et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

An RNA-induced conformational change required for CRISPR RNA cleavage by the endoribonuclease Cse3

(2011) Dipali G Sashital et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural basis for CRISPR RNA-guided DNA recognition by Cascade

(2011) Matthijs M Jore et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Mature clustered, regularly interspaced, short palindromic repeats RNA (crRNA) length is measured by a ruler mechanism anchored at the precursor processing site

(2011) A. Hatoum-Aslan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions

(2011) B. Wiedenheft et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence

(2011) E. Semenova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Csy4 is responsible for CRISPR RNA processing inPectobacterium atrosepticum

(2011) Rita Przybilski et al.   RNA Biology

Interaction of the Cas6 Riboendonuclease with CRISPR RNAs: Recognition and Cleavage

(2011) Ruiying Wang et al.   STRUCTURE

Structure of a CRISPR-associated protein Cas2 fromDesulfovibrio vulgaris

(2010) Poulami Samai et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

A dual function of the CRISPR-Cas system in bacterial antivirus immunity and DNA repair

(2010) Mohan Babu et al.   MOLECULAR MICROBIOLOGY

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

(2010) Josiane E. Garneau et al.   NATURE

Self versus non-self discrimination during CRISPR RNA-directed immunity

(2010) Luciano A. Marraffini et al.   NATURE

Binding and cleavage of CRISPR RNA by Cas6

(2010) J. Carte et al.   RNA

Sequence- and Structure-Specific RNA Processing by a CRISPR Endonuclease

(2010) Rachel E. Haurwitz et al.   SCIENCE

Self-targeting by CRISPR: gene regulation or autoimmunity?

(2010) Adi Stern et al.   TRENDS IN GENETICS

RNA-Guided RNA Cleavage by a CRISPR RNA-Cas Protein Complex

(2009) Caryn R. Hale et al.   CELL

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

(2009)   MICROBIOLOGY-SGM

CRISPR families of the crenarchaeal genus Sulfolobus: bidirectional transcription and dynamic properties

(2009) Reidun K. Lillestøl et al.   MOLECULAR MICROBIOLOGY

Structural Basis for DNase Activity of a Conserved Protein Implicated in CRISPR-Mediated Genome Defense

(2009) Blake Wiedenheft et al.   STRUCTURE

CRISPR-based adaptive and heritable immunity in prokaryotes

(2009) John van der Oost et al.   TRENDS IN BIOCHEMICAL SCIENCES

Cas6 is an endoribonuclease that generates guide RNAs for invader defense in prokaryotes

(2008) J. Carte et al.   GENES & DEVELOPMENT

A Novel Family of Sequence-specific Endoribonucleases Associated with the Clustered Regularly Interspaced Short Palindromic Repeats

(2008) Natalia Beloglazova et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE

CRISPR Interference Limits Horizontal Gene Transfer in Staphylococci by Targeting DNA

(2008) Luciano A. Marraffini et al.   SCIENCE