Article
Multidisciplinary Sciences
Jung Yeon Han, Joseph N. La Fiandra, Don L. DeVoe
Summary: This paper presents a microfluidic vortex focusing technique for mass production of liposomes with controlled size and low variability, addressing the trade-offs between size uniformity and mass production in liposome synthesis.
NATURE COMMUNICATIONS
(2022)
Review
Chemistry, Analytical
Sheng Yan, Dan Yuan
Summary: The development of microfluidic technologies, particularly microflow cytometry with 3D focusing capabilities, has shown great promise in single-cell analysis, offering a wide range of applications in biology and medicine.
Article
Polymer Science
Guk-Young Ahn, Inseong Choi, Minju Song, Soo Kyung Han, Kangho Choi, Young-Hyun Ryu, Do-Hyun Oh, Hye-Won Kang, Sung-Wook Choi
Summary: In this study, a polydimethylsiloxane (PDMS) microfluidic chip with well-interconnected microfibrous channels was fabricated using electrospun poly(e-caprolactone) (PCL) microfibrous matrix and 3D-printed patterns as templates. The chip, known as microfiber-templated microfluidic chip (MTMC), demonstrated high throughput and continuous production of nanoscale emulsions and spheres by utilizing mechanisms such as cross-junction dripping and Y-junction splitting. The MTMC allows for controlled size ranges of water-in-oil emulsions and polyethylene glycol-diacrylate (PEG-DA) spheres, and can potentially produce 58 trillion PEG-DA nanospheres per hour without high shear force. Additionally, the MTMC showed higher encapsulation efficiency compared to microspheres fabricated by ultrasonication, making it a powerful platform for large-scale and continuous production of emulsions and spheres.
Article
Chemistry, Multidisciplinary
Yazhi Zheng, Hanxu Chen, Xiang Lin, Minli Li, Yuanjin Zhao, Luoran Shang
Summary: Drug microcarriers were prepared using a microfluidic step emulsification method, which allows for high-throughput production of droplets and hydrogel microparticles. The microparticles encapsulated basic fibroblast growth factor (bFGF) and incorporated black phosphorus (BP) for controllable release via near-infrared (NIR) stimulation. These microparticles serve as drug carriers to induce angiogenesis and collagen deposition, promoting wound repair. These findings suggest that the step emulsification technique has the potential to provide scalable production of drug microcarriers for wound healing and tissue regeneration.
Article
Chemistry, Multidisciplinary
Marta Napiorkowska, Luzius Pestalozzi, Sven Panke, Martin Held, Steven Schmitt
Summary: A high-throughput platform for optimizing microbial protein production is described, using droplet microfluidics and flow cytometry. The platform successfully cultures several industrially relevant bacterial and yeast strains and isolates strains with superior production titers by detecting peptides or proteins released from the cell via autolysis or secretion.
Article
Nanoscience & Nanotechnology
Wenjun Ma, Dong Liu, Sida Ling, Jingwei Zhang, Zhuo Chen, Yuan Lu, Jianhong Xu
Summary: By improving the structure of microfluidic devices, high-throughput fabrication of helical microfibers has been achieved, solving the previous issues of channel blockage and spinning instability, and providing more possibilities for the application of helical microfibers.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Bo Zhang, Ansila Mathoor, Tanja Junkers
Summary: Describes an automated high-throughput multidimensional reaction screening platform based on an inline Fourier-transform infrared spectroscopy. The platform combines flow chemistry, machine automation, and inline analysis to rapidly and efficiently screen reactions in multiple dimensions. It enables the construction of kinetic data libraries with high precision, reproducibility, and data density. The method allows for precise prediction of reaction outcomes within the high-dimensional chemical parameter space that is screened, and is a key step in providing big data for data-driven research.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Physical
Fazlollah Heshmatnezhad, Ali Reza Solaimany Nazar, Halimeh Aghaei, Jaleh Varshosaz
Summary: This study demonstrated a simple method for producing DOX-loaded PCL nanoparticles with uniform size distribution and high EE through a microfluidic device. The release analysis showed sustained release of the nanoparticles for 10 days at pH 7.4, indicating good sustained-release performance.
Article
Biochemical Research Methods
June Soo Kim, Soon Yeol Kwon, Jae Yong Lee, Seung Deok Kim, Da Ye Kim, Hyunjun Kim, Noah Jang, Jiajie Wang, Maeum Han, Seong Ho Kong
Summary: A microfluidic resistive pulse sensing technique is presented for detecting and analysing microparticles, with challenges in noise and low throughput. The study introduces a microfluidic chip with multiple detection gates to improve throughput while maintaining simplicity. The proposed chip uses hydrodynamic sheathless particle focusing to detect resistive pulses, with a reference gate to minimize noise. It achieves high sensitivity in analysing physical properties of particles and exosomes, offering potential for exosome detection in biological and clinical applications.
Article
Multidisciplinary Sciences
Tharagan Kumar, Harisha Ramachandraiah, Sharath Narayana Iyengar, Indradumna Banerjee, Gustaf Martensson, Aman Russom
Summary: In this study, high Reynolds number viscoelastic particle focusing and separation in spiral channels was explored. The balance between dominant inertial lift force, dean drag force, and elastic force enables stable 3D particle focusing at dynamically high Reynolds numbers. Effective separation based on size was achieved with high precision.
SCIENTIFIC REPORTS
(2021)
Article
Engineering, Environmental
Xiaoyang Jiang, Sihui Li, Ken-Ichiro Sotowa, Osamu Tonomura, Tae Hoon Oh
Summary: In this study, continuous synthesis of nanoFe3O4 was achieved within a microchannel using the coprecipitation method in segmented flow. The results, analyzed through X-ray diffraction, scanning electron microscopy, and transmission electron microscopy, showed that the segmented flow synthesis strategy was capable of fabricating approximately 6 nm nanoFe3O4. Moreover, by increasing the high-speed valve frequency and reducing the segment length, uniform nanoparticles were synthesized and the size distribution range was reduced. The developed continuous synthesis method offers significant advantages over existing technologies in terms of productivity and manufacturing cost, providing a promising pathway for industrial-scale continuous synthesis of nanoFe3O4.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Fengtao Jiang, Nan Xiang
Summary: This paper presents the development of a novel handheld sorter with a multifunction integrated microfluidic chip for point-of-care diagnosis in resource-limited settings. The sorter utilizes a spiral inertial microfluidic channel and flow regulators for size-based cell separation. The prototype of the sorter successfully separates malignant tumor cells from clinical pleural effusions. The device is simple to use, inexpensive, portable, and compact, making it suitable for high-throughput label-free cell separation in resource-limited areas.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Minruihong Wang, Hongyan Liang, Xiao Chen, Deyong Chen, Junbo Wang, Yuan Zhang, Jian Chen
Summary: Single-cell analysis plays a crucial role in scientific research and clinical diagnosis, especially in the study of dynamic immune processes and tumor research. The development of optoelectronic flow cytometry technology offers new possibilities for complete blood cell counting and is expected to become the standard for future blood analysis.
Article
Chemistry, Analytical
Boxuan Wu, Xuefeng Xu, Guangyang Li, Xi Yang, Feiya Du, Weiqiang Tan, Jianmin Wang, Shurong Dong, Jikui Luo, Xiaozhi Wang, Zhen Cao
Summary: This paper presents a highly effective microfluidic emulsion system using an integrated MCP for high-throughput production of monodisperse droplets. The system has been validated for various applications, including hydrogel microsphere preparation and absolute quantification of genes. It offers easy fabrication, robust performance, and simple operation, making it suitable for droplet-based assays and tissue engineering applications.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Jialan Cao, David A. Russo, Ting Xie, G. Alexander Gross, Julie A. Z. Zedler
Summary: A droplet-based microfluidic platform was used to systematically optimize cultivation parameters for cyanobacteria. Three different unicellular cyanobacteria were successfully grown, and one-dimensional and two-dimensional screenings were performed to determine key nutrient requirements. The results suggested that nitrate and/or phosphate may be limiting nutrients in standard cultivation media. Improved medium composition led to a 15.7% increase in biomass yield.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
M. S. Wiederoder, E. L. Kendall, J. -H. Han, R. G. Ulrich, D. L. DeVoe
SENSORS AND ACTUATORS B-CHEMICAL
(2018)
Article
Materials Science, Multidisciplinary
Zhu Chen, Jung Yeon Han, Laura Shumate, Renee Fedak, Don L. DeVoe
ADVANCED MATERIALS TECHNOLOGIES
(2019)
Article
Engineering, Electrical & Electronic
Oliver M. Barham, Mona Mirzaeimoghri, Don L. Devoe
IEEE TRANSACTIONS ON POWER ELECTRONICS
(2019)
Article
Nanoscience & Nanotechnology
Jung Y. Han, Michael Wiederoder, Don L. DeVoe
MICROSYSTEMS & NANOENGINEERING
(2019)
Article
Biochemical Research Methods
Hesam Babahosseini, Supriya Padmanabhan, Tom Misteli, Don L. DeVoe
Article
Biochemical Research Methods
S. Padmanabhan, J. Y. Han, I Nanayankkara, K. Tran, P. Ho, N. Mesfin, I White, D. L. DeVoe
Article
Materials Science, Multidisciplinary
Jung Yeon Han, Beqir Krasniqi, Jung Kim, Melissa Keckley, Don L. DeVoe
ADVANCED MATERIALS TECHNOLOGIES
(2020)
Article
Engineering, Biomedical
Jung Y. Han, Don L. DeVoe
Summary: A thermoplastic microfluidic substrate is integrated onto a conventional venipuncture syringe to form a spiral inertial separation element for isolating plasma from diluted whole blood. The system enables plasma separation without the need for external instrumentation, achieving a peak separation efficiency above 97% using 25x diluted blood through Dean flow focusing.
ANNALS OF BIOMEDICAL ENGINEERING
(2021)
Article
Biochemical Research Methods
S. Padmanabhan, A. Sposito, M. Yeh, M. Everitt, I White, D. L. DeVoe
Summary: This study demonstrates the seamless integration of multiple reagents into thermoplastic 2D microwell arrays using a scalable pin spotting technique, allowing for high-resolution deposition of concentrated reagents. The performance of this integration method was successfully characterized, showing potential for future applications in disposable diagnostics.
Article
Engineering, Biomedical
Hesam Babahosseini, Darawalee Wangsa, Mani Pabba, Thomas Ried, Tom Misteli, Don L. DeVoe
Summary: This technology presents selective spatial isolation and manipulation of single chromosomes, controlled formation of defined chromosome ensembles through a droplet-based microfluidic system. By combining discretization, optical interrogation, and selective droplet release, efficient manipulation of multiple chromosomes into a defined ensemble is achieved.
Article
Multidisciplinary Sciences
Jung Yeon Han, Joseph N. La Fiandra, Don L. DeVoe
Summary: This paper presents a microfluidic vortex focusing technique for mass production of liposomes with controlled size and low variability, addressing the trade-offs between size uniformity and mass production in liposome synthesis.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Jung Y. Han, Michael Yeh, Don L. DeVoe
Summary: This paper presents a microfluidic device that enables the isolation and concentration of bacteria for analysis using confocal Raman spectroscopy. The device utilizes a glass-on-silicon structure with a tapered chamber and a surrounding nanogap to concentrate cells at the chamber apex during sample perfusion. The nanogap retains bacteria by size exclusion while allowing smaller contaminants to pass through. Concentrating bacteria within a fixed volume enables the use of single-point confocal Raman detection for rapid spectral analysis and identification. The technology was evaluated for the analysis of three pathogens, showing promising results for label-free bacteria identification.
Article
Materials Science, Multidisciplinary
Jason Harriot, Michael Yeh, Mani Pabba, Don L. DeVoe
Summary: A unique droplet microfluidic technology is presented, which allows programmable deterministic control over complex droplet operations. The system integrates computer vision with a membrane displacement traps array, enabling automated feedback control. Bidirectional flow control is achieved using an H-bridge channel topology, increasing operational flexibility. Overall, this technology combines the advantages of droplet-based fluid manipulation with robotic liquid handling, significantly expanding the potential for diverse biological and biochemical applications.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Proceedings Paper
Engineering, Biomedical
H. Babahosseini, S. Padmanabhan, T. Misteli, D. L. DeVoe
2019 41ST ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC)
(2019)
Article
Biochemical Research Methods
Hesam Babahosseini, Tom Misteli, Don L. DeVoe